Mutagenetix > Incidental Mutation

Incidental Mutation 'R9492:Adam25'

717004

Institutional Source

Beutler Lab

Gene Symbol

Adam25

Ensembl Gene

ENSMUSG00000071937

Gene Name

ADAM metallopeptidase domain 25

Synonyms

testase 2

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R9492 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

41205245-41209213 bp(+) (GRCm39)

Type of Mutation

start codon destroyed

DNA Base Change (assembly)

A to T at 41206736 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 1 (M1L)

Ref Sequence

ENSEMBL: ENSMUSP00000094420 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096663]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000096663
AA Change: M1L

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000094420
Gene: ENSMUSG00000071937
AA Change: M1L

Domain	Start	End	E-Value	Type
Pfam:Pep_M12B_propep	53	179	3.4e-21	PFAM
Pfam:Reprolysin_5	220	398	1.6e-16	PFAM
Pfam:Reprolysin_4	220	407	2.5e-13	PFAM
Pfam:Reprolysin	221	410	5.6e-46	PFAM
Pfam:Reprolysin_2	222	399	9.7e-14	PFAM
Pfam:Reprolysin_3	246	366	1e-18	PFAM
DISIN	428	503	3.33e-39	SMART
ACR	504	640	8.95e-74	SMART
transmembrane domain	706	728	N/A	INTRINSIC

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is expressed in a regulated fashion during spermatogenesis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional metalloprotease enzyme. This gene is located adjacent to other ADAM genes on chromosome 8. [provided by RefSeq, May 2016]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110009E18Rik	A	C	1: 120,078,472 (GRCm39)	K43T	probably damaging	Het
Abca12	A	G	1: 71,297,380 (GRCm39)	V2370A	possibly damaging	Het
Abca13	T	A	11: 9,243,667 (GRCm39)	C1843*	probably null	Het
Abr	C	T	11: 76,399,751 (GRCm39)	S82N	probably benign	Het
Acot2	T	C	12: 84,039,384 (GRCm39)	S298P	probably benign	Het
Acrbp	A	G	6: 125,038,062 (GRCm39)	D421G	probably benign	Het
Ano8	A	G	8: 71,934,784 (GRCm39)	V441A	possibly damaging	Het
Aoc1l1	T	C	6: 48,955,540 (GRCm39)	S717P	probably benign	Het
Atg2b	T	C	12: 105,624,549 (GRCm39)	H650R	probably benign	Het
Atp11a	C	T	8: 12,894,490 (GRCm39)	T695I	probably damaging	Het
AU018091	A	G	7: 3,214,023 (GRCm39)	S74P	probably benign	Het
Cadps2	A	G	6: 23,427,238 (GRCm39)	Y597H	probably benign	Het
Ccdc15	C	T	9: 37,215,665 (GRCm39)	E619K	probably damaging	Het
Ceacam11	G	T	7: 17,709,468 (GRCm39)	C222F	probably benign	Het
Cmya5	T	C	13: 93,177,822 (GRCm39)	*3677W	probably null	Het
Col11a1	G	C	3: 114,005,752 (GRCm39)	C1628S	probably benign	Het
Dhx8	T	C	11: 101,654,808 (GRCm39)	I1032T	possibly damaging	Het
Dpp10	T	C	1: 123,281,159 (GRCm39)	D630G	probably damaging	Het
Eci3	C	T	13: 35,143,976 (GRCm39)	G50R	probably benign	Het
Eif2a	A	G	3: 58,448,475 (GRCm39)	T103A	probably benign	Het
Frem1	T	C	4: 82,920,057 (GRCm39)	E432G	probably damaging	Het
Gdnf	C	T	15: 7,840,423 (GRCm39)		probably benign	Het
Hace1	T	A	10: 45,547,230 (GRCm39)	M471K	probably benign	Het
Hydin	A	G	8: 111,326,877 (GRCm39)	T4739A	possibly damaging	Het
Ilf2	A	G	3: 90,394,570 (GRCm39)	I320V	probably benign	Het
Iqgap3	C	A	3: 88,016,176 (GRCm39)	F986L	probably damaging	Het
Klhl21	T	C	4: 152,093,419 (GRCm39)	L7S	probably benign	Het
Lrrc61	G	T	6: 48,545,761 (GRCm39)	E195*	probably null	Het
Maz	G	T	7: 126,622,292 (GRCm39)	A443E	possibly damaging	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Mtor	T	A	4: 148,568,801 (GRCm39)	L1107Q	probably damaging	Het
Nectin3	T	C	16: 46,215,511 (GRCm39)	H494R	probably benign	Het
Nlgn1	G	T	3: 25,488,480 (GRCm39)	Y618*	probably null	Het
Nup98	C	T	7: 101,778,252 (GRCm39)	E1372K	probably benign	Het
Or12e9	A	G	2: 87,201,960 (GRCm39)	N28S	probably benign	Het
Or5af1	T	C	11: 58,722,610 (GRCm39)	V210A	probably benign	Het
Otud6b	A	G	4: 14,818,349 (GRCm39)	I184T	probably damaging	Het
Pcsk6	A	G	7: 65,697,346 (GRCm39)	N891S	probably benign	Het
Pde4a	T	A	9: 21,106,096 (GRCm39)	L237Q	probably damaging	Het
Peg10	CCACATCAGGATCCACATCAGGATGCACATCAGCATCAGGATCCCCATCAGGATGCACATCAGGATCCACATCAGGATGCACATCAG	CCACATCAGGATCCACATCAGGATGCACATCAG	6: 4,756,398 (GRCm39)		probably benign	Het
Perm1	TGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCT	TGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCTGAGCCTGACACGGCTTTGTCTACACCCGCCTCT	4: 156,302,525 (GRCm39)		probably benign	Het
Pou3f1	A	G	4: 124,552,179 (GRCm39)	H227R	possibly damaging	Het
Prss53	G	A	7: 127,488,802 (GRCm39)	R31C	probably damaging	Het
Psmb8	T	A	17: 34,417,435 (GRCm39)	D21E	probably benign	Het
Ptprq	A	G	10: 107,478,813 (GRCm39)	Y1277H	probably damaging	Het
Rexo2	T	C	9: 48,380,176 (GRCm39)	T219A	probably benign	Het
Rfc3	A	G	5: 151,566,411 (GRCm39)	F346S	probably damaging	Het
Rhoq	A	T	17: 87,304,373 (GRCm39)	Q168L		Het
Sgk1	A	G	10: 21,874,096 (GRCm39)	N452S	probably damaging	Het
Slc35f3	A	G	8: 127,048,026 (GRCm39)	K122R	probably damaging	Het
Slc4a2	T	A	5: 24,644,761 (GRCm39)	M1021K	probably benign	Het
Sorcs2	A	G	5: 36,186,484 (GRCm39)	V886A	probably benign	Het
Spata20	T	A	11: 94,374,444 (GRCm39)	M308L	probably damaging	Het
Spen	A	T	4: 141,199,098 (GRCm39)	H3176Q	probably benign	Het
Stat5b	T	A	11: 100,692,361 (GRCm39)	H141L	probably benign	Het
Syne2	T	A	12: 75,995,839 (GRCm39)	H2126Q	possibly damaging	Het
Traj46	A	G	14: 54,409,851 (GRCm39)	T20A		Het
Trim30a	G	T	7: 104,078,330 (GRCm39)	Q249K	probably damaging	Het
Trmt13	A	G	3: 116,388,281 (GRCm39)	Y52H	probably benign	Het
Trpv3	A	G	11: 73,187,267 (GRCm39)	I700V	probably damaging	Het
Tyr	G	A	7: 87,121,704 (GRCm39)	H363Y	probably damaging	Het
Tyr	C	G	7: 87,121,705 (GRCm39)	M362I	possibly damaging	Het
Ubb	G	A	11: 62,442,984 (GRCm39)	V5M	probably damaging	Het
Unc5a	T	C	13: 55,150,288 (GRCm39)	L519P	probably damaging	Het
Uso1	A	G	5: 92,315,191 (GRCm39)	D185G	possibly damaging	Het
Vmn1r228	C	T	17: 20,996,862 (GRCm39)	E219K	probably damaging	Het
Wdr49	A	G	3: 75,240,669 (GRCm39)	V400A	probably damaging	Het
Zfp292	A	G	4: 34,810,794 (GRCm39)	M755T	probably benign	Het

Other mutations in Adam25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01444:Adam25	APN	8	41,207,958 (GRCm39)	missense	probably benign
IGL01977:Adam25	APN	8	41,208,134 (GRCm39)	missense	probably benign	0.00
IGL02098:Adam25	APN	8	41,208,680 (GRCm39)	missense	probably benign	0.12
IGL02233:Adam25	APN	8	41,208,423 (GRCm39)	missense	probably damaging	1.00
IGL02458:Adam25	APN	8	41,206,844 (GRCm39)	missense	probably benign	0.01
IGL02527:Adam25	APN	8	41,206,785 (GRCm39)	missense	possibly damaging	0.78
IGL02632:Adam25	APN	8	41,208,237 (GRCm39)	missense	possibly damaging	0.90
IGL02995:Adam25	APN	8	41,206,760 (GRCm39)	missense	probably benign	0.00
H8786:Adam25	UTSW	8	41,207,261 (GRCm39)	missense	probably benign	0.00
R0062:Adam25	UTSW	8	41,207,829 (GRCm39)	missense	probably damaging	1.00
R0062:Adam25	UTSW	8	41,207,829 (GRCm39)	missense	probably damaging	1.00
R0189:Adam25	UTSW	8	41,208,467 (GRCm39)	missense	probably damaging	1.00
R0505:Adam25	UTSW	8	41,208,261 (GRCm39)	missense	probably damaging	1.00
R0532:Adam25	UTSW	8	41,208,987 (GRCm39)	missense	probably benign	0.00
R0699:Adam25	UTSW	8	41,209,011 (GRCm39)	missense	probably benign
R0972:Adam25	UTSW	8	41,208,168 (GRCm39)	missense	probably damaging	1.00
R1053:Adam25	UTSW	8	41,207,768 (GRCm39)	missense	probably benign	0.30
R1079:Adam25	UTSW	8	41,208,513 (GRCm39)	missense	possibly damaging	0.87
R1872:Adam25	UTSW	8	41,208,263 (GRCm39)	nonsense	probably null
R1933:Adam25	UTSW	8	41,207,922 (GRCm39)	missense	probably benign	0.01
R1934:Adam25	UTSW	8	41,207,922 (GRCm39)	missense	probably benign	0.01
R4061:Adam25	UTSW	8	41,206,819 (GRCm39)	missense	possibly damaging	0.67
R4702:Adam25	UTSW	8	41,207,163 (GRCm39)	missense	probably damaging	1.00
R4703:Adam25	UTSW	8	41,207,163 (GRCm39)	missense	probably damaging	1.00
R4705:Adam25	UTSW	8	41,207,163 (GRCm39)	missense	probably damaging	1.00
R4859:Adam25	UTSW	8	41,207,580 (GRCm39)	missense	probably benign	0.01
R5015:Adam25	UTSW	8	41,207,671 (GRCm39)	missense	probably benign	0.22
R5249:Adam25	UTSW	8	41,208,991 (GRCm39)	missense	probably benign
R5628:Adam25	UTSW	8	41,208,747 (GRCm39)	missense	probably benign	0.00
R5791:Adam25	UTSW	8	41,207,257 (GRCm39)	missense	probably benign
R6439:Adam25	UTSW	8	41,207,627 (GRCm39)	missense	possibly damaging	0.92
R6693:Adam25	UTSW	8	41,207,568 (GRCm39)	missense	probably damaging	1.00
R7041:Adam25	UTSW	8	41,207,121 (GRCm39)	missense	probably benign	0.04
R7101:Adam25	UTSW	8	41,208,438 (GRCm39)	missense	probably benign	0.00
R7531:Adam25	UTSW	8	41,206,914 (GRCm39)	missense	probably damaging	0.99
R7600:Adam25	UTSW	8	41,208,854 (GRCm39)	missense	probably benign	0.01
R7634:Adam25	UTSW	8	41,207,883 (GRCm39)	missense	probably benign	0.00
R7964:Adam25	UTSW	8	41,208,576 (GRCm39)	missense	probably damaging	0.99
R8017:Adam25	UTSW	8	41,207,124 (GRCm39)	missense	possibly damaging	0.56
R8021:Adam25	UTSW	8	41,207,796 (GRCm39)	missense	probably damaging	1.00
R8499:Adam25	UTSW	8	41,208,189 (GRCm39)	missense	probably damaging	1.00
R8686:Adam25	UTSW	8	41,208,521 (GRCm39)	missense	probably benign	0.44
R8715:Adam25	UTSW	8	41,207,099 (GRCm39)	missense	probably benign	0.00
R8847:Adam25	UTSW	8	41,206,746 (GRCm39)	missense	probably benign
R8921:Adam25	UTSW	8	41,207,710 (GRCm39)	nonsense	probably null
R9120:Adam25	UTSW	8	41,209,141 (GRCm39)	utr 3 prime	probably benign
R9158:Adam25	UTSW	8	41,208,645 (GRCm39)	missense	probably damaging	1.00
R9339:Adam25	UTSW	8	41,206,911 (GRCm39)	missense	probably damaging	1.00
R9348:Adam25	UTSW	8	41,208,953 (GRCm39)	missense	probably benign
R9454:Adam25	UTSW	8	41,207,486 (GRCm39)	missense	probably damaging	0.99
R9680:Adam25	UTSW	8	41,208,239 (GRCm39)	missense	probably damaging	1.00
RF006:Adam25	UTSW	8	41,208,834 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- AGAGTCATGCCTTTGAACAAACC -3'
(R):5'- TAGGTCAGCCATCCCATAGC -3'

Sequencing Primer
(F):5'- CTTTGAACAAACCTCAGCCATTTAAG -3'
(R):5'- AAGGGTATCACTACTTCTGGGAG -3'

Posted On

2022-07-18