Mutagenetix > Incidental Mutation

Incidental Mutation 'R9516:Cep112'

718600

Institutional Source

Beutler Lab

Gene Symbol

Cep112

Ensembl Gene

ENSMUSG00000020728

Gene Name

centrosomal protein 112

Synonyms

Macoco, Ccdc46, 8430407H02Rik, 1700029K01Rik, 1700001M19Rik

MMRRC Submission

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.590) question?

Stock #

R9516 (G1)

Quality Score

209.009

Status

Not validated

Chromosome

Chromosomal Location

108316041-108751441 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 108648514 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 783 (T783A)

Ref Sequence

ENSEMBL: ENSMUSP00000138235 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000061287] [ENSMUST00000106715] [ENSMUST00000106718] [ENSMUST00000106724] [ENSMUST00000130515] [ENSMUST00000132978] [ENSMUST00000146912] [ENSMUST00000150863] [ENSMUST00000182729] [ENSMUST00000133670]

AlphaFold

Q5PR68

Predicted Effect

possibly damaging
Transcript: ENSMUST00000061287
AA Change: T825A

PolyPhen 2 Score 0.907 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000050597
Gene: ENSMUSG00000020728
AA Change: T825A

Domain	Start	End	E-Value	Type
Pfam:DUF4485	13	98	4.8e-31	PFAM
coiled coil region	275	341	N/A	INTRINSIC
coiled coil region	397	477	N/A	INTRINSIC
coiled coil region	504	954	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106715
AA Change: T44A

PolyPhen 2 Score 0.003 (Sensitivity: 0.98; Specificity: 0.44)

SMART Domains

Protein: ENSMUSP00000102326
Gene: ENSMUSG00000020728
AA Change: T44A

Domain	Start	End	E-Value	Type
coiled coil region	11	158	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106718
AA Change: T23A

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000102329
Gene: ENSMUSG00000020728
AA Change: T23A

Domain	Start	End	E-Value	Type
coiled coil region	26	173	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106724

SMART Domains

Protein: ENSMUSP00000102335
Gene: ENSMUSG00000020728

Domain	Start	End	E-Value	Type
coiled coil region	64	102	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129814

Predicted Effect

possibly damaging
Transcript: ENSMUST00000130515
AA Change: T825A

PolyPhen 2 Score 0.907 (Sensitivity: 0.81; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000114569
Gene: ENSMUSG00000020728
AA Change: T825A

Domain	Start	End	E-Value	Type
Pfam:DUF4485	12	99	5.8e-31	PFAM
coiled coil region	275	341	N/A	INTRINSIC
coiled coil region	397	477	N/A	INTRINSIC
coiled coil region	504	954	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000132978
AA Change: T82A

PolyPhen 2 Score 0.036 (Sensitivity: 0.94; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000138124
Gene: ENSMUSG00000020728
AA Change: T82A

Domain	Start	End	E-Value	Type
coiled coil region	54	155	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000146912
AA Change: T210A

PolyPhen 2 Score 0.469 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000120601
Gene: ENSMUSG00000020728
AA Change: T210A

Domain	Start	End	E-Value	Type
coiled coil region	25	111	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000150863
AA Change: T877A

PolyPhen 2 Score 0.021 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000122509
Gene: ENSMUSG00000020728
AA Change: T877A

Domain	Start	End	E-Value	Type
Pfam:DUF4485	12	99	6.8e-28	PFAM
coiled coil region	275	341	N/A	INTRINSIC
coiled coil region	397	477	N/A	INTRINSIC
coiled coil region	504	566	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151755

Predicted Effect

probably damaging
Transcript: ENSMUST00000182729
AA Change: T783A

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000138235
Gene: ENSMUSG00000020728
AA Change: T783A

Domain	Start	End	E-Value	Type
Pfam:DUF4485	12	99	5.4e-31	PFAM
coiled coil region	233	299	N/A	INTRINSIC
coiled coil region	355	435	N/A	INTRINSIC
coiled coil region	462	912	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000182735

Predicted Effect

probably benign
Transcript: ENSMUST00000133670

SMART Domains

Protein: ENSMUSP00000114627
Gene: ENSMUSG00000020728

Domain	Start	End	E-Value	Type
low complexity region	10	29	N/A	INTRINSIC
internal_repeat_2	66	104	4.9e-5	PROSPERO
internal_repeat_1	81	110	2.63e-5	PROSPERO
coiled coil region	123	203	N/A	INTRINSIC
internal_repeat_3	252	264	4.9e-5	PROSPERO
low complexity region	317	328	N/A	INTRINSIC
internal_repeat_2	332	370	4.9e-5	PROSPERO
internal_repeat_3	532	544	4.9e-5	PROSPERO
internal_repeat_1	540	569	2.63e-5	PROSPERO
coiled coil region	571	609	N/A	INTRINSIC

Meta Mutation Damage Score

0.0607

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a coiled-coil domain containing protein that belongs to the cell division control protein 42 effector protein family. In neurons, it localizes to the cytoplasm of dendrites and is also enriched in the nucleus where it interacts with the RNA polymerase III transcriptional repressor Maf1 to regulate gamma-aminobutyric acid A receptor surface expression. In addition, the protein has been identified as a component of the human centrosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2014]

Allele List at MGI

Other mutations in this stock

Total: 105 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3300002I08Rik	T	C	2: 150,153,122 (GRCm39)	N88D	possibly damaging	Het
4933430I17Rik	A	G	4: 62,460,916 (GRCm39)	I264V	probably benign	Het
Abcc8	A	G	7: 45,787,429 (GRCm39)	F591L	probably benign	Het
Abr	G	T	11: 76,310,658 (GRCm39)	T810K	probably damaging	Het
Abracl	T	C	10: 17,894,631 (GRCm39)	E6G	unknown	Het
Adamts3	A	T	5: 89,834,750 (GRCm39)	Y871N	probably damaging	Het
Akap13	T	C	7: 75,354,275 (GRCm39)	Y80H	probably benign	Het
Alas1	A	G	9: 106,115,840 (GRCm39)		probably null	Het
Anapc1	A	C	2: 128,517,633 (GRCm39)	L337R	possibly damaging	Het
Apaf1	T	C	10: 90,915,816 (GRCm39)	Y24C	probably damaging	Het
Arhgef16	A	G	4: 154,365,432 (GRCm39)	V561A	possibly damaging	Het
Atp10b	T	A	11: 43,121,224 (GRCm39)	H962Q	probably benign	Het
Brsk2	A	G	7: 141,546,852 (GRCm39)	T432A	probably benign	Het
C1rl	T	A	6: 124,485,802 (GRCm39)	V391E	probably damaging	Het
Caps2	A	T	10: 112,036,637 (GRCm39)	H399L	probably benign	Het
Ccdc43	C	A	11: 102,577,207 (GRCm39)	K199N	probably benign	Het
Ccdc7a	G	T	8: 129,555,774 (GRCm39)	P1198T	unknown	Het
Cct7	A	G	6: 85,444,625 (GRCm39)	Y423C	possibly damaging	Het
Cep250	A	G	2: 155,833,459 (GRCm39)	T1795A	probably benign	Het
Clasp1	T	G	1: 118,431,560 (GRCm39)	S397A	possibly damaging	Het
Clock	A	C	5: 76,377,227 (GRCm39)	F691V	possibly damaging	Het
Cmklr1	A	G	5: 113,752,341 (GRCm39)	V220A	probably benign	Het
Coro2b	A	T	9: 62,335,291 (GRCm39)	Y298*	probably null	Het
Cspg4b	A	T	13: 113,455,649 (GRCm39)	N565I		Het
Cyp2t4	G	A	7: 26,854,717 (GRCm39)	V66M	possibly damaging	Het
Dlk2	G	A	17: 46,613,432 (GRCm39)	G186D	probably damaging	Het
Dok1	T	C	6: 83,009,972 (GRCm39)	K46E	probably damaging	Het
Dok3	A	G	13: 55,672,186 (GRCm39)	I164T	probably benign	Het
Ercc5	T	A	1: 44,207,041 (GRCm39)	D651E	probably damaging	Het
Erp27	T	C	6: 136,885,066 (GRCm39)	K244R	probably benign	Het
Fhip1a	G	A	3: 85,580,559 (GRCm39)	Q549*	probably null	Het
Flvcr2	T	C	12: 85,793,954 (GRCm39)	V110A	possibly damaging	Het
Gsta3	T	C	1: 21,320,060 (GRCm39)	L22P	probably damaging	Het
Hdac5	T	C	11: 102,093,522 (GRCm39)	T493A	probably benign	Het
Hivep2	T	A	10: 14,005,523 (GRCm39)	I707N	probably benign	Het
Hmox1	A	G	8: 75,823,544 (GRCm39)	N71D	probably benign	Het
Hnrnpr	G	T	4: 136,063,615 (GRCm39)	V342F	probably damaging	Het
Ifi44	T	C	3: 151,438,108 (GRCm39)	D393G	probably damaging	Het
Ighv1-82	A	T	12: 115,916,566 (GRCm39)	F9I	probably damaging	Het
Kalrn	A	G	16: 33,854,864 (GRCm39)	S1999P	probably damaging	Het
Kcnq2	A	G	2: 180,776,753 (GRCm39)	S45P	probably benign	Het
Krt77	A	T	15: 101,769,779 (GRCm39)	Y364N	probably damaging	Het
Kti12	T	C	4: 108,705,476 (GRCm39)	V130A	probably benign	Het
Lama2	T	C	10: 27,100,015 (GRCm39)	E830G	probably benign	Het
Lrrn1	T	A	6: 107,545,505 (GRCm39)	H434Q	probably benign	Het
Med13	A	T	11: 86,179,801 (GRCm39)	N1382K	probably benign	Het
Mrpl42	A	G	10: 95,332,684 (GRCm39)		probably null	Het
Mtor	A	G	4: 148,569,103 (GRCm39)	D1140G	probably benign	Het
Mtrr	A	G	13: 68,720,755 (GRCm39)	I280T	probably benign	Het
Myh4	A	T	11: 67,139,290 (GRCm39)	I536F	probably damaging	Het
Myh4	T	A	11: 67,141,129 (GRCm39)	N730K	probably damaging	Het
Nat10	A	C	2: 103,563,364 (GRCm39)	L545R	probably damaging	Het
Nav3	T	A	10: 109,520,015 (GRCm39)	D2356V	probably damaging	Het
Nbea	A	T	3: 55,937,366 (GRCm39)	S748R	probably damaging	Het
Nlrp1a	T	C	11: 70,998,488 (GRCm39)	T904A	probably benign	Het
Nod2	A	T	8: 89,397,050 (GRCm39)	N820I	probably damaging	Het
Nptx1	A	G	11: 119,433,381 (GRCm39)	V406A	probably damaging	Het
Nrdc	T	A	4: 108,901,863 (GRCm39)	I644K	probably benign	Het
Or4e1	T	C	14: 52,700,873 (GRCm39)	I198V	probably benign	Het
Ostf1	T	A	19: 18,573,735 (GRCm39)	I38F	probably benign	Het
Pacc1	G	A	1: 191,082,004 (GRCm39)	R337Q	probably damaging	Het
Pde4b	G	T	4: 102,462,183 (GRCm39)	D605Y	probably damaging	Het
Pde4d	A	G	13: 109,397,196 (GRCm39)	T3A		Het
Pknox1	A	G	17: 31,822,183 (GRCm39)	I317V	probably damaging	Het
Plekhh2	A	G	17: 84,918,240 (GRCm39)	N1283S	probably benign	Het
Ppp3cb	G	A	14: 20,573,868 (GRCm39)	A289V	probably damaging	Het
Prag1	A	G	8: 36,607,208 (GRCm39)	D983G	probably damaging	Het
Psmd13	C	T	7: 140,478,455 (GRCm39)	T62M		Het
Rab42	C	T	4: 132,029,890 (GRCm39)	V111I		Het
Rabl2	T	A	15: 89,474,631 (GRCm39)		probably null	Het
Rapgef6	T	A	11: 54,582,169 (GRCm39)	S1365R	probably damaging	Het
Rassf4	G	T	6: 116,617,265 (GRCm39)	H247N	possibly damaging	Het
Rbfox1	A	G	16: 7,227,573 (GRCm39)	T363A	probably benign	Het
Rbmyf9	T	A	Y: 3,774,888 (GRCm39)	F28L	probably damaging	Het
Sbf1	T	C	15: 89,184,742 (GRCm39)	D1091G	probably damaging	Het
Scaper	A	G	9: 55,593,275 (GRCm39)	V454A	probably benign	Het
Selenbp2	G	A	3: 94,607,352 (GRCm39)	D258N	probably benign	Het
Sirpa	A	T	2: 129,457,555 (GRCm39)	I210F	probably damaging	Het
Skint4	T	C	4: 112,015,236 (GRCm39)	S434P	probably benign	Het
Slc26a5	G	A	5: 22,016,337 (GRCm39)	Q682*	probably null	Het
Slco4a1	C	T	2: 180,115,943 (GRCm39)	S693F	possibly damaging	Het
Smpd3	A	G	8: 106,992,119 (GRCm39)	F145L	probably benign	Het
Son	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	CATGGACTCCCAGATGTTAGCAACTAGCTCTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCAGTATGGACTCCCAGATGTTAGCAACCAGCTCCATGGACTCCCAGATGTTAGCAAC	16: 91,453,579 (GRCm39)		probably benign	Het
Spata31d1c	A	T	13: 65,184,040 (GRCm39)	L527F	probably damaging	Het
Srd5a3	A	G	5: 76,297,794 (GRCm39)	N199D	probably benign	Het
Stard9	C	G	2: 120,534,564 (GRCm39)	P3607R	probably damaging	Het
Sytl1	A	G	4: 132,986,291 (GRCm39)		probably null	Het
Tas2r126	A	G	6: 42,412,307 (GRCm39)	H280R	probably null	Het
Tcf4	C	T	18: 69,652,944 (GRCm39)		probably benign	Het
Tlcd2	A	G	11: 75,359,112 (GRCm39)	T28A	probably damaging	Het
Tm7sf3	C	T	6: 146,525,179 (GRCm39)	D89N	possibly damaging	Het
Tmem119	A	G	5: 113,933,267 (GRCm39)	L178P	probably damaging	Het
Tmprss7	T	C	16: 45,484,564 (GRCm39)	D532G	probably benign	Het
Tnfaip8l3	A	G	9: 53,934,777 (GRCm39)	I66T	probably benign	Het
Tor1b	A	G	2: 30,843,185 (GRCm39)	Y50C	probably damaging	Het
Tpp2	T	A	1: 44,017,648 (GRCm39)	S751T	probably benign	Het
Tpra1	T	C	6: 88,887,221 (GRCm39)	V193A	probably benign	Het
Trip12	A	T	1: 84,735,215 (GRCm39)	V932E	probably damaging	Het
Ttn	T	A	2: 76,777,104 (GRCm39)	T1479S	unknown	Het
Unc13c	A	T	9: 73,392,220 (GRCm39)	V2044D	probably damaging	Het
Usp25	T	G	16: 76,852,076 (GRCm39)	V197G	probably damaging	Het
Vmn2r34	A	G	7: 7,675,366 (GRCm39)	V674A	probably benign	Het
Zfp7	T	C	15: 76,775,484 (GRCm39)	S509P	probably damaging	Het
Zfp821	A	G	8: 110,447,856 (GRCm39)	T66A	probably damaging	Het
Zfp97	T	A	17: 17,365,930 (GRCm39)	N476K	possibly damaging	Het

Other mutations in Cep112

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00679:Cep112	APN	11	108,481,879 (GRCm39)	missense	probably damaging	1.00
IGL00705:Cep112	APN	11	108,362,859 (GRCm39)	missense	probably benign
IGL00848:Cep112	APN	11	108,362,886 (GRCm39)	missense	probably damaging	1.00
IGL00975:Cep112	APN	11	108,325,012 (GRCm39)	missense	probably damaging	1.00
IGL01085:Cep112	APN	11	108,377,432 (GRCm39)	missense	probably damaging	1.00
IGL01286:Cep112	APN	11	108,750,235 (GRCm39)	critical splice donor site	probably null
IGL01536:Cep112	APN	11	108,422,237 (GRCm39)	missense	probably null	0.08
IGL02622:Cep112	APN	11	108,409,509 (GRCm39)	missense	probably benign	0.26
IGL02720:Cep112	APN	11	108,750,177 (GRCm39)	missense	probably damaging	0.98
FR4976:Cep112	UTSW	11	108,316,178 (GRCm39)	unclassified	probably benign
PIT4466001:Cep112	UTSW	11	108,410,722 (GRCm39)	missense	probably benign
R0727:Cep112	UTSW	11	108,397,380 (GRCm39)	missense	probably damaging	1.00
R0907:Cep112	UTSW	11	108,461,258 (GRCm39)	splice site	probably benign
R0908:Cep112	UTSW	11	108,555,323 (GRCm39)	missense	possibly damaging	0.69
R1236:Cep112	UTSW	11	108,750,200 (GRCm39)	missense	probably damaging	1.00
R1514:Cep112	UTSW	11	108,362,880 (GRCm39)	missense	probably damaging	1.00
R2049:Cep112	UTSW	11	108,497,151 (GRCm39)	missense	probably damaging	0.96
R2058:Cep112	UTSW	11	108,410,087 (GRCm39)	critical splice donor site	probably null
R2059:Cep112	UTSW	11	108,410,087 (GRCm39)	critical splice donor site	probably null
R2126:Cep112	UTSW	11	108,399,084 (GRCm39)	missense	probably damaging	0.98
R2142:Cep112	UTSW	11	108,497,151 (GRCm39)	missense	probably damaging	0.96
R2196:Cep112	UTSW	11	108,461,187 (GRCm39)	missense	probably damaging	0.98
R2276:Cep112	UTSW	11	108,746,671 (GRCm39)	missense	probably damaging	1.00
R2414:Cep112	UTSW	11	108,643,408 (GRCm39)	missense	possibly damaging	0.91
R2655:Cep112	UTSW	11	108,328,027 (GRCm39)	splice site	probably benign
R2882:Cep112	UTSW	11	108,410,038 (GRCm39)	missense	possibly damaging	0.94
R3001:Cep112	UTSW	11	108,331,329 (GRCm39)	missense	probably damaging	1.00
R3002:Cep112	UTSW	11	108,331,329 (GRCm39)	missense	probably damaging	1.00
R3003:Cep112	UTSW	11	108,331,329 (GRCm39)	missense	probably damaging	1.00
R4407:Cep112	UTSW	11	108,410,027 (GRCm39)	missense	possibly damaging	0.93
R4796:Cep112	UTSW	11	108,377,818 (GRCm39)	critical splice donor site	probably null
R4898:Cep112	UTSW	11	108,397,471 (GRCm39)	missense	probably damaging	0.96
R4899:Cep112	UTSW	11	108,497,110 (GRCm39)	missense	probably damaging	0.96
R4977:Cep112	UTSW	11	108,325,062 (GRCm39)	missense	probably damaging	0.97
R5021:Cep112	UTSW	11	108,361,154 (GRCm39)	missense	possibly damaging	0.86
R5186:Cep112	UTSW	11	108,643,386 (GRCm39)	missense	probably benign	0.00
R5462:Cep112	UTSW	11	108,409,570 (GRCm39)	missense	probably damaging	1.00
R5494:Cep112	UTSW	11	108,555,431 (GRCm39)	missense	probably damaging	1.00
R5506:Cep112	UTSW	11	108,555,429 (GRCm39)	missense	probably damaging	1.00
R5560:Cep112	UTSW	11	108,328,061 (GRCm39)	missense	probably damaging	1.00
R5682:Cep112	UTSW	11	108,361,138 (GRCm39)	missense	probably damaging	1.00
R5857:Cep112	UTSW	11	108,422,297 (GRCm39)	splice site	probably benign
R5863:Cep112	UTSW	11	108,497,058 (GRCm39)	missense	probably damaging	1.00
R5884:Cep112	UTSW	11	108,461,142 (GRCm39)	missense	probably damaging	0.99
R5913:Cep112	UTSW	11	108,648,514 (GRCm39)	missense	probably damaging	0.99
R6344:Cep112	UTSW	11	108,410,000 (GRCm39)	missense	probably damaging	0.98
R6498:Cep112	UTSW	11	108,331,357 (GRCm39)	missense	probably benign	0.25
R6611:Cep112	UTSW	11	108,397,377 (GRCm39)	missense	possibly damaging	0.71
R6638:Cep112	UTSW	11	108,746,696 (GRCm39)	missense	probably damaging	1.00
R6916:Cep112	UTSW	11	108,750,202 (GRCm39)	missense	probably damaging	1.00
R7182:Cep112	UTSW	11	108,573,670 (GRCm39)	missense	probably benign	0.07
R7262:Cep112	UTSW	11	108,555,467 (GRCm39)	missense	probably damaging	0.99
R7386:Cep112	UTSW	11	108,699,507 (GRCm39)	missense	probably benign	0.00
R7539:Cep112	UTSW	11	108,746,654 (GRCm39)	missense	probably benign	0.38
R8262:Cep112	UTSW	11	108,393,977 (GRCm39)	missense	probably damaging	1.00
R8681:Cep112	UTSW	11	108,316,478 (GRCm39)	critical splice donor site	probably null
R8845:Cep112	UTSW	11	108,461,193 (GRCm39)	missense	probably damaging	1.00
R8955:Cep112	UTSW	11	108,643,260 (GRCm39)	missense	possibly damaging	0.61
R9213:Cep112	UTSW	11	108,377,779 (GRCm39)	missense	probably benign
R9348:Cep112	UTSW	11	108,328,076 (GRCm39)	missense	probably damaging	0.97
R9771:Cep112	UTSW	11	108,573,517 (GRCm39)	intron	probably benign
R9784:Cep112	UTSW	11	108,461,217 (GRCm39)	missense	probably damaging	1.00
Z1176:Cep112	UTSW	11	108,316,136 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- AGCAGTTGTTCACCATTCACAG -3'
(R):5'- CATGAAAGCAGCTGGTACCAC -3'

Sequencing Primer
(F):5'- ACCATTCACAGTTTTATTGGAGCC -3'
(R):5'- AGCAGCTGGTACCACTGGTTG -3'

Posted On

2022-07-18