Mutagenetix > Incidental Mutation

Incidental Mutation 'R8172:Lyg2'

634172

Institutional Source

Beutler Lab

Gene Symbol

Lyg2

Ensembl Gene

ENSMUSG00000061584

Gene Name

lysozyme G-like 2

Synonyms

LOC332427

MMRRC Submission

067598-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.058) question?

Stock #

R8172 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37945004-37955574 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 37946748 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 178 (T178A)

Ref Sequence

ENSEMBL: ENSMUSP00000077422 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000078307]

AlphaFold

Q3V1I0

Predicted Effect

probably benign
Transcript: ENSMUST00000078307
AA Change: T178A

PolyPhen 2 Score 0.013 (Sensitivity: 0.96; Specificity: 0.78)

SMART Domains

Protein: ENSMUSP00000077422
Gene: ENSMUSG00000061584
AA Change: T178A

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
SCOP:d153l__	39	213	5e-45	SMART
PDB:1LSP\|A	40	213	2e-56	PDB

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.3%
20x: 97.6%

Validation Efficiency

100% (53/53)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene contains a SLT domain, a protein domain present in bacterial lytic transglycosylase (SLT) and in eukaryotic lysozymes (GEWL). SLT domain catalyzes the cleavage of the beta-1,4-glycosidic bond between N-acetylmuramic acid (MurNAc) and N-acetyglucosamine (GlcNAc). [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy5	T	A	16: 34,977,427 (GRCm39)	L320Q	probably damaging	Het
Adgre4	A	T	17: 56,104,769 (GRCm39)	L278F	probably benign	Het
Agfg2	C	A	5: 137,665,431 (GRCm39)	R108L	probably damaging	Het
Arap2	A	T	5: 62,779,324 (GRCm39)		probably null	Het
Ascl2	G	T	7: 142,522,336 (GRCm39)	N37K	possibly damaging	Het
Baiap3	C	A	17: 25,463,096 (GRCm39)	D1043Y	probably damaging	Het
Cby2	T	A	14: 75,829,241 (GRCm39)		probably null	Het
Ccdc18	T	C	5: 108,311,640 (GRCm39)		probably null	Het
Cemip	T	A	7: 83,646,433 (GRCm39)	D205V	probably damaging	Het
Cenpn	G	A	8: 117,658,333 (GRCm39)	G93D	probably benign	Het
Clpsl2	T	A	17: 28,768,556 (GRCm39)	S23R	possibly damaging	Het
Cnot3	T	A	7: 3,661,724 (GRCm39)	I672N	possibly damaging	Het
Crygs	A	G	16: 22,625,292 (GRCm39)	Y50H	probably damaging	Het
Cyp4x1	T	C	4: 114,968,874 (GRCm39)	T403A	possibly damaging	Het
Dnajc28	G	A	16: 91,413,795 (GRCm39)	R150*	probably null	Het
Fam184b	C	T	5: 45,741,709 (GRCm39)	G174D	possibly damaging	Het
Fat2	T	A	11: 55,178,638 (GRCm39)	D1474V	probably damaging	Het
Flg2	T	A	3: 93,108,468 (GRCm39)	D165E	possibly damaging	Het
Fpr-rs7	A	T	17: 20,334,443 (GRCm39)	F16I	probably benign	Het
Gpr176	G	A	2: 118,114,615 (GRCm39)	T65I	probably damaging	Het
H2-Q10	C	T	17: 35,781,996 (GRCm39)	T206I	probably null	Het
Hadha	C	G	5: 30,350,285 (GRCm39)	A88P	probably damaging	Het
Hlcs	A	T	16: 94,068,485 (GRCm39)	L245Q	probably damaging	Het
Hnrnph1	T	C	11: 50,270,732 (GRCm39)	V113A	probably damaging	Het
Hsd3b7	G	A	7: 127,401,546 (GRCm39)	V224M	probably damaging	Het
Igha	T	C	12: 113,223,592 (GRCm39)	D88G		Het
Iqca1l	T	C	5: 24,748,608 (GRCm39)	M803V	probably benign	Het
Kdm7a	T	C	6: 39,125,965 (GRCm39)	K610R	probably benign	Het
Krt87	C	T	15: 101,383,284 (GRCm39)	C474Y	probably benign	Het
Lrrc74a	T	A	12: 86,788,530 (GRCm39)	L170H	probably damaging	Het
Map2k2	T	A	10: 80,959,442 (GRCm39)		probably null	Het
Mast4	C	T	13: 103,089,633 (GRCm39)		probably null	Het
Mtmr14	T	A	6: 113,216,529 (GRCm39)	D8E	probably benign	Het
Neu2	C	T	1: 87,524,633 (GRCm39)	P206L	probably damaging	Het
Oga	C	T	19: 45,765,339 (GRCm39)	R156H	probably damaging	Het
Or10al6	A	G	17: 38,083,326 (GRCm39)	T261A	probably benign	Het
Or4c105	A	T	2: 88,647,986 (GRCm39)	Q157L	probably damaging	Het
Poc1b	C	A	10: 98,980,338 (GRCm39)		probably null	Het
Proser1	T	A	3: 53,386,272 (GRCm39)	V718E	possibly damaging	Het
Ptgr2	T	A	12: 84,360,783 (GRCm39)	L351Q	possibly damaging	Het
Ptprf	A	G	4: 118,068,275 (GRCm39)	Y1754H	probably benign	Het
Scgb2b3	T	C	7: 31,058,476 (GRCm39)	K109R	possibly damaging	Het
Scn2a	A	T	2: 65,520,672 (GRCm39)	H556L	probably benign	Het
Scn7a	A	T	2: 66,506,191 (GRCm39)	M1566K	possibly damaging	Het
Slco5a1	A	T	1: 13,060,490 (GRCm39)	L77*	probably null	Het
Stk31	T	C	6: 49,394,261 (GRCm39)	F208L	possibly damaging	Het
Tbcd	C	T	11: 121,384,711 (GRCm39)	T315M	probably benign	Het
Tbx4	A	G	11: 85,801,933 (GRCm39)	I189V	probably benign	Het
Tia1	T	A	6: 86,404,682 (GRCm39)	Y306N	probably benign	Het
Ttc21b	T	A	2: 66,082,500 (GRCm39)	Y33F	probably benign	Het
Usp47	T	A	7: 111,687,133 (GRCm39)	L677*	probably null	Het

Other mutations in Lyg2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02834:Lyg2	APN	1	37,949,048 (GRCm39)	missense	probably damaging	1.00
IGL03079:Lyg2	APN	1	37,946,727 (GRCm39)	missense	possibly damaging	0.90
IGL03123:Lyg2	APN	1	37,954,845 (GRCm39)	utr 5 prime	probably benign
R0543:Lyg2	UTSW	1	37,950,188 (GRCm39)	missense	possibly damaging	0.94
R2250:Lyg2	UTSW	1	37,954,816 (GRCm39)	missense	probably benign	0.25
R2258:Lyg2	UTSW	1	37,948,077 (GRCm39)	missense	probably benign	0.00
R3884:Lyg2	UTSW	1	37,949,150 (GRCm39)	missense	probably damaging	1.00
R4807:Lyg2	UTSW	1	37,950,148 (GRCm39)	missense	possibly damaging	0.54
R5991:Lyg2	UTSW	1	37,954,800 (GRCm39)	critical splice donor site	probably null
R6328:Lyg2	UTSW	1	37,950,194 (GRCm39)	missense	probably benign	0.33
R7439:Lyg2	UTSW	1	37,950,218 (GRCm39)	missense	possibly damaging	0.46
R8812:Lyg2	UTSW	1	37,949,054 (GRCm39)	missense	probably damaging	1.00
Z1176:Lyg2	UTSW	1	37,950,208 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CTCCAATTGAATGCAGTGGG -3'
(R):5'- ACTCAGTGAGGAAACTTTCTGAC -3'

Sequencing Primer
(F):5'- GCAGTGGGCTAATATTAAACCTTGG -3'
(R):5'- GGAAACTTTCTGACATAATAGGTGC -3'

Posted On

2020-07-13