Mutagenetix > Incidental Mutations

Incidental Mutation 'R3684:Zfp810'

269471

Institutional Source

Beutler Lab

Gene Symbol

Zfp810

Ensembl Gene

ENSMUSG00000066829

Gene Name

zinc finger protein 810

Synonyms

MMRRC Submission

040682-MU

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.079) question?

Stock #

R3684 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

22276748-22307648 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 22278235 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 459 (D459G)

Ref Sequence

ENSEMBL: ENSMUSP00000083459 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086278] [ENSMUST00000215202]

AlphaFold

Q99K45

Predicted Effect

probably benign
Transcript: ENSMUST00000086278
AA Change: D459G

PolyPhen 2 Score 0.007 (Sensitivity: 0.96; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000083459
Gene: ENSMUSG00000066829
AA Change: D459G

Domain	Start	End	E-Value	Type
KRAB	4	64	1.09e-33	SMART
ZnF_C2H2	126	148	2.44e2	SMART
ZnF_C2H2	182	204	3.07e-1	SMART
ZnF_C2H2	210	232	8.47e-4	SMART
ZnF_C2H2	238	260	6.78e-3	SMART
ZnF_C2H2	266	288	6.13e-1	SMART
ZnF_C2H2	294	316	5.06e-2	SMART
ZnF_C2H2	322	344	4.79e-3	SMART
ZnF_C2H2	350	372	2.99e-4	SMART
ZnF_C2H2	378	400	1.33e-1	SMART
ZnF_C2H2	406	428	2.75e-3	SMART
ZnF_C2H2	434	456	1.58e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000214499

Predicted Effect

probably benign
Transcript: ENSMUST00000215202

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700001P01Rik	A	T	11: 97,775,699	Y54N	probably damaging	Het
Adamdec1	C	T	14: 68,581,998	V17I	probably benign	Het
Ahdc1	T	C	4: 133,065,702	L1418P	possibly damaging	Het
Atr	T	C	9: 95,920,400	S1782P	probably damaging	Het
Btbd9	T	A	17: 30,334,307	N394Y	probably damaging	Het
Calb2	A	G	8: 110,156,988	Y35H	probably benign	Het
Cdon	A	G	9: 35,489,032	E1014G	possibly damaging	Het
Cep83	A	G	10: 94,786,825	T588A	probably benign	Het
Cfap126	T	C	1: 171,114,031	S32P	possibly damaging	Het
Clcn7	T	C	17: 25,150,593	L301P	possibly damaging	Het
Corin	T	C	5: 72,330,855	D610G	probably damaging	Het
Dok3	A	G	13: 55,524,493	S154P	probably damaging	Het
Ggta1	T	A	2: 35,407,988	T162S	probably benign	Het
Gldn	G	A	9: 54,338,340	E392K	possibly damaging	Het
Gls	A	T	1: 52,166,293	D447E	probably damaging	Het
Itih5	A	T	2: 10,238,624	N391Y	possibly damaging	Het
Jchain	G	A	5: 88,522,539	P74S	probably damaging	Het
Lct	T	C	1: 128,304,226	M629V	probably damaging	Het
Lrrn1	T	C	6: 107,567,949	V236A	probably benign	Het
Mcm3ap	T	C	10: 76,489,426	S954P	possibly damaging	Het
Myh11	T	C	16: 14,203,234	N1725S	probably benign	Het
Ppcdc	C	T	9: 57,421,125		probably null	Het
Rhobtb3	A	G	13: 75,939,481	I129T	probably damaging	Het
Sfxn2	A	G	19: 46,591,153	R252G	probably benign	Het
Sh2d2a	C	A	3: 87,851,720		probably null	Het
Sh3gl1	T	C	17: 56,018,953	K159E	possibly damaging	Het
Slc7a9	A	G	7: 35,453,501	T115A	probably benign	Het
Synj2	A	T	17: 6,028,443	D1020V	probably damaging	Het
Tenm2	C	A	11: 36,051,817	V1342L	probably benign	Het
Tmem127	T	A	2: 127,248,732	I56N	possibly damaging	Het
Traj7	A	G	14: 54,211,481		probably benign	Het
Unc5d	A	G	8: 28,694,592	F627L	probably damaging	Het
Unc79	T	A	12: 103,074,803	N698K	probably benign	Het
Usp17la	A	T	7: 104,861,730	N514I	possibly damaging	Het
Uvrag	A	T	7: 98,988,220	C341S	probably damaging	Het

Other mutations in Zfp810

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00484:Zfp810	APN	9	22278309	nonsense	probably null
IGL03079:Zfp810	APN	9	22284127	missense	probably damaging	1.00
IGL03402:Zfp810	APN	9	22279145	splice site	probably null
H8562:Zfp810	UTSW	9	22279091	missense	probably benign	0.42
R1116:Zfp810	UTSW	9	22279085	missense	probably benign	0.11
R1160:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R1171:Zfp810	UTSW	9	22278826	missense	possibly damaging	0.95
R1393:Zfp810	UTSW	9	22280514	missense	probably benign
R1608:Zfp810	UTSW	9	22278920	missense	probably benign	0.00
R1644:Zfp810	UTSW	9	22279028	missense	possibly damaging	0.67
R1766:Zfp810	UTSW	9	22278532	missense	possibly damaging	0.64
R2568:Zfp810	UTSW	9	22279238	missense	probably benign	0.01
R4002:Zfp810	UTSW	9	22278892	missense	probably damaging	1.00
R4134:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4135:Zfp810	UTSW	9	22279073	missense	probably damaging	0.97
R4334:Zfp810	UTSW	9	22278784	missense	probably benign	0.00
R4545:Zfp810	UTSW	9	22278745	missense	probably damaging	0.96
R5399:Zfp810	UTSW	9	22278829	missense	possibly damaging	0.91
R5622:Zfp810	UTSW	9	22279096	missense	probably benign	0.00
R5643:Zfp810	UTSW	9	22283171	missense	probably benign	0.26
R7375:Zfp810	UTSW	9	22290537	critical splice donor site	probably null
R7441:Zfp810	UTSW	9	22279272	nonsense	probably null
R7809:Zfp810	UTSW	9	22278982	missense	possibly damaging	0.51
R8422:Zfp810	UTSW	9	22283222	nonsense	probably null
R8526:Zfp810	UTSW	9	22278290	missense	probably damaging	1.00
R8719:Zfp810	UTSW	9	22279275	missense	probably benign	0.00
R9177:Zfp810	UTSW	9	22278640	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACATTCCCTAAAGTAATAGATAGGTCT -3'
(R):5'- ACTGCAAGTCTCACCTCACTGT -3'

Sequencing Primer
(F):5'- CTATACATGGAGATGAGCTAGAGACC -3'
(R):5'- TGCAGGAAAGCTTTCTACCG -3'

Posted On

2015-02-19