Mutagenetix > Incidental Mutation

Incidental Mutation 'R5065:Mroh4'

388308

Institutional Source

Beutler Lab

Gene Symbol

Mroh4

Ensembl Gene

ENSMUSG00000022603

Gene Name

maestro heat-like repeat family member 4

Synonyms

1700016M24Rik

MMRRC Submission

042655-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5065 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

74477878-74508202 bp(-) (GRCm39)

Type of Mutation

splice site (5 bp from exon)

DNA Base Change (assembly)

C to T at 74500119 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000117011 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023271] [ENSMUST00000137963]

AlphaFold

G3X8W1

Predicted Effect

probably null
Transcript: ENSMUST00000023271

SMART Domains

Protein: ENSMUSP00000023271
Gene: ENSMUSG00000022603

Domain	Start	End	E-Value	Type
low complexity region	1	12	N/A	INTRINSIC
low complexity region	326	337	N/A	INTRINSIC
low complexity region	428	435	N/A	INTRINSIC
low complexity region	520	534	N/A	INTRINSIC
low complexity region	572	591	N/A	INTRINSIC
SCOP:d1ee4a_	709	852	3e-5	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000137963

SMART Domains

Protein: ENSMUSP00000117011
Gene: ENSMUSG00000022603

Domain	Start	End	E-Value	Type
low complexity region	257	268	N/A	INTRINSIC
low complexity region	359	366	N/A	INTRINSIC
low complexity region	451	465	N/A	INTRINSIC
low complexity region	503	522	N/A	INTRINSIC
SCOP:d1ee4a_	640	783	3e-5	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000175931

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176767

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.0%

Validation Efficiency

100% (36/36)

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310009B15Rik	T	A	1: 138,779,893 (GRCm39)	N120Y	probably damaging	Het
A930011G23Rik	G	A	5: 99,382,432 (GRCm39)	T272M	probably benign	Het
Aldh9a1	A	G	1: 167,180,128 (GRCm39)	E74G	probably damaging	Het
Asxl3	C	G	18: 22,658,356 (GRCm39)	A2122G	possibly damaging	Het
Bcl2l10	A	T	9: 75,255,261 (GRCm39)	E26V	possibly damaging	Het
Cspg4b	T	A	13: 113,457,453 (GRCm39)	H1166Q	probably benign	Het
Dennd2a	C	A	6: 39,472,110 (GRCm39)		probably null	Het
Dock3	A	G	9: 106,832,883 (GRCm39)	F129L	probably damaging	Het
Gm4847	A	T	1: 166,462,359 (GRCm39)	I377N	probably damaging	Het
Gm8674	T	C	13: 50,056,613 (GRCm39)		noncoding transcript	Het
Hhipl2	A	T	1: 183,207,580 (GRCm39)	H433L	probably benign	Het
Hsfy2	G	A	1: 56,675,626 (GRCm39)	Q304*	probably null	Het
Ighg2c	T	C	12: 113,251,708 (GRCm39)	I140V	unknown	Het
Kmt2a	T	C	9: 44,753,997 (GRCm39)		probably benign	Het
Map3k5	A	G	10: 19,958,213 (GRCm39)	E671G	probably damaging	Het
Map6	A	G	7: 98,985,917 (GRCm39)	D607G	probably benign	Het
Or10g6	A	G	9: 39,934,546 (GRCm39)	I286V	probably benign	Het
Pcsk6	A	G	7: 65,560,047 (GRCm39)	D124G	possibly damaging	Het
Pkhd1l1	T	A	15: 44,445,689 (GRCm39)	N3790K	possibly damaging	Het
Polr3b	C	A	10: 84,468,402 (GRCm39)	N129K	probably benign	Het
Ptpn23	A	T	9: 110,227,256 (GRCm39)	L31Q	possibly damaging	Het
Sema3c	A	G	5: 17,932,615 (GRCm39)	N706S	possibly damaging	Het
Sipa1l2	T	C	8: 126,218,324 (GRCm39)	I338V	probably benign	Het
Slc39a7	A	G	17: 34,250,033 (GRCm39)		probably benign	Het
Snd1	C	A	6: 28,888,239 (GRCm39)	N891K	probably damaging	Het
Sntg1	A	G	1: 8,433,663 (GRCm39)		probably benign	Het
Stxbp5	A	T	10: 9,646,295 (GRCm39)	L780Q	probably damaging	Het
Tacr1	G	A	6: 82,531,859 (GRCm39)	V252M	possibly damaging	Het
Tdrp	T	C	8: 14,003,791 (GRCm39)	E182G	probably damaging	Het
Vmn1r58	A	G	7: 5,413,834 (GRCm39)	I132T	probably benign	Het
Wap	A	T	11: 6,586,840 (GRCm39)	N86K	probably damaging	Het

Other mutations in Mroh4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01645:Mroh4	APN	15	74,483,207 (GRCm39)	splice site	probably benign
IGL02370:Mroh4	APN	15	74,497,390 (GRCm39)	missense	probably benign	0.00
IGL02598:Mroh4	APN	15	74,483,092 (GRCm39)	critical splice donor site	probably null
IGL02644:Mroh4	APN	15	74,482,224 (GRCm39)	missense	possibly damaging	0.90
IGL02666:Mroh4	APN	15	74,481,624 (GRCm39)	missense	probably benign	0.04
IGL02723:Mroh4	APN	15	74,480,086 (GRCm39)	splice site	probably benign
IGL02724:Mroh4	APN	15	74,478,000 (GRCm39)	missense	probably benign	0.00
IGL03000:Mroh4	APN	15	74,487,963 (GRCm39)	missense	probably benign
IGL03103:Mroh4	APN	15	74,488,008 (GRCm39)	missense	possibly damaging	0.47
IGL03194:Mroh4	APN	15	74,483,388 (GRCm39)	missense	probably damaging	1.00
R0013:Mroh4	UTSW	15	74,480,086 (GRCm39)	splice site	probably benign
R0042:Mroh4	UTSW	15	74,482,154 (GRCm39)	missense	probably damaging	0.99
R0042:Mroh4	UTSW	15	74,482,154 (GRCm39)	missense	probably damaging	0.99
R0294:Mroh4	UTSW	15	74,477,998 (GRCm39)	missense	probably benign
R0346:Mroh4	UTSW	15	74,486,141 (GRCm39)	splice site	probably benign
R0545:Mroh4	UTSW	15	74,497,276 (GRCm39)	missense	probably benign	0.00
R0688:Mroh4	UTSW	15	74,478,527 (GRCm39)	missense	probably damaging	0.98
R1838:Mroh4	UTSW	15	74,487,962 (GRCm39)	missense	probably benign	0.03
R2037:Mroh4	UTSW	15	74,481,610 (GRCm39)	missense	possibly damaging	0.91
R4725:Mroh4	UTSW	15	74,487,956 (GRCm39)	missense	probably damaging	0.99
R4786:Mroh4	UTSW	15	74,482,083 (GRCm39)	missense	probably benign	0.08
R4798:Mroh4	UTSW	15	74,498,028 (GRCm39)	missense	probably damaging	1.00
R4945:Mroh4	UTSW	15	74,483,857 (GRCm39)	missense	probably benign	0.00
R5476:Mroh4	UTSW	15	74,483,510 (GRCm39)	missense	probably benign	0.15
R5509:Mroh4	UTSW	15	74,478,003 (GRCm39)	missense	probably benign	0.00
R5527:Mroh4	UTSW	15	74,486,865 (GRCm39)	missense	probably damaging	1.00
R5662:Mroh4	UTSW	15	74,497,277 (GRCm39)	missense	possibly damaging	0.63
R5818:Mroh4	UTSW	15	74,483,831 (GRCm39)	missense	probably damaging	0.98
R5861:Mroh4	UTSW	15	74,478,456 (GRCm39)	intron	probably benign
R5886:Mroh4	UTSW	15	74,478,296 (GRCm39)	missense	possibly damaging	0.90
R5935:Mroh4	UTSW	15	74,493,003 (GRCm39)	missense	probably damaging	1.00
R6008:Mroh4	UTSW	15	74,497,321 (GRCm39)	nonsense	probably null
R6658:Mroh4	UTSW	15	74,492,978 (GRCm39)	missense	possibly damaging	0.83
R6689:Mroh4	UTSW	15	74,483,852 (GRCm39)	missense	probably damaging	1.00
R6739:Mroh4	UTSW	15	74,481,568 (GRCm39)	missense	probably benign	0.10
R6888:Mroh4	UTSW	15	74,485,098 (GRCm39)	missense	possibly damaging	0.93
R7088:Mroh4	UTSW	15	74,497,993 (GRCm39)	missense	probably benign	0.25
R7260:Mroh4	UTSW	15	74,479,978 (GRCm39)	missense	possibly damaging	0.83
R7365:Mroh4	UTSW	15	74,482,220 (GRCm39)	nonsense	probably null
R7735:Mroh4	UTSW	15	74,497,357 (GRCm39)	missense	probably damaging	0.98
R7763:Mroh4	UTSW	15	74,496,554 (GRCm39)	missense	probably damaging	0.99
R7945:Mroh4	UTSW	15	74,496,554 (GRCm39)	missense	probably damaging	0.99
R8090:Mroh4	UTSW	15	74,496,550 (GRCm39)	missense	probably benign	0.41
R8242:Mroh4	UTSW	15	74,488,157 (GRCm39)	missense	possibly damaging	0.47
R8978:Mroh4	UTSW	15	74,499,473 (GRCm39)	missense	probably benign	0.00
R9004:Mroh4	UTSW	15	74,486,171 (GRCm39)	missense	possibly damaging	0.65
R9083:Mroh4	UTSW	15	74,498,140 (GRCm39)	missense	probably damaging	1.00
R9172:Mroh4	UTSW	15	74,477,961 (GRCm39)	makesense	probably null
R9248:Mroh4	UTSW	15	74,485,167 (GRCm39)	missense	possibly damaging	0.59
R9320:Mroh4	UTSW	15	74,483,405 (GRCm39)	missense	probably damaging	1.00
R9356:Mroh4	UTSW	15	74,482,760 (GRCm39)	missense	probably benign	0.05
R9512:Mroh4	UTSW	15	74,485,095 (GRCm39)	missense	probably benign	0.18
Z1177:Mroh4	UTSW	15	74,499,851 (GRCm39)	missense	possibly damaging	0.83
Z1177:Mroh4	UTSW	15	74,499,569 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAGGCTAATGCTGCTCAGAAAC -3'
(R):5'- GCTGTCCTCCTAGACACAGATC -3'

Sequencing Primer
(F):5'- GCTAATGCTGCTCAGAAACACACC -3'
(R):5'- TAGACACAGATCTCGGGATCCTG -3'

Posted On

2016-06-06