Mutagenetix > Incidental Mutation

Incidental Mutation 'R9605:Zfp84'

726426

Institutional Source

Beutler Lab

Gene Symbol

Zfp84

Ensembl Gene

ENSMUSG00000046185

Gene Name

zinc finger protein 84

Synonyms

KRAB18, C86188, Zfp69, 2210410P13Rik, 4633401C23Rik

MMRRC Submission

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R9605 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

29467977-29479246 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 29476264 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 319 (T319A)

Ref Sequence

ENSEMBL: ENSMUSP00000032802 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000032802]

AlphaFold

Q9D654

Predicted Effect

possibly damaging
Transcript: ENSMUST00000032802
AA Change: T319A

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000032802
Gene: ENSMUSG00000046185
AA Change: T319A

Domain	Start	End	E-Value	Type
KRAB	14	74	9.09e-36	SMART
ZnF_C2H2	249	271	1.67e-2	SMART
ZnF_C2H2	277	299	1.43e-1	SMART
ZnF_C2H2	305	327	5.81e-2	SMART
ZnF_C2H2	333	355	1.95e-3	SMART
ZnF_C2H2	361	383	8.6e-5	SMART
ZnF_C2H2	389	411	2.32e-1	SMART
ZnF_C2H2	417	439	3.89e-3	SMART
ZnF_C2H2	445	467	1.69e-3	SMART
ZnF_C2H2	473	495	9.58e-3	SMART
ZnF_C2H2	501	523	1.38e-3	SMART
ZnF_C2H2	529	551	1.58e-3	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 94 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
6430548M08Rik	G	A	8: 120,872,380 (GRCm39)	V103I	possibly damaging	Het
Aatk	T	C	11: 119,902,209 (GRCm39)	E729G	possibly damaging	Het
Acaa2	A	G	18: 74,932,230 (GRCm39)	T290A	probably benign	Het
Acaca	C	T	11: 84,183,842 (GRCm39)	T1240I	probably benign	Het
Adgre1	A	G	17: 57,718,083 (GRCm39)	N365S	probably benign	Het
Adipor1	A	G	1: 134,352,553 (GRCm39)	D108G	probably damaging	Het
Aebp2	A	G	6: 140,593,736 (GRCm39)	Q462R	probably damaging	Het
Afg2a	C	T	3: 37,505,930 (GRCm39)	P670S	probably damaging	Het
Arhgef4	A	G	1: 34,761,745 (GRCm39)	T334A	unknown	Het
Art5	T	C	7: 101,746,412 (GRCm39)	E280G	probably benign	Het
Bloc1s3	A	G	7: 19,241,457 (GRCm39)	S24P	possibly damaging	Het
C2	T	A	17: 35,081,958 (GRCm39)	N720I	possibly damaging	Het
Caprin2	T	C	6: 148,744,332 (GRCm39)	D1031G	probably damaging	Het
Catsper1	A	G	19: 5,387,785 (GRCm39)	T355A	probably benign	Het
Cd19	T	A	7: 126,010,057 (GRCm39)	E398D	possibly damaging	Het
Cdca8	T	C	4: 124,830,384 (GRCm39)	E31G	probably damaging	Het
Ceacam5	G	A	7: 17,493,520 (GRCm39)	V848M	probably damaging	Het
Chd8	A	T	14: 52,457,055 (GRCm39)	L971Q	probably damaging	Het
Ciao1	G	A	2: 127,087,684 (GRCm39)	T217I	probably damaging	Het
Cldn1	A	T	16: 26,181,924 (GRCm39)	I95N	probably damaging	Het
Clip2	C	T	5: 134,533,616 (GRCm39)	R487Q	probably benign	Het
Cluh	C	T	11: 74,558,772 (GRCm39)	R1253C	possibly damaging	Het
Cngb3	A	T	4: 19,505,187 (GRCm39)	Q640L	probably benign	Het
Cspg4b	T	C	13: 113,456,503 (GRCm39)	S850P		Het
Cyp2c40	A	T	19: 39,766,443 (GRCm39)	V384D	probably damaging	Het
Cyp2d26	T	A	15: 82,674,672 (GRCm39)	M437L	probably benign	Het
D430041D05Rik	A	G	2: 104,087,189 (GRCm39)	S596P	probably benign	Het
Dagla	A	T	19: 10,233,053 (GRCm39)	V448D	probably damaging	Het
Ddx59	A	T	1: 136,344,594 (GRCm39)	E88D	probably benign	Het
Dock1	T	A	7: 134,384,141 (GRCm39)	F671I	possibly damaging	Het
Dtna	T	A	18: 23,764,454 (GRCm39)	V541D	probably damaging	Het
Eef2k	T	C	7: 120,491,170 (GRCm39)	F552S	probably damaging	Het
Erbb2	T	C	11: 98,311,746 (GRCm39)	V94A	possibly damaging	Het
Etl4	A	T	2: 20,771,345 (GRCm39)	I607F	possibly damaging	Het
Ets2	G	T	16: 95,516,121 (GRCm39)	E234*	probably null	Het
Fbp1	A	C	13: 63,019,023 (GRCm39)	V175G	probably damaging	Het
Fmn2	C	T	1: 174,436,194 (GRCm39)	Q722*	probably null	Het
Fnip1	G	T	11: 54,381,713 (GRCm39)	R288L	probably benign	Het
Ghr	G	A	15: 3,362,993 (GRCm39)	P160S	probably damaging	Het
Glipr1	A	G	10: 111,832,801 (GRCm39)	S46P	probably damaging	Het
Kif19b	A	T	5: 140,455,461 (GRCm39)	T356S	probably benign	Het
Kif9	G	A	9: 110,346,710 (GRCm39)	R616H	probably benign	Het
Krt87	G	T	15: 101,336,484 (GRCm39)	C56*	probably null	Het
Ldlr	A	G	9: 21,646,626 (GRCm39)	D264G	probably damaging	Het
Lrrk2	A	T	15: 91,621,420 (GRCm39)	D998V	probably benign	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Mrc1	A	G	2: 14,324,110 (GRCm39)	N1149S	probably benign	Het
Myo1c	T	A	11: 75,559,899 (GRCm39)	V661E	probably benign	Het
N4bp2	T	C	5: 65,963,879 (GRCm39)	S643P	probably benign	Het
Nbl1	T	A	4: 138,812,608 (GRCm39)	T75S	probably benign	Het
Ndn	C	T	7: 61,998,337 (GRCm39)	P61L	possibly damaging	Het
Nqo2	T	A	13: 34,156,361 (GRCm39)	V25E	possibly damaging	Het
Oma1	G	T	4: 103,210,726 (GRCm39)	V411L	possibly damaging	Het
Or12e13	T	G	2: 87,663,478 (GRCm39)	F32V	probably benign	Het
Or5v1	T	A	17: 37,810,331 (GRCm39)	I263N	probably damaging	Het
Or8b12i	A	G	9: 20,082,093 (GRCm39)	V258A	probably damaging	Het
Osgin2	G	T	4: 15,998,427 (GRCm39)	H398Q	probably damaging	Het
Pepd	A	C	7: 34,743,218 (GRCm39)	D419A	probably benign	Het
Pikfyve	A	T	1: 65,303,561 (GRCm39)	S1694C	probably benign	Het
Pirb	G	A	7: 3,720,617 (GRCm39)	R294C	possibly damaging	Het
Plec	A	T	15: 76,065,213 (GRCm39)	L1619Q	unknown	Het
Polq	A	G	16: 36,843,173 (GRCm39)	I236V	probably benign	Het
Pphln1-ps1	A	G	16: 13,495,087 (GRCm39)	D62G	probably benign	Het
Prkag3	T	G	1: 74,786,378 (GRCm39)	Q189P	probably damaging	Het
Prkcg	A	T	7: 3,359,360 (GRCm39)	M136L	probably benign	Het
Ptprn2	T	C	12: 117,125,278 (GRCm39)	L604P	probably benign	Het
Rab6b	A	T	9: 103,017,601 (GRCm39)	T31S	probably benign	Het
Rnf213	A	G	11: 119,359,879 (GRCm39)	N4424S		Het
Ryr3	A	T	2: 112,491,966 (GRCm39)	L3795Q	probably damaging	Het
Scg2	A	G	1: 79,412,936 (GRCm39)	Y556H	probably damaging	Het
Serpina3j	A	G	12: 104,286,093 (GRCm39)	N416S	probably damaging	Het
Sik3	T	A	9: 46,120,117 (GRCm39)	H735Q	probably benign	Het
Slain1	A	C	14: 103,902,112 (GRCm39)	T60P		Het
Slc14a1	C	A	18: 78,152,807 (GRCm39)	A367S	probably damaging	Het
Slc46a2	A	T	4: 59,914,056 (GRCm39)	V289E	probably damaging	Het
Sorcs3	A	T	19: 48,711,364 (GRCm39)	Y643F	probably damaging	Het
Sox30	A	G	11: 45,875,640 (GRCm39)	N464S	possibly damaging	Het
Spta1	A	G	1: 174,035,880 (GRCm39)	Y1062C	probably damaging	Het
Srrm3	A	G	5: 135,881,105 (GRCm39)	D135G	probably damaging	Het
Stat5b	T	A	11: 100,699,276 (GRCm39)	H25L	possibly damaging	Het
Synj2	A	G	17: 6,063,794 (GRCm39)	I513V	probably benign	Het
Syt11	T	A	3: 88,669,325 (GRCm39)	Q189L	probably benign	Het
Tbc1d1	G	A	5: 64,443,350 (GRCm39)	R523Q	probably damaging	Het
Traf3ip2	T	A	10: 39,521,772 (GRCm39)	D443E	probably benign	Het
Traf6	G	T	2: 101,524,625 (GRCm39)	C235F	probably damaging	Het
Treh	A	G	9: 44,592,416 (GRCm39)	D47G	probably damaging	Het
Trpc4	C	A	3: 54,225,550 (GRCm39)	H966Q	probably benign	Het
Ttbk1	A	T	17: 46,784,516 (GRCm39)	D316E	possibly damaging	Het
Txndc16	A	T	14: 45,442,799 (GRCm39)	F132Y	probably damaging	Het
Uggt1	A	T	1: 36,273,886 (GRCm39)		probably null	Het
Usp25	A	T	16: 76,874,046 (GRCm39)	I541F	probably damaging	Het
Xirp1	A	T	9: 119,847,274 (GRCm39)	D536E	possibly damaging	Het
Zfp51	A	G	17: 21,684,291 (GRCm39)	E302G	probably damaging	Het
Zfp648	A	T	1: 154,080,110 (GRCm39)	T90S	probably benign	Het

Other mutations in Zfp84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01768:Zfp84	APN	7	29,476,091 (GRCm39)	missense	probably benign	0.16
IGL03022:Zfp84	APN	7	29,474,759 (GRCm39)	splice site	probably benign
R0666:Zfp84	UTSW	7	29,476,276 (GRCm39)	missense	probably damaging	1.00
R0781:Zfp84	UTSW	7	29,470,797 (GRCm39)	start codon destroyed	probably null	0.02
R1110:Zfp84	UTSW	7	29,470,797 (GRCm39)	start codon destroyed	probably null	0.02
R1353:Zfp84	UTSW	7	29,475,600 (GRCm39)	missense	probably benign	0.02
R1495:Zfp84	UTSW	7	29,476,728 (GRCm39)	nonsense	probably null
R1496:Zfp84	UTSW	7	29,476,039 (GRCm39)	missense	possibly damaging	0.53
R1681:Zfp84	UTSW	7	29,476,825 (GRCm39)	missense	probably damaging	1.00
R1827:Zfp84	UTSW	7	29,476,768 (GRCm39)	missense	possibly damaging	0.91
R1854:Zfp84	UTSW	7	29,474,796 (GRCm39)	missense	possibly damaging	0.84
R2209:Zfp84	UTSW	7	29,476,607 (GRCm39)	missense	probably damaging	0.99
R2843:Zfp84	UTSW	7	29,474,758 (GRCm39)	splice site	probably null
R2844:Zfp84	UTSW	7	29,474,758 (GRCm39)	splice site	probably null
R4691:Zfp84	UTSW	7	29,476,505 (GRCm39)	missense	probably damaging	1.00
R5453:Zfp84	UTSW	7	29,475,722 (GRCm39)	missense	possibly damaging	0.82
R5474:Zfp84	UTSW	7	29,476,514 (GRCm39)	missense	probably damaging	1.00
R5578:Zfp84	UTSW	7	29,474,856 (GRCm39)	missense	possibly damaging	0.93
R5646:Zfp84	UTSW	7	29,475,818 (GRCm39)	missense	probably benign	0.05
R5963:Zfp84	UTSW	7	29,476,378 (GRCm39)	missense	probably damaging	1.00
R6830:Zfp84	UTSW	7	29,475,911 (GRCm39)	missense	probably benign	0.00
R8129:Zfp84	UTSW	7	29,475,862 (GRCm39)	missense	probably benign	0.00
R8138:Zfp84	UTSW	7	29,474,797 (GRCm39)	missense	probably damaging	0.99
R9180:Zfp84	UTSW	7	29,474,873 (GRCm39)	missense	probably damaging	0.97
R9401:Zfp84	UTSW	7	29,476,297 (GRCm39)	missense	probably damaging	1.00
R9489:Zfp84	UTSW	7	29,476,264 (GRCm39)	missense	possibly damaging	0.89
R9555:Zfp84	UTSW	7	29,476,102 (GRCm39)	missense	probably damaging	0.99
V3553:Zfp84	UTSW	7	29,476,672 (GRCm39)	missense	probably benign	0.36
Z1186:Zfp84	UTSW	7	29,470,805 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- AGCACGCTTACCTTGTTCAG -3'
(R):5'- GAAAGGCCTTTTCGCAGTAG -3'

Sequencing Primer
(F):5'- GCATAACCGCTTTCACACGGG -3'
(R):5'- TCGCAGTAGTTACACTCGTAAGG -3'

Posted On

2022-10-06