Phenotypic Mutation 'panda' (pdf version)
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Allelepanda
Mutation Type nonsense
Chromosome15
Coordinate94,326,699 bp (GRCm38)
Base Change G ⇒ T (forward strand)
Gene Adamts20
Gene Name a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 20
Synonym(s) ADAMTS-20, bt
Chromosomal Location 94,270,163-94,465,418 bp (-)
MGI Phenotype FUNCTION: This gene encodes a member of "a disintegrin and metalloproteinase with thrombospondin motifs" (ADAMTS) family of multi-domain matrix-associated metalloendopeptidases that have diverse roles in tissue morphogenesis and pathophysiological remodeling, in inflammation and in vascular biology. The encoded preproprotein undergoes proteolytic processing to generate an active protease. Certain mutations in this gene cause defective development of neural crest-derived melanoblasts resulting in a "belted" phenotype that is characterized by white spots in the lumbar region. [provided by RefSeq, Jul 2016]
PHENOTYPE: Mice homozygous for spontaneous or ENU-induced mutations exhibit abnormal coat/hair pigmentation, including a typical white belt phenotype. [provided by MGI curators]
Accession Number

NCBI RefSeq: NM_177431; MGI: 2660628

Mapped Yes 
Amino Acid Change Serine changed to Stop codon
Institutional SourceBeutler Lab
Ref Sequences
S566* in Ensembl: ENSMUSP00000036330 (fasta)
Gene Model not available
SMART Domains

DomainStartEndE-ValueType
Pfam:Pep_M12B_propep 10 186 3.2e-33 PFAM
Pfam:Reprolysin 255 464 1.2e-26 PFAM
Blast:ACR 467 545 N/A BLAST
TSP1 556 608 7.73e-11 SMART
Pfam:ADAM_spacer1 718 836 6.2e-33 PFAM
TSP1 846 901 1.47e-1 SMART
TSP1 904 958 2.83e0 SMART
TSP1 965 1019 4.28e-4 SMART
TSP1 1020 1074 1.89e-5 SMART
TSP1 1075 1131 4.87e-8 SMART
TSP1 1152 1201 6.05e-4 SMART
TSP1 1204 1260 1.22e-8 SMART
TSP1 1304 1356 1.37e-2 SMART
TSP1 1357 1411 6e-8 SMART
TSP1 1416 1470 1.69e-2 SMART
TSP1 1471 1526 2.3e0 SMART
TSP1 1530 1579 1.23e0 SMART
TSP1 1653 1706 5.27e-4 SMART
Pfam:GON 1707 1906 1.1e-87 PFAM
Phenotypic Category
Phenotypequestion? Literature verified References
pigmentation
skin/coat/nails
Penetrance Probably greater than 90%, but with variable expressivity. 
Alleles Listed at MGI

All alleles(16) : Spontaneous(11) Chemically induced(5)

Lab Alleles
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00433:Adamts20 APN 15 94394641 missense probably benign
IGL00491:Adamts20 APN 15 94273232 missense possibly damaging 0.89
IGL00502:Adamts20 APN 15 94403397 missense probably damaging 0.99
IGL00672:Adamts20 APN 15 94341105 missense probably damaging 0.99
IGL00840:Adamts20 APN 15 94282482 missense probably damaging 1.00
IGL00909:Adamts20 APN 15 94379813 missense probably damaging 1.00
IGL01101:Adamts20 APN 15 94344042 missense probably damaging 1.00
IGL01137:Adamts20 APN 15 94394611 critical splice donor site probably null
IGL01457:Adamts20 APN 15 94331448 missense probably damaging 0.97
IGL01685:Adamts20 APN 15 94403446 missense possibly damaging 0.81
IGL01949:Adamts20 APN 15 94326106 missense probably benign 0.08
IGL02525:Adamts20 APN 15 94283078 splice site probably null
IGL03088:Adamts20 APN 15 94329914 critical splice donor site probably null
IGL03175:Adamts20 APN 15 94273255 nonsense probably null
belt UTSW 15 94345990 missense probably damaging 1.00
buckeye UTSW 15 94341087 missense probably damaging 1.00
jack_white UTSW 15 unclassified
Meowth UTSW 15 94331458 missense probably damaging 1.00
nidoking UTSW 15 94403445 missense probably damaging 1.00
pikachu UTSW 15 94345990 missense probably damaging 1.00
poliwag UTSW 15 94394622 nonsense probably null
splotch2 UTSW 15 94335561 splice acceptor site
wash UTSW 15 94347670 nonsense probably null
whitebelly UTSW 15 unclassified
whopper UTSW 15 94347810 missense
R0483:Adamts20 UTSW 15 94353571 missense probably benign 0.00
R0514:Adamts20 UTSW 15 94270376 missense probably damaging 1.00
R0568:Adamts20 UTSW 15 94291713 splice site probably benign
R0730:Adamts20 UTSW 15 94347690 missense probably benign 0.00
R0973:Adamts20 UTSW 15 94286371 missense probably benign 0.00
R1339:Adamts20 UTSW 15 94322896 missense probably benign 0.19
R1721:Adamts20 UTSW 15 94338459 missense probably benign 0.44
R1809:Adamts20 UTSW 15 94341087 missense probably damaging 1.00
R1832:Adamts20 UTSW 15 94286344 missense probably benign 0.00
R1846:Adamts20 UTSW 15 94345990 missense probably damaging 1.00
R1867:Adamts20 UTSW 15 94338459 missense probably benign 0.44
R1875:Adamts20 UTSW 15 94331396 missense probably benign 0.01
R1930:Adamts20 UTSW 15 94404010 missense probably benign 0.03
R1931:Adamts20 UTSW 15 94404010 missense probably benign 0.03
R1932:Adamts20 UTSW 15 94404010 missense probably benign 0.03
R2001:Adamts20 UTSW 15 94347718 missense possibly damaging 0.96
R2116:Adamts20 UTSW 15 94355362 missense probably damaging 1.00
R2162:Adamts20 UTSW 15 94331458 missense probably damaging 1.00
R2350:Adamts20 UTSW 15 94283916 missense probably damaging 1.00
R2887:Adamts20 UTSW 15 94330578 missense probably benign 0.00
R2889:Adamts20 UTSW 15 94330578 missense probably benign 0.00
R2890:Adamts20 UTSW 15 94330578 missense probably benign 0.00
R3109:Adamts20 UTSW 15 94345904 splice site probably benign
R3719:Adamts20 UTSW 15 94361838 missense probably damaging 0.99
R3832:Adamts20 UTSW 15 94331458 missense probably damaging 1.00
R3901:Adamts20 UTSW 15 94328845 missense possibly damaging 0.81
R4398:Adamts20 UTSW 15 94333695 missense possibly damaging 0.93
R4402:Adamts20 UTSW 15 94379946 missense probably benign
R4431:Adamts20 UTSW 15 94344043 missense probably damaging 1.00
R4479:Adamts20 UTSW 15 94403445 missense probably damaging 1.00
R4482:Adamts20 UTSW 15 94345920 missense probably damaging 1.00
R4503:Adamts20 UTSW 15 94379750 missense probably damaging 0.99
R4671:Adamts20 UTSW 15 94403325 missense possibly damaging 0.48
R4700:Adamts20 UTSW 15 94394622 nonsense probably null
R4707:Adamts20 UTSW 15 94333647 missense possibly damaging 0.53
R4725:Adamts20 UTSW 15 94351762 missense probably damaging 0.99
R4771:Adamts20 UTSW 15 94351635 splice site probably null
R4829:Adamts20 UTSW 15 94326396 missense probably benign 0.01
R4937:Adamts20 UTSW 15 94379775 missense probably benign
R4960:Adamts20 UTSW 15 94379774 missense probably benign
R5270:Adamts20 UTSW 15 94282519 missense probably benign 0.00
R5388:Adamts20 UTSW 15 94345778 missense possibly damaging 0.81
R5410:Adamts20 UTSW 15 94281957 missense possibly damaging 0.94
R5453:Adamts20 UTSW 15 94326088 missense possibly damaging 0.69
R5611:Adamts20 UTSW 15 94273280 missense possibly damaging 0.65
R5687:Adamts20 UTSW 15 94325971 missense probably benign 0.36
R5758:Adamts20 UTSW 15 94394650 missense probably benign 0.00
R5801:Adamts20 UTSW 15 94347670 nonsense probably null
R5834:Adamts20 UTSW 15 94353584 missense probably damaging 0.99
R5993:Adamts20 UTSW 15 94338723 missense probably damaging 0.99
R5997:Adamts20 UTSW 15 94379747 missense probably damaging 1.00
R6044:Adamts20 UTSW 15 94282483 missense probably damaging 1.00
R6058:Adamts20 UTSW 15 94330047 nonsense probably null
R6217:Adamts20 UTSW 15 94338715 missense probably benign 0.00
R6283:Adamts20 UTSW 15 94351721 missense probably benign
R6354:Adamts20 UTSW 15 94347810 missense probably damaging 1.00
R6415:Adamts20 UTSW 15 94324659 critical splice donor site probably null
R6419:Adamts20 UTSW 15 94333675 missense possibly damaging 0.84
R6476:Adamts20 UTSW 15 94361810 missense probably benign 0.22
R6485:Adamts20 UTSW 15 94343971 missense probably benign 0.17
R6517:Adamts20 UTSW 15 94283104 intron probably null
R6675:Adamts20 UTSW 15 94331316 critical splice donor site probably null
R6863:Adamts20 UTSW 15 94379746 nonsense probably null
Mode of Inheritance Autosomal Recessive
Local Stock Embryos
Repository

none

Last Updated 2018-10-05 4:16 PM by Diantha La Vine
Record Created unknown
Record Posted 2009-02-19
Phenotypic Description

The panda mutation was discovered in ENU-mutagenized mice and causes a splotch2-like appearance with a white spot on the belly that extends across the back to form a belt (Figure 1). 

Nature of Mutation
The panda mutation was mapped to Chromosome 15, and corresponds to a C to A transversion at position 1866 of the Adamts20 transcript, in exon 11 of 39 total exons.
 
1850 CCCTACAGCTCGTGTTCAAGGACATGCGGAGGT
561  -P--Y--S--S--C--S--R--T--C--G--G-
 
The mutated nucleotide is indicated in red lettering, and creates a premature stop codon at codon 566 (normally a serine) deleting 1340 amino acids from the C-terminus of the protein.
Protein Prediction
Figure 1. Domain structure of ADAMTS20. The panda mutation replaces a serine with a stop codon at amino acid 566 of the ADAMTS20 protein. SP, Signal peptide; MP, Metalloprotease; DL, Disintegrin-like; CRR, Cystein-rich region. TSP-type domains are yellow and numbered. Other mutations found in ADAMTS20 are noted in red. Click on each mututation for more specific information.
The panda mutation results in protein truncation in the first thrombospondin type I (TSP1) repeat of the ADAMTS-20 metalloprotease.  This truncation occurs after the metalloprotease domain, but removes most of the protein (Figure 2).  It is unknown whether normal levels of the altered protein exist in panda mice.
 
Please see the record for splotch2 for more information about Adamts20.
Primers Primers cannot be located by automatic search.
Genotyping
Panda genotyping is performed by amplifying the region containing the mutation using PCR, followed by sequencing of the amplified region to detect the single nucleotide change. This protocol has not been tested.
 
Primers for PCR amplification
Panda (F): 5’- TGTCCCCAGGCCCCAATTATATAGATG -3’
Panda (R): 5’- ACGAGAGGAAGATCTCGGCTGTTC -3’
 
PCR program (use SIGMA JumpStart REDTaq)
1) 94°C             2:00
2) 94°C             0:30
3) 56°C             0:30
4) 72°C             1:00
5) repeat steps (2-4) 29X
6) 72°C             7:00
7) 4°C               ∞
 
Primers for sequencing
Panda_seq (F1): 5’- ACAAGCCATTCCTGTGTGTG -3’
Panda_seq (F2): 5’- CCCAGCAGAGAGAGACTATTTATTG -3’
Panda_seq (R2): 5'- GCTGTTCCATTGGAAGCAC
 
The following sequence of 1455 nucleotides (from Genbank genomic region NC_000081 for linear DNA sequence of Adamts20) is amplified:
 
55441        tgt ccccaggccc caattatata gatgttttca ttgcatatat tttctttctt
55501 aaactttaat agaatgtaat tgaatgtgat aaatggcttt ctccaatcta cgtttcatct
55561 ataagtgaaa cacactgtgg tatagtttga agtgatgtag tgtgacaccc acagctgtat
55621 tctttctcgg gattgttttc attgtttgct gtctttgctg ggtccctgtg ggttcttaga
55681 ttgttcttcc ctagttctgt aaagaattat atatgaattt tggtggggat tgcactaact
55741 agttagattc gtttttggta acatagccat tctcatgata ttaagtctgc caatccagtg
55801 gcagggacgg tctttccatt gtctactgct gtctgttgtt tacttcctca agatcccggc
55861 agctttcatt gtagggattg ttcatctcct cagttatatt tattcatatg tttggggtgc
55921 gtgtgtgtat gtgtgtgtgt gtgtgtgtgt gtgtggtgtg tacacgtgta tatgcttgat
55981 tttatacaca cacacacaca cacacacaca cacacacata tatatatgta acacacacac
56041 acacatatgt aacatatgta tgtcacacac acatatatgt atgtaacagt tgtgatgacc
56101 actgcattat gaaaaacact gctttggagc tagtatgaat aggataaaca cttttcattt
56161 taatctcaaa tattcttttt tgcctttcca atgagaacct gaatttcaaa agcaaagaga
56221 aagaattccg ttctttttgc atcccaccaa cctcaggtac ccagcagaga gagactattt
56281 attgcaagct ggtattacat gcagcctcac tcctgagttg tccttggctt tagataatct
56341 acagatcttc tcctgcccat cttcacgctg cggttgcagt gaccgtgatg ttttaactac
56401 agagacacaa gccattcctg tgtgtgtgct tcttctgttc ctagcactgc caccgtgggc
56461 tgtgtgtgac cagggacatg gagacccgtc ctgtggatgg cgagtgggga ccatggggac
56521 cctacagctc gtgttcaagg acatgcggag gtggaatcaa gagcacagcc cggctctgtg
56581 accggcccga gtacgtcttc ttattcactg taaattaaga ttgcccccaa gcaccagaaa
56641 gtcaaaatcg cttccgctca cttccggagc tgggggtcag tctgcgagtt tcagagattc
56701 ggaatccgcc tggaagaatt tcatttgtag gttcagactt tttaaaaatc taaatttctc
56761 agttagaacg gaggcagcta ttttcttcat actctgtttt ctgcttcagt gtgagtttaa
56821 gaagtgaaag tgaatggtga gtctgtgatg gcaccttcat tttatcgtgc ttccaatgga
56881 acagccgaga tcttcctctc gt
 
PCR primer binding sites are underlined; sequencing primer binding sites are highlighted in gray; the mutated C is shown in red text.
Science Writers Nora G. Smart
Illustrators Diantha La Vine
AuthorsSungyong Won, Philippe Georgel, Bruce Beutler.
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Edit History
2011-08-03 4:27 PM (current)
2011-01-07 9:29 AM
2010-11-02 1:22 PM
2010-11-02 1:22 PM
2010-02-03 3:49 PM