Mutagenetix > Incidental Mutation

Incidental Mutation 'R5107:Col18a1'

393613

Institutional Source

Beutler Lab

Gene Symbol

Col18a1

Ensembl Gene

ENSMUSG00000001435

Gene Name

collagen, type XVIII, alpha 1

Synonyms

endostatin

MMRRC Submission

042695-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5107 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

76888013-77002351 bp(-) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

T to C at 76913057 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000101049 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072755] [ENSMUST00000072755] [ENSMUST00000081654] [ENSMUST00000081654] [ENSMUST00000105409] [ENSMUST00000105409] [ENSMUST00000156009]

AlphaFold

no structure available at present

Predicted Effect

probably null
Transcript: ENSMUST00000072755

SMART Domains

Protein: ENSMUSP00000072538
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
Pfam:DUF959	16	218	6.8e-104	PFAM
low complexity region	295	307	N/A	INTRINSIC
FRI	369	484	4.03e-47	SMART
TSPN	492	680	4.25e-72	SMART
LamG	541	679	2.17e-2	SMART
low complexity region	699	715	N/A	INTRINSIC
low complexity region	719	734	N/A	INTRINSIC
low complexity region	739	751	N/A	INTRINSIC
Pfam:Collagen	820	881	5.5e-11	PFAM
low complexity region	921	942	N/A	INTRINSIC
Pfam:Collagen	951	1008	6.1e-10	PFAM
Pfam:Collagen	988	1053	1.4e-8	PFAM
Pfam:Collagen	1060	1117	7.3e-10	PFAM
low complexity region	1132	1147	N/A	INTRINSIC
low complexity region	1166	1181	N/A	INTRINSIC
low complexity region	1186	1202	N/A	INTRINSIC
Pfam:Collagen	1207	1267	8.2e-10	PFAM
low complexity region	1275	1288	N/A	INTRINSIC
low complexity region	1301	1319	N/A	INTRINSIC
low complexity region	1358	1393	N/A	INTRINSIC
low complexity region	1397	1414	N/A	INTRINSIC
low complexity region	1417	1433	N/A	INTRINSIC
low complexity region	1441	1454	N/A	INTRINSIC
Pfam:Endostatin	1461	1769	4.4e-116	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000072755

SMART Domains

Protein: ENSMUSP00000072538
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
Pfam:DUF959	16	218	6.8e-104	PFAM
low complexity region	295	307	N/A	INTRINSIC
FRI	369	484	4.03e-47	SMART
TSPN	492	680	4.25e-72	SMART
LamG	541	679	2.17e-2	SMART
low complexity region	699	715	N/A	INTRINSIC
low complexity region	719	734	N/A	INTRINSIC
low complexity region	739	751	N/A	INTRINSIC
Pfam:Collagen	820	881	5.5e-11	PFAM
low complexity region	921	942	N/A	INTRINSIC
Pfam:Collagen	951	1008	6.1e-10	PFAM
Pfam:Collagen	988	1053	1.4e-8	PFAM
Pfam:Collagen	1060	1117	7.3e-10	PFAM
low complexity region	1132	1147	N/A	INTRINSIC
low complexity region	1166	1181	N/A	INTRINSIC
low complexity region	1186	1202	N/A	INTRINSIC
Pfam:Collagen	1207	1267	8.2e-10	PFAM
low complexity region	1275	1288	N/A	INTRINSIC
low complexity region	1301	1319	N/A	INTRINSIC
low complexity region	1358	1393	N/A	INTRINSIC
low complexity region	1397	1414	N/A	INTRINSIC
low complexity region	1417	1433	N/A	INTRINSIC
low complexity region	1441	1454	N/A	INTRINSIC
Pfam:Endostatin	1461	1769	4.4e-116	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000081654

SMART Domains

Protein: ENSMUSP00000080358
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
TSPN	33	221	4.25e-72	SMART
LamG	82	220	2.17e-2	SMART
low complexity region	240	256	N/A	INTRINSIC
low complexity region	260	275	N/A	INTRINSIC
low complexity region	280	292	N/A	INTRINSIC
Pfam:Collagen	359	422	1.6e-10	PFAM
low complexity region	462	483	N/A	INTRINSIC
Pfam:Collagen	492	549	1.6e-9	PFAM
Pfam:Collagen	529	594	3.3e-8	PFAM
Pfam:Collagen	601	658	1.9e-9	PFAM
Pfam:Collagen	631	689	4e-8	PFAM
Pfam:Collagen	701	752	1.7e-7	PFAM
Pfam:Collagen	748	808	2.2e-9	PFAM
low complexity region	816	829	N/A	INTRINSIC
low complexity region	842	860	N/A	INTRINSIC
low complexity region	899	934	N/A	INTRINSIC
low complexity region	938	955	N/A	INTRINSIC
low complexity region	958	974	N/A	INTRINSIC
low complexity region	982	995	N/A	INTRINSIC
Pfam:Endostatin	999	1315	8.2e-151	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000081654

SMART Domains

Protein: ENSMUSP00000080358
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
TSPN	33	221	4.25e-72	SMART
LamG	82	220	2.17e-2	SMART
low complexity region	240	256	N/A	INTRINSIC
low complexity region	260	275	N/A	INTRINSIC
low complexity region	280	292	N/A	INTRINSIC
Pfam:Collagen	359	422	1.6e-10	PFAM
low complexity region	462	483	N/A	INTRINSIC
Pfam:Collagen	492	549	1.6e-9	PFAM
Pfam:Collagen	529	594	3.3e-8	PFAM
Pfam:Collagen	601	658	1.9e-9	PFAM
Pfam:Collagen	631	689	4e-8	PFAM
Pfam:Collagen	701	752	1.7e-7	PFAM
Pfam:Collagen	748	808	2.2e-9	PFAM
low complexity region	816	829	N/A	INTRINSIC
low complexity region	842	860	N/A	INTRINSIC
low complexity region	899	934	N/A	INTRINSIC
low complexity region	938	955	N/A	INTRINSIC
low complexity region	958	974	N/A	INTRINSIC
low complexity region	982	995	N/A	INTRINSIC
Pfam:Endostatin	999	1315	8.2e-151	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000105409

SMART Domains

Protein: ENSMUSP00000101049
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
Pfam:DUF959	16	219	3.6e-100	PFAM
TSPN	245	433	4.25e-72	SMART
LamG	294	432	2.17e-2	SMART
low complexity region	452	468	N/A	INTRINSIC
low complexity region	472	487	N/A	INTRINSIC
low complexity region	492	504	N/A	INTRINSIC
low complexity region	542	613	N/A	INTRINSIC
low complexity region	630	648	N/A	INTRINSIC
low complexity region	674	695	N/A	INTRINSIC
Pfam:Collagen	700	761	5.4e-9	PFAM
Pfam:Collagen	741	806	4e-8	PFAM
Pfam:Collagen	813	874	2.1e-10	PFAM
Pfam:Collagen	846	901	1.2e-7	PFAM
Pfam:Collagen	913	964	2.1e-7	PFAM
Pfam:Collagen	960	1020	2.6e-9	PFAM
low complexity region	1028	1041	N/A	INTRINSIC
low complexity region	1054	1072	N/A	INTRINSIC
low complexity region	1111	1146	N/A	INTRINSIC
low complexity region	1150	1167	N/A	INTRINSIC
low complexity region	1170	1186	N/A	INTRINSIC
low complexity region	1194	1207	N/A	INTRINSIC
Pfam:Endostatin	1211	1527	1.1e-150	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000105409

SMART Domains

Protein: ENSMUSP00000101049
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
Pfam:DUF959	16	219	3.6e-100	PFAM
TSPN	245	433	4.25e-72	SMART
LamG	294	432	2.17e-2	SMART
low complexity region	452	468	N/A	INTRINSIC
low complexity region	472	487	N/A	INTRINSIC
low complexity region	492	504	N/A	INTRINSIC
low complexity region	542	613	N/A	INTRINSIC
low complexity region	630	648	N/A	INTRINSIC
low complexity region	674	695	N/A	INTRINSIC
Pfam:Collagen	700	761	5.4e-9	PFAM
Pfam:Collagen	741	806	4e-8	PFAM
Pfam:Collagen	813	874	2.1e-10	PFAM
Pfam:Collagen	846	901	1.2e-7	PFAM
Pfam:Collagen	913	964	2.1e-7	PFAM
Pfam:Collagen	960	1020	2.6e-9	PFAM
low complexity region	1028	1041	N/A	INTRINSIC
low complexity region	1054	1072	N/A	INTRINSIC
low complexity region	1111	1146	N/A	INTRINSIC
low complexity region	1150	1167	N/A	INTRINSIC
low complexity region	1170	1186	N/A	INTRINSIC
low complexity region	1194	1207	N/A	INTRINSIC
Pfam:Endostatin	1211	1527	1.1e-150	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000156009

SMART Domains

Protein: ENSMUSP00000122580
Gene: ENSMUSG00000001435

Domain	Start	End	E-Value	Type
low complexity region	30	46	N/A	INTRINSIC
low complexity region	50	65	N/A	INTRINSIC
low complexity region	70	82	N/A	INTRINSIC
low complexity region	120	161	N/A	INTRINSIC

Meta Mutation Damage Score

0.9496

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.3%
20x: 92.7%

Validation Efficiency

98% (56/57)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit defects in hyaloid vessel regression, attenuated visual function, abnormal electroretinograms, broad proximal tubule basement membrane, podocyte effacement, and softened glomeruli. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930486L24Rik	T	C	13: 61,001,472 (GRCm39)	N85S	possibly damaging	Het
Adgre1	A	G	17: 57,708,977 (GRCm39)	Y56C	possibly damaging	Het
Agbl5	C	A	5: 31,049,822 (GRCm39)	P257Q	probably damaging	Het
Angptl2	A	T	2: 33,118,615 (GRCm39)	M130L	probably damaging	Het
Atp6v1f	T	A	6: 29,468,198 (GRCm39)		probably null	Het
Ccdc78	G	A	17: 26,006,454 (GRCm39)	V133M	possibly damaging	Het
Clpx	A	G	9: 65,215,821 (GRCm39)	K145E	possibly damaging	Het
Dnase1l1	C	T	X: 73,320,644 (GRCm39)		probably null	Het
Igkv4-54	T	C	6: 69,608,914 (GRCm39)	I24V	possibly damaging	Het
Ikzf3	A	G	11: 98,381,302 (GRCm39)	Y93H	probably damaging	Het
Isl2	T	C	9: 55,449,570 (GRCm39)	V82A	probably benign	Het
Kcnq2	A	T	2: 180,750,340 (GRCm39)		probably benign	Het
Krt4	A	T	15: 101,831,226 (GRCm39)	M225K	possibly damaging	Het
Ksr2	T	C	5: 117,827,673 (GRCm39)	V484A	probably benign	Het
Lrba	T	A	3: 86,267,086 (GRCm39)	V1592E	possibly damaging	Het
Lrrc7	G	A	3: 157,867,533 (GRCm39)	P736L	probably damaging	Het
Mettl8	A	T	2: 70,795,901 (GRCm39)	F376I	probably damaging	Het
Muc5b	T	A	7: 141,409,268 (GRCm39)	H1117Q	unknown	Het
Myh7	T	A	14: 55,223,881 (GRCm39)		probably benign	Het
Myod1	G	T	7: 46,027,218 (GRCm39)	A228S	probably benign	Het
Ntrk1	T	C	3: 87,702,280 (GRCm39)	T58A	probably benign	Het
Or12d17	A	G	17: 37,777,144 (GRCm39)	T16A	probably damaging	Het
Pdcd11	T	A	19: 47,094,893 (GRCm39)	V559E	probably damaging	Het
Pde10a	A	G	17: 9,163,802 (GRCm39)	N191S	probably damaging	Het
Phf3	A	G	1: 30,870,566 (GRCm39)	S161P	probably benign	Het
Pias1	C	A	9: 62,789,510 (GRCm39)	A566S	probably benign	Het
Pik3c2g	T	C	6: 139,612,623 (GRCm39)		probably benign	Het
Pmp22	A	G	11: 63,049,237 (GRCm39)	E160G	probably damaging	Het
Polb	T	C	8: 23,135,062 (GRCm39)		probably null	Het
Prob1	T	C	18: 35,785,989 (GRCm39)	N755S	possibly damaging	Het
Rhou	A	T	8: 124,387,912 (GRCm39)	K215*	probably null	Het
Scaper	A	G	9: 55,487,616 (GRCm39)	S749P	probably damaging	Het
Selenop	A	G	15: 3,305,075 (GRCm39)	E77G	probably damaging	Het
Sema3a	C	A	5: 13,627,572 (GRCm39)	S490*	probably null	Het
Slc26a2	A	G	18: 61,331,632 (GRCm39)	Y600H	probably damaging	Het
Slfn8	A	C	11: 82,907,976 (GRCm39)	I189S	probably damaging	Het
Stab1	T	A	14: 30,885,752 (GRCm39)		probably null	Het
Tbc1d13	G	A	2: 30,036,733 (GRCm39)	E205K	probably damaging	Het
Tex47	C	T	5: 7,354,842 (GRCm39)	R8W	probably benign	Het
Tmem198b	T	C	10: 128,638,156 (GRCm39)	T136A	probably benign	Het
Trim36	T	C	18: 46,305,705 (GRCm39)	Q414R	probably benign	Het
Ttc23l	G	T	15: 10,551,636 (GRCm39)	T30K	possibly damaging	Het
Ttn	A	G	2: 76,608,496 (GRCm39)	I16063T	probably damaging	Het
Ttn	A	G	2: 76,693,905 (GRCm39)	V321A	possibly damaging	Het
Ubox5	A	G	2: 130,441,688 (GRCm39)	L333P	probably damaging	Het
Vmn2r19	T	A	6: 123,286,602 (GRCm39)	Y78*	probably null	Het
Wnk4	A	G	11: 101,166,364 (GRCm39)		probably benign	Het
Xirp2	A	G	2: 67,340,054 (GRCm39)	D765G	probably damaging	Het
Xirp2	G	T	2: 67,342,205 (GRCm39)	G1482V	probably damaging	Het
Znhit1	A	G	5: 137,015,682 (GRCm39)	V2A	probably benign	Het

Other mutations in Col18a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00952:Col18a1	APN	10	76,905,813 (GRCm39)	missense	possibly damaging	0.93
IGL01023:Col18a1	APN	10	76,906,809 (GRCm39)	missense	probably damaging	0.98
IGL01304:Col18a1	APN	10	76,911,975 (GRCm39)	unclassified	probably benign
IGL01519:Col18a1	APN	10	76,895,157 (GRCm39)	missense	probably damaging	0.99
IGL02217:Col18a1	APN	10	76,889,132 (GRCm39)	missense	probably damaging	0.96
IGL02275:Col18a1	APN	10	76,895,217 (GRCm39)	missense	possibly damaging	0.92
IGL02283:Col18a1	APN	10	76,948,943 (GRCm39)	missense	possibly damaging	0.71
IGL02492:Col18a1	APN	10	76,907,855 (GRCm39)	splice site	probably benign
IGL02673:Col18a1	APN	10	76,894,997 (GRCm39)	missense	probably damaging	1.00
IGL02710:Col18a1	APN	10	76,949,146 (GRCm39)	missense	possibly damaging	0.92
IGL02850:Col18a1	APN	10	76,932,300 (GRCm39)	missense	probably damaging	0.98
IGL03085:Col18a1	APN	10	76,895,015 (GRCm39)	splice site	probably benign
IGL03102:Col18a1	APN	10	76,903,457 (GRCm39)	splice site	probably benign
IGL03139:Col18a1	APN	10	76,949,177 (GRCm39)	missense	possibly damaging	0.84
IGL03181:Col18a1	APN	10	76,891,532 (GRCm39)	missense	probably damaging	1.00
IGL03183:Col18a1	APN	10	76,909,588 (GRCm39)	missense	probably damaging	1.00
R0039:Col18a1	UTSW	10	76,913,002 (GRCm39)	missense	probably damaging	1.00
R0180:Col18a1	UTSW	10	76,932,351 (GRCm39)	missense	probably benign	0.33
R0225:Col18a1	UTSW	10	76,924,748 (GRCm39)	missense	possibly damaging	0.90
R0335:Col18a1	UTSW	10	76,895,197 (GRCm39)	missense	probably damaging	0.99
R0336:Col18a1	UTSW	10	76,894,570 (GRCm39)	missense	probably damaging	1.00
R1471:Col18a1	UTSW	10	76,932,040 (GRCm39)	missense	unknown
R1538:Col18a1	UTSW	10	76,907,170 (GRCm39)	missense	probably damaging	1.00
R1594:Col18a1	UTSW	10	76,948,870 (GRCm39)	missense	possibly damaging	0.51
R1631:Col18a1	UTSW	10	76,895,131 (GRCm39)	missense	probably damaging	0.99
R1774:Col18a1	UTSW	10	76,895,815 (GRCm39)	missense	probably damaging	0.96
R1934:Col18a1	UTSW	10	76,948,578 (GRCm39)	missense	possibly damaging	0.73
R1990:Col18a1	UTSW	10	76,916,988 (GRCm39)	missense	unknown
R1991:Col18a1	UTSW	10	76,916,988 (GRCm39)	missense	unknown
R1992:Col18a1	UTSW	10	76,916,988 (GRCm39)	missense	unknown
R2081:Col18a1	UTSW	10	76,890,019 (GRCm39)	missense	probably damaging	1.00
R2082:Col18a1	UTSW	10	76,895,127 (GRCm39)	missense	probably damaging	1.00
R2351:Col18a1	UTSW	10	76,948,538 (GRCm39)	missense	probably benign	0.00
R2510:Col18a1	UTSW	10	76,932,102 (GRCm39)	missense	unknown
R3076:Col18a1	UTSW	10	76,924,762 (GRCm39)	missense	possibly damaging	0.57
R3433:Col18a1	UTSW	10	76,932,102 (GRCm39)	missense	unknown
R3800:Col18a1	UTSW	10	76,903,221 (GRCm39)	nonsense	probably null
R3918:Col18a1	UTSW	10	76,889,192 (GRCm39)	missense	probably benign	0.05
R3981:Col18a1	UTSW	10	76,924,721 (GRCm39)	missense	probably damaging	0.99
R3983:Col18a1	UTSW	10	76,924,721 (GRCm39)	missense	probably damaging	0.99
R4182:Col18a1	UTSW	10	76,894,675 (GRCm39)	splice site	probably null
R4239:Col18a1	UTSW	10	76,932,001 (GRCm39)	missense	unknown
R5014:Col18a1	UTSW	10	76,906,794 (GRCm39)	critical splice donor site	probably null
R5413:Col18a1	UTSW	10	76,905,310 (GRCm39)	missense	probably damaging	1.00
R5503:Col18a1	UTSW	10	76,907,454 (GRCm39)	missense	probably damaging	1.00
R5524:Col18a1	UTSW	10	76,894,558 (GRCm39)	missense	probably damaging	1.00
R5772:Col18a1	UTSW	10	77,002,177 (GRCm39)	missense	unknown
R5958:Col18a1	UTSW	10	76,932,231 (GRCm39)	missense	probably benign	0.01
R6280:Col18a1	UTSW	10	76,948,323 (GRCm39)	intron	probably benign
R6309:Col18a1	UTSW	10	76,948,576 (GRCm39)	intron	probably benign
R6603:Col18a1	UTSW	10	76,899,811 (GRCm39)	critical splice donor site	probably null
R6608:Col18a1	UTSW	10	76,948,628 (GRCm39)	intron	probably benign
R6805:Col18a1	UTSW	10	76,890,073 (GRCm39)	missense	probably damaging	1.00
R6890:Col18a1	UTSW	10	76,949,318 (GRCm39)	intron	probably benign
R6938:Col18a1	UTSW	10	76,948,333 (GRCm39)	intron	probably benign
R7002:Col18a1	UTSW	10	77,002,177 (GRCm39)	missense	unknown
R7154:Col18a1	UTSW	10	76,908,799 (GRCm39)	missense	probably benign	0.25
R7204:Col18a1	UTSW	10	76,921,110 (GRCm39)	missense	unknown
R7278:Col18a1	UTSW	10	76,932,118 (GRCm39)	missense	unknown
R7442:Col18a1	UTSW	10	76,932,072 (GRCm39)	missense	unknown
R7453:Col18a1	UTSW	10	76,921,044 (GRCm39)	splice site	probably null
R7597:Col18a1	UTSW	10	76,949,137 (GRCm39)	missense	unknown
R7615:Col18a1	UTSW	10	76,902,839 (GRCm39)	missense	probably damaging	1.00
R7671:Col18a1	UTSW	10	76,921,217 (GRCm39)	missense	unknown
R7696:Col18a1	UTSW	10	76,921,106 (GRCm39)	missense	unknown
R7719:Col18a1	UTSW	10	76,913,846 (GRCm39)	missense	probably benign	0.13
R7772:Col18a1	UTSW	10	76,904,220 (GRCm39)	splice site	probably null
R8077:Col18a1	UTSW	10	76,916,685 (GRCm39)	missense	unknown
R8085:Col18a1	UTSW	10	76,924,741 (GRCm39)	missense	unknown
R8097:Col18a1	UTSW	10	76,948,342 (GRCm39)	missense	unknown
R8117:Col18a1	UTSW	10	76,895,808 (GRCm39)	missense	probably benign	0.41
R8130:Col18a1	UTSW	10	76,910,284 (GRCm39)	missense	probably benign	0.03
R8151:Col18a1	UTSW	10	76,948,418 (GRCm39)	missense	unknown
R8379:Col18a1	UTSW	10	76,889,072 (GRCm39)	missense	probably benign	0.08
R8479:Col18a1	UTSW	10	76,916,988 (GRCm39)	missense	unknown
R8523:Col18a1	UTSW	10	76,890,068 (GRCm39)	missense	probably damaging	0.99
R8862:Col18a1	UTSW	10	76,949,044 (GRCm39)	nonsense	probably null
R9109:Col18a1	UTSW	10	76,893,204 (GRCm39)	missense	probably damaging	1.00
R9298:Col18a1	UTSW	10	76,893,204 (GRCm39)	missense	probably damaging	1.00
R9312:Col18a1	UTSW	10	76,894,606 (GRCm39)	missense	probably damaging	0.98
R9366:Col18a1	UTSW	10	76,932,258 (GRCm39)	missense	unknown
R9399:Col18a1	UTSW	10	76,916,584 (GRCm39)	missense	unknown
R9559:Col18a1	UTSW	10	76,913,630 (GRCm39)	missense	probably damaging	1.00
R9649:Col18a1	UTSW	10	76,916,673 (GRCm39)	missense	unknown
R9689:Col18a1	UTSW	10	76,916,578 (GRCm39)	nonsense	probably null
R9719:Col18a1	UTSW	10	76,949,432 (GRCm39)	missense	unknown
Z1176:Col18a1	UTSW	10	76,948,685 (GRCm39)	missense	unknown
Z1176:Col18a1	UTSW	10	76,891,543 (GRCm39)	missense	possibly damaging	0.81
Z1177:Col18a1	UTSW	10	76,948,672 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TCTTCCAAAATTGAGGATGCAGG -3'
(R):5'- TTTCTCCAGCCAGTCAGCAG -3'

Sequencing Primer
(F):5'- ATGCAGGCTATATCCATGGC -3'
(R):5'- GCCAGTCAGCAGCTCAGAAG -3'

Posted On

2016-06-15