Mutagenetix > Incidental Mutation

Incidental Mutation 'R9382:Or51f1'

710104

Institutional Source

Beutler Lab

Gene Symbol

Or51f1

Ensembl Gene

ENSMUSG00000060888

Gene Name

olfactory receptor family 51 subfamily F member 1

Synonyms

MOR14-7P, Olfr566, GA_x6K02T2PBJ9-5560696-5559746

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R9382 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

102505528-102507972 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 102506014 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 158 (I158M)

Ref Sequence

ENSEMBL: ENSMUSP00000148058 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071393] [ENSMUST00000209952] [ENSMUST00000213481]

AlphaFold

A0A1B0GST0

Predicted Effect

probably benign
Transcript: ENSMUST00000071393
AA Change: I155M

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000071344
Gene: ENSMUSG00000060888
AA Change: I155M

Domain	Start	End	E-Value	Type
Pfam:7tm_4	37	316	1e-110	PFAM
Pfam:7TM_GPCR_Srsx	42	313	3.5e-6	PFAM
Pfam:7tm_1	47	298	9.3e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000209952
AA Change: I158M

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

Predicted Effect

probably benign
Transcript: ENSMUST00000213481
AA Change: I158M

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8b	G	A	11: 109,870,711 (GRCm39)	S147L	probably benign	Het
Abraxas1	A	G	5: 100,957,649 (GRCm39)	V190A	probably benign	Het
Aoc1l2	A	T	6: 48,907,298 (GRCm39)	K99N	probably benign	Het
Aox1	T	A	1: 58,104,501 (GRCm39)	H559Q	possibly damaging	Het
Arhgap31	C	T	16: 38,422,988 (GRCm39)	G1026D	probably benign	Het
C1ra	T	C	6: 124,490,819 (GRCm39)	F71L	probably benign	Het
C530025M09Rik	T	C	2: 149,672,640 (GRCm39)	H165R	unknown	Het
Celf2	A	T	2: 6,726,404 (GRCm39)	M43K	probably damaging	Het
Celsr3	T	C	9: 108,706,961 (GRCm39)	V1148A	possibly damaging	Het
Cmya5	T	G	13: 93,229,884 (GRCm39)	K1735Q	probably benign	Het
Cntln	A	C	4: 84,968,318 (GRCm39)	M846L	probably benign	Het
Cntn5	T	C	9: 9,673,817 (GRCm39)	T762A	probably benign	Het
Col11a1	T	A	3: 113,899,046 (GRCm39)	L525Q	unknown	Het
Col12a1	T	C	9: 79,589,364 (GRCm39)	T1064A	probably benign	Het
Cyc1	T	C	15: 76,229,273 (GRCm39)	V211A	possibly damaging	Het
Ddx56	A	G	11: 6,215,516 (GRCm39)	S296P	probably damaging	Het
Deptor	A	T	15: 54,975,798 (GRCm39)		probably benign	Het
Ezh1	C	T	11: 101,094,265 (GRCm39)	R409H	possibly damaging	Het
Fam228b	T	A	12: 4,798,147 (GRCm39)	E190V	probably damaging	Het
Fkbp15	A	T	4: 62,237,210 (GRCm39)	L635H	probably damaging	Het
Frem1	G	A	4: 82,901,622 (GRCm39)	L969F	possibly damaging	Het
Gabrg2	T	C	11: 41,858,433 (GRCm39)	R232G	probably benign	Het
Gpr26	G	A	7: 131,568,963 (GRCm39)	D103N	probably damaging	Het
Grid2ip	T	G	5: 143,361,103 (GRCm39)		probably null	Het
Ints7	T	C	1: 191,351,793 (GRCm39)	V834A	probably damaging	Het
Kcnh7	T	A	2: 62,667,612 (GRCm39)	H309L	probably benign	Het
Mael	T	C	1: 166,053,282 (GRCm39)	E241G	probably damaging	Het
Neb	T	A	2: 52,122,277 (GRCm39)	E584V		Het
Nexn	C	T	3: 151,959,401 (GRCm39)	V23M	probably damaging	Het
Npas1	T	C	7: 16,190,231 (GRCm39)		probably null	Het
Pcdh17	T	G	14: 84,685,522 (GRCm39)	V663G	probably damaging	Het
Pga5	C	T	19: 10,646,897 (GRCm39)	G303S	probably damaging	Het
Poln	T	A	5: 34,164,842 (GRCm39)	K844N	probably damaging	Het
Prkar1b	C	T	5: 139,036,442 (GRCm39)	D227N	probably damaging	Het
Proc	T	A	18: 32,256,336 (GRCm39)	I444F	probably damaging	Het
Ptprn	G	T	1: 75,229,135 (GRCm39)	H791N	probably benign	Het
Ror1	T	C	4: 100,191,709 (GRCm39)	S160P	probably benign	Het
Satb2	T	A	1: 56,870,797 (GRCm39)		probably null	Het
Slc43a3	C	T	2: 84,780,771 (GRCm39)	A332V	probably benign	Het
Sp100	G	A	1: 85,627,336 (GRCm39)	V410M	probably damaging	Het
Srgap2	T	C	1: 131,217,346 (GRCm39)	T989A	probably benign	Het
Ssc5d	T	C	7: 4,930,283 (GRCm39)		probably null	Het
Tll2	A	G	19: 41,116,997 (GRCm39)	Y273H	probably benign	Het
Tub	T	C	7: 108,626,211 (GRCm39)	V295A	possibly damaging	Het
Unc13b	T	A	4: 43,172,512 (GRCm39)	D1113E	unknown	Het
Usp9y	C	A	Y: 1,364,776 (GRCm39)	M1012I	probably benign	Het
Vmn1r238	A	G	18: 3,122,676 (GRCm39)	V246A	probably damaging	Het
Vmn2r88	T	G	14: 51,656,197 (GRCm39)	V802G		Het
Wnt7b	T	C	15: 85,443,175 (GRCm39)	Q76R	probably damaging	Het
Zan	T	C	5: 137,389,917 (GRCm39)	R4852G	unknown	Het
Zfp618	A	T	4: 63,051,258 (GRCm39)	T680S	probably damaging	Het
Zfp784	T	A	7: 5,041,338 (GRCm39)	D25V	unknown	Het

Other mutations in Or51f1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02295:Or51f1	APN	7	102,506,031 (GRCm39)	missense	probably benign
IGL03336:Or51f1	APN	7	102,505,823 (GRCm39)	missense	probably benign	0.38
PIT4382001:Or51f1	UTSW	7	102,505,809 (GRCm39)	missense	probably damaging	1.00
R1337:Or51f1	UTSW	7	102,506,078 (GRCm39)	missense	probably benign	0.03
R1791:Or51f1	UTSW	7	102,505,569 (GRCm39)	nonsense	probably null
R3953:Or51f1	UTSW	7	102,505,824 (GRCm39)	missense	probably damaging	0.97
R3954:Or51f1	UTSW	7	102,505,824 (GRCm39)	missense	probably damaging	0.97
R3955:Or51f1	UTSW	7	102,505,824 (GRCm39)	missense	probably damaging	0.97
R3957:Or51f1	UTSW	7	102,505,824 (GRCm39)	missense	probably damaging	0.97
R4734:Or51f1	UTSW	7	102,506,186 (GRCm39)	missense	probably damaging	0.99
R5182:Or51f1	UTSW	7	102,506,176 (GRCm39)	missense	probably benign	0.07
R5394:Or51f1	UTSW	7	102,505,686 (GRCm39)	missense	probably damaging	1.00
R5559:Or51f1	UTSW	7	102,506,414 (GRCm39)	missense	possibly damaging	0.94
R6023:Or51f1	UTSW	7	102,506,169 (GRCm39)	missense	possibly damaging	0.95
R6310:Or51f1	UTSW	7	102,506,412 (GRCm39)	missense	probably benign	0.01
R7312:Or51f1	UTSW	7	102,505,706 (GRCm39)	missense	probably damaging	1.00
R7493:Or51f1	UTSW	7	102,506,278 (GRCm39)	nonsense	probably null
R8409:Or51f1	UTSW	7	102,506,477 (GRCm39)	missense	probably benign	0.01
R8476:Or51f1	UTSW	7	102,506,152 (GRCm39)	missense	probably benign	0.22
R8747:Or51f1	UTSW	7	102,506,139 (GRCm39)	missense	probably benign	0.05
R8946:Or51f1	UTSW	7	102,505,725 (GRCm39)	nonsense	probably null
R9610:Or51f1	UTSW	7	102,506,147 (GRCm39)	missense	probably damaging	1.00
R9611:Or51f1	UTSW	7	102,506,147 (GRCm39)	missense	probably damaging	1.00
R9625:Or51f1	UTSW	7	102,505,636 (GRCm39)	missense	probably benign	0.01
R9800:Or51f1	UTSW	7	102,506,093 (GRCm39)	missense	probably benign	0.14
Z1177:Or51f1	UTSW	7	102,505,581 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TGCATGGAGTATCTATCCCTGTG -3'
(R):5'- ACCTTGAGAATCCTGTGGTTTCAG -3'

Sequencing Primer
(F):5'- CTATCCCTGTGGTCAGAATGAGATC -3'
(R):5'- CCTGTGGTTTCAGGCAAATG -3'

Posted On

2022-04-18