Mutagenetix > Incidental Mutation

Incidental Mutation 'R6849:Or13g1'

534910

Institutional Source

Beutler Lab

Gene Symbol

Or13g1

Ensembl Gene

ENSMUSG00000054054

Gene Name

olfactory receptor family 13 subfamily G member 1

Synonyms

MOR251-4P, GA_x6K02T2NHDJ-9801340-9802266, Olfr309

MMRRC Submission

044953-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R6849 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

85955393-85956319 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85956248 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Methionine at position 24 (I24M)

Ref Sequence

ENSEMBL: ENSMUSP00000134377 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055690] [ENSMUST00000174158]

AlphaFold

Q7TS01

Predicted Effect

possibly damaging
Transcript: ENSMUST00000055690
AA Change: I24M

PolyPhen 2 Score 0.538 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000061929
Gene: ENSMUSG00000054054
AA Change: I24M

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	303	4.5e-11	PFAM
Pfam:7tm_1	39	288	1.5e-26	PFAM
Pfam:7tm_4	137	281	7.9e-44	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000174158
AA Change: I24M

PolyPhen 2 Score 0.538 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000134377
Gene: ENSMUSG00000054054
AA Change: I24M

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	305	1.6e-55	PFAM
Pfam:7TM_GPCR_Srsx	33	303	4.5e-11	PFAM
Pfam:7tm_1	39	288	2.4e-18	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.5%

Validation Efficiency

100% (55/55)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310022B05Rik	G	T	8: 125,366,261 (GRCm39)	Q161K	probably damaging	Het
4930578I06Rik	A	T	14: 64,223,687 (GRCm39)	W30R	probably damaging	Het
4930578I06Rik	G	T	14: 64,223,688 (GRCm39)	N29K	probably benign	Het
Aldh6a1	A	G	12: 84,490,561 (GRCm39)	V18A	probably benign	Het
Apoa5	A	G	9: 46,181,298 (GRCm39)	K125E	probably benign	Het
Atosa	A	C	9: 74,916,594 (GRCm39)	N398H	probably damaging	Het
Bphl	T	A	13: 34,234,252 (GRCm39)		probably null	Het
C2cd3	T	A	7: 100,056,134 (GRCm39)	V514E	probably damaging	Het
Cacna1s	G	A	1: 136,020,432 (GRCm39)	R823Q	probably benign	Het
Cep192	A	G	18: 67,945,506 (GRCm39)	D202G	probably benign	Het
Chd5	A	T	4: 152,462,995 (GRCm39)	N1420Y	probably damaging	Het
Cnot10	A	T	9: 114,461,004 (GRCm39)	D55E	probably benign	Het
Cntn1	T	A	15: 92,203,127 (GRCm39)	I803N	probably damaging	Het
Col7a1	G	T	9: 108,804,121 (GRCm39)	V2217L	unknown	Het
Cpne9	T	G	6: 113,279,079 (GRCm39)	V491G	probably damaging	Het
Cracd	C	A	5: 77,004,857 (GRCm39)	A406E	unknown	Het
Cracd	C	T	5: 77,005,004 (GRCm39)	A455V	unknown	Het
Csnk2a1	C	A	2: 152,092,484 (GRCm39)	H18Q	probably benign	Het
D130040H23Rik	T	A	8: 69,755,303 (GRCm39)	Y253*	probably null	Het
Dnah14	A	T	1: 181,636,510 (GRCm39)	M4321L	probably benign	Het
Dnah5	A	G	15: 28,278,770 (GRCm39)	T1122A	probably benign	Het
Eif4g1	A	G	16: 20,499,495 (GRCm39)	I515V	probably benign	Het
Fbn1	T	C	2: 125,163,611 (GRCm39)	K2082E	possibly damaging	Het
Fstl1	A	G	16: 37,641,521 (GRCm39)	K99R	probably benign	Het
Gar1	T	C	3: 129,623,038 (GRCm39)	N117S	probably damaging	Het
Gm3238	A	T	10: 77,606,744 (GRCm39)		probably benign	Het
Gm32742	C	T	9: 51,050,014 (GRCm39)	M1528I	probably benign	Het
H2-T3	T	C	17: 36,500,697 (GRCm39)	I49V	probably benign	Het
Hyou1	A	G	9: 44,298,561 (GRCm39)	I581V	probably damaging	Het
Itk	T	C	11: 46,222,762 (GRCm39)	N563S	probably damaging	Het
Lingo1	A	G	9: 56,526,900 (GRCm39)	L563P	probably damaging	Het
Lipc	A	G	9: 70,726,129 (GRCm39)		probably null	Het
Map4k3	A	C	17: 80,937,842 (GRCm39)		probably null	Het
Mctp2	C	T	7: 71,861,466 (GRCm39)	C393Y	probably damaging	Het
Mei1	T	A	15: 81,964,146 (GRCm39)	L229M	possibly damaging	Het
Or9i2	G	T	19: 13,816,203 (GRCm39)	C111*	probably null	Het
Pcnx2	A	G	8: 126,587,949 (GRCm39)	V833A	probably damaging	Het
Pde8b	T	C	13: 95,184,307 (GRCm39)	N388D	possibly damaging	Het
Pi4ka	G	A	16: 17,121,285 (GRCm39)	A1197V	possibly damaging	Het
Psd	A	G	19: 46,306,185 (GRCm39)	Y36H	probably damaging	Het
Scn8a	T	A	15: 100,853,468 (GRCm39)		probably null	Het
Shisa6	A	G	11: 66,416,327 (GRCm39)	V155A	probably benign	Het
Slc45a3	T	A	1: 131,905,702 (GRCm39)	C242S	probably damaging	Het
Sorbs1	G	C	19: 40,365,244 (GRCm39)	R180G	probably benign	Het
Tmc3	T	A	7: 83,235,565 (GRCm39)	I54K	probably damaging	Het
Tmprss11g	T	A	5: 86,644,491 (GRCm39)	I118F	probably benign	Het
Ttn	T	A	2: 76,744,687 (GRCm39)	D5454V	possibly damaging	Het
Ube2f	A	G	1: 91,181,935 (GRCm39)		probably null	Het
Ubr7	T	C	12: 102,724,342 (GRCm39)	S19P	probably damaging	Het
Vav3	T	C	3: 109,428,782 (GRCm39)	V371A	probably damaging	Het
Vmn2r28	A	G	7: 5,483,806 (GRCm39)	V798A	probably damaging	Het
Vmn2r95	T	G	17: 18,664,181 (GRCm39)	C467G	probably damaging	Het
Vmn2r95	G	T	17: 18,664,182 (GRCm39)	C467F	probably damaging	Het
Vps13b	A	G	15: 35,905,455 (GRCm39)	D3325G	probably damaging	Het
Wnk2	T	C	13: 49,220,834 (GRCm39)	T1158A	probably damaging	Het
Zfp616	T	A	11: 73,976,276 (GRCm39)	N848K	possibly damaging	Het

Other mutations in Or13g1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00886:Or13g1	APN	7	85,956,259 (GRCm39)	missense	probably damaging	1.00
R0370:Or13g1	UTSW	7	85,956,057 (GRCm39)	missense	probably benign	0.42
R1869:Or13g1	UTSW	7	85,955,875 (GRCm39)	missense	possibly damaging	0.96
R2047:Or13g1	UTSW	7	85,956,012 (GRCm39)	missense	probably damaging	1.00
R3939:Or13g1	UTSW	7	85,955,437 (GRCm39)	missense	probably benign	0.00
R4562:Or13g1	UTSW	7	85,956,360 (GRCm39)	start gained	probably benign
R4640:Or13g1	UTSW	7	85,956,274 (GRCm39)	missense	probably benign	0.01
R4811:Or13g1	UTSW	7	85,956,166 (GRCm39)	missense	probably benign	0.41
R4867:Or13g1	UTSW	7	85,955,491 (GRCm39)	missense	probably benign	0.00
R4954:Or13g1	UTSW	7	85,955,809 (GRCm39)	missense	probably benign	0.01
R5784:Or13g1	UTSW	7	85,955,743 (GRCm39)	missense	probably damaging	1.00
R6043:Or13g1	UTSW	7	85,955,547 (GRCm39)	missense	probably damaging	0.97
R6498:Or13g1	UTSW	7	85,956,226 (GRCm39)	missense	probably benign
R7238:Or13g1	UTSW	7	85,955,799 (GRCm39)	missense	probably damaging	1.00
R7811:Or13g1	UTSW	7	85,955,554 (GRCm39)	missense	probably damaging	1.00
R7917:Or13g1	UTSW	7	85,955,686 (GRCm39)	missense	probably damaging	1.00
R8010:Or13g1	UTSW	7	85,956,260 (GRCm39)	missense	probably benign	0.24
R8271:Or13g1	UTSW	7	85,955,962 (GRCm39)	missense	probably benign	0.13
R9017:Or13g1	UTSW	7	85,955,957 (GRCm39)	nonsense	probably null
R9150:Or13g1	UTSW	7	85,955,942 (GRCm39)	missense	probably damaging	0.96
T0975:Or13g1	UTSW	7	85,955,492 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- AAGAGCTGGGACATGCAACC -3'
(R):5'- CCCATCATAGAGAAACTTATGCTAC -3'

Sequencing Primer
(F):5'- TGGGACATGCAACCCCCATATG -3'
(R):5'- GTCTAACCACTCATGCATG -3'

Posted On

2018-09-12