Mutagenetix > Incidental Mutation

Incidental Mutation 'R9250:Zfp759'

701504

Institutional Source

Beutler Lab

Gene Symbol

Zfp759

Ensembl Gene

ENSMUSG00000057396

Gene Name

zinc finger protein 759

Synonyms

Rslcan-8

MMRRC Submission

068989-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R9250 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

67273040-67290468 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 67288461 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Glycine at position 671 (C671G)

Ref Sequence

ENSEMBL: ENSMUSP00000049650 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000052716] [ENSMUST00000224346]

AlphaFold

Q7M6X3

Predicted Effect

probably damaging
Transcript: ENSMUST00000052716
AA Change: C671G

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000049650
Gene: ENSMUSG00000057396
AA Change: C671G

Domain	Start	End	E-Value	Type
KRAB	5	65	1.6e-22	SMART
ZnF_C2H2	106	128	5.54e1	SMART
ZnF_C2H2	162	184	3.83e-2	SMART
ZnF_C2H2	190	212	1.82e-3	SMART
ZnF_C2H2	218	240	1.64e-1	SMART
ZnF_C2H2	246	268	1.67e-2	SMART
ZnF_C2H2	274	296	1.95e-3	SMART
ZnF_C2H2	302	324	1.84e-4	SMART
ZnF_C2H2	330	352	7.78e-3	SMART
ZnF_C2H2	358	380	1.6e-4	SMART
ZnF_C2H2	386	408	1.67e-2	SMART
ZnF_C2H2	414	436	4.87e-4	SMART
ZnF_C2H2	442	464	3.39e-3	SMART
ZnF_C2H2	498	520	2.57e-3	SMART
ZnF_C2H2	526	548	8.47e-4	SMART
ZnF_C2H2	554	576	2.02e-1	SMART
ZnF_C2H2	582	604	2.53e-2	SMART
ZnF_C2H2	610	632	4.79e-3	SMART
ZnF_C2H2	638	660	1.84e-4	SMART
ZnF_C2H2	666	688	1.36e-2	SMART
ZnF_C2H2	694	716	4.17e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000224346

Meta Mutation Damage Score

0.3643

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.5%
20x: 98.0%

Validation Efficiency

98% (55/56)

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	C	G	3: 137,772,389 (GRCm39)	A526G	probably damaging	Het
AAdacl4fm3	A	T	4: 144,442,011 (GRCm39)	I80K	probably benign	Het
Ablim3	A	G	18: 61,944,501 (GRCm39)	L526P	probably damaging	Het
Aknad1	T	C	3: 108,664,143 (GRCm39)	S369P	probably damaging	Het
Aldh8a1	T	C	10: 21,258,259 (GRCm39)	S119P	probably damaging	Het
Brpf3	T	C	17: 29,054,788 (GRCm39)	I1110T	probably damaging	Het
Cdk12	T	C	11: 98,101,398 (GRCm39)	S419P	probably benign	Het
Cmklr1	G	C	5: 113,752,043 (GRCm39)	H319Q	probably benign	Het
Crim1	C	T	17: 78,677,471 (GRCm39)	H891Y	probably benign	Het
Dapp1	T	C	3: 137,638,919 (GRCm39)	E269G	probably benign	Het
Ddt	T	C	10: 75,609,063 (GRCm39)	D32G	probably benign	Het
Dlg5	T	C	14: 24,240,543 (GRCm39)	D236G	probably benign	Het
Edem1	C	A	6: 108,805,850 (GRCm39)	D50E	probably benign	Het
Exoc5	A	T	14: 49,256,915 (GRCm39)	N490K	probably damaging	Het
Fam117a	C	A	11: 95,228,071 (GRCm39)	D60E	possibly damaging	Het
Fam186a	A	G	15: 99,845,330 (GRCm39)	S305P	unknown	Het
Fbxw21	A	G	9: 108,972,846 (GRCm39)	F363L	probably benign	Het
Fhl2	A	G	1: 43,167,422 (GRCm39)	C224R	probably damaging	Het
Gad1	T	A	2: 70,409,543 (GRCm39)	N211K	probably damaging	Het
Igdcc4	A	G	9: 65,038,932 (GRCm39)	T906A	possibly damaging	Het
Igkv3-5	T	C	6: 70,640,758 (GRCm39)	S80P	probably benign	Het
Igkv4-90	C	A	6: 68,784,331 (GRCm39)	G62*	probably null	Het
Il31ra	A	T	13: 112,669,508 (GRCm39)	Y386N	probably damaging	Het
Kif13a	G	T	13: 46,928,909 (GRCm39)	H1159N	probably damaging	Het
Kmt2a	G	A	9: 44,759,683 (GRCm39)	S722F	possibly damaging	Het
Mipep	T	C	14: 61,028,358 (GRCm39)	Y157H	probably damaging	Het
Mipol1	A	G	12: 57,461,169 (GRCm39)	E293G	probably damaging	Het
Mmp7	A	T	9: 7,697,885 (GRCm39)		probably benign	Het
Mr1	A	G	1: 155,013,325 (GRCm39)	L84P	probably damaging	Het
Neb	A	G	2: 52,168,913 (GRCm39)	V1874A	possibly damaging	Het
Nefm	T	C	14: 68,358,570 (GRCm39)		probably benign	Het
Nek1	T	C	8: 61,465,151 (GRCm39)	M80T	probably damaging	Het
Nubp2	A	G	17: 25,103,373 (GRCm39)		probably null	Het
Or10ab4	C	T	7: 107,655,014 (GRCm39)	T275M	probably damaging	Het
Or5w11	T	C	2: 87,459,278 (GRCm39)	I41T	probably benign	Het
Or8b101	T	A	9: 38,020,718 (GRCm39)	C240*	probably null	Het
Ovch2	C	A	7: 107,392,542 (GRCm39)	R267L	probably damaging	Het
Pole	A	G	5: 110,447,687 (GRCm39)	E741G	possibly damaging	Het
Ptprh	T	A	7: 4,576,289 (GRCm39)	T324S	probably benign	Het
Pxk	T	A	14: 8,144,123 (GRCm38)	H290Q	probably damaging	Het
Rab11fip3	G	T	17: 26,237,219 (GRCm39)	T5N	unknown	Het
Rp9	G	A	9: 22,365,086 (GRCm39)	Q124*	probably null	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,121 (GRCm39)		probably benign	Het
Runx2	A	G	17: 45,125,459 (GRCm39)	L19P	probably benign	Het
Sf1	C	T	19: 6,424,764 (GRCm39)	P473L	unknown	Het
Slc9a2	C	T	1: 40,806,987 (GRCm39)	T758I	probably benign	Het
Spef1l	C	A	7: 139,556,395 (GRCm39)	K197N	probably benign	Het
Tas2r105	T	G	6: 131,663,951 (GRCm39)	H159P	probably benign	Het
Tas2r120	T	A	6: 132,633,954 (GRCm39)	M12K	probably benign	Het
Thoc2l	A	G	5: 104,667,320 (GRCm39)	Y614C	probably benign	Het
Trpv4	T	C	5: 114,764,941 (GRCm39)	Y691C	probably damaging	Het
Unc93a	A	G	17: 13,334,488 (GRCm39)	V337A	probably benign	Het
Usp17le	T	G	7: 104,418,839 (GRCm39)	Q101P	probably damaging	Het
Usp3	A	T	9: 66,449,793 (GRCm39)		probably null	Het
Zfp445	T	C	9: 122,681,092 (GRCm39)	S950G	possibly damaging	Het
Zfp644	T	G	5: 106,784,699 (GRCm39)	D616A	probably damaging	Het
Zgrf1	G	T	3: 127,379,797 (GRCm39)	C1111F	probably damaging	Het
Zmym2	T	A	14: 57,148,732 (GRCm39)	S349T	probably damaging	Het

Other mutations in Zfp759

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01509:Zfp759	APN	13	67,287,658 (GRCm39)	missense	probably benign	0.25
IGL03131:Zfp759	APN	13	67,286,728 (GRCm39)	missense	probably damaging	1.00
IGL03218:Zfp759	APN	13	67,287,480 (GRCm39)	missense	probably benign	0.00
R0243:Zfp759	UTSW	13	67,286,877 (GRCm39)	missense	possibly damaging	0.66
R0319:Zfp759	UTSW	13	67,288,356 (GRCm39)	missense	probably benign	0.00
R0520:Zfp759	UTSW	13	67,285,419 (GRCm39)	missense	probably benign	0.29
R0961:Zfp759	UTSW	13	67,287,927 (GRCm39)	missense	probably benign	0.32
R1435:Zfp759	UTSW	13	67,286,830 (GRCm39)	missense	possibly damaging	0.73
R1649:Zfp759	UTSW	13	67,287,668 (GRCm39)	missense	probably benign	0.00
R1880:Zfp759	UTSW	13	67,287,276 (GRCm39)	missense	probably damaging	1.00
R2118:Zfp759	UTSW	13	67,287,578 (GRCm39)	unclassified	probably benign
R2170:Zfp759	UTSW	13	67,284,812 (GRCm39)	missense	possibly damaging	0.88
R3154:Zfp759	UTSW	13	67,286,719 (GRCm39)	missense	probably benign	0.20
R3551:Zfp759	UTSW	13	67,287,031 (GRCm39)	missense	probably benign	0.24
R4392:Zfp759	UTSW	13	67,287,707 (GRCm39)	nonsense	probably null
R4495:Zfp759	UTSW	13	67,286,989 (GRCm39)	splice site	probably null
R4736:Zfp759	UTSW	13	67,287,408 (GRCm39)	missense	probably damaging	1.00
R4882:Zfp759	UTSW	13	67,287,354 (GRCm39)	missense	probably damaging	1.00
R5717:Zfp759	UTSW	13	67,286,772 (GRCm39)	missense	probably damaging	1.00
R5921:Zfp759	UTSW	13	67,288,558 (GRCm39)	missense	probably damaging	1.00
R6247:Zfp759	UTSW	13	67,288,524 (GRCm39)	missense	probably benign	0.00
R6381:Zfp759	UTSW	13	67,286,969 (GRCm39)	nonsense	probably null
R6427:Zfp759	UTSW	13	67,287,162 (GRCm39)	splice site	probably null
R6567:Zfp759	UTSW	13	67,287,150 (GRCm39)	missense	probably benign	0.34
R7140:Zfp759	UTSW	13	67,288,177 (GRCm39)	missense	possibly damaging	0.92
R7731:Zfp759	UTSW	13	67,287,690 (GRCm39)	missense	possibly damaging	0.82
R8504:Zfp759	UTSW	13	67,286,947 (GRCm39)	missense	probably benign	0.00
R8770:Zfp759	UTSW	13	67,288,417 (GRCm39)	missense	probably damaging	1.00
R9695:Zfp759	UTSW	13	67,287,198 (GRCm39)	missense	possibly damaging	0.94
Z1176:Zfp759	UTSW	13	67,284,872 (GRCm39)	missense	probably damaging	0.98
Z1177:Zfp759	UTSW	13	67,288,212 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGACCTTCCGCTTTCCATCA -3'
(R):5'- TACTGGAAAGTCTGCCACATACTTC -3'

Sequencing Primer
(F):5'- CCCTACAAGTGTGAAATATGTGGC -3'
(R):5'- GCCACATACTTCACACTTTTAAGG -3'

Posted On

2022-03-25