Mutagenetix > Incidental Mutation

Incidental Mutation 'R5173:Rnmt'

398953

Institutional Source

Beutler Lab

Gene Symbol

Rnmt

Ensembl Gene

ENSMUSG00000009535

Gene Name

RNA (guanine-7-) methyltransferase

Synonyms

2610002P10Rik

MMRRC Submission

042753-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5173 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

68433426-68457923 bp(+) (GRCm39)

Type of Mutation

utr 3 prime

DNA Base Change (assembly)

C to T at 68454430 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000025427 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000009679] [ENSMUST00000025427]

AlphaFold

Q9D0L8

Predicted Effect

probably benign
Transcript: ENSMUST00000009679

SMART Domains

Protein: ENSMUSP00000009679
Gene: ENSMUSG00000009535

Domain	Start	End	E-Value	Type
Pfam:Pox_MCEL	125	464	7.5e-128	PFAM
Pfam:Methyltransf_31	184	352	1.2e-8	PFAM
Pfam:Methyltransf_11	191	305	3.8e-9	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000025427

SMART Domains

Protein: ENSMUSP00000025427
Gene: ENSMUSG00000009535

Domain	Start	End	E-Value	Type
Pfam:Pox_MCEL	125	317	2.8e-79	PFAM
Pfam:Methyltransf_23	163	349	8.2e-10	PFAM
Pfam:Methyltransf_31	184	375	4.3e-9	PFAM
Pfam:Methyltransf_18	186	308	1.4e-7	PFAM
Pfam:Methyltransf_11	191	305	5.1e-9	PFAM
Pfam:Pox_MCEL	313	409	2.1e-20	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139554

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148937

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

100% (50/50)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	AC	A	11: 9,632,032 (GRCm39)		probably null	Het
Abca6	A	T	11: 110,082,546 (GRCm39)	F1142L	probably benign	Het
Ap1g1	T	A	8: 110,577,764 (GRCm39)		probably null	Het
Apob	T	C	12: 8,058,238 (GRCm39)	V2207A	probably benign	Het
Cenpp	CAAACCTGAAAA	CAAA	13: 49,618,258 (GRCm39)		probably null	Het
Chd3	A	G	11: 69,260,069 (GRCm39)		probably benign	Het
Coch	C	A	12: 51,643,290 (GRCm39)	Y103*	probably null	Het
Cul3	A	T	1: 80,259,133 (GRCm39)	D382E	possibly damaging	Het
Cul5	T	C	9: 53,554,034 (GRCm39)	T291A	probably benign	Het
Dab1	T	C	4: 104,545,645 (GRCm39)		probably null	Het
Dmtf1	A	G	5: 9,190,356 (GRCm39)		probably benign	Het
Dpp4	T	C	2: 62,217,474 (GRCm39)	Y41C	probably damaging	Het
Eif2ak4	A	G	2: 118,238,841 (GRCm39)	I45M	probably damaging	Het
Epn3	T	C	11: 94,386,923 (GRCm39)	K149R	probably damaging	Het
Flnc	A	G	6: 29,455,537 (GRCm39)	E2029G	probably damaging	Het
Gm5799	A	G	14: 43,782,116 (GRCm39)	N96S	probably damaging	Het
Gprc5c	G	T	11: 114,755,093 (GRCm39)	V257L	possibly damaging	Het
Grik5	T	C	7: 24,762,319 (GRCm39)	H224R	possibly damaging	Het
Lpar6	A	T	14: 73,476,537 (GRCm39)	E166V	probably benign	Het
Mical1	T	C	10: 41,360,985 (GRCm39)	L683P	probably damaging	Het
Mis18bp1	T	C	12: 65,196,149 (GRCm39)	I538M	possibly damaging	Het
Mobp	A	G	9: 119,997,311 (GRCm39)	R77G	possibly damaging	Het
Muc21	G	T	17: 35,931,633 (GRCm39)		probably benign	Het
Mylk	A	G	16: 34,797,383 (GRCm39)	H1614R	probably benign	Het
Or2y16	T	C	11: 49,334,713 (GRCm39)	F12L	probably benign	Het
Or4p18	G	A	2: 88,233,266 (GRCm39)	T4I	probably benign	Het
Or7c19	T	C	8: 85,957,205 (GRCm39)	L27P	probably damaging	Het
Osbpl1a	C	T	18: 12,895,697 (GRCm39)	V390I	probably benign	Het
Pcdha7	C	A	18: 37,107,705 (GRCm39)	D243E	probably benign	Het
Pi4ka	A	T	16: 17,168,770 (GRCm39)	N653K	possibly damaging	Het
Plin5	T	G	17: 56,422,548 (GRCm39)		probably null	Het
Plod1	A	G	4: 148,000,758 (GRCm39)		probably benign	Het
Psd3	T	C	8: 68,149,641 (GRCm39)	K372E	probably damaging	Het
Psmd11	C	T	11: 80,351,566 (GRCm39)	T263I	probably benign	Het
Ptprt	A	T	2: 161,769,676 (GRCm39)	N396K	probably benign	Het
Rab39	T	C	9: 53,597,800 (GRCm39)	E155G	probably damaging	Het
Rimbp2	T	C	5: 128,874,712 (GRCm39)	D293G	probably benign	Het
Rnf220	T	C	4: 117,146,471 (GRCm39)		probably benign	Het
Slc10a1	T	A	12: 81,002,802 (GRCm39)	I279F	probably damaging	Het
Sp140l1	G	A	1: 85,078,288 (GRCm39)	R54*	probably null	Het
Taar6	A	G	10: 23,861,250 (GRCm39)	Y99H	probably damaging	Het
Tas2r144	T	A	6: 42,193,048 (GRCm39)	F263I	probably benign	Het
Tex15	T	A	8: 34,061,768 (GRCm39)	N399K	possibly damaging	Het
Tlr9	T	A	9: 106,103,151 (GRCm39)	V814D	possibly damaging	Het
Tmem53	T	A	4: 117,122,908 (GRCm39)		probably benign	Het
Ubap2l	T	C	3: 89,928,337 (GRCm39)	I511V	possibly damaging	Het
Vmn2r60	T	C	7: 41,844,935 (GRCm39)	M766T	probably damaging	Het
Zfp462	T	C	4: 55,011,115 (GRCm39)	V1027A	probably damaging	Het

Other mutations in Rnmt

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02231:Rnmt	APN	18	68,447,152 (GRCm39)	nonsense	probably null
acre	UTSW	18	68,447,105 (GRCm39)	missense	probably damaging	1.00
talenti	UTSW	18	68,452,285 (GRCm39)	missense	probably damaging	0.98
IGL03098:Rnmt	UTSW	18	68,439,073 (GRCm39)	missense	probably damaging	0.98
R0137:Rnmt	UTSW	18	68,446,771 (GRCm39)	missense	probably benign	0.00
R0712:Rnmt	UTSW	18	68,440,859 (GRCm39)	critical splice donor site	probably null
R1493:Rnmt	UTSW	18	68,446,778 (GRCm39)	missense	probably damaging	1.00
R1541:Rnmt	UTSW	18	68,440,853 (GRCm39)	missense	probably damaging	1.00
R1606:Rnmt	UTSW	18	68,444,724 (GRCm39)	missense	possibly damaging	0.83
R2224:Rnmt	UTSW	18	68,438,854 (GRCm39)	start gained	probably benign
R3114:Rnmt	UTSW	18	68,447,079 (GRCm39)	missense	probably benign	0.13
R3115:Rnmt	UTSW	18	68,447,079 (GRCm39)	missense	probably benign	0.13
R4424:Rnmt	UTSW	18	68,444,742 (GRCm39)	missense	probably null	0.07
R4705:Rnmt	UTSW	18	68,447,196 (GRCm39)	missense	probably damaging	1.00
R4722:Rnmt	UTSW	18	68,438,952 (GRCm39)	missense	probably damaging	0.98
R4732:Rnmt	UTSW	18	68,451,031 (GRCm39)	intron	probably benign
R5523:Rnmt	UTSW	18	68,446,773 (GRCm39)	missense	probably benign
R5579:Rnmt	UTSW	18	68,439,186 (GRCm39)	missense	possibly damaging	0.93
R5966:Rnmt	UTSW	18	68,444,689 (GRCm39)	missense	probably benign	0.16
R6322:Rnmt	UTSW	18	68,452,285 (GRCm39)	missense	probably damaging	0.98
R7149:Rnmt	UTSW	18	68,452,222 (GRCm39)	missense	probably damaging	1.00
R7529:Rnmt	UTSW	18	68,444,726 (GRCm39)	missense	probably benign	0.41
R7620:Rnmt	UTSW	18	68,447,105 (GRCm39)	missense	probably damaging	1.00
R8071:Rnmt	UTSW	18	68,440,723 (GRCm39)	missense	probably benign	0.03
R9093:Rnmt	UTSW	18	68,451,146 (GRCm39)	missense	probably benign	0.03
R9436:Rnmt	UTSW	18	68,442,410 (GRCm39)	missense	probably damaging	0.97
Z1088:Rnmt	UTSW	18	68,440,745 (GRCm39)	missense	probably benign	0.14

Predicted Primers

PCR Primer
(F):5'- TGGCTTCTAGTGCTCCTAATGG -3'
(R):5'- AGCTGCTCTGACATGCCTTG -3'

Sequencing Primer
(F):5'- CTAGTGCTCCTAATGGTGTTAAATG -3'
(R):5'- GCTCTGACATGCCTTGCTAGAG -3'

Posted On

2016-07-06