Incidental Mutation 'R1666:Zfp740'
ID187229
Institutional Source Beutler Lab
Gene Symbol Zfp740
Ensembl Gene ENSMUSG00000046897
Gene Namezinc finger protein 740
Synonyms
MMRRC Submission 039702-MU
Accession Numbers
Is this an essential gene? Probably non essential (E-score: 0.189) question?
Stock #R1666 (G1)
Quality Score225
Status Not validated
Chromosome15
Chromosomal Location102203249-102215606 bp(+) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) A to G at 102208318 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 56 (D56G)
Ref Sequence ENSEMBL: ENSMUSP00000122429 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000118729] [ENSMUST00000119168] [ENSMUST00000119800] [ENSMUST00000121206] [ENSMUST00000133986] [ENSMUST00000139960] [ENSMUST00000141465] [ENSMUST00000229043] [ENSMUST00000229252] [ENSMUST00000229345] [ENSMUST00000229514] [ENSMUST00000229770] [ENSMUST00000229886] [ENSMUST00000230656] [ENSMUST00000230687] [ENSMUST00000230867] [ENSMUST00000231030]
Predicted Effect probably benign
Transcript: ENSMUST00000118729
AA Change: D44G

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)
SMART Domains Protein: ENSMUSP00000112985
Gene: ENSMUSG00000046897
AA Change: D44G

DomainStartEndE-ValueType
low complexity region 53 62 N/A INTRINSIC
ZnF_C2H2 76 98 3.07e-1 SMART
ZnF_C2H2 104 126 1.69e-3 SMART
ZnF_C2H2 132 152 4.99e1 SMART
Predicted Effect probably damaging
Transcript: ENSMUST00000119168
AA Change: D56G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
Predicted Effect probably benign
Transcript: ENSMUST00000119800
AA Change: D56G

PolyPhen 2 Score 0.216 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000113770
Gene: ENSMUSG00000046897
AA Change: D56G

DomainStartEndE-ValueType
low complexity region 65 74 N/A INTRINSIC
ZnF_C2H2 88 110 3.07e-1 SMART
ZnF_C2H2 116 138 1.69e-3 SMART
ZnF_C2H2 144 164 4.99e1 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000119830
SMART Domains Protein: ENSMUSP00000113281
Gene: ENSMUSG00000046897

DomainStartEndE-ValueType
ZnF_C2H2 25 47 3.07e-1 SMART
ZnF_C2H2 53 75 1.69e-3 SMART
ZnF_C2H2 81 101 4.99e1 SMART
Predicted Effect noncoding transcript
Transcript: ENSMUST00000121206
Predicted Effect noncoding transcript
Transcript: ENSMUST00000123528
Predicted Effect probably damaging
Transcript: ENSMUST00000133986
AA Change: D56G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000122429
Gene: ENSMUSG00000046897
AA Change: D56G

DomainStartEndE-ValueType
low complexity region 65 74 N/A INTRINSIC
Predicted Effect possibly damaging
Transcript: ENSMUST00000139960
AA Change: D56G

PolyPhen 2 Score 0.653 (Sensitivity: 0.87; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000117024
Gene: ENSMUSG00000046897
AA Change: D56G

DomainStartEndE-ValueType
low complexity region 65 74 N/A INTRINSIC
Blast:ZnF_C2H2 88 107 9e-7 BLAST
Predicted Effect probably benign
Transcript: ENSMUST00000141465
AA Change: D81G

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)
Predicted Effect probably benign
Transcript: ENSMUST00000229043
Predicted Effect probably benign
Transcript: ENSMUST00000229252
Predicted Effect probably benign
Transcript: ENSMUST00000229345
Predicted Effect probably benign
Transcript: ENSMUST00000229514
Predicted Effect probably benign
Transcript: ENSMUST00000229770
Predicted Effect possibly damaging
Transcript: ENSMUST00000229886
AA Change: D44G

PolyPhen 2 Score 0.904 (Sensitivity: 0.82; Specificity: 0.94)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230008
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230328
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230600
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230644
Predicted Effect probably benign
Transcript: ENSMUST00000230656
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230665
Predicted Effect probably benign
Transcript: ENSMUST00000230687
Predicted Effect probably benign
Transcript: ENSMUST00000230867
AA Change: D19G

PolyPhen 2 Score 0.034 (Sensitivity: 0.95; Specificity: 0.82)
Predicted Effect noncoding transcript
Transcript: ENSMUST00000230895
Predicted Effect probably benign
Transcript: ENSMUST00000231030
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.7%
  • 20x: 93.6%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 79 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
5330417C22Rik A G 3: 108,469,997 S434P probably benign Het
Acvrl1 G A 15: 101,137,577 R328H probably damaging Het
Afp T C 5: 90,505,068 S466P probably damaging Het
Arhgap45 A G 10: 80,028,750 S879G possibly damaging Het
Asic1 A G 15: 99,699,125 D556G probably damaging Het
Atp2a3 T A 11: 72,978,807 probably null Het
Brinp2 C A 1: 158,246,558 E664D probably damaging Het
Cap2 C A 13: 46,615,323 H147N probably damaging Het
Cd209f G A 8: 4,104,862 Q79* probably null Het
Chrna7 T C 7: 63,212,142 Y54C possibly damaging Het
Clec1a T C 6: 129,437,004 D40G probably benign Het
Col11a1 A G 3: 114,061,535 E148G unknown Het
Comp A T 8: 70,378,957 probably null Het
Cpz A G 5: 35,508,116 probably null Het
Cyfip1 T A 7: 55,871,898 N13K probably damaging Het
Cyp2c50 A T 19: 40,091,055 M198L probably benign Het
Deup1 A T 9: 15,575,191 Y398N possibly damaging Het
Epb41l4a A G 18: 33,921,909 L42P probably damaging Het
Exo1 T C 1: 175,908,486 I812T possibly damaging Het
Fbxl17 A T 17: 63,385,065 probably null Het
Fxn A G 19: 24,262,013 Y172H probably damaging Het
Gabra6 A G 11: 42,317,634 S124P probably damaging Het
Gje1 C A 10: 14,716,807 W77L possibly damaging Het
Glra1 A G 11: 55,574,399 S23P probably damaging Het
Gm6614 T A 6: 141,982,049 probably null Het
Gm6811 T C 17: 21,094,267 noncoding transcript Het
Greb1l T A 18: 10,501,080 probably null Het
Greb1l T C 18: 10,529,708 probably null Het
Grm6 A T 11: 50,859,884 I625F probably damaging Het
Guca1a A G 17: 47,400,242 F60L probably damaging Het
Hectd1 T A 12: 51,753,824 E2070D possibly damaging Het
Itga5 G A 15: 103,347,902 T910I probably benign Het
Kctd10 A G 5: 114,368,990 V142A probably benign Het
Kif1c C T 11: 70,728,397 L953F probably damaging Het
Lmtk3 A T 7: 45,794,164 D757V probably benign Het
Lpcat1 T C 13: 73,510,123 probably null Het
Lrrc30 A T 17: 67,632,205 C127S probably benign Het
Mc4r A G 18: 66,859,409 L211P probably damaging Het
Mettl7a3 A G 15: 100,335,218 I97V probably benign Het
Mgat5b T A 11: 116,983,648 N635K probably benign Het
Mms19 A T 19: 41,952,556 M443K possibly damaging Het
Mroh5 T C 15: 73,787,905 N359S probably benign Het
Nlrc4 G A 17: 74,445,906 T494M probably damaging Het
Ntf3 T C 6: 126,102,438 D35G possibly damaging Het
Olfr1002 A T 2: 85,647,813 C169* probably null Het
Osbpl5 T C 7: 143,709,039 H192R probably damaging Het
Parp4 A T 14: 56,624,163 K984N possibly damaging Het
Pdgfra G A 5: 75,189,020 G892D possibly damaging Het
Pik3c2g A T 6: 139,635,636 probably benign Het
Ppcdc T A 9: 57,414,715 M181L possibly damaging Het
Pramel7 G A 2: 87,492,403 P6S probably damaging Het
Prkcz G T 4: 155,289,751 F69L probably damaging Het
Prkd1 G A 12: 50,394,926 H277Y probably damaging Het
Ptgs2 A G 1: 150,101,270 Y44C probably damaging Het
Rbck1 T A 2: 152,316,899 S488C probably damaging Het
Rbl1 T C 2: 157,159,734 Y878C probably damaging Het
Rbm6 G A 9: 107,791,856 T619I probably benign Het
Rp1 A G 1: 4,349,863 I342T probably damaging Het
Rpn2 C T 2: 157,294,155 T161M possibly damaging Het
Rufy4 G A 1: 74,147,678 V542I probably benign Het
Sema5b C T 16: 35,658,482 P559S probably benign Het
Serpinb3d A G 1: 107,080,751 V128A probably benign Het
Slc35f3 T A 8: 126,389,221 S296T probably damaging Het
Smarca2 A G 19: 26,647,034 I365V possibly damaging Het
Spdya T A 17: 71,578,240 C230S probably damaging Het
Sv2b G A 7: 75,206,341 A67V probably benign Het
Tapbpl A G 6: 125,230,201 V175A probably benign Het
Ttn T C 2: 76,811,751 T13373A probably damaging Het
Tubb5 A T 17: 35,836,638 N52K probably benign Het
Tyr T A 7: 87,492,941 Y137F probably damaging Het
Unc45b T A 11: 82,917,739 I217N probably benign Het
Vmn1r202 A T 13: 22,501,370 D292E possibly damaging Het
Vmn1r22 A G 6: 57,900,719 M91T probably benign Het
Washc2 T A 6: 116,223,254 probably null Het
Zfp566 A T 7: 30,078,476 H93Q probably benign Het
Zfp804b A C 5: 6,771,323 L580R possibly damaging Het
Zmiz2 T G 11: 6,396,836 S148R probably benign Het
Zmym6 T A 4: 127,122,859 I719N probably damaging Het
Zyx T A 6: 42,356,032 V372E possibly damaging Het
Other mutations in Zfp740
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL02165:Zfp740 APN 15 102212674 missense possibly damaging 0.66
R0432:Zfp740 UTSW 15 102212659 missense possibly damaging 0.66
R0659:Zfp740 UTSW 15 102212659 missense possibly damaging 0.66
R0661:Zfp740 UTSW 15 102212659 missense possibly damaging 0.66
R1830:Zfp740 UTSW 15 102207901 missense probably damaging 1.00
R3983:Zfp740 UTSW 15 102208243 missense probably benign 0.06
R4686:Zfp740 UTSW 15 102208749 unclassified probably benign
R4991:Zfp740 UTSW 15 102208279 unclassified probably null
R5170:Zfp740 UTSW 15 102209205 missense probably damaging 1.00
R5213:Zfp740 UTSW 15 102212647 missense possibly damaging 0.68
R5782:Zfp740 UTSW 15 102208366 unclassified probably benign
R6088:Zfp740 UTSW 15 102208808 missense probably damaging 1.00
R6848:Zfp740 UTSW 15 102208808 missense probably benign 0.24
Predicted Primers PCR Primer
(F):5'- AGCTCTCTGCATAGAGGCACCAAG -3'
(R):5'- ATGTTCAGCAAGAGTGGACGGCAAC -3'

Sequencing Primer
(F):5'- TGCAGACTCTTACTTAGACCAGG -3'
(R):5'- CTGTCAGAGTGGCCTCAGATTC -3'
Posted On2014-05-09