Mutagenetix > Incidental Mutation

Incidental Mutation 'R9732:Etl4'

731442

Institutional Source

Beutler Lab

Gene Symbol

Etl4

Ensembl Gene

ENSMUSG00000036617

Gene Name

enhancer trap locus 4

Synonyms

6620402G01Rik, Sickle tail, E330027G05Rik, Etl-4, Skt, 9430077C05Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably essential (E-score: 0.809) question?

Stock #

R9732 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

19915018-20815346 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 20748373 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 237 (T237I)

Ref Sequence

ENSEMBL: ENSMUSP00000110257 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000045555] [ENSMUST00000066509] [ENSMUST00000114604] [ENSMUST00000114606] [ENSMUST00000114607] [ENSMUST00000114608] [ENSMUST00000114610] [ENSMUST00000114614] [ENSMUST00000114627]

AlphaFold

A2AQ25

Predicted Effect

probably damaging
Transcript: ENSMUST00000045555
AA Change: T317I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000041431
Gene: ENSMUSG00000036617
AA Change: T317I

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1067	1096	N/A	INTRINSIC
low complexity region	1212	1231	N/A	INTRINSIC
low complexity region	1296	1314	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000066509
AA Change: T317I

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000066170
Gene: ENSMUSG00000036617
AA Change: T317I

Domain	Start	End	E-Value	Type
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1372	1381	N/A	INTRINSIC
low complexity region	1470	1495	N/A	INTRINSIC
low complexity region	1571	1582	N/A	INTRINSIC
coiled coil region	1658	1686	N/A	INTRINSIC
low complexity region	1724	1737	N/A	INTRINSIC
low complexity region	1806	1825	N/A	INTRINSIC
low complexity region	1890	1908	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114604
AA Change: T317I

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000110251
Gene: ENSMUSG00000036617
AA Change: T317I

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	655	687	N/A	INTRINSIC
low complexity region	1102	1131	N/A	INTRINSIC
low complexity region	1207	1226	N/A	INTRINSIC
low complexity region	1291	1309	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114606
AA Change: T35I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110253
Gene: ENSMUSG00000036617
AA Change: T35I

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114607
AA Change: T35I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110254
Gene: ENSMUSG00000036617
AA Change: T35I

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114608
AA Change: T35I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110255
Gene: ENSMUSG00000036617
AA Change: T35I

Domain	Start	End	E-Value	Type
low complexity region	31	46	N/A	INTRINSIC
low complexity region	68	86	N/A	INTRINSIC
coiled coil region	338	370	N/A	INTRINSIC
low complexity region	785	814	N/A	INTRINSIC
low complexity region	1055	1064	N/A	INTRINSIC
low complexity region	1153	1178	N/A	INTRINSIC
low complexity region	1254	1265	N/A	INTRINSIC
coiled coil region	1341	1369	N/A	INTRINSIC
low complexity region	1407	1420	N/A	INTRINSIC
low complexity region	1489	1508	N/A	INTRINSIC
low complexity region	1573	1591	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114610
AA Change: T237I

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000110257
Gene: ENSMUSG00000036617
AA Change: T237I

Domain	Start	End	E-Value	Type
Pfam:AIP3	108	211	5e-12	PFAM
low complexity region	233	248	N/A	INTRINSIC
low complexity region	270	288	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114614
AA Change: T317I

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000110261
Gene: ENSMUSG00000036617
AA Change: T317I

Domain	Start	End	E-Value	Type
Pfam:AIP3	188	291	1.7e-11	PFAM
low complexity region	313	328	N/A	INTRINSIC
low complexity region	350	368	N/A	INTRINSIC
coiled coil region	620	652	N/A	INTRINSIC
low complexity region	1056	1085	N/A	INTRINSIC
low complexity region	1201	1220	N/A	INTRINSIC
low complexity region	1285	1303	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000114627
AA Change: T368I

PolyPhen 2 Score 0.998 (Sensitivity: 0.27; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000110274
Gene: ENSMUSG00000036617
AA Change: T368I

Domain	Start	End	E-Value	Type
low complexity region	21	38	N/A	INTRINSIC
low complexity region	60	72	N/A	INTRINSIC
Pfam:AIP3	239	341	2.4e-14	PFAM
low complexity region	364	379	N/A	INTRINSIC
low complexity region	401	419	N/A	INTRINSIC
Pfam:AIP3	600	841	1.1e-12	PFAM
low complexity region	1153	1182	N/A	INTRINSIC
low complexity region	1423	1432	N/A	INTRINSIC
low complexity region	1521	1546	N/A	INTRINSIC
low complexity region	1622	1633	N/A	INTRINSIC
coiled coil region	1709	1737	N/A	INTRINSIC
low complexity region	1775	1788	N/A	INTRINSIC
low complexity region	1857	1876	N/A	INTRINSIC
low complexity region	1941	1959	N/A	INTRINSIC

Coding Region Coverage

1x: 99.9%
3x: 99.8%
10x: 99.5%
20x: 99.0%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Mice homozygous for a gene-trapped allele display malformations of the notochord and caudal vertebrae and may exhibit caudal tail kinks. Mice homozygous for another gene-trapped allele have malformed caudal vertebrae and intervertebral disk abnormalities; about half display kinked tails. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 76 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agrn	A	T	4: 156,258,446 (GRCm39)	S1007T	probably benign	Het
Akap10	A	T	11: 61,787,545 (GRCm39)	C466S	probably damaging	Het
Apol9b	C	T	15: 77,619,566 (GRCm39)	P121S	possibly damaging	Het
Atr	T	A	9: 95,743,438 (GRCm39)	Y26N	probably damaging	Het
Cadm2	G	A	16: 66,528,297 (GRCm39)	T346I	probably benign	Het
Capn7	T	C	14: 31,090,031 (GRCm39)	I628T	probably damaging	Het
Cfap43	A	G	19: 47,775,446 (GRCm39)	S609P	probably damaging	Het
Chst2	C	T	9: 95,287,951 (GRCm39)	G132S	probably benign	Het
Clip2	C	T	5: 134,533,616 (GRCm39)	R487Q	probably benign	Het
Coq10a	A	G	10: 128,199,516 (GRCm39)	F253L	probably benign	Het
Cyp2t4	C	T	7: 26,854,657 (GRCm39)	P46S	probably damaging	Het
Ddx54	T	C	5: 120,763,911 (GRCm39)		probably null	Het
Dip2a	T	A	10: 76,110,077 (GRCm39)	I1180L	probably benign	Het
Dpp8	G	T	9: 64,970,862 (GRCm39)		probably null	Het
Efr3a	T	A	15: 65,720,139 (GRCm39)	N378K	probably benign	Het
Exo1	T	A	1: 175,727,065 (GRCm39)	S459T	probably benign	Het
Fam81b	G	A	13: 76,399,985 (GRCm39)	T91I	probably benign	Het
Fry	A	G	5: 150,328,758 (GRCm39)	E1297G	probably benign	Het
Gm11110	G	A	17: 57,410,382 (GRCm39)	L39F	unknown	Het
Gm3233	G	T	10: 77,595,147 (GRCm39)	Q124K	unknown	Het
Gm7145	C	G	1: 117,913,839 (GRCm39)	H240Q	probably benign	Het
Gnrh1	G	T	14: 67,985,316 (GRCm39)	R67L	possibly damaging	Het
Grip2	T	A	6: 91,761,686 (GRCm39)	E236V	probably damaging	Het
H3c10	A	T	13: 21,902,186 (GRCm39)	I120F	probably benign	Het
Hectd4	G	T	5: 121,392,254 (GRCm39)	E173*	probably null	Het
Hgf	T	A	5: 16,820,748 (GRCm39)	L632I	probably damaging	Het
Igsf11	T	C	16: 38,843,652 (GRCm39)	V255A	probably benign	Het
Jmjd4	G	T	11: 59,341,339 (GRCm39)		probably null	Het
Khdrbs3	C	A	15: 68,885,212 (GRCm39)	N71K	probably damaging	Het
Kif20b	A	G	19: 34,930,353 (GRCm39)	D1286G	probably benign	Het
Lpin3	T	A	2: 160,734,196 (GRCm39)	L27Q	probably damaging	Het
Macroh2a1	G	C	13: 56,243,976 (GRCm39)	F183L	probably benign	Het
Mcm5	C	T	8: 75,844,168 (GRCm39)	S313F	probably benign	Het
Mipep	T	A	14: 61,033,637 (GRCm39)	F184I	probably damaging	Het
Mst1	A	T	9: 107,959,425 (GRCm39)	D237V	possibly damaging	Het
Mycbp2	T	C	14: 103,448,749 (GRCm39)	D1803G	probably damaging	Het
Ncoa6	T	G	2: 155,244,636 (GRCm39)	K1978T	probably damaging	Het
Nkain4	G	A	2: 180,585,901 (GRCm39)	T54I	probably damaging	Het
Olig3	G	A	10: 19,233,151 (GRCm39)	A259T	probably benign	Het
Or5w14	T	C	2: 87,541,489 (GRCm39)	T254A	possibly damaging	Het
Pacs1	A	G	19: 5,184,997 (GRCm39)	W943R	probably damaging	Het
Pkp4	T	A	2: 59,138,797 (GRCm39)	L349Q	possibly damaging	Het
Polh	A	T	17: 46,498,997 (GRCm39)	H239Q	probably benign	Het
Ptprq	G	C	10: 107,412,767 (GRCm39)	C1777W	probably damaging	Het
Rhod	A	T	19: 4,476,740 (GRCm39)	V127E	probably damaging	Het
Rnf6	A	G	5: 146,152,931 (GRCm39)	S84P	probably benign	Het
Rundc3b	T	C	5: 8,562,406 (GRCm39)	T321A	probably damaging	Het
Rxfp2	G	A	5: 149,993,767 (GRCm39)	A620T	probably damaging	Het
Sacm1l	A	G	9: 123,381,863 (GRCm39)	E155G	probably benign	Het
Saxo5	C	T	8: 3,526,167 (GRCm39)	H107Y	possibly damaging	Het
Sdad1	A	T	5: 92,438,942 (GRCm39)	D471E	probably benign	Het
Sema6a	T	A	18: 47,381,925 (GRCm39)	N874I	probably damaging	Het
Serinc4	C	A	2: 121,283,631 (GRCm39)	W283L	possibly damaging	Het
Simc1	GCAGTCACTAAGAAGTGTAATGCAGTCATCAGGAGGTGTGACACAGTCACTAAGAAGTGTGATGCAGTCACCAGGAGGTGTGA	GCAGTCACTAAGAAGTGTGATGCAGTCACCAGGAGGTGTGA	13: 54,673,177 (GRCm39)		probably benign	Het
Slc24a3	A	T	2: 145,458,591 (GRCm39)	S524C	probably damaging	Het
Slc4a10	T	G	2: 62,135,086 (GRCm39)	S1042R	probably damaging	Het
Smg7	A	T	1: 152,736,212 (GRCm39)	V190D	probably damaging	Het
Sub1	T	A	15: 11,986,650 (GRCm39)	I66F	possibly damaging	Het
Svop	G	A	5: 114,201,142 (GRCm39)	P109S	probably damaging	Het
Synj1	G	A	16: 90,761,414 (GRCm39)	P684L	probably damaging	Het
Synm	A	G	7: 67,385,652 (GRCm39)	V670A	probably damaging	Het
Tep1	T	C	14: 51,088,162 (GRCm39)	I792V	probably benign	Het
Tex22	A	G	12: 113,052,196 (GRCm39)	T85A	possibly damaging	Het
Tmem151a	C	T	19: 5,131,937 (GRCm39)	S423N	probably damaging	Het
Tnks1bp1	T	A	2: 84,889,727 (GRCm39)	W685R	probably damaging	Het
Top3a	A	G	11: 60,640,391 (GRCm39)	F436L	probably benign	Het
Trim55	A	T	3: 19,716,039 (GRCm39)	I200F	probably damaging	Het
Trpv6	T	A	6: 41,603,862 (GRCm39)	R186*	probably null	Het
Ttc6	G	A	12: 57,775,335 (GRCm39)	C1677Y	probably benign	Het
Ttn	C	T	2: 76,769,206 (GRCm39)	V2831I	unknown	Het
Tyrp1	T	A	4: 80,758,930 (GRCm39)	S268T	possibly damaging	Het
Unc5d	C	T	8: 29,381,319 (GRCm39)		probably null	Het
Usb1	G	T	8: 96,065,375 (GRCm39)	V129L	probably damaging	Het
Usp22	G	A	11: 61,051,437 (GRCm39)	T302M	probably damaging	Het
Vmn1r210	C	A	13: 23,011,379 (GRCm39)	L302F	possibly damaging	Het
Zfp689	C	A	7: 127,044,283 (GRCm39)	E116*	probably null	Het

Other mutations in Etl4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00907:Etl4	APN	2	20,771,289 (GRCm39)	missense	possibly damaging	0.81
IGL00944:Etl4	APN	2	20,534,865 (GRCm39)	missense	possibly damaging	0.52
IGL01078:Etl4	APN	2	20,811,342 (GRCm39)	nonsense	probably null
IGL01099:Etl4	APN	2	20,811,922 (GRCm39)	missense	probably benign	0.06
IGL01337:Etl4	APN	2	20,790,198 (GRCm39)	missense	probably benign	0.01
IGL01348:Etl4	APN	2	20,811,784 (GRCm39)	missense	probably damaging	1.00
IGL01349:Etl4	APN	2	20,718,207 (GRCm39)	missense	probably damaging	1.00
IGL01407:Etl4	APN	2	20,748,667 (GRCm39)	missense	probably damaging	0.99
IGL01552:Etl4	APN	2	20,783,000 (GRCm39)	missense	probably damaging	0.99
IGL01662:Etl4	APN	2	20,811,460 (GRCm39)	missense	probably benign	0.04
IGL01687:Etl4	APN	2	20,534,898 (GRCm39)	missense	probably damaging	1.00
IGL01793:Etl4	APN	2	20,748,709 (GRCm39)	missense	possibly damaging	0.87
IGL01844:Etl4	APN	2	20,811,493 (GRCm39)	missense	probably benign	0.06
IGL02025:Etl4	APN	2	20,811,337 (GRCm39)	missense	probably damaging	1.00
IGL02088:Etl4	APN	2	20,811,359 (GRCm39)	missense	probably damaging	1.00
IGL02134:Etl4	APN	2	20,811,240 (GRCm39)	missense	possibly damaging	0.79
IGL02369:Etl4	APN	2	20,535,000 (GRCm39)	missense	probably damaging	1.00
IGL02480:Etl4	APN	2	20,793,335 (GRCm39)	missense	probably damaging	0.99
IGL02560:Etl4	APN	2	20,748,529 (GRCm39)	missense	probably damaging	1.00
IGL02851:Etl4	APN	2	20,812,840 (GRCm39)	missense	possibly damaging	0.46
IGL02893:Etl4	APN	2	20,765,021 (GRCm39)	splice site	probably benign
IGL02951:Etl4	APN	2	20,806,348 (GRCm39)	splice site	probably benign
IGL03119:Etl4	APN	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
IGL03267:Etl4	APN	2	20,789,993 (GRCm39)	nonsense	probably null
IGL03379:Etl4	APN	2	20,666,827 (GRCm39)	missense	possibly damaging	0.87
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0038:Etl4	UTSW	2	20,748,385 (GRCm39)	missense	probably damaging	1.00
R0095:Etl4	UTSW	2	20,748,679 (GRCm39)	missense	probably damaging	1.00
R0100:Etl4	UTSW	2	20,344,716 (GRCm39)	missense	probably benign
R0311:Etl4	UTSW	2	20,811,940 (GRCm39)	missense	probably damaging	1.00
R0346:Etl4	UTSW	2	20,764,463 (GRCm39)	critical splice donor site	probably null
R0348:Etl4	UTSW	2	20,782,940 (GRCm39)	missense	probably damaging	1.00
R0379:Etl4	UTSW	2	20,812,165 (GRCm39)	missense	probably damaging	0.98
R0571:Etl4	UTSW	2	20,748,580 (GRCm39)	missense	probably damaging	0.99
R0697:Etl4	UTSW	2	20,748,672 (GRCm39)	missense	probably damaging	1.00
R0707:Etl4	UTSW	2	20,810,382 (GRCm39)	splice site	probably benign
R0980:Etl4	UTSW	2	20,806,378 (GRCm39)	missense	probably damaging	1.00
R1120:Etl4	UTSW	2	20,811,514 (GRCm39)	missense	probably benign	0.00
R1254:Etl4	UTSW	2	20,812,734 (GRCm39)	missense	probably damaging	1.00
R1346:Etl4	UTSW	2	20,810,955 (GRCm39)	missense	possibly damaging	0.94
R1460:Etl4	UTSW	2	20,793,288 (GRCm39)	missense	probably damaging	1.00
R1503:Etl4	UTSW	2	20,748,685 (GRCm39)	missense	possibly damaging	0.94
R1547:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R1627:Etl4	UTSW	2	20,806,390 (GRCm39)	missense	possibly damaging	0.91
R1635:Etl4	UTSW	2	20,811,219 (GRCm39)	missense	probably damaging	1.00
R1716:Etl4	UTSW	2	20,748,492 (GRCm39)	missense	probably damaging	1.00
R1795:Etl4	UTSW	2	20,812,837 (GRCm39)	critical splice donor site	probably null
R1885:Etl4	UTSW	2	20,748,795 (GRCm39)	missense	probably damaging	1.00
R2039:Etl4	UTSW	2	20,790,039 (GRCm39)	missense	probably damaging	1.00
R2083:Etl4	UTSW	2	20,748,360 (GRCm39)	missense	probably damaging	1.00
R2109:Etl4	UTSW	2	20,790,153 (GRCm39)	missense	probably benign	0.27
R2153:Etl4	UTSW	2	20,803,545 (GRCm39)	missense	probably benign	0.00
R2403:Etl4	UTSW	2	20,812,117 (GRCm39)	nonsense	probably null
R2883:Etl4	UTSW	2	20,810,985 (GRCm39)	missense	possibly damaging	0.83
R2985:Etl4	UTSW	2	20,786,660 (GRCm39)	missense	probably damaging	1.00
R3402:Etl4	UTSW	2	20,786,693 (GRCm39)	missense	probably damaging	1.00
R3696:Etl4	UTSW	2	20,806,473 (GRCm39)	critical splice donor site	probably null
R3755:Etl4	UTSW	2	20,748,348 (GRCm39)	missense	probably benign	0.10
R3813:Etl4	UTSW	2	20,793,246 (GRCm39)	missense	probably damaging	1.00
R3829:Etl4	UTSW	2	20,790,232 (GRCm39)	missense	probably benign	0.07
R3887:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3888:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3889:Etl4	UTSW	2	20,534,772 (GRCm39)	nonsense	probably null
R3958:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3959:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R3960:Etl4	UTSW	2	20,344,854 (GRCm39)	missense	probably benign
R4058:Etl4	UTSW	2	20,810,830 (GRCm39)	missense	possibly damaging	0.59
R4074:Etl4	UTSW	2	20,814,030 (GRCm39)	utr 3 prime	probably benign
R4077:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4078:Etl4	UTSW	2	20,812,772 (GRCm39)	missense	probably damaging	1.00
R4127:Etl4	UTSW	2	20,748,886 (GRCm39)	missense	possibly damaging	0.93
R4200:Etl4	UTSW	2	20,786,694 (GRCm39)	missense	probably damaging	1.00
R4492:Etl4	UTSW	2	20,811,676 (GRCm39)	missense	possibly damaging	0.67
R4514:Etl4	UTSW	2	20,666,709 (GRCm39)	missense	probably damaging	1.00
R4820:Etl4	UTSW	2	20,811,496 (GRCm39)	missense	possibly damaging	0.85
R4825:Etl4	UTSW	2	20,811,738 (GRCm39)	missense	probably damaging	1.00
R4888:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R4938:Etl4	UTSW	2	20,803,460 (GRCm39)	missense	probably benign	0.00
R4943:Etl4	UTSW	2	20,812,092 (GRCm39)	missense	probably benign	0.05
R5121:Etl4	UTSW	2	20,344,922 (GRCm39)	critical splice donor site	probably null
R5191:Etl4	UTSW	2	20,344,810 (GRCm39)	missense	probably damaging	0.99
R5198:Etl4	UTSW	2	20,718,198 (GRCm39)	missense	probably damaging	1.00
R5199:Etl4	UTSW	2	20,748,853 (GRCm39)	missense	probably damaging	1.00
R5470:Etl4	UTSW	2	20,534,791 (GRCm39)	missense	probably damaging	0.99
R5513:Etl4	UTSW	2	20,748,638 (GRCm39)	missense	probably damaging	1.00
R5620:Etl4	UTSW	2	20,535,037 (GRCm39)	missense	probably damaging	1.00
R5635:Etl4	UTSW	2	20,811,846 (GRCm39)	missense	probably damaging	1.00
R5641:Etl4	UTSW	2	20,811,273 (GRCm39)	frame shift	probably null
R5690:Etl4	UTSW	2	20,810,647 (GRCm39)	missense	probably benign	0.01
R5784:Etl4	UTSW	2	20,811,016 (GRCm39)	missense	possibly damaging	0.79
R5794:Etl4	UTSW	2	20,811,323 (GRCm39)	missense	probably damaging	1.00
R5908:Etl4	UTSW	2	20,748,718 (GRCm39)	missense	probably damaging	0.96
R5982:Etl4	UTSW	2	20,785,826 (GRCm39)	missense	probably damaging	1.00
R6151:Etl4	UTSW	2	20,718,171 (GRCm39)	missense	probably damaging	1.00
R6192:Etl4	UTSW	2	20,806,362 (GRCm39)	missense	probably damaging	0.98
R6238:Etl4	UTSW	2	20,806,379 (GRCm39)	missense	probably damaging	1.00
R6248:Etl4	UTSW	2	20,813,900 (GRCm39)	missense	possibly damaging	0.90
R6292:Etl4	UTSW	2	20,748,384 (GRCm39)	missense	probably damaging	1.00
R6610:Etl4	UTSW	2	20,718,180 (GRCm39)	missense	probably damaging	1.00
R6739:Etl4	UTSW	2	20,718,246 (GRCm39)	missense	probably damaging	1.00
R6846:Etl4	UTSW	2	20,748,919 (GRCm39)	missense	possibly damaging	0.94
R6863:Etl4	UTSW	2	20,811,120 (GRCm39)	missense	probably benign	0.01
R6873:Etl4	UTSW	2	20,802,803 (GRCm39)	splice site	probably null
R7003:Etl4	UTSW	2	20,810,695 (GRCm39)	missense	probably benign	0.03
R7155:Etl4	UTSW	2	20,811,742 (GRCm39)	missense	probably damaging	0.96
R7207:Etl4	UTSW	2	20,714,387 (GRCm39)	missense	probably damaging	0.99
R7230:Etl4	UTSW	2	20,802,799 (GRCm39)	missense	probably damaging	1.00
R7305:Etl4	UTSW	2	20,714,368 (GRCm39)	missense	probably damaging	1.00
R7389:Etl4	UTSW	2	20,789,904 (GRCm39)	nonsense	probably null
R7396:Etl4	UTSW	2	20,803,449 (GRCm39)	missense	possibly damaging	0.62
R7441:Etl4	UTSW	2	20,749,000 (GRCm39)	missense	possibly damaging	0.87
R7626:Etl4	UTSW	2	20,718,189 (GRCm39)	missense	probably damaging	1.00
R7776:Etl4	UTSW	2	20,811,957 (GRCm39)	missense	probably damaging	0.99
R7779:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R7798:Etl4	UTSW	2	20,786,757 (GRCm39)	critical splice donor site	probably null
R7851:Etl4	UTSW	2	20,748,951 (GRCm39)	missense	probably damaging	1.00
R7861:Etl4	UTSW	2	20,810,721 (GRCm39)	missense	probably benign
R7901:Etl4	UTSW	2	20,294,821 (GRCm39)	missense	possibly damaging	0.83
R8053:Etl4	UTSW	2	20,666,774 (GRCm39)	missense	probably damaging	1.00
R8124:Etl4	UTSW	2	20,811,451 (GRCm39)	missense	probably benign	0.06
R8133:Etl4	UTSW	2	20,811,082 (GRCm39)	missense	possibly damaging	0.86
R8203:Etl4	UTSW	2	20,789,916 (GRCm39)	missense	possibly damaging	0.61
R8238:Etl4	UTSW	2	20,811,342 (GRCm39)	nonsense	probably null
R8263:Etl4	UTSW	2	20,748,965 (GRCm39)	missense	probably benign	0.00
R8299:Etl4	UTSW	2	20,748,874 (GRCm39)	missense	possibly damaging	0.81
R8318:Etl4	UTSW	2	20,793,341 (GRCm39)	missense	probably damaging	1.00
R8334:Etl4	UTSW	2	20,785,857 (GRCm39)	missense	probably damaging	0.96
R8443:Etl4	UTSW	2	20,810,977 (GRCm39)	missense	probably benign	0.04
R8525:Etl4	UTSW	2	20,534,892 (GRCm39)	missense	probably damaging	1.00
R8679:Etl4	UTSW	2	20,714,288 (GRCm39)	missense	probably damaging	1.00
R8918:Etl4	UTSW	2	20,811,246 (GRCm39)	missense	probably benign	0.00
R8918:Etl4	UTSW	2	20,748,733 (GRCm39)	missense	probably benign
R9062:Etl4	UTSW	2	20,748,616 (GRCm39)	missense	probably damaging	0.99
R9095:Etl4	UTSW	2	20,782,964 (GRCm39)	missense	probably damaging	1.00
R9200:Etl4	UTSW	2	20,786,702 (GRCm39)	missense	probably damaging	1.00
R9416:Etl4	UTSW	2	20,748,784 (GRCm39)	missense	probably benign	0.17
R9437:Etl4	UTSW	2	20,813,872 (GRCm39)	missense	probably benign	0.20
R9451:Etl4	UTSW	2	20,813,926 (GRCm39)	missense	probably benign	0.03
R9489:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9531:Etl4	UTSW	2	20,294,818 (GRCm39)	start codon destroyed	probably null	0.01
R9605:Etl4	UTSW	2	20,771,345 (GRCm39)	missense	possibly damaging	0.75
R9623:Etl4	UTSW	2	20,811,052 (GRCm39)	missense
R9631:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9632:Etl4	UTSW	2	20,666,749 (GRCm39)	missense	probably benign	0.28
R9646:Etl4	UTSW	2	20,802,724 (GRCm39)	missense	probably benign	0.00
R9755:Etl4	UTSW	2	20,790,048 (GRCm39)	missense	probably benign	0.17
R9771:Etl4	UTSW	2	20,811,537 (GRCm39)	missense	probably benign
RF003:Etl4	UTSW	2	20,524,729 (GRCm39)	nonsense	probably null
X0018:Etl4	UTSW	2	20,814,001 (GRCm39)	missense	probably damaging	0.98
X0022:Etl4	UTSW	2	20,714,375 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCACCTTGTCACATGAGACCC -3'
(R):5'- CGGCATAGAAACCCTCATTTCTG -3'

Sequencing Primer
(F):5'- TGTCACATGAGACCCCTAAAAACATG -3'
(R):5'- GAACATAACCAGGTTTTTGCTGCTC -3'

Posted On

2022-11-14