Incidental Mutation 'R9680:Adam25'
ID 728488
Institutional Source Beutler Lab
Gene Symbol Adam25
Ensembl Gene ENSMUSG00000071937
Gene Name ADAM metallopeptidase domain 25
Synonyms testase 2
MMRRC Submission
Accession Numbers
Essential gene? Probably non essential (E-score: 0.097) question?
Stock # R9680 (G1)
Quality Score 225.009
Status Not validated
Chromosome 8
Chromosomal Location 41205245-41209213 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 41208239 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Tyrosine to Asparagine at position 502 (Y502N)
Ref Sequence ENSEMBL: ENSMUSP00000094420 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000096663]
AlphaFold no structure available at present
Predicted Effect probably damaging
Transcript: ENSMUST00000096663
AA Change: Y502N

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000094420
Gene: ENSMUSG00000071937
AA Change: Y502N

DomainStartEndE-ValueType
Pfam:Pep_M12B_propep 53 179 3.4e-21 PFAM
Pfam:Reprolysin_5 220 398 1.6e-16 PFAM
Pfam:Reprolysin_4 220 407 2.5e-13 PFAM
Pfam:Reprolysin 221 410 5.6e-46 PFAM
Pfam:Reprolysin_2 222 399 9.7e-14 PFAM
Pfam:Reprolysin_3 246 366 1e-18 PFAM
DISIN 428 503 3.33e-39 SMART
ACR 504 640 8.95e-74 SMART
transmembrane domain 706 728 N/A INTRINSIC
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 99.9%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency
MGI Phenotype FUNCTION: This gene encodes a member of a disintegrin and metalloprotease (ADAM) family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. This gene is expressed in a regulated fashion during spermatogenesis. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional metalloprotease enzyme. This gene is located adjacent to other ADAM genes on chromosome 8. [provided by RefSeq, May 2016]
Allele List at MGI
Other mutations in this stock
Total: 73 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam6a T A 12: 113,509,484 (GRCm39) L619* probably null Het
Akap9 A T 5: 4,011,587 (GRCm39) R763S probably benign Het
Akt3 G A 1: 176,958,639 (GRCm39) P24S probably damaging Het
Ano2 A T 6: 125,857,382 (GRCm39) probably null Het
B4galnt4 G A 7: 140,647,957 (GRCm39) R491Q possibly damaging Het
Bahcc1 A G 11: 120,163,286 (GRCm39) D528G possibly damaging Het
Camk1g G T 1: 193,030,483 (GRCm39) Q409K probably benign Het
Cdh3 T A 8: 107,274,396 (GRCm39) N638K probably benign Het
Cgnl1 T A 9: 71,562,632 (GRCm39) E882D possibly damaging Het
Cplane1 A G 15: 8,231,785 (GRCm39) N1077S possibly damaging Het
Cpxm2 C T 7: 131,661,651 (GRCm39) E379K probably damaging Het
Cyp3a16 A G 5: 145,389,690 (GRCm39) L225P probably damaging Het
Dchs1 T C 7: 105,411,625 (GRCm39) E1497G probably damaging Het
Denr T A 5: 124,065,117 (GRCm39) S157T possibly damaging Het
Disp3 A G 4: 148,356,101 (GRCm39) I253T probably damaging Het
Dlgap2 C T 8: 14,896,653 (GRCm39) T1043M probably damaging Het
Epb41l5 T C 1: 119,535,804 (GRCm39) T355A probably damaging Het
Etv6 G A 6: 134,013,062 (GRCm39) probably benign Het
Fbn1 T C 2: 125,310,484 (GRCm39) I141V probably benign Het
Fsip2 T G 2: 82,819,272 (GRCm39) S5002A probably benign Het
Gbf1 T G 19: 46,271,837 (GRCm39) V1576G probably damaging Het
Gmfg T A 7: 28,140,733 (GRCm39) probably null Het
Gsdmc4 T C 15: 63,774,706 (GRCm39) E25G possibly damaging Het
Igkv6-23 A T 6: 70,237,884 (GRCm39) L12M possibly damaging Het
Kank1 G A 19: 25,388,138 (GRCm39) V604M probably damaging Het
Kctd1 A G 18: 15,140,822 (GRCm39) V40A probably damaging Het
Krt222 T C 11: 99,127,065 (GRCm39) K185R possibly damaging Het
Ldc1 A G 4: 130,115,527 (GRCm39) V7A probably benign Het
Lipm T C 19: 34,089,494 (GRCm39) F151L probably damaging Het
Lrrc2 A T 9: 110,791,710 (GRCm39) N154I probably damaging Het
Map1a T A 2: 121,132,865 (GRCm39) M1227K probably damaging Het
Med1 T C 11: 98,071,114 (GRCm39) T78A probably damaging Het
Mrm1 A G 11: 84,710,144 (GRCm39) S19P possibly damaging Het
Naf1 GCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAACTGGGATGCGGGCGGAAGACCACCACCGCCGCCAGCCCCGAACTCGGATCCCGGCGGAAGACC GCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCTGCCAGCCCCGAACTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAGCTCGGATCCCGGCGGAAGACCACCGCCGCCGCCAGCCCCGAACTGGGATGCGGGCGGAAGACCACCACCGCCGCCAGCCCCGAACTCGGATCCCGGCGGAAGACC 8: 67,313,200 (GRCm39) probably benign Het
Nap1l1 A G 10: 111,330,657 (GRCm39) Y354C probably damaging Het
Npr1 A G 3: 90,368,448 (GRCm39) V474A probably benign Het
Nyap1 A T 5: 137,733,840 (GRCm39) S398T probably damaging Het
Obox7 C T 7: 14,398,067 (GRCm39) Q36* probably null Het
Or5aq6 A T 2: 86,923,390 (GRCm39) M117K possibly damaging Het
Or6c8 A T 10: 128,915,358 (GRCm39) I158N probably benign Het
P3h1 T A 4: 119,090,428 (GRCm39) L92H probably benign Het
Pappa2 T C 1: 158,609,818 (GRCm39) T1548A possibly damaging Het
Pnisr A G 4: 21,873,586 (GRCm39) E443G probably damaging Het
Prdm2 A G 4: 142,859,079 (GRCm39) S1404P possibly damaging Het
Prom1 A T 5: 44,190,284 (GRCm39) probably null Het
Rbm27 A T 18: 42,455,186 (GRCm39) Q646H probably damaging Het
Scamp3 C T 3: 89,087,561 (GRCm39) R134* probably null Het
Scrt2 T C 2: 151,924,018 (GRCm39) C17R probably benign Het
Setbp1 A G 18: 78,902,498 (GRCm39) S390P probably benign Het
Shank2 A T 7: 143,964,837 (GRCm39) D815V probably damaging Het
Sirt7 G A 11: 120,511,296 (GRCm39) T285M Het
Sis T G 3: 72,863,621 (GRCm39) S206R probably benign Het
Slc24a4 T G 12: 102,193,334 (GRCm39) D206E possibly damaging Het
Slc26a4 C A 12: 31,585,292 (GRCm39) G501C probably damaging Het
Sptb T C 12: 76,677,489 (GRCm39) N115S probably damaging Het
Srprb T C 9: 103,074,807 (GRCm39) T112A possibly damaging Het
Tbk1 G T 10: 121,389,841 (GRCm39) A537E probably benign Het
Tbx19 T A 1: 164,970,067 (GRCm39) H274L probably damaging Het
Tmem130 A G 5: 144,674,233 (GRCm39) S394P probably damaging Het
Tmem40 A G 6: 115,718,517 (GRCm39) S104P possibly damaging Het
Tmprss2 T A 16: 97,379,826 (GRCm39) Q158L probably damaging Het
Tnip1 A T 11: 54,828,876 (GRCm39) V97D possibly damaging Het
Tpr T A 1: 150,314,887 (GRCm39) S1985T probably benign Het
Traf7 CA CAA 17: 24,746,737 (GRCm39) probably benign Het
Trio G T 15: 27,744,158 (GRCm39) N2591K possibly damaging Het
Ushbp1 A T 8: 71,838,573 (GRCm39) C618S possibly damaging Het
Usp34 A G 11: 23,317,385 (GRCm39) S834G possibly damaging Het
Vmn2r103 C A 17: 20,019,525 (GRCm39) C536* probably null Het
Vmn2r124 T A 17: 18,293,758 (GRCm39) V615D probably damaging Het
Zbtb38 G A 9: 96,570,397 (GRCm39) T229I probably benign Het
Zfhx4 T A 3: 5,465,656 (GRCm39) V1963E probably damaging Het
Zfp142 G A 1: 74,610,933 (GRCm39) T954M probably benign Het
Zgrf1 A G 3: 127,409,216 (GRCm39) Y1730C probably benign Het
Other mutations in Adam25
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01444:Adam25 APN 8 41,207,958 (GRCm39) missense probably benign
IGL01977:Adam25 APN 8 41,208,134 (GRCm39) missense probably benign 0.00
IGL02098:Adam25 APN 8 41,208,680 (GRCm39) missense probably benign 0.12
IGL02233:Adam25 APN 8 41,208,423 (GRCm39) missense probably damaging 1.00
IGL02458:Adam25 APN 8 41,206,844 (GRCm39) missense probably benign 0.01
IGL02527:Adam25 APN 8 41,206,785 (GRCm39) missense possibly damaging 0.78
IGL02632:Adam25 APN 8 41,208,237 (GRCm39) missense possibly damaging 0.90
IGL02995:Adam25 APN 8 41,206,760 (GRCm39) missense probably benign 0.00
H8786:Adam25 UTSW 8 41,207,261 (GRCm39) missense probably benign 0.00
R0062:Adam25 UTSW 8 41,207,829 (GRCm39) missense probably damaging 1.00
R0062:Adam25 UTSW 8 41,207,829 (GRCm39) missense probably damaging 1.00
R0189:Adam25 UTSW 8 41,208,467 (GRCm39) missense probably damaging 1.00
R0505:Adam25 UTSW 8 41,208,261 (GRCm39) missense probably damaging 1.00
R0532:Adam25 UTSW 8 41,208,987 (GRCm39) missense probably benign 0.00
R0699:Adam25 UTSW 8 41,209,011 (GRCm39) missense probably benign
R0972:Adam25 UTSW 8 41,208,168 (GRCm39) missense probably damaging 1.00
R1053:Adam25 UTSW 8 41,207,768 (GRCm39) missense probably benign 0.30
R1079:Adam25 UTSW 8 41,208,513 (GRCm39) missense possibly damaging 0.87
R1872:Adam25 UTSW 8 41,208,263 (GRCm39) nonsense probably null
R1933:Adam25 UTSW 8 41,207,922 (GRCm39) missense probably benign 0.01
R1934:Adam25 UTSW 8 41,207,922 (GRCm39) missense probably benign 0.01
R4061:Adam25 UTSW 8 41,206,819 (GRCm39) missense possibly damaging 0.67
R4702:Adam25 UTSW 8 41,207,163 (GRCm39) missense probably damaging 1.00
R4703:Adam25 UTSW 8 41,207,163 (GRCm39) missense probably damaging 1.00
R4705:Adam25 UTSW 8 41,207,163 (GRCm39) missense probably damaging 1.00
R4859:Adam25 UTSW 8 41,207,580 (GRCm39) missense probably benign 0.01
R5015:Adam25 UTSW 8 41,207,671 (GRCm39) missense probably benign 0.22
R5249:Adam25 UTSW 8 41,208,991 (GRCm39) missense probably benign
R5628:Adam25 UTSW 8 41,208,747 (GRCm39) missense probably benign 0.00
R5791:Adam25 UTSW 8 41,207,257 (GRCm39) missense probably benign
R6439:Adam25 UTSW 8 41,207,627 (GRCm39) missense possibly damaging 0.92
R6693:Adam25 UTSW 8 41,207,568 (GRCm39) missense probably damaging 1.00
R7041:Adam25 UTSW 8 41,207,121 (GRCm39) missense probably benign 0.04
R7101:Adam25 UTSW 8 41,208,438 (GRCm39) missense probably benign 0.00
R7531:Adam25 UTSW 8 41,206,914 (GRCm39) missense probably damaging 0.99
R7600:Adam25 UTSW 8 41,208,854 (GRCm39) missense probably benign 0.01
R7634:Adam25 UTSW 8 41,207,883 (GRCm39) missense probably benign 0.00
R7964:Adam25 UTSW 8 41,208,576 (GRCm39) missense probably damaging 0.99
R8017:Adam25 UTSW 8 41,207,124 (GRCm39) missense possibly damaging 0.56
R8021:Adam25 UTSW 8 41,207,796 (GRCm39) missense probably damaging 1.00
R8499:Adam25 UTSW 8 41,208,189 (GRCm39) missense probably damaging 1.00
R8686:Adam25 UTSW 8 41,208,521 (GRCm39) missense probably benign 0.44
R8715:Adam25 UTSW 8 41,207,099 (GRCm39) missense probably benign 0.00
R8847:Adam25 UTSW 8 41,206,746 (GRCm39) missense probably benign
R8921:Adam25 UTSW 8 41,207,710 (GRCm39) nonsense probably null
R9120:Adam25 UTSW 8 41,209,141 (GRCm39) utr 3 prime probably benign
R9158:Adam25 UTSW 8 41,208,645 (GRCm39) missense probably damaging 1.00
R9339:Adam25 UTSW 8 41,206,911 (GRCm39) missense probably damaging 1.00
R9348:Adam25 UTSW 8 41,208,953 (GRCm39) missense probably benign
R9454:Adam25 UTSW 8 41,207,486 (GRCm39) missense probably damaging 0.99
R9492:Adam25 UTSW 8 41,206,736 (GRCm39) start codon destroyed probably benign 0.12
RF006:Adam25 UTSW 8 41,208,834 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- AAAGCTGTGCAGATGATCCATG -3'
(R):5'- AGGATGTCTGGGTCATGACATC -3'

Sequencing Primer
(F):5'- TGCAGATGATCCATGCTGTAAGC -3'
(R):5'- CTGGGTCATGACATCTTAAGTATGC -3'
Posted On 2022-10-06