Mutagenetix > Incidental Mutation

Incidental Mutation 'R4819:Pcnx2'

370056

Institutional Source

Beutler Lab

Gene Symbol

Pcnx2

Ensembl Gene

ENSMUSG00000060212

Gene Name

pecanex homolog 2

Synonyms

Pcnxl2, E330039K12Rik

MMRRC Submission

042000-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4819 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

126478247-126625056 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 126581969 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 922 (F922L)

Ref Sequence

ENSEMBL: ENSMUSP00000119965 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047239] [ENSMUST00000131127]

AlphaFold

Q5DU28

Predicted Effect

probably benign
Transcript: ENSMUST00000047239
AA Change: F922L

PolyPhen 2 Score 0.037 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000042294
Gene: ENSMUSG00000060212
AA Change: F922L

Domain	Start	End	E-Value	Type
transmembrane domain	36	53	N/A	INTRINSIC
transmembrane domain	60	82	N/A	INTRINSIC
low complexity region	94	104	N/A	INTRINSIC
low complexity region	391	415	N/A	INTRINSIC
low complexity region	457	476	N/A	INTRINSIC
low complexity region	727	742	N/A	INTRINSIC
low complexity region	806	817	N/A	INTRINSIC
transmembrane domain	823	842	N/A	INTRINSIC
transmembrane domain	849	866	N/A	INTRINSIC
transmembrane domain	881	902	N/A	INTRINSIC
transmembrane domain	934	956	N/A	INTRINSIC
transmembrane domain	976	998	N/A	INTRINSIC
transmembrane domain	1011	1030	N/A	INTRINSIC
transmembrane domain	1080	1102	N/A	INTRINSIC
transmembrane domain	1104	1126	N/A	INTRINSIC
Pfam:Pecanex_C	1603	1828	3.5e-113	PFAM
low complexity region	1864	1889	N/A	INTRINSIC
low complexity region	1968	1981	N/A	INTRINSIC
low complexity region	2004	2019	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000131127
AA Change: F922L

PolyPhen 2 Score 0.037 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000119965
Gene: ENSMUSG00000060212
AA Change: F922L

Domain	Start	End	E-Value	Type
transmembrane domain	36	53	N/A	INTRINSIC
transmembrane domain	60	82	N/A	INTRINSIC
low complexity region	94	104	N/A	INTRINSIC
low complexity region	391	415	N/A	INTRINSIC
low complexity region	457	476	N/A	INTRINSIC
low complexity region	727	742	N/A	INTRINSIC
low complexity region	806	817	N/A	INTRINSIC
transmembrane domain	823	842	N/A	INTRINSIC
transmembrane domain	849	866	N/A	INTRINSIC
transmembrane domain	934	956	N/A	INTRINSIC
transmembrane domain	968	990	N/A	INTRINSIC
transmembrane domain	1010	1029	N/A	INTRINSIC

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.5%
20x: 93.3%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene contains coding mononucleotide repeats that are associated with tumors of high mcrosatellite instability (MSI-H). Defects in this gene are involved in the tumorigenesis of MSI-H colorectal carcinomas. [provided by RefSeq, Jun 2016]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,240,421 (GRCm39)	N761K	possibly damaging	Het
Adgrf3	T	A	5: 30,403,442 (GRCm39)	L444F	possibly damaging	Het
Akap8	G	A	17: 32,531,279 (GRCm39)	R378W	probably damaging	Het
Amotl2	C	T	9: 102,607,270 (GRCm39)	R693W	probably damaging	Het
As3mt	G	T	19: 46,695,968 (GRCm39)		probably benign	Het
Atp6v1e2	A	G	17: 87,251,966 (GRCm39)	V144A	probably benign	Het
Bfar	C	T	16: 13,505,331 (GRCm39)	Q114*	probably null	Het
Casd1	C	T	6: 4,621,225 (GRCm39)	A261V	probably damaging	Het
Cd177	A	T	7: 24,451,696 (GRCm39)	I440K	probably damaging	Het
Cfap54	G	T	10: 92,672,339 (GRCm39)	Y2910*	probably null	Het
Csl	A	G	10: 99,593,944 (GRCm39)	F374L	possibly damaging	Het
Dctn1	G	A	6: 83,167,501 (GRCm39)	R275H	probably damaging	Het
Derl3	A	G	10: 75,729,713 (GRCm39)		probably null	Het
Dst	A	G	1: 34,007,916 (GRCm39)	I117V	probably benign	Het
Edc3	T	A	9: 57,655,680 (GRCm39)	C477S	possibly damaging	Het
Efs	T	C	14: 55,154,610 (GRCm39)	E450G	probably damaging	Het
Fcrla	T	A	1: 170,748,508 (GRCm39)	I212F	probably damaging	Het
Fsip2	A	G	2: 82,818,786 (GRCm39)	I4840V	probably benign	Het
Gpam	T	A	19: 55,066,773 (GRCm39)	I581F	probably benign	Het
Greb1l	A	G	18: 10,458,358 (GRCm39)	D45G	probably damaging	Het
Heca	A	G	10: 17,783,820 (GRCm39)	Y478H	probably damaging	Het
Hspa9	C	T	18: 35,072,441 (GRCm39)	M561I	probably damaging	Het
Hyal6	T	A	6: 24,734,965 (GRCm39)	Y299*	probably null	Het
Iho1	A	G	9: 108,283,877 (GRCm39)	V189A	probably benign	Het
Ik	T	A	18: 36,886,310 (GRCm39)		probably null	Het
Khsrp	A	G	17: 57,330,360 (GRCm39)	S582P	possibly damaging	Het
Kif18a	A	G	2: 109,122,471 (GRCm39)	D182G	probably damaging	Het
Krt1c	T	C	15: 101,719,979 (GRCm39)	T564A	unknown	Het
Lig4	A	G	8: 10,021,885 (GRCm39)	S632P	probably benign	Het
Med1	C	T	11: 98,046,258 (GRCm39)		probably benign	Het
Mgat3	T	A	15: 80,096,550 (GRCm39)	I459N	probably damaging	Het
Mkln1	A	T	6: 31,451,421 (GRCm39)	Q454L	probably benign	Het
Mn1	A	G	5: 111,567,803 (GRCm39)	E591G	possibly damaging	Het
Myo5c	T	A	9: 75,199,484 (GRCm39)	L1364Q	probably damaging	Het
Oas1d	T	C	5: 121,053,780 (GRCm39)	V80A	probably damaging	Het
Obscn	A	T	11: 58,929,674 (GRCm39)	D5180E	probably damaging	Het
Pax6	G	A	2: 105,522,622 (GRCm39)		probably null	Het
Pcdh15	A	C	10: 74,160,221 (GRCm39)	N446T	probably damaging	Het
Ptpn4	G	T	1: 119,587,580 (GRCm39)	T921K	probably benign	Het
Selenov	A	G	7: 27,989,746 (GRCm39)		probably null	Het
Tmem100	A	G	11: 89,926,271 (GRCm39)	T33A	probably benign	Het
Tmem59	C	T	4: 107,044,878 (GRCm39)	Q66*	probably null	Het
Trav21-dv12	T	A	14: 54,114,070 (GRCm39)	Y63*	probably null	Het
Trim66	G	T	7: 109,056,793 (GRCm39)	H1121Q	probably damaging	Het
Trim80	A	G	11: 115,338,769 (GRCm39)	Y533C	probably damaging	Het
Ttc17	G	A	2: 94,194,955 (GRCm39)	P520L	probably damaging	Het
Ttn	A	T	2: 76,622,093 (GRCm39)	V15483E	probably damaging	Het
Vinac1	G	T	2: 128,882,721 (GRCm39)	N98K	probably damaging	Het
Zdhhc1	CGGGGG	CGGGGGG	8: 106,210,376 (GRCm39)		probably null	Het
Zfhx4	C	A	3: 5,468,974 (GRCm39)	T3069K	probably benign	Het
Zfp281	A	G	1: 136,553,448 (GRCm39)	H142R	probably benign	Het
Zfp462	G	T	4: 55,060,044 (GRCm39)	R1190L	probably damaging	Het
Zfp935	T	A	13: 62,602,231 (GRCm39)	H323L	probably damaging	Het

Other mutations in Pcnx2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00898:Pcnx2	APN	8	126,614,324 (GRCm39)	missense	probably damaging	1.00
IGL00900:Pcnx2	APN	8	126,589,975 (GRCm39)	splice site	probably benign
IGL01134:Pcnx2	APN	8	126,589,889 (GRCm39)	missense	probably benign
IGL01370:Pcnx2	APN	8	126,528,222 (GRCm39)	missense	probably damaging	0.96
IGL01452:Pcnx2	APN	8	126,564,771 (GRCm39)	missense	probably damaging	1.00
IGL01477:Pcnx2	APN	8	126,512,044 (GRCm39)	missense	probably damaging	1.00
IGL01610:Pcnx2	APN	8	126,566,372 (GRCm39)	missense	possibly damaging	0.67
IGL01640:Pcnx2	APN	8	126,528,297 (GRCm39)	missense	probably benign	0.14
IGL01645:Pcnx2	APN	8	126,614,656 (GRCm39)	missense	probably damaging	1.00
IGL01876:Pcnx2	APN	8	126,592,770 (GRCm39)	missense	probably benign	0.31
IGL01933:Pcnx2	APN	8	126,488,393 (GRCm39)	missense	probably damaging	1.00
IGL02208:Pcnx2	APN	8	126,478,894 (GRCm39)	missense	probably benign	0.30
IGL02573:Pcnx2	APN	8	126,582,012 (GRCm39)	missense	probably benign	0.34
IGL02810:Pcnx2	APN	8	126,613,942 (GRCm39)	missense	probably benign	0.03
IGL02859:Pcnx2	APN	8	126,589,912 (GRCm39)	missense	probably damaging	1.00
IGL02879:Pcnx2	APN	8	126,498,796 (GRCm39)	missense	probably damaging	1.00
IGL03202:Pcnx2	APN	8	126,498,783 (GRCm39)	missense	probably damaging	0.98
IGL03259:Pcnx2	APN	8	126,480,388 (GRCm39)	missense	probably benign	0.19
IGL03395:Pcnx2	APN	8	126,614,262 (GRCm39)	missense	probably benign	0.00
IGL03410:Pcnx2	APN	8	126,613,779 (GRCm39)	missense	probably damaging	1.00
gallen	UTSW	8	126,617,859 (GRCm39)	missense	probably damaging	1.00
hotzone	UTSW	8	126,617,880 (GRCm39)	missense	probably benign	0.00
R0107:Pcnx2	UTSW	8	126,480,325 (GRCm39)	missense	probably benign	0.29
R0477:Pcnx2	UTSW	8	126,488,306 (GRCm39)	missense	probably damaging	0.99
R0610:Pcnx2	UTSW	8	126,566,426 (GRCm39)	missense	probably damaging	1.00
R0645:Pcnx2	UTSW	8	126,487,459 (GRCm39)	missense	possibly damaging	0.64
R0894:Pcnx2	UTSW	8	126,613,665 (GRCm39)	splice site	probably benign
R1083:Pcnx2	UTSW	8	126,498,843 (GRCm39)	missense	probably damaging	1.00
R1199:Pcnx2	UTSW	8	126,614,053 (GRCm39)	missense	possibly damaging	0.60
R1296:Pcnx2	UTSW	8	126,500,572 (GRCm39)	missense	probably damaging	1.00
R1445:Pcnx2	UTSW	8	126,479,023 (GRCm39)	missense	probably damaging	0.99
R1467:Pcnx2	UTSW	8	126,480,289 (GRCm39)	missense	possibly damaging	0.77
R1467:Pcnx2	UTSW	8	126,480,289 (GRCm39)	missense	possibly damaging	0.77
R1524:Pcnx2	UTSW	8	126,617,880 (GRCm39)	missense	probably benign	0.00
R1537:Pcnx2	UTSW	8	126,604,188 (GRCm39)	missense	possibly damaging	0.94
R1574:Pcnx2	UTSW	8	126,500,669 (GRCm39)	missense	probably damaging	1.00
R1574:Pcnx2	UTSW	8	126,500,669 (GRCm39)	missense	probably damaging	1.00
R1593:Pcnx2	UTSW	8	126,486,012 (GRCm39)	missense	probably benign	0.11
R1598:Pcnx2	UTSW	8	126,498,825 (GRCm39)	missense	probably benign	0.03
R1603:Pcnx2	UTSW	8	126,566,365 (GRCm39)	missense	probably damaging	1.00
R1697:Pcnx2	UTSW	8	126,577,087 (GRCm39)	missense	probably damaging	1.00
R1759:Pcnx2	UTSW	8	126,500,717 (GRCm39)	missense	probably damaging	1.00
R1855:Pcnx2	UTSW	8	126,534,735 (GRCm39)	splice site	probably benign
R1863:Pcnx2	UTSW	8	126,545,525 (GRCm39)	missense	probably damaging	0.98
R1930:Pcnx2	UTSW	8	126,614,453 (GRCm39)	missense	probably benign	0.10
R1967:Pcnx2	UTSW	8	126,542,422 (GRCm39)	missense	possibly damaging	0.51
R1974:Pcnx2	UTSW	8	126,614,110 (GRCm39)	missense	probably benign	0.00
R1998:Pcnx2	UTSW	8	126,613,882 (GRCm39)	missense	probably damaging	1.00
R2034:Pcnx2	UTSW	8	126,545,406 (GRCm39)	critical splice donor site	probably null
R2072:Pcnx2	UTSW	8	126,488,481 (GRCm39)	missense	possibly damaging	0.90
R2096:Pcnx2	UTSW	8	126,485,987 (GRCm39)	missense	probably benign	0.27
R2216:Pcnx2	UTSW	8	126,614,816 (GRCm39)	missense	probably benign	0.00
R2290:Pcnx2	UTSW	8	126,604,334 (GRCm39)	splice site	probably benign
R2373:Pcnx2	UTSW	8	126,480,190 (GRCm39)	missense	probably damaging	1.00
R2484:Pcnx2	UTSW	8	126,617,859 (GRCm39)	missense	probably damaging	1.00
R2849:Pcnx2	UTSW	8	126,487,666 (GRCm39)	missense	probably damaging	1.00
R2891:Pcnx2	UTSW	8	126,617,797 (GRCm39)	missense	probably damaging	1.00
R2892:Pcnx2	UTSW	8	126,617,797 (GRCm39)	missense	probably damaging	1.00
R2970:Pcnx2	UTSW	8	126,528,275 (GRCm39)	missense	probably damaging	1.00
R3013:Pcnx2	UTSW	8	126,614,509 (GRCm39)	missense	probably benign	0.05
R3608:Pcnx2	UTSW	8	126,614,840 (GRCm39)	missense	probably benign
R3876:Pcnx2	UTSW	8	126,614,897 (GRCm39)	missense	probably benign
R4349:Pcnx2	UTSW	8	126,489,590 (GRCm39)	missense	probably damaging	0.98
R4352:Pcnx2	UTSW	8	126,489,590 (GRCm39)	missense	probably damaging	0.98
R4353:Pcnx2	UTSW	8	126,489,590 (GRCm39)	missense	probably damaging	0.98
R4361:Pcnx2	UTSW	8	126,495,037 (GRCm39)	nonsense	probably null
R4735:Pcnx2	UTSW	8	126,554,780 (GRCm39)	critical splice donor site	probably null
R4749:Pcnx2	UTSW	8	126,614,327 (GRCm39)	missense	probably damaging	1.00
R4812:Pcnx2	UTSW	8	126,592,678 (GRCm39)	missense	probably benign	0.00
R4829:Pcnx2	UTSW	8	126,587,797 (GRCm39)	splice site	probably null
R4832:Pcnx2	UTSW	8	126,478,927 (GRCm39)	missense	probably damaging	0.99
R4876:Pcnx2	UTSW	8	126,498,847 (GRCm39)	missense	probably damaging	1.00
R4974:Pcnx2	UTSW	8	126,577,869 (GRCm39)	missense	probably benign	0.00
R5057:Pcnx2	UTSW	8	126,581,930 (GRCm39)	missense	possibly damaging	0.95
R5078:Pcnx2	UTSW	8	126,478,895 (GRCm39)	missense	probably benign
R5114:Pcnx2	UTSW	8	126,564,749 (GRCm39)	missense	possibly damaging	0.89
R5195:Pcnx2	UTSW	8	126,528,288 (GRCm39)	missense	possibly damaging	0.69
R5239:Pcnx2	UTSW	8	126,587,821 (GRCm39)	splice site	probably null
R5348:Pcnx2	UTSW	8	126,545,495 (GRCm39)	missense	probably damaging	1.00
R5398:Pcnx2	UTSW	8	126,614,687 (GRCm39)	missense	possibly damaging	0.63
R5448:Pcnx2	UTSW	8	126,614,888 (GRCm39)	missense	probably benign	0.14
R5534:Pcnx2	UTSW	8	126,564,754 (GRCm39)	missense	possibly damaging	0.65
R5624:Pcnx2	UTSW	8	126,488,262 (GRCm39)	critical splice donor site	probably null
R5629:Pcnx2	UTSW	8	126,624,780 (GRCm39)	missense	probably damaging	1.00
R5630:Pcnx2	UTSW	8	126,587,697 (GRCm39)	missense	probably damaging	0.99
R5782:Pcnx2	UTSW	8	126,480,223 (GRCm39)	missense	probably damaging	1.00
R5877:Pcnx2	UTSW	8	126,480,467 (GRCm39)	missense	probably damaging	0.99
R5879:Pcnx2	UTSW	8	126,500,685 (GRCm39)	missense	probably damaging	1.00
R6114:Pcnx2	UTSW	8	126,500,686 (GRCm39)	missense	probably damaging	1.00
R6152:Pcnx2	UTSW	8	126,480,491 (GRCm39)	missense	probably damaging	0.99
R6154:Pcnx2	UTSW	8	126,489,552 (GRCm39)	missense	probably damaging	1.00
R6283:Pcnx2	UTSW	8	126,604,325 (GRCm39)	missense	probably damaging	0.99
R6500:Pcnx2	UTSW	8	126,480,224 (GRCm39)	missense	probably damaging	1.00
R6629:Pcnx2	UTSW	8	126,617,851 (GRCm39)	missense	probably benign	0.00
R6708:Pcnx2	UTSW	8	126,587,692 (GRCm39)	critical splice donor site	probably null
R6736:Pcnx2	UTSW	8	126,479,056 (GRCm39)	splice site	probably null
R6748:Pcnx2	UTSW	8	126,577,074 (GRCm39)	missense	probably damaging	1.00
R6788:Pcnx2	UTSW	8	126,498,839 (GRCm39)	missense	probably damaging	1.00
R6849:Pcnx2	UTSW	8	126,587,949 (GRCm39)	missense	probably damaging	1.00
R6947:Pcnx2	UTSW	8	126,577,021 (GRCm39)	critical splice donor site	probably null
R7034:Pcnx2	UTSW	8	126,512,041 (GRCm39)	missense	probably damaging	1.00
R7100:Pcnx2	UTSW	8	126,485,853 (GRCm39)	missense	probably benign	0.16
R7124:Pcnx2	UTSW	8	126,480,356 (GRCm39)	missense	probably damaging	0.99
R7130:Pcnx2	UTSW	8	126,480,323 (GRCm39)	nonsense	probably null
R7133:Pcnx2	UTSW	8	126,528,243 (GRCm39)	missense	probably benign	0.01
R7271:Pcnx2	UTSW	8	126,613,690 (GRCm39)	missense	probably benign
R7326:Pcnx2	UTSW	8	126,613,822 (GRCm39)	missense	probably damaging	1.00
R7373:Pcnx2	UTSW	8	126,534,766 (GRCm39)	missense	probably damaging	1.00
R7397:Pcnx2	UTSW	8	126,617,624 (GRCm39)	splice site	probably null
R7662:Pcnx2	UTSW	8	126,545,510 (GRCm39)	nonsense	probably null
R7693:Pcnx2	UTSW	8	126,613,864 (GRCm39)	missense	probably benign	0.09
R7726:Pcnx2	UTSW	8	126,577,069 (GRCm39)	missense	probably benign	0.00
R7745:Pcnx2	UTSW	8	126,577,846 (GRCm39)	missense	probably benign	0.04
R7792:Pcnx2	UTSW	8	126,618,757 (GRCm39)	missense	possibly damaging	0.63
R7797:Pcnx2	UTSW	8	126,512,087 (GRCm39)	missense	possibly damaging	0.70
R7921:Pcnx2	UTSW	8	126,564,602 (GRCm39)	missense	probably benign
R7984:Pcnx2	UTSW	8	126,485,865 (GRCm39)	missense	probably benign
R8098:Pcnx2	UTSW	8	126,495,040 (GRCm39)	missense	probably damaging	1.00
R8277:Pcnx2	UTSW	8	126,592,755 (GRCm39)	missense	probably damaging	1.00
R8312:Pcnx2	UTSW	8	126,489,589 (GRCm39)	missense	possibly damaging	0.69
R8354:Pcnx2	UTSW	8	126,488,357 (GRCm39)	missense	probably damaging	0.99
R8378:Pcnx2	UTSW	8	126,487,649 (GRCm39)	missense	probably damaging	1.00
R8713:Pcnx2	UTSW	8	126,545,525 (GRCm39)	missense	probably damaging	1.00
R8714:Pcnx2	UTSW	8	126,500,546 (GRCm39)	missense	probably benign
R8753:Pcnx2	UTSW	8	126,613,999 (GRCm39)	missense	probably benign	0.15
R8790:Pcnx2	UTSW	8	126,604,306 (GRCm39)	missense	probably benign
R8925:Pcnx2	UTSW	8	126,614,659 (GRCm39)	missense	probably benign	0.01
R8927:Pcnx2	UTSW	8	126,614,659 (GRCm39)	missense	probably benign	0.01
R8965:Pcnx2	UTSW	8	126,485,853 (GRCm39)	missense	probably benign	0.16
R9006:Pcnx2	UTSW	8	126,613,996 (GRCm39)	missense	probably benign	0.00
R9082:Pcnx2	UTSW	8	126,613,753 (GRCm39)	missense	probably damaging	1.00
R9202:Pcnx2	UTSW	8	126,616,416 (GRCm39)	critical splice acceptor site	probably null
R9315:Pcnx2	UTSW	8	126,614,119 (GRCm39)	missense	probably benign	0.00
R9434:Pcnx2	UTSW	8	126,542,512 (GRCm39)	missense	probably benign	0.00
R9660:Pcnx2	UTSW	8	126,487,592 (GRCm39)	missense	probably damaging	1.00
R9686:Pcnx2	UTSW	8	126,592,766 (GRCm39)	missense	probably benign
R9766:Pcnx2	UTSW	8	126,488,313 (GRCm39)	missense	probably damaging	1.00
R9778:Pcnx2	UTSW	8	126,512,176 (GRCm39)	missense	probably benign	0.00
R9792:Pcnx2	UTSW	8	126,534,820 (GRCm39)	missense	probably damaging	0.99
RF018:Pcnx2	UTSW	8	126,604,258 (GRCm39)	missense	probably damaging	1.00
Z1088:Pcnx2	UTSW	8	126,592,757 (GRCm39)	missense	probably damaging	1.00
Z1088:Pcnx2	UTSW	8	126,553,667 (GRCm39)	missense	probably damaging	1.00
Z1176:Pcnx2	UTSW	8	126,564,753 (GRCm39)	missense	probably benign	0.30
Z1176:Pcnx2	UTSW	8	126,488,393 (GRCm39)	missense	probably damaging	1.00
Z1177:Pcnx2	UTSW	8	126,614,699 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- ATTGAGCTCAGAGTCTTCTACAG -3'
(R):5'- GTGGTGTCATGGAGTCAAAAC -3'

Sequencing Primer
(F):5'- TCTTCTACAGACACAAGCCAAGAGG -3'
(R):5'- CAAAAGGTACAACTTGGAAATTGC -3'

Posted On

2016-02-04