Incidental Mutation 'R6674:Zfp981'
| ID |
527212 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp981
|
| Ensembl Gene |
ENSMUSG00000056300 |
| Gene Name |
zinc finger protein 981 |
| Synonyms |
Gm13247 |
| MMRRC Submission |
044794-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.094)
|
| Stock # |
R6674 (G1)
|
| Quality Score |
98.0078 |
|
Status
|
Not validated
|
| Chromosome |
4 |
| Chromosomal Location |
146586484-146623852 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 146619950 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Glycine
at position 35
(R35G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000115886
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000105735]
[ENSMUST00000140089]
[ENSMUST00000179175]
|
| AlphaFold |
A2A8V7 |
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000105735
AA Change: R35G
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000101361
Gene: ENSMUSG00000056300
AA Change: R35G
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
13 |
73 |
2.34e-15 |
SMART |
|
ZnF_C2H2
|
239 |
261 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
267 |
289 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
295 |
317 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
323 |
345 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
351 |
373 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
379 |
401 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
407 |
429 |
3.11e-2 |
SMART |
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000140089
AA Change: R35G
PolyPhen 2
Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)
|
| SMART Domains |
Protein: ENSMUSP00000115886
Gene: ENSMUSG00000056300
AA Change: R35G
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
13 |
73 |
2.34e-15 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000145269
|
| Predicted Effect |
probably damaging
Transcript: ENSMUST00000179175
AA Change: R35G
PolyPhen 2
Score 0.966 (Sensitivity: 0.77; Specificity: 0.95)
|
| SMART Domains |
Protein: ENSMUSP00000136739
Gene: ENSMUSG00000056300
AA Change: R35G
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
13 |
73 |
2.34e-15 |
SMART |
|
ZnF_C2H2
|
239 |
261 |
7.9e-4 |
SMART |
|
ZnF_C2H2
|
267 |
289 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
295 |
317 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
323 |
345 |
1.36e-2 |
SMART |
|
ZnF_C2H2
|
351 |
373 |
6.32e-3 |
SMART |
|
ZnF_C2H2
|
379 |
401 |
8.6e-5 |
SMART |
|
ZnF_C2H2
|
407 |
429 |
3.11e-2 |
SMART |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000181199
|
| Coding Region Coverage |
- 1x: 99.9%
- 3x: 99.6%
- 10x: 98.3%
- 20x: 95.4%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 25 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Armh3 |
T |
C |
19: 45,963,437 (GRCm39) |
E92G |
probably benign |
Het |
| Cluh |
A |
G |
11: 74,557,053 (GRCm39) |
H1005R |
probably damaging |
Het |
| Coro2b |
C |
T |
9: 62,339,709 (GRCm39) |
R13H |
probably damaging |
Het |
| Depdc1a |
T |
A |
3: 159,232,344 (GRCm39) |
N698K |
probably benign |
Het |
| Dusp12 |
G |
A |
1: 170,707,317 (GRCm39) |
T257I |
probably benign |
Het |
| Esf1 |
T |
A |
2: 139,962,726 (GRCm39) |
K782* |
probably null |
Het |
| Extl1 |
T |
C |
4: 134,085,438 (GRCm39) |
T560A |
probably damaging |
Het |
| Gpr179 |
C |
A |
11: 97,238,231 (GRCm39) |
|
probably null |
Het |
| Krt90 |
G |
A |
15: 101,465,761 (GRCm39) |
L287F |
probably damaging |
Het |
| Mertk |
T |
C |
2: 128,571,277 (GRCm39) |
V77A |
probably benign |
Het |
| Nr4a2 |
C |
T |
2: 57,002,436 (GRCm39) |
A6T |
probably damaging |
Het |
| Pdzd8 |
G |
A |
19: 59,289,801 (GRCm39) |
P533L |
probably damaging |
Het |
| Plxnb1 |
G |
A |
9: 108,937,214 (GRCm39) |
G1132R |
probably benign |
Het |
| Ppfia2 |
A |
G |
10: 106,763,633 (GRCm39) |
I1209V |
probably benign |
Het |
| Slc26a9 |
A |
T |
1: 131,692,756 (GRCm39) |
Q696L |
probably benign |
Het |
| Sphkap |
A |
T |
1: 83,255,555 (GRCm39) |
S444R |
probably benign |
Het |
| Tmem144 |
A |
G |
3: 79,746,490 (GRCm39) |
F22L |
possibly damaging |
Het |
| Tomm20l |
A |
G |
12: 71,158,307 (GRCm39) |
D30G |
probably damaging |
Het |
| Trpc2 |
A |
G |
7: 101,745,264 (GRCm39) |
T827A |
probably benign |
Het |
| Unc50 |
T |
C |
1: 37,476,539 (GRCm39) |
V208A |
probably benign |
Het |
| Vmn1r125 |
T |
C |
7: 21,006,638 (GRCm39) |
S179P |
probably damaging |
Het |
| Vmn1r225 |
A |
C |
17: 20,723,377 (GRCm39) |
I273L |
probably benign |
Het |
| Vmn2r117 |
G |
A |
17: 23,679,023 (GRCm39) |
Q734* |
probably null |
Het |
| Vmn2r88 |
A |
G |
14: 51,651,795 (GRCm39) |
I378V |
probably benign |
Het |
| Zfp507 |
C |
T |
7: 35,494,159 (GRCm39) |
V295I |
probably damaging |
Het |
|
| Other mutations in Zfp981 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02647:Zfp981
|
APN |
4 |
146,621,709 (GRCm39) |
nonsense |
probably null |
|
|
R0003:Zfp981
|
UTSW |
4 |
146,622,217 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1172:Zfp981
|
UTSW |
4 |
146,622,221 (GRCm39) |
missense |
probably benign |
|
|
R2989:Zfp981
|
UTSW |
4 |
146,622,347 (GRCm39) |
missense |
probably benign |
0.40 |
|
R4158:Zfp981
|
UTSW |
4 |
146,622,339 (GRCm39) |
missense |
probably benign |
0.07 |
|
R4158:Zfp981
|
UTSW |
4 |
146,622,080 (GRCm39) |
missense |
probably benign |
|
|
R4778:Zfp981
|
UTSW |
4 |
146,622,112 (GRCm39) |
missense |
probably benign |
|
|
R5148:Zfp981
|
UTSW |
4 |
146,621,357 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R5352:Zfp981
|
UTSW |
4 |
146,621,462 (GRCm39) |
missense |
probably benign |
0.29 |
|
R6252:Zfp981
|
UTSW |
4 |
146,621,970 (GRCm39) |
missense |
probably benign |
0.22 |
|
R6765:Zfp981
|
UTSW |
4 |
146,622,363 (GRCm39) |
missense |
probably benign |
0.34 |
|
R7288:Zfp981
|
UTSW |
4 |
146,622,100 (GRCm39) |
missense |
probably benign |
0.32 |
|
R7816:Zfp981
|
UTSW |
4 |
146,622,100 (GRCm39) |
missense |
probably benign |
0.32 |
|
R7835:Zfp981
|
UTSW |
4 |
146,622,333 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8020:Zfp981
|
UTSW |
4 |
146,621,825 (GRCm39) |
missense |
possibly damaging |
0.91 |
|
R8797:Zfp981
|
UTSW |
4 |
146,619,906 (GRCm39) |
nonsense |
probably null |
|
|
R8805:Zfp981
|
UTSW |
4 |
146,622,410 (GRCm39) |
missense |
possibly damaging |
0.86 |
|
R9145:Zfp981
|
UTSW |
4 |
146,622,410 (GRCm39) |
missense |
possibly damaging |
0.72 |
|
R9324:Zfp981
|
UTSW |
4 |
146,619,884 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R9471:Zfp981
|
UTSW |
4 |
146,622,080 (GRCm39) |
missense |
probably benign |
|
|
Z1176:Zfp981
|
UTSW |
4 |
146,621,547 (GRCm39) |
missense |
probably damaging |
0.96 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCAGTGCGCTAAATCATTCTATAG -3'
(R):5'- TCAGGTTGTCCCAGAACAAAAC -3'
Sequencing Primer
(F):5'- GGTAACGCAATAATTCATGTCTTCAG -3'
(R):5'- GGTTGTCCCAGAACAAAACACAGAG -3'
|
| Posted On |
2018-07-23 |
Incidental Mutation