Mutagenetix > Incidental Mutation

Incidental Mutation 'R1582:Mslnl'

171501

Institutional Source

Beutler Lab

Gene Symbol

Mslnl

Ensembl Gene

ENSMUSG00000041062

Gene Name

mesothelin-like

Synonyms

MMRRC Submission

039619-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R1582 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

25955016-25967304 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 25961908 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Methionine at position 128 (V128M)

Ref Sequence

ENSEMBL: ENSMUSP00000049020 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000047098]

AlphaFold

Q8C160

Predicted Effect

probably damaging
Transcript: ENSMUST00000047098
AA Change: V128M

PolyPhen 2 Score 0.971 (Sensitivity: 0.77; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062
AA Change: V128M

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
Pfam:Mesothelin	29	589	2.8e-70	PFAM
low complexity region	633	653	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000102319

Meta Mutation Damage Score

0.2148

Coding Region Coverage

1x: 99.0%
3x: 98.0%
10x: 95.4%
20x: 89.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abl1	C	G	2: 31,690,371 (GRCm39)	A630G	probably damaging	Het
Actr3	A	G	1: 125,333,662 (GRCm39)	Y202H	probably benign	Het
Adamts19	A	G	18: 59,103,013 (GRCm39)	N685D	probably damaging	Het
Atl3	A	G	19: 7,494,264 (GRCm39)	T138A	probably damaging	Het
Bpifa2	T	C	2: 153,855,638 (GRCm39)	S188P	probably damaging	Het
Bsn	T	C	9: 107,982,291 (GRCm39)	T3821A	unknown	Het
Dcun1d2	A	G	8: 13,330,926 (GRCm39)	L68P	probably damaging	Het
Ddhd1	A	T	14: 45,842,566 (GRCm39)	L630I	probably damaging	Het
Ddx25	T	C	9: 35,457,272 (GRCm39)	T348A	probably damaging	Het
Dtnb	C	T	12: 3,823,554 (GRCm39)	T580M	possibly damaging	Het
Dysf	A	G	6: 84,074,749 (GRCm39)	S561G	probably damaging	Het
Ehbp1l1	A	G	19: 5,771,995 (GRCm39)	I101T	possibly damaging	Het
Erich3	T	C	3: 154,469,960 (GRCm39)		probably benign	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fam135a	T	C	1: 24,068,398 (GRCm39)	T611A	probably damaging	Het
Gpbp1	T	C	13: 111,573,066 (GRCm39)		probably null	Het
H2-Oa	A	T	17: 34,313,695 (GRCm39)	T218S	probably damaging	Het
Kctd19	G	A	8: 106,122,092 (GRCm39)	L152F	probably damaging	Het
Kdm5b	T	A	1: 134,552,591 (GRCm39)	M1189K	probably damaging	Het
Kif13a	A	G	13: 46,947,398 (GRCm39)	V862A	probably benign	Het
Lct	T	C	1: 128,228,299 (GRCm39)	T1065A	probably damaging	Het
Mark3	G	A	12: 111,621,744 (GRCm39)	A697T	probably benign	Het
Mosmo	A	G	7: 120,329,728 (GRCm39)	I116M	possibly damaging	Het
Mpped1	C	T	15: 83,676,191 (GRCm39)		probably benign	Het
Muc15	A	T	2: 110,567,817 (GRCm39)	M321L	probably benign	Het
Naa25	T	A	5: 121,572,892 (GRCm39)	N670K	probably benign	Het
Or1e1c	T	A	11: 73,266,090 (GRCm39)	C172S	probably damaging	Het
Or5e1	G	T	7: 108,354,317 (GRCm39)	V85L	probably benign	Het
Or6z3	A	G	7: 6,463,813 (GRCm39)	M102V	probably benign	Het
Ppara	T	C	15: 85,682,429 (GRCm39)	I375T	possibly damaging	Het
Prss27	A	G	17: 24,263,877 (GRCm39)	I188V	probably benign	Het
Rbms1	A	T	2: 60,589,179 (GRCm39)	M287K	possibly damaging	Het
Relt	C	T	7: 100,500,560 (GRCm39)		probably null	Het
Rsf1	CG	CGACGGCGGGG	7: 97,229,115 (GRCm39)		probably benign	Het
Sec24a	A	G	11: 51,599,794 (GRCm39)	V837A	probably benign	Het
Smarca2	G	A	19: 26,729,305 (GRCm39)	D19N	probably damaging	Het
Sorbs2	G	A	8: 46,258,814 (GRCm39)	G620D	probably damaging	Het
Spata31d1c	C	A	13: 65,181,038 (GRCm39)	Q46K	probably benign	Het
Spata6	C	G	4: 111,637,992 (GRCm39)	S274*	probably null	Het
Spata6	C	T	4: 111,637,994 (GRCm39)	P275S	probably benign	Het
Tas2r108	T	A	6: 40,470,566 (GRCm39)	V14D	probably benign	Het
Thbs2	G	T	17: 14,891,550 (GRCm39)	P996T	probably damaging	Het
Tmem131l	T	C	3: 83,839,090 (GRCm39)	Q620R	probably damaging	Het
Ubap2l	A	G	3: 89,941,978 (GRCm39)	S203P	probably damaging	Het
Unc45b	G	A	11: 82,816,771 (GRCm39)	G404S	probably benign	Het
Uri1	A	G	7: 37,664,811 (GRCm39)	S292P	possibly damaging	Het
Usp21	A	T	1: 171,110,655 (GRCm39)	C444S	probably damaging	Het
Vmn1r117	A	T	7: 20,617,484 (GRCm39)	V188D	possibly damaging	Het
Vmn1r7	T	C	6: 57,002,143 (GRCm39)	D39G	probably damaging	Het
Wdr25	T	C	12: 108,863,980 (GRCm39)	F42L	possibly damaging	Het

Other mutations in Mslnl

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01621:Mslnl	APN	17	25,962,641 (GRCm39)	unclassified	probably benign
IGL01629:Mslnl	APN	17	25,963,749 (GRCm39)	missense	possibly damaging	0.95
IGL02084:Mslnl	APN	17	25,965,125 (GRCm39)	missense	probably benign	0.07
IGL02408:Mslnl	APN	17	25,966,972 (GRCm39)	missense	possibly damaging	0.80
IGL02726:Mslnl	APN	17	25,963,077 (GRCm39)	critical splice donor site	probably null
IGL03387:Mslnl	APN	17	25,963,051 (GRCm39)	missense	probably benign	0.06
R0561:Mslnl	UTSW	17	25,962,177 (GRCm39)	nonsense	probably null
R0881:Mslnl	UTSW	17	25,961,939 (GRCm39)	missense	possibly damaging	0.82
R1295:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1296:Mslnl	UTSW	17	25,962,214 (GRCm39)	missense	probably damaging	1.00
R1629:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1630:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1631:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1632:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1794:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1850:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1866:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1876:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R1914:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2166:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2241:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2243:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2247:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2282:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2284:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2852:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2867:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2877:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2878:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2919:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R2920:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3026:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3405:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3406:Mslnl	UTSW	17	25,965,155 (GRCm39)	missense	probably damaging	1.00
R3411:Mslnl	UTSW	17	25,963,491 (GRCm39)	missense	probably benign	0.05
R3434:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3546:Mslnl	UTSW	17	25,963,943 (GRCm39)	missense	probably damaging	0.98
R3612:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3729:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3730:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3802:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3804:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3894:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R3895:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4454:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4455:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4456:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4457:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4561:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4562:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4564:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4600:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4601:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4610:Mslnl	UTSW	17	25,961,908 (GRCm39)	missense	probably damaging	0.97
R4704:Mslnl	UTSW	17	25,957,952 (GRCm39)	missense	possibly damaging	0.73
R5155:Mslnl	UTSW	17	25,957,942 (GRCm39)	nonsense	probably null
R5257:Mslnl	UTSW	17	25,965,139 (GRCm39)	missense	probably benign	0.00
R5456:Mslnl	UTSW	17	25,962,133 (GRCm39)	missense	probably damaging	0.98
R5645:Mslnl	UTSW	17	25,956,816 (GRCm39)	missense	possibly damaging	0.95
R6007:Mslnl	UTSW	17	25,965,749 (GRCm39)	missense	probably benign	0.00
R6083:Mslnl	UTSW	17	25,956,876 (GRCm39)	missense	possibly damaging	0.83
R6142:Mslnl	UTSW	17	25,963,531 (GRCm39)	missense	probably damaging	1.00
R6761:Mslnl	UTSW	17	25,965,047 (GRCm39)	missense	probably damaging	1.00
R7058:Mslnl	UTSW	17	25,962,186 (GRCm39)	missense	probably benign	0.03
R7156:Mslnl	UTSW	17	25,962,184 (GRCm39)	missense	probably benign	0.20
R7467:Mslnl	UTSW	17	25,955,895 (GRCm39)	start codon destroyed	probably benign	0.33
R7687:Mslnl	UTSW	17	25,962,157 (GRCm39)	missense	probably damaging	0.97
R7807:Mslnl	UTSW	17	25,965,751 (GRCm39)	missense	probably benign	0.03
R8682:Mslnl	UTSW	17	25,965,962 (GRCm39)	missense	probably benign
R8735:Mslnl	UTSW	17	25,964,062 (GRCm39)	missense	probably benign	0.09
R8742:Mslnl	UTSW	17	25,964,047 (GRCm39)	missense	probably damaging	1.00
R9208:Mslnl	UTSW	17	25,961,694 (GRCm39)	missense	possibly damaging	0.94
R9264:Mslnl	UTSW	17	25,961,506 (GRCm39)	intron	probably benign
RF007:Mslnl	UTSW	17	25,962,202 (GRCm39)	missense	possibly damaging	0.87

Predicted Primers

PCR Primer
(F):5'- CTTTTCTACGAGTGAGTGCCCCTG -3'
(R):5'- GCAGTCACAATTTGTGCTGCCTCC -3'

Sequencing Primer
(F):5'- TGCCCCTGCCATCAGAAG -3'
(R):5'- TCTGAAGCACTGAGCTGTAGG -3'

Posted On

2014-04-13