Mutagenetix > Incidental Mutation

Incidental Mutation 'R5619:Or10g9b'

439718

Institutional Source

Beutler Lab

Gene Symbol

Or10g9b

Ensembl Gene

ENSMUSG00000060254

Gene Name

olfactory receptor family 10 subfamily G member 9B

Synonyms

MOR223-2, GA_x6K02T2PVTD-33705428-33704496, Olfr980

MMRRC Submission

043278-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5619 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

39917290-39918325 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 39918039 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Leucine at position 69 (M69L)

Ref Sequence

ENSEMBL: ENSMUSP00000150496 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073932] [ENSMUST00000215523] [ENSMUST00000216463]

AlphaFold

Q8VH08

Predicted Effect

probably benign
Transcript: ENSMUST00000073932
AA Change: M69L

PolyPhen 2 Score 0.074 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000091386
Gene: ENSMUSG00000060254
AA Change: M69L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	304	1.4e-55	PFAM
Pfam:7tm_1	39	287	5.4e-23	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215523

Predicted Effect

probably benign
Transcript: ENSMUST00000216463
AA Change: M69L

PolyPhen 2 Score 0.074 (Sensitivity: 0.93; Specificity: 0.85)

Meta Mutation Damage Score

0.0750

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.4%
20x: 95.7%

Validation Efficiency

99% (80/81)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
Adgre1	T	G	17: 57,727,437 (GRCm39)	L456V	probably benign	Het
Adgrv1	C	T	13: 81,620,619 (GRCm39)	G3943R	probably damaging	Het
Akap9	A	G	5: 4,004,760 (GRCm39)		probably benign	Het
Atp1a2	T	A	1: 172,106,948 (GRCm39)	I791F	probably damaging	Het
BC004004	C	G	17: 29,501,703 (GRCm39)	P81A	probably damaging	Het
Brca2	C	A	5: 150,480,579 (GRCm39)	T2755K	probably damaging	Het
Cacna1c	T	C	6: 118,719,322 (GRCm39)	D215G	probably damaging	Het
Ccdc142	C	T	6: 83,080,603 (GRCm39)	S445F	probably benign	Het
Comt	T	C	16: 18,230,469 (GRCm39)	E80G	probably damaging	Het
Coq7	T	C	7: 118,126,709 (GRCm39)		probably benign	Het
Coro7	C	A	16: 4,494,799 (GRCm39)		probably null	Het
Cyp2c40	A	G	19: 39,792,228 (GRCm39)	S239P	probably damaging	Het
Dnah5	T	C	15: 28,302,581 (GRCm39)	S1613P	probably damaging	Het
Dync2h1	T	C	9: 7,118,885 (GRCm39)	I2193M	probably benign	Het
Eipr1	A	G	12: 28,917,078 (GRCm39)	Y382C	probably damaging	Het
Fastkd2	T	A	1: 63,778,469 (GRCm39)	H447Q	probably benign	Het
Galk2	A	T	2: 125,817,317 (GRCm39)	R369*	probably null	Het
Gli2	G	A	1: 118,764,485 (GRCm39)	A1222V	probably benign	Het
Golim4	T	A	3: 75,813,802 (GRCm39)	K141*	probably null	Het
Gtpbp3	G	T	8: 71,943,692 (GRCm39)		probably benign	Het
Gzmd	C	T	14: 56,367,224 (GRCm39)	A223T	probably benign	Het
Igf2r	T	C	17: 12,958,221 (GRCm39)	R151G	probably damaging	Het
Itga8	T	A	2: 12,270,139 (GRCm39)	R116W	probably damaging	Het
Klhdc1	T	G	12: 69,304,919 (GRCm39)		probably null	Het
Klhl25	T	C	7: 75,516,602 (GRCm39)	Y198H	probably benign	Het
Klhl29	A	T	12: 5,190,587 (GRCm39)	M136K	probably benign	Het
Lipf	A	T	19: 33,944,292 (GRCm39)	Y167F	possibly damaging	Het
Lpar1	T	C	4: 58,487,155 (GRCm39)	K39E	possibly damaging	Het
Mbtd1	T	A	11: 93,820,705 (GRCm39)		probably null	Het
Myo1a	T	A	10: 127,554,413 (GRCm39)	N794K	probably benign	Het
Nmrk1	T	C	19: 18,622,452 (GRCm39)	L177P	possibly damaging	Het
Noxa1	C	T	2: 24,975,988 (GRCm39)	E401K	probably damaging	Het
Or4f53	A	T	2: 111,087,856 (GRCm39)	Y132F	probably damaging	Het
Ostm1	T	A	10: 42,555,325 (GRCm39)	C116S	probably damaging	Het
Pcdhga7	T	C	18: 37,848,800 (GRCm39)	I269T	probably benign	Het
Pfkfb3	T	C	2: 11,489,470 (GRCm39)	K276R	probably benign	Het
Pfkp	A	T	13: 6,648,765 (GRCm39)		probably benign	Het
Pitpnm1	A	G	19: 4,153,270 (GRCm39)	D142G	probably damaging	Het
Pkp3	T	C	7: 140,668,419 (GRCm39)	L556P	probably damaging	Het
Plb1	C	A	5: 32,490,841 (GRCm39)	T1046N	probably damaging	Het
Plxnb2	T	C	15: 89,047,012 (GRCm39)	S770G	possibly damaging	Het
Polk	A	T	13: 96,620,064 (GRCm39)	I733N	probably damaging	Het
Potegl	T	C	2: 23,147,017 (GRCm39)		probably null	Het
Prkg2	C	A	5: 99,136,156 (GRCm39)	C301F	probably damaging	Het
Rabgap1l	T	C	1: 160,066,142 (GRCm39)	T189A	probably benign	Het
Raph1	T	C	1: 60,529,414 (GRCm39)		probably benign	Het
Rbm22	T	A	18: 60,693,899 (GRCm39)	M1K	probably null	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Rnf186	T	A	4: 138,694,715 (GRCm39)	I85N	probably benign	Het
Ryr2	C	A	13: 11,723,088 (GRCm39)	R2517L	probably damaging	Het
Sec63	T	A	10: 42,665,378 (GRCm39)	Y103N	probably damaging	Het
Serpinb3a	G	A	1: 106,974,838 (GRCm39)	P232S	probably damaging	Het
Slco6d1	C	A	1: 98,423,947 (GRCm39)	T533K	probably damaging	Het
Smarcad1	T	A	6: 65,088,865 (GRCm39)	D1000E	probably benign	Het
Spata46	A	T	1: 170,136,490 (GRCm39)	I14F	probably damaging	Het
Speer4b	T	C	5: 27,703,815 (GRCm39)	H106R	possibly damaging	Het
Spint4	T	C	2: 164,542,761 (GRCm39)	L118P	probably benign	Het
Sptbn5	A	G	2: 119,880,613 (GRCm39)		noncoding transcript	Het
Tgfbr3	A	T	5: 107,288,380 (GRCm39)	I427N	probably benign	Het
Thbs2	C	A	17: 14,901,506 (GRCm39)	C491F	probably damaging	Het
Tmem232	T	A	17: 65,793,506 (GRCm39)	E64D	probably benign	Het
Tnpo3	A	T	6: 29,565,197 (GRCm39)	C585*	probably null	Het
Ttc13	A	T	8: 125,406,683 (GRCm39)		probably benign	Het
Tuba8	C	T	6: 121,202,854 (GRCm39)	A389V	probably damaging	Het
Uqcc4	G	A	17: 25,403,963 (GRCm39)	S101N	probably damaging	Het
Usp25	A	G	16: 76,830,833 (GRCm39)	I30V	probably benign	Het
Vmn2r31	T	A	7: 7,387,529 (GRCm39)	K681*	probably null	Het
Vmn2r88	A	G	14: 51,651,367 (GRCm39)	E235G	probably damaging	Het
Vps29	T	A	5: 122,492,511 (GRCm39)		probably benign	Het
Wdr1	A	C	5: 38,686,879 (GRCm39)	V568G	possibly damaging	Het
Zfp64	T	G	2: 168,741,734 (GRCm39)	Q398P	probably damaging	Het
Zfp64	G	T	2: 168,741,735 (GRCm39)	Q398K	probably damaging	Het
Zfp839	T	C	12: 110,830,470 (GRCm39)	Y398H	probably damaging	Het

Other mutations in Or10g9b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02256:Or10g9b	APN	9	39,917,349 (GRCm39)	missense	probably benign
IGL02378:Or10g9b	APN	9	39,917,769 (GRCm39)	missense	probably damaging	1.00
IGL03384:Or10g9b	APN	9	39,917,766 (GRCm39)	missense	probably benign
IGL03402:Or10g9b	APN	9	39,917,802 (GRCm39)	missense	probably benign	0.31
BB010:Or10g9b	UTSW	9	39,918,431 (GRCm39)	start gained	probably benign
PIT4651001:Or10g9b	UTSW	9	39,917,526 (GRCm39)	missense	probably damaging	0.97
R0013:Or10g9b	UTSW	9	39,917,651 (GRCm39)	missense	probably damaging	1.00
R1146:Or10g9b	UTSW	9	39,917,390 (GRCm39)	missense	possibly damaging	0.95
R1146:Or10g9b	UTSW	9	39,917,390 (GRCm39)	missense	possibly damaging	0.95
R4541:Or10g9b	UTSW	9	39,917,589 (GRCm39)	missense	possibly damaging	0.95
R4562:Or10g9b	UTSW	9	39,917,577 (GRCm39)	missense	probably damaging	0.99
R4731:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4732:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4733:Or10g9b	UTSW	9	39,917,564 (GRCm39)	missense	probably damaging	1.00
R4825:Or10g9b	UTSW	9	39,918,038 (GRCm39)	missense	possibly damaging	0.72
R5770:Or10g9b	UTSW	9	39,917,634 (GRCm39)	missense	probably benign	0.01
R5791:Or10g9b	UTSW	9	39,918,030 (GRCm39)	missense	probably damaging	1.00
R6813:Or10g9b	UTSW	9	39,917,753 (GRCm39)	missense	probably benign
R6819:Or10g9b	UTSW	9	39,917,844 (GRCm39)	missense	probably benign	0.00
R6970:Or10g9b	UTSW	9	39,918,009 (GRCm39)	missense	probably benign	0.00
R7490:Or10g9b	UTSW	9	39,917,720 (GRCm39)	missense	probably damaging	1.00
R7511:Or10g9b	UTSW	9	39,918,229 (GRCm39)	missense	possibly damaging	0.63
R7933:Or10g9b	UTSW	9	39,918,431 (GRCm39)	start gained	probably benign
R8243:Or10g9b	UTSW	9	39,917,484 (GRCm39)	missense	probably benign	0.19
Z1088:Or10g9b	UTSW	9	39,917,892 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- ACTCATCATGCTGCTGTACC -3'
(R):5'- ATGGAAGTGTAGTGACCACG -3'

Sequencing Primer
(F):5'- CATCATGCTGCTGTACCTGAGTG -3'
(R):5'- GAAGTGTAGTGACCACGTTCTTCC -3'

Posted On

2016-11-08