Mutagenetix > Incidental Mutation

Incidental Mutation 'R0310:Lama5'

25090

Institutional Source

Beutler Lab

Gene Symbol

Lama5

Ensembl Gene

ENSMUSG00000015647

Gene Name

laminin, alpha 5

Synonyms

MMRRC Submission

038520-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0310 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

179818166-179867652 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

G to T at 179823359 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000015791 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000015791]

AlphaFold

no structure available at present

PDB Structure

LAMININ ALPHA5 CHAIN N-TERMINAL FRAGMENT [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000015791

SMART Domains

Protein: ENSMUSP00000015791
Gene: ENSMUSG00000015647

Domain	Start	End	E-Value	Type
signal peptide	1	40	N/A	INTRINSIC
LamNT	44	303	1.06e-132	SMART
EGF_Lam	305	361	4.35e-6	SMART
EGF_Lam	364	431	5.78e-11	SMART
EGF_Lam	434	476	1.32e-5	SMART
EGF_Lam	500	544	8.63e-10	SMART
EGF_Lam	547	590	1.16e-10	SMART
EGF_Lam	593	635	4.63e-10	SMART
EGF_Lam	638	680	6.25e-7	SMART
EGF_Lam	683	726	3.1e-11	SMART
EGF_Lam	730	779	2.99e-4	SMART
EGF_Lam	782	831	4.66e-6	SMART
EGF_Lam	834	878	3.48e-5	SMART
low complexity region	1261	1273	N/A	INTRINSIC
EGF_Lam	1443	1486	7.01e-10	SMART
EGF_like	1489	1530	3.64e-1	SMART
EGF_Lam	1533	1579	8.56e-14	SMART
EGF_Lam	1582	1630	1.86e-14	SMART
LamB	1689	1819	5.86e-61	SMART
EGF_like	1818	1862	2.74e0	SMART
EGF_Lam	1865	1912	3.32e-11	SMART
EGF_Lam	1915	1968	1.61e-9	SMART
EGF_Lam	1971	2022	6.39e-13	SMART
EGF_Lam	2025	2069	1.94e-12	SMART
EGF_Lam	2072	2116	1.35e-11	SMART
EGF_like	2103	2145	3.1e1	SMART
EGF_Lam	2119	2166	1.18e-2	SMART
Pfam:Laminin_I	2189	2453	1.7e-65	PFAM
low complexity region	2532	2548	N/A	INTRINSIC
low complexity region	2557	2569	N/A	INTRINSIC
low complexity region	2632	2641	N/A	INTRINSIC
low complexity region	2663	2676	N/A	INTRINSIC
LamG	2760	2912	3.97e-8	SMART
LamG	2966	3103	1.78e-10	SMART
LamG	3149	3274	1.11e-20	SMART
LamG	3359	3497	4.05e-23	SMART
LamG	3539	3670	3e-26	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123906

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130796

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000149812

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185089

Coding Region Coverage

1x: 98.6%
3x: 97.3%
10x: 93.3%
20x: 82.5%

Validation Efficiency

99% (113/114)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes one of the vertebrate laminin alpha chains. Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. The protein encoded by this gene is the alpha-5 subunit of of laminin-10 (laminin-511), laminin-11 (laminin-521) and laminin-15 (laminin-523). [provided by RefSeq, Jun 2013]
PHENOTYPE: Homozygotes for a targeted null mutation exhibit disrupted basal laminae leading to exencephaly, syndactyly, placentopathy, kidney defects, abnormal lobar septation with absence of a visceral pleural membrane, and lethality in late gestation. [provided by MGI curators]

Allele List at MGI

All alleles(49) : Targeted(5) Gene trapped(44)

Other mutations in this stock

Total: 109 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930550C14Rik	A	C	9: 53,336,971 (GRCm39)	T92P	probably damaging	Het
Abca13	T	C	11: 9,243,810 (GRCm39)	V1891A	probably benign	Het
Abcc1	T	A	16: 14,228,791 (GRCm39)	I346N	probably damaging	Het
Afdn	G	T	17: 14,105,770 (GRCm39)		probably null	Het
Ahrr	G	A	13: 74,431,143 (GRCm39)		probably benign	Het
Akap13	A	G	7: 75,264,678 (GRCm39)	D507G	probably damaging	Het
Akap8	A	T	17: 32,535,234 (GRCm39)	M260K	possibly damaging	Het
Akr1b8	T	C	6: 34,342,194 (GRCm39)	V265A	probably benign	Het
Alpk3	C	G	7: 80,728,358 (GRCm39)	P496R	possibly damaging	Het
Ankrd13b	A	G	11: 77,363,571 (GRCm39)	V249A	possibly damaging	Het
Arid4a	T	C	12: 71,122,604 (GRCm39)	V995A	probably benign	Het
Ascc3	G	T	10: 50,625,022 (GRCm39)	V1637L	probably benign	Het
Atad2	G	A	15: 57,977,653 (GRCm39)	A499V	probably damaging	Het
Barhl2	G	T	5: 106,605,253 (GRCm39)	A152E	possibly damaging	Het
Bbs12	T	A	3: 37,375,194 (GRCm39)	D547E	probably damaging	Het
Btaf1	A	G	19: 36,981,934 (GRCm39)	M1655V	probably damaging	Het
Ccdc50	T	A	16: 27,225,408 (GRCm39)	H40Q	probably damaging	Het
Ccr9	A	T	9: 123,603,617 (GRCm39)		probably benign	Het
Cdh15	A	G	8: 123,592,175 (GRCm39)	D654G	probably damaging	Het
Cebpz	T	C	17: 79,233,553 (GRCm39)	D758G	probably damaging	Het
Cgn	C	T	3: 94,672,960 (GRCm39)	R906K	possibly damaging	Het
Chil3	T	A	3: 106,067,839 (GRCm39)	M109L	possibly damaging	Het
Cntnap4	A	T	8: 113,569,148 (GRCm39)		probably null	Het
Cyp4f18	C	T	8: 72,754,856 (GRCm39)		probably benign	Het
Daam2	A	G	17: 49,770,952 (GRCm39)		probably null	Het
Ddost	T	A	4: 138,037,922 (GRCm39)	H220Q	probably benign	Het
Dennd2a	C	T	6: 39,441,135 (GRCm39)		probably benign	Het
Depdc1b	G	A	13: 108,510,375 (GRCm39)	V296I	possibly damaging	Het
Dnaaf4	A	T	9: 72,879,618 (GRCm39)	D386V	probably damaging	Het
Dnah5	A	T	15: 28,299,256 (GRCm39)	R1539S	probably benign	Het
Dusp22	T	C	13: 30,889,641 (GRCm39)	I74T	probably damaging	Het
Epc1	A	G	18: 6,440,202 (GRCm39)		probably benign	Het
Epha7	A	G	4: 28,961,301 (GRCm39)	I845V	probably benign	Het
Fanci	C	T	7: 79,057,165 (GRCm39)		probably benign	Het
Fbn1	T	A	2: 125,205,564 (GRCm39)	E1104V	probably damaging	Het
Fbxo8	T	A	8: 57,043,132 (GRCm39)	F205L	probably damaging	Het
Fcgbpl1	G	T	7: 27,841,699 (GRCm39)	V545L	probably benign	Het
Fetub	T	C	16: 22,748,506 (GRCm39)		probably benign	Het
Frs3	T	C	17: 48,014,747 (GRCm39)	V480A	probably benign	Het
Gne	C	A	4: 44,060,157 (GRCm39)	E79*	probably null	Het
Hrc	T	A	7: 44,985,921 (GRCm39)	H357Q	probably benign	Het
Idh1	T	C	1: 65,201,079 (GRCm39)	M291V	probably damaging	Het
Il22b	T	A	10: 118,129,090 (GRCm39)	H133L	probably benign	Het
Ino80b	T	C	6: 83,101,072 (GRCm39)	E165G	probably damaging	Het
Inppl1	A	T	7: 101,477,706 (GRCm39)		probably benign	Het
Ints15	G	T	5: 143,293,643 (GRCm39)	T278K	probably benign	Het
Ip6k2	T	C	9: 108,676,432 (GRCm39)		probably benign	Het
Itga11	A	T	9: 62,667,628 (GRCm39)	I654F	probably damaging	Het
Jag2	G	T	12: 112,876,997 (GRCm39)		probably benign	Het
Katna1	G	T	10: 7,619,513 (GRCm39)		probably benign	Het
Kcnh4	G	T	11: 100,636,995 (GRCm39)	S707Y	probably benign	Het
Kcnn2	T	G	18: 45,693,585 (GRCm39)	L387R	probably damaging	Het
Khnyn	A	G	14: 56,125,425 (GRCm39)	T503A	probably damaging	Het
Lmbr1l	A	T	15: 98,806,654 (GRCm39)		probably benign	Het
Mast4	A	T	13: 102,890,669 (GRCm39)	S870T	possibly damaging	Het
Med1	A	G	11: 98,058,400 (GRCm39)	Y266H	probably benign	Het
Med13	A	T	11: 86,236,829 (GRCm39)	N109K	probably benign	Het
Mgam	T	C	6: 40,737,969 (GRCm39)		probably benign	Het
Mmp8	A	G	9: 7,561,455 (GRCm39)	Q153R	probably benign	Het
Mpeg1	C	A	19: 12,439,055 (GRCm39)	T171N	probably benign	Het
Mtus2	T	C	5: 148,043,829 (GRCm39)	S806P	probably benign	Het
Naip2	T	A	13: 100,285,350 (GRCm39)	E1226V	probably damaging	Het
Naip6	A	G	13: 100,444,721 (GRCm39)	F246L	possibly damaging	Het
Nav3	T	C	10: 109,602,989 (GRCm39)	I1187V	possibly damaging	Het
Nbeal1	T	C	1: 60,344,529 (GRCm39)		probably benign	Het
Nbeal2	C	A	9: 110,467,231 (GRCm39)	V653L	probably damaging	Het
Ndufa9	G	T	6: 126,804,495 (GRCm39)		probably benign	Het
Nlrp5	G	T	7: 23,129,582 (GRCm39)	C883F	probably damaging	Het
Nr0b2	C	A	4: 133,283,303 (GRCm39)		probably null	Het
Or1m1	T	A	9: 18,666,629 (GRCm39)	M101L	possibly damaging	Het
Or6c209	T	A	10: 129,483,600 (GRCm39)	V201E	probably damaging	Het
Or6c69c	G	A	10: 129,910,692 (GRCm39)	V138I	probably benign	Het
Or8b101	T	A	9: 38,020,782 (GRCm39)	S267T	possibly damaging	Het
Pkhd1	A	T	1: 20,620,046 (GRCm39)		probably null	Het
Pkhd1l1	A	G	15: 44,386,134 (GRCm39)		probably benign	Het
Ppm1l	A	G	3: 69,456,794 (GRCm39)	K237R	probably benign	Het
Ppp1r18	T	C	17: 36,184,603 (GRCm39)		probably benign	Het
Ptpn3	A	T	4: 57,204,958 (GRCm39)	D734E	probably benign	Het
Pxdn	T	C	12: 30,065,528 (GRCm39)	C1283R	probably damaging	Het
Rbm12	A	G	2: 155,937,644 (GRCm39)		probably benign	Het
Rttn	A	T	18: 89,027,584 (GRCm39)		probably benign	Het
Sgsm1	G	T	5: 113,411,571 (GRCm39)	H431Q	probably benign	Het
Siah3	A	G	14: 75,763,367 (GRCm39)	N206S	possibly damaging	Het
Slc22a15	G	T	3: 101,767,827 (GRCm39)	D521E	probably benign	Het
Sprr2k	A	T	3: 92,340,770 (GRCm39)		probably benign	Het
Stab2	G	A	10: 86,803,477 (GRCm39)		probably benign	Het
Sval3	T	A	6: 41,945,120 (GRCm39)	L16Q	probably damaging	Het
Sycp2	C	G	2: 178,023,648 (GRCm39)	S456T	probably benign	Het
Tk1	T	C	11: 117,707,921 (GRCm39)		probably benign	Het
Tlk2	C	A	11: 105,145,799 (GRCm39)	A335E	probably benign	Het
Tmtc1	T	C	6: 148,151,079 (GRCm39)	K659E	probably benign	Het
Tnk2	C	T	16: 32,499,408 (GRCm39)	P907L	probably benign	Het
Trim14	C	A	4: 46,522,043 (GRCm39)	K211N	probably damaging	Het
Trim15	A	C	17: 37,177,878 (GRCm39)	L39R	probably damaging	Het
Tspan15	T	A	10: 62,023,872 (GRCm39)	T269S	probably benign	Het
Ttc7	T	A	17: 87,669,292 (GRCm39)	D646E	probably benign	Het
Ttll6	A	T	11: 96,038,382 (GRCm39)	Q410L	probably benign	Het
Unc79	A	G	12: 103,027,666 (GRCm39)	Q419R	probably damaging	Het
Vcam1	A	T	3: 115,908,065 (GRCm39)	Y666N	possibly damaging	Het
Vmn1r10	A	T	6: 57,090,486 (GRCm39)	Y26F	probably damaging	Het
Vmn1r80	A	G	7: 11,927,775 (GRCm39)	N295S	probably benign	Het
Vmn2r114	A	T	17: 23,509,917 (GRCm39)	H854Q	probably benign	Het
Vmn2r2	A	T	3: 64,042,039 (GRCm39)	D225E	probably damaging	Het
Vmn2r4	A	T	3: 64,296,855 (GRCm39)	Y643*	probably null	Het
Vmn2r52	T	A	7: 9,893,393 (GRCm39)	Y582F	probably damaging	Het
Vmn2r60	G	T	7: 41,844,564 (GRCm39)	L642F	possibly damaging	Het
Zbtb20	T	C	16: 43,430,109 (GRCm39)	S207P	probably damaging	Het
Zic2	CCCACCACCACCATCACCACCACCACC	CCCACCATCACCACCACCACC	14: 122,713,776 (GRCm39)		probably benign	Het
Zkscan7	G	T	9: 122,717,958 (GRCm39)	E118*	probably null	Het

Other mutations in Lama5

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01067:Lama5	APN	2	179,818,336 (GRCm39)	unclassified	probably benign
IGL01370:Lama5	APN	2	179,839,193 (GRCm39)	missense	possibly damaging	0.87
IGL01474:Lama5	APN	2	179,838,363 (GRCm39)	missense	probably damaging	1.00
IGL01614:Lama5	APN	2	179,822,657 (GRCm39)	missense	probably damaging	1.00
IGL01941:Lama5	APN	2	179,834,185 (GRCm39)	missense	possibly damaging	0.71
IGL01953:Lama5	APN	2	179,832,497 (GRCm39)	missense	probably damaging	0.97
IGL02093:Lama5	APN	2	179,830,380 (GRCm39)	missense	probably damaging	1.00
IGL02197:Lama5	APN	2	179,849,012 (GRCm39)	missense	possibly damaging	0.82
IGL02308:Lama5	APN	2	179,832,120 (GRCm39)	splice site	probably benign
IGL02314:Lama5	APN	2	179,836,275 (GRCm39)	splice site	probably benign
IGL02317:Lama5	APN	2	179,833,112 (GRCm39)	missense	probably damaging	1.00
IGL02354:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02361:Lama5	APN	2	179,835,677 (GRCm39)	nonsense	probably null
IGL02557:Lama5	APN	2	179,832,725 (GRCm39)	nonsense	probably null
IGL03026:Lama5	APN	2	179,837,760 (GRCm39)	missense	probably benign	0.34
IGL03160:Lama5	APN	2	179,822,128 (GRCm39)	missense	probably damaging	1.00
IGL03238:Lama5	APN	2	179,830,367 (GRCm39)	missense	probably benign
IGL03390:Lama5	APN	2	179,849,011 (GRCm39)	missense	probably damaging	1.00
blancmange	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
cupcake	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
layercake	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
poundcake	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
Salty	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
PIT4378001:Lama5	UTSW	2	179,831,238 (GRCm39)	missense	possibly damaging	0.89
R0003:Lama5	UTSW	2	179,819,872 (GRCm39)	splice site	probably null
R0056:Lama5	UTSW	2	179,828,899 (GRCm39)	intron	probably benign
R0147:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0148:Lama5	UTSW	2	179,832,199 (GRCm39)	missense	probably benign
R0326:Lama5	UTSW	2	179,824,219 (GRCm39)	missense	possibly damaging	0.90
R0368:Lama5	UTSW	2	179,823,023 (GRCm39)	nonsense	probably null
R0479:Lama5	UTSW	2	179,826,250 (GRCm39)	missense	probably benign	0.03
R0490:Lama5	UTSW	2	179,821,962 (GRCm39)	missense	possibly damaging	0.90
R0636:Lama5	UTSW	2	179,831,124 (GRCm39)	critical splice donor site	probably null
R0704:Lama5	UTSW	2	179,821,277 (GRCm39)	missense	possibly damaging	0.84
R0733:Lama5	UTSW	2	179,822,511 (GRCm39)	missense	possibly damaging	0.83
R1017:Lama5	UTSW	2	179,837,213 (GRCm39)	missense	probably damaging	1.00
R1078:Lama5	UTSW	2	179,821,557 (GRCm39)	unclassified	probably benign
R1294:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1423:Lama5	UTSW	2	179,837,434 (GRCm39)	missense	probably damaging	1.00
R1438:Lama5	UTSW	2	179,824,593 (GRCm39)	missense	probably benign	0.01
R1447:Lama5	UTSW	2	179,827,671 (GRCm39)	missense	probably damaging	0.99
R1540:Lama5	UTSW	2	179,821,944 (GRCm39)	missense	probably benign
R1601:Lama5	UTSW	2	179,839,538 (GRCm39)	missense	probably damaging	1.00
R1624:Lama5	UTSW	2	179,848,551 (GRCm39)	missense	probably benign	0.02
R1674:Lama5	UTSW	2	179,843,780 (GRCm39)	missense	probably benign	0.00
R1687:Lama5	UTSW	2	179,835,859 (GRCm39)	missense	probably benign	0.00
R1696:Lama5	UTSW	2	179,844,279 (GRCm39)	missense	probably damaging	1.00
R1701:Lama5	UTSW	2	179,863,162 (GRCm39)	missense	probably damaging	1.00
R1778:Lama5	UTSW	2	179,837,274 (GRCm39)	splice site	probably benign
R1936:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1939:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1940:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R1953:Lama5	UTSW	2	179,832,540 (GRCm39)	missense	possibly damaging	0.94
R1966:Lama5	UTSW	2	179,830,145 (GRCm39)	missense	probably damaging	1.00
R2024:Lama5	UTSW	2	179,820,923 (GRCm39)	missense	probably benign	0.00
R2079:Lama5	UTSW	2	179,867,301 (GRCm39)	missense	possibly damaging	0.68
R2115:Lama5	UTSW	2	179,828,678 (GRCm39)	missense	probably damaging	1.00
R2173:Lama5	UTSW	2	179,838,035 (GRCm39)	missense	probably benign	0.00
R2272:Lama5	UTSW	2	179,820,396 (GRCm39)	missense	possibly damaging	0.93
R2357:Lama5	UTSW	2	179,821,890 (GRCm39)	missense	probably benign	0.01
R2860:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2861:Lama5	UTSW	2	179,829,040 (GRCm39)	missense	probably benign	0.00
R2939:Lama5	UTSW	2	179,840,747 (GRCm39)	missense	probably damaging	1.00
R3053:Lama5	UTSW	2	179,824,860 (GRCm39)	missense	probably damaging	0.99
R3430:Lama5	UTSW	2	179,838,110 (GRCm39)	missense	probably benign	0.00
R3752:Lama5	UTSW	2	179,829,015 (GRCm39)	missense	probably damaging	1.00
R3782:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	possibly damaging	0.57
R3901:Lama5	UTSW	2	179,824,144 (GRCm39)	splice site	probably benign
R4248:Lama5	UTSW	2	179,822,220 (GRCm39)	missense	possibly damaging	0.84
R4626:Lama5	UTSW	2	179,826,253 (GRCm39)	missense	probably damaging	0.98
R4638:Lama5	UTSW	2	179,832,206 (GRCm39)	missense	possibly damaging	0.89
R4669:Lama5	UTSW	2	179,822,430 (GRCm39)	missense	probably damaging	1.00
R4673:Lama5	UTSW	2	179,841,059 (GRCm39)	missense	probably damaging	1.00
R4677:Lama5	UTSW	2	179,821,159 (GRCm39)	missense	possibly damaging	0.69
R4701:Lama5	UTSW	2	179,833,489 (GRCm39)	missense	probably damaging	1.00
R4774:Lama5	UTSW	2	179,827,734 (GRCm39)	missense	probably damaging	1.00
R4880:Lama5	UTSW	2	179,818,861 (GRCm39)	unclassified	probably benign
R4923:Lama5	UTSW	2	179,825,942 (GRCm39)	missense	probably benign	0.18
R4960:Lama5	UTSW	2	179,850,045 (GRCm39)	critical splice donor site	probably null
R4983:Lama5	UTSW	2	179,835,242 (GRCm39)	missense	probably benign	0.13
R5061:Lama5	UTSW	2	179,840,579 (GRCm39)	nonsense	probably null
R5080:Lama5	UTSW	2	179,848,993 (GRCm39)	nonsense	probably null
R5135:Lama5	UTSW	2	179,844,013 (GRCm39)	missense	possibly damaging	0.89
R5206:Lama5	UTSW	2	179,833,097 (GRCm39)	missense	probably damaging	1.00
R5296:Lama5	UTSW	2	179,835,594 (GRCm39)	missense	probably damaging	1.00
R5319:Lama5	UTSW	2	179,822,911 (GRCm39)	missense	probably damaging	1.00
R5355:Lama5	UTSW	2	179,823,444 (GRCm39)	missense	possibly damaging	0.84
R5388:Lama5	UTSW	2	179,832,539 (GRCm39)	missense	possibly damaging	0.83
R5528:Lama5	UTSW	2	179,836,356 (GRCm39)	missense	probably benign	0.21
R5536:Lama5	UTSW	2	179,831,142 (GRCm39)	missense	probably damaging	0.99
R5658:Lama5	UTSW	2	179,850,069 (GRCm39)	nonsense	probably null
R5823:Lama5	UTSW	2	179,834,285 (GRCm39)	missense	probably benign	0.04
R5885:Lama5	UTSW	2	179,843,624 (GRCm39)	missense	probably damaging	1.00
R5889:Lama5	UTSW	2	179,835,467 (GRCm39)	intron	probably benign
R5912:Lama5	UTSW	2	179,837,268 (GRCm39)	missense	probably damaging	1.00
R5955:Lama5	UTSW	2	179,839,267 (GRCm39)	missense	probably damaging	1.00
R6015:Lama5	UTSW	2	179,827,185 (GRCm39)	missense	probably benign	0.36
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6037:Lama5	UTSW	2	179,848,806 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,827,752 (GRCm39)	missense	probably damaging	1.00
R6191:Lama5	UTSW	2	179,822,404 (GRCm39)	missense	probably damaging	0.98
R6359:Lama5	UTSW	2	179,837,775 (GRCm39)	missense	probably benign	0.01
R6385:Lama5	UTSW	2	179,838,326 (GRCm39)	missense	probably damaging	1.00
R6406:Lama5	UTSW	2	179,839,257 (GRCm39)	nonsense	probably null
R6552:Lama5	UTSW	2	179,822,947 (GRCm39)	missense	probably damaging	0.98
R6632:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6633:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6645:Lama5	UTSW	2	179,821,463 (GRCm39)	missense	probably damaging	1.00
R6731:Lama5	UTSW	2	179,830,367 (GRCm39)	missense	probably benign	0.09
R6744:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6798:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6799:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6801:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6851:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6869:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6881:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6882:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R6884:Lama5	UTSW	2	179,833,455 (GRCm39)	missense	probably damaging	1.00
R7022:Lama5	UTSW	2	179,822,524 (GRCm39)	missense	probably damaging	1.00
R7204:Lama5	UTSW	2	179,843,970 (GRCm39)	missense	probably damaging	1.00
R7207:Lama5	UTSW	2	179,848,877 (GRCm39)	missense	probably damaging	0.98
R7282:Lama5	UTSW	2	179,843,588 (GRCm39)	missense	probably damaging	1.00
R7367:Lama5	UTSW	2	179,834,751 (GRCm39)	missense	probably benign	0.01
R7410:Lama5	UTSW	2	179,844,183 (GRCm39)	critical splice donor site	probably null
R7699:Lama5	UTSW	2	179,822,654 (GRCm39)	missense	probably damaging	1.00
R7849:Lama5	UTSW	2	179,843,605 (GRCm39)	missense	probably damaging	1.00
R7909:Lama5	UTSW	2	179,834,069 (GRCm39)	missense	possibly damaging	0.95
R7948:Lama5	UTSW	2	179,843,994 (GRCm39)	missense	probably damaging	1.00
R8153:Lama5	UTSW	2	179,829,724 (GRCm39)	missense	probably benign	0.37
R8317:Lama5	UTSW	2	179,848,784 (GRCm39)	missense	probably damaging	1.00
R8351:Lama5	UTSW	2	179,837,401 (GRCm39)	missense	probably damaging	1.00
R8370:Lama5	UTSW	2	179,843,280 (GRCm39)	missense	possibly damaging	0.80
R8398:Lama5	UTSW	2	179,838,827 (GRCm39)	critical splice donor site	probably null
R8401:Lama5	UTSW	2	179,840,580 (GRCm39)	missense	probably damaging	1.00
R8404:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8502:Lama5	UTSW	2	179,837,015 (GRCm39)	missense	probably damaging	1.00
R8694:Lama5	UTSW	2	179,822,677 (GRCm39)	missense	probably damaging	0.98
R8705:Lama5	UTSW	2	179,820,354 (GRCm39)	missense	probably damaging	1.00
R8732:Lama5	UTSW	2	179,828,481 (GRCm39)	missense	probably damaging	1.00
R8755:Lama5	UTSW	2	179,832,714 (GRCm39)	missense	probably benign	0.00
R8786:Lama5	UTSW	2	179,838,100 (GRCm39)	missense	probably damaging	1.00
R8926:Lama5	UTSW	2	179,835,783 (GRCm39)	missense	probably benign	0.08
R8928:Lama5	UTSW	2	179,843,832 (GRCm39)	missense	probably damaging	1.00
R8953:Lama5	UTSW	2	179,835,313 (GRCm39)	missense	probably damaging	0.99
R8958:Lama5	UTSW	2	179,835,592 (GRCm39)	missense	probably benign
R9002:Lama5	UTSW	2	179,838,311 (GRCm39)	missense	probably damaging	1.00
R9081:Lama5	UTSW	2	179,833,930 (GRCm39)	nonsense	probably null
R9165:Lama5	UTSW	2	179,821,286 (GRCm39)	missense	probably damaging	0.99
R9233:Lama5	UTSW	2	179,840,502 (GRCm39)	nonsense	probably null
R9264:Lama5	UTSW	2	179,838,271 (GRCm39)	splice site	probably benign
R9311:Lama5	UTSW	2	179,838,275 (GRCm39)	critical splice donor site	probably null
R9443:Lama5	UTSW	2	179,843,522 (GRCm39)	missense	probably benign	0.00
R9488:Lama5	UTSW	2	179,823,234 (GRCm39)	missense	possibly damaging	0.95
R9674:Lama5	UTSW	2	179,840,267 (GRCm39)	critical splice donor site	probably null
R9684:Lama5	UTSW	2	179,849,038 (GRCm39)	missense	probably damaging	1.00
R9749:Lama5	UTSW	2	179,825,433 (GRCm39)	missense	probably benign	0.00
RF020:Lama5	UTSW	2	179,837,971 (GRCm39)	missense	probably benign
X0065:Lama5	UTSW	2	179,823,524 (GRCm39)	missense	probably benign	0.26
Z1177:Lama5	UTSW	2	179,832,507 (GRCm39)	missense	possibly damaging	0.95
Z1177:Lama5	UTSW	2	179,831,212 (GRCm39)	missense	probably damaging	1.00
Z1177:Lama5	UTSW	2	179,825,423 (GRCm39)	missense	probably benign	0.03
Z1177:Lama5	UTSW	2	179,840,603 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGACCTGTGAGTGTAGCACGAGAG -3'
(R):5'- CATGAAATTGGCTGCTGCTGGG -3'

Sequencing Primer
(F):5'- CACGAGAGGGTTTACTACCTG -3'
(R):5'- TCCATCTGTTACAGGCGAGAC -3'

Posted On

2013-04-16