Mutagenetix > Incidental Mutation

Incidental Mutation 'R9384:Tas2r134'

710188

Institutional Source

Beutler Lab

Gene Symbol

Tas2r134

Ensembl Gene

ENSMUSG00000056115

Gene Name

taste receptor, type 2, member 134

Synonyms

Tas2r34, T2R134

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.056) question?

Stock #

R9384 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

51517523-51518419 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 51518034 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 171 (Y171F)

Ref Sequence

ENSEMBL: ENSMUSP00000064657 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000070028]

AlphaFold

Q7TQB0

Predicted Effect

probably damaging
Transcript: ENSMUST00000070028
AA Change: Y171F

PolyPhen 2 Score 0.974 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000064657
Gene: ENSMUSG00000056115
AA Change: Y171F

Domain	Start	End	E-Value	Type
Pfam:TAS2R	1	291	1.4e-75	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 47 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A3galt2	G	A	4: 128,655,851 (GRCm39)	C73Y	probably damaging	Het
Agrn	C	T	4: 156,257,106 (GRCm39)	A1213T	probably damaging	Het
Arhgef10	T	C	8: 15,041,067 (GRCm39)	L1079P	probably damaging	Het
Cdcp3	T	C	7: 130,904,404 (GRCm39)	V2374A	unknown	Het
Celsr1	G	T	15: 85,917,286 (GRCm39)	S229*	probably null	Het
Coro6	A	G	11: 77,360,218 (GRCm39)	E436G	probably benign	Het
D430041D05Rik	A	G	2: 104,087,920 (GRCm39)	V352A	probably benign	Het
Dcbld2	C	T	16: 58,285,926 (GRCm39)	T695I	probably damaging	Het
Dclk1	A	C	3: 55,154,936 (GRCm39)	Q122P	possibly damaging	Het
Dsel	C	A	1: 111,787,863 (GRCm39)	E891*	probably null	Het
Eral1	A	T	11: 77,969,130 (GRCm39)	N123K	probably damaging	Het
Fzd6	C	A	15: 38,895,102 (GRCm39)	L423M	probably damaging	Het
Fzd6	T	G	15: 38,895,103 (GRCm39)	L423R	probably damaging	Het
Galntl6	G	T	8: 58,415,461 (GRCm39)	L231I	probably damaging	Het
Gm7137	A	G	10: 77,623,614 (GRCm39)	S141P	unknown	Het
Grm5	T	C	7: 87,723,518 (GRCm39)	Y603H	probably damaging	Het
Hcar2	A	T	5: 124,002,597 (GRCm39)	F302Y	probably benign	Het
Hs3st1	T	G	5: 39,771,962 (GRCm39)	D227A	possibly damaging	Het
Igkv7-33	G	T	6: 70,036,174 (GRCm39)	Q4K	possibly damaging	Het
Itprid1	A	T	6: 55,952,613 (GRCm39)	Q852L	probably damaging	Het
Kcp	C	T	6: 29,496,618 (GRCm39)		probably null	Het
Lrrc23	T	C	6: 124,755,189 (GRCm39)	K116R	possibly damaging	Het
Lrriq1	T	A	10: 103,006,458 (GRCm39)	R1222S	probably benign	Het
Nav3	T	A	10: 109,554,158 (GRCm39)	I1735F	probably damaging	Het
Neto1	A	T	18: 86,413,965 (GRCm39)		probably benign	Het
Nlrp9a	T	A	7: 26,258,158 (GRCm39)	L592Q	probably damaging	Het
Or1e30	C	A	11: 73,677,796 (GRCm39)	Q11K	probably benign	Het
Or5t7	T	A	2: 86,507,357 (GRCm39)	T107S	probably benign	Het
Pcdhb20	T	A	18: 37,638,024 (GRCm39)	H183Q	probably benign	Het
Pde4b	A	G	4: 102,112,448 (GRCm39)	T11A	probably benign	Het
Pou2af3	T	C	9: 51,183,638 (GRCm39)	N112S	probably benign	Het
Ptcd3	A	G	6: 71,874,110 (GRCm39)	S234P	possibly damaging	Het
Rtn4	T	C	11: 29,658,471 (GRCm39)	V875A	probably benign	Het
Slc12a8	T	A	16: 33,466,947 (GRCm39)	C485S	probably benign	Het
Smc1b	A	G	15: 84,950,455 (GRCm39)	S1190P	probably damaging	Het
Sorbs2	A	G	8: 46,258,864 (GRCm39)	R1134G	probably benign	Het
Sugt1	T	C	14: 79,866,388 (GRCm39)	Y336H	probably damaging	Het
Tdrd6	A	T	17: 43,937,783 (GRCm39)	D1088E	probably benign	Het
Tgfbrap1	A	T	1: 43,095,912 (GRCm39)	W509R	probably damaging	Het
Tmem115	T	C	9: 107,411,742 (GRCm39)	V22A	possibly damaging	Het
Tmem260	A	G	14: 48,724,276 (GRCm39)	T223A	probably benign	Het
Tmprss6	G	A	15: 78,328,302 (GRCm39)	H565Y	probably damaging	Het
Ubr4	A	G	4: 139,194,631 (GRCm39)	S1175G	unknown	Het
Uspl1	T	C	5: 149,151,349 (GRCm39)	S850P	probably benign	Het
Vmn2r11	C	A	5: 109,201,266 (GRCm39)	A413S	probably damaging	Het
Vmn2r19	T	G	6: 123,292,923 (GRCm39)	S322A	probably benign	Het
Zfp606	T	A	7: 12,227,935 (GRCm39)	C685*	probably null	Het

Other mutations in Tas2r134

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00562:Tas2r134	APN	2	51,518,100 (GRCm39)	missense	possibly damaging	0.90
IGL00563:Tas2r134	APN	2	51,518,100 (GRCm39)	missense	possibly damaging	0.90
IGL01121:Tas2r134	APN	2	51,518,001 (GRCm39)	missense	probably damaging	1.00
IGL01122:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01128:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01132:Tas2r134	APN	2	51,517,671 (GRCm39)	missense	probably damaging	1.00
IGL01312:Tas2r134	APN	2	51,518,247 (GRCm39)	missense	probably damaging	1.00
IGL01419:Tas2r134	APN	2	51,517,759 (GRCm39)	nonsense	probably null
IGL01774:Tas2r134	APN	2	51,518,160 (GRCm39)	missense	probably benign	0.07
IGL02735:Tas2r134	APN	2	51,517,839 (GRCm39)	missense	probably damaging	1.00
R0799:Tas2r134	UTSW	2	51,518,385 (GRCm39)	missense	probably benign	0.00
R1205:Tas2r134	UTSW	2	51,517,998 (GRCm39)	missense	probably benign	0.00
R2267:Tas2r134	UTSW	2	51,518,249 (GRCm39)	missense	probably benign	0.01
R3736:Tas2r134	UTSW	2	51,517,786 (GRCm39)	missense	probably damaging	1.00
R5254:Tas2r134	UTSW	2	51,517,559 (GRCm39)	missense	probably benign	0.39
R5752:Tas2r134	UTSW	2	51,517,880 (GRCm39)	missense	probably damaging	1.00
R6162:Tas2r134	UTSW	2	51,517,571 (GRCm39)	missense	probably damaging	1.00
R6940:Tas2r134	UTSW	2	51,518,148 (GRCm39)	missense	probably benign	0.00
R6954:Tas2r134	UTSW	2	51,517,782 (GRCm39)	missense	probably benign	0.00
R6996:Tas2r134	UTSW	2	51,517,601 (GRCm39)	missense	probably benign	0.00
R7206:Tas2r134	UTSW	2	51,518,120 (GRCm39)	missense	probably benign	0.02
R7303:Tas2r134	UTSW	2	51,518,145 (GRCm39)	missense	probably benign	0.01
R7348:Tas2r134	UTSW	2	51,518,414 (GRCm39)	missense	possibly damaging	0.92
R7479:Tas2r134	UTSW	2	51,517,541 (GRCm39)	missense	not run
R7575:Tas2r134	UTSW	2	51,518,166 (GRCm39)	missense	probably damaging	1.00
R7686:Tas2r134	UTSW	2	51,518,255 (GRCm39)	missense	possibly damaging	0.54
R8721:Tas2r134	UTSW	2	51,517,571 (GRCm39)	missense	probably damaging	1.00
R9622:Tas2r134	UTSW	2	51,518,358 (GRCm39)	missense	possibly damaging	0.89

Predicted Primers

PCR Primer
(F):5'- TGGGACTTCACCAACACTGTC -3'
(R):5'- GGACTTCAGAGCCATAATGTGAG -3'

Sequencing Primer
(F):5'- GGGACTTCACCAACACTGTCAATTTC -3'
(R):5'- TTCAGAGCCATAATGTGAGCCTGG -3'

Posted On

2022-04-18