Mutagenetix > Incidental Mutation

Incidental Mutation 'R4805:Or2t29'

370634

Institutional Source

Beutler Lab

Gene Symbol

Or2t29

Ensembl Gene

ENSMUSG00000064252

Gene Name

olfactory receptor family 2 subfamily T member 29

Synonyms

GA_x6K02T2NKPP-882068-883006, Olfr329-ps, MOR275-6P, Olfr329

MMRRC Submission

041999-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R4805 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

58433273-58434309 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 58433396 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 315 (T315I)

Ref Sequence

ENSEMBL: ENSMUSP00000104450 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108822] [ENSMUST00000108823] [ENSMUST00000134055] [ENSMUST00000219448]

AlphaFold

M9MMK4

Predicted Effect

probably benign
Transcript: ENSMUST00000108822
AA Change: T315I

PolyPhen 2 Score 0.033 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000104450
Gene: ENSMUSG00000064252
AA Change: T315I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	29	306	1.1e-47	PFAM
Pfam:7TM_GPCR_Srsx	33	299	5.1e-7	PFAM
Pfam:7tm_1	39	288	4.6e-28	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000108823
AA Change: T302I

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000104451
Gene: ENSMUSG00000064252
AA Change: T302I

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	33	299	5.1e-7	PFAM
Pfam:7tm_1	39	288	4.4e-35	PFAM
Pfam:7tm_4	137	281	5.8e-43	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000134055
AA Change: T302I

SMART Domains

Protein: ENSMUSP00000145126
Gene: ENSMUSG00000050818
AA Change: T302I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	35	312	1.6e-46	PFAM
Pfam:7TM_GPCR_Srsx	39	309	4.4e-6	PFAM
Pfam:7tm_1	45	294	2.3e-27	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000204252

SMART Domains

Protein: ENSMUSP00000145167
Gene: ENSMUSG00000064252

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	30	158	4.3e-6	PFAM
Pfam:7tm_4	35	157	5.9e-19	PFAM
Pfam:7tm_1	45	158	2.9e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000219448
AA Change: T302I

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.1%
3x: 98.3%
10x: 96.4%
20x: 92.7%

Validation Efficiency

99% (69/70)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930407I10Rik	T	A	15: 81,950,628 (GRCm39)	Y1508*	probably null	Het
4931414P19Rik	T	C	14: 54,832,911 (GRCm39)	E88G	probably benign	Het
Acaa1b	A	G	9: 118,986,014 (GRCm39)	S17P	probably benign	Het
Arhgef7	G	A	8: 11,881,552 (GRCm39)	E565K	probably damaging	Het
Arl6ip1	AAAATAAATAAATAAATAAATAAATA	AAAATAAATAAATAAATAAATAAATAAATA	7: 117,721,122 (GRCm39)		probably benign	Het
Bpnt1	T	A	1: 185,077,504 (GRCm39)		probably null	Het
Ccnt1	C	T	15: 98,442,189 (GRCm39)	V360I	probably benign	Het
Chd4	T	C	6: 125,105,908 (GRCm39)	S1829P	possibly damaging	Het
Dennd1b	A	T	1: 138,981,122 (GRCm39)	N112I	probably damaging	Het
Fat2	A	T	11: 55,174,805 (GRCm39)	D1969E	probably benign	Het
Flywch1	A	C	17: 23,979,591 (GRCm39)	D394E	probably benign	Het
Gal3st4	T	A	5: 138,263,733 (GRCm39)		probably null	Het
Gm14403	T	C	2: 177,200,492 (GRCm39)	L146P	probably damaging	Het
Gm5283	G	T	3: 17,285,123 (GRCm39)		noncoding transcript	Het
Gpr161	T	A	1: 165,134,029 (GRCm39)	L97Q	probably damaging	Het
Gstm2	A	T	3: 107,892,411 (GRCm39)	M105K	possibly damaging	Het
Hecw2	A	G	1: 53,880,018 (GRCm39)	Y1264H	probably damaging	Het
Ifi206	T	A	1: 173,308,952 (GRCm39)	Q348L	possibly damaging	Het
Ighv1-83	A	T	12: 115,927,489 (GRCm39)	M87K	possibly damaging	Het
Ighv2-7	A	T	12: 113,771,154 (GRCm39)	S44T	probably damaging	Het
Ipo7	T	A	7: 109,650,691 (GRCm39)	N884K	probably benign	Het
Itga2b	T	C	11: 102,358,692 (GRCm39)	N75S	probably benign	Het
Krt20	A	G	11: 99,319,811 (GRCm39)	V431A	unknown	Het
Krt24	A	T	11: 99,174,452 (GRCm39)	I205N	possibly damaging	Het
Lmo2	T	C	2: 103,811,407 (GRCm39)	Y147H	probably damaging	Het
Lrrc31	A	T	3: 30,745,446 (GRCm39)	L67*	probably null	Het
Lrrc37a	A	G	11: 103,395,135 (GRCm39)	F97L	probably benign	Het
Mapkap1	G	A	2: 34,487,434 (GRCm39)		probably null	Het
Mgat4d	T	G	8: 84,084,787 (GRCm39)		probably null	Het
Mrgprb3	C	T	7: 48,293,054 (GRCm39)	V166I	probably benign	Het
Myh11	T	A	16: 14,052,329 (GRCm39)	Q379L	possibly damaging	Het
Myh7	T	C	14: 55,222,590 (GRCm39)	S782G	probably benign	Het
Nobox	T	C	6: 43,282,053 (GRCm39)	D340G	probably damaging	Het
Ntn4	C	T	10: 93,480,362 (GRCm39)	R29C	probably damaging	Het
Or5b101	T	A	19: 13,005,661 (GRCm39)	I11F	probably benign	Het
Pikfyve	T	A	1: 65,307,959 (GRCm39)	I1845N	probably damaging	Het
Pramel15	A	G	4: 144,103,590 (GRCm39)	Y179H	probably benign	Het
Rad21l	T	G	2: 151,509,927 (GRCm39)	H58P	probably damaging	Het
Scnn1g	T	C	7: 121,345,825 (GRCm39)	L316S	probably damaging	Het
Serpina5	G	A	12: 104,068,460 (GRCm39)	V174I	probably damaging	Het
Shank1	T	C	7: 43,993,135 (GRCm39)	S788P	unknown	Het
Shisal1	T	A	15: 84,301,397 (GRCm39)	H82L	probably damaging	Het
Snap47	A	G	11: 59,319,343 (GRCm39)	V265A	possibly damaging	Het
Steap3	T	A	1: 120,171,616 (GRCm39)	H163L	probably benign	Het
Sycp2	T	C	2: 178,035,754 (GRCm39)		probably benign	Het
Tet2	A	G	3: 133,173,076 (GRCm39)	F1729L	probably benign	Het
Thsd7b	T	C	1: 130,116,276 (GRCm39)	V1343A	probably benign	Het
Thumpd2	G	A	17: 81,334,130 (GRCm39)	T486I	probably damaging	Het
Ttc41	G	T	10: 86,565,662 (GRCm39)	M405I	possibly damaging	Het
Ttn	T	C	2: 76,577,873 (GRCm39)	D24340G	probably damaging	Het
Vmn1r10	T	G	6: 57,090,602 (GRCm39)	F65V	possibly damaging	Het
Vmn2r118	G	T	17: 55,899,581 (GRCm39)	D774E	probably damaging	Het
Vmn2r13	C	T	5: 109,304,331 (GRCm39)	C700Y	probably damaging	Het
Wsb1	A	C	11: 79,131,217 (GRCm39)	M393R	possibly damaging	Het
Zmat3	A	G	3: 32,397,504 (GRCm39)	Y214H	probably benign	Het
Zswim3	C	T	2: 164,662,099 (GRCm39)	T193I	possibly damaging	Het

Other mutations in Or2t29

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0744:Or2t29	UTSW	11	58,433,988 (GRCm39)	missense	possibly damaging	0.76
R2426:Or2t29	UTSW	11	58,433,920 (GRCm39)	nonsense	probably null
R6498:Or2t29	UTSW	11	58,433,408 (GRCm39)	missense	probably damaging	1.00
R7341:Or2t29	UTSW	11	58,433,533 (GRCm39)	nonsense	probably null
R7768:Or2t29	UTSW	11	58,433,693 (GRCm39)	missense	possibly damaging	0.94
R7768:Or2t29	UTSW	11	58,433,466 (GRCm39)	missense	probably damaging	0.99
R7957:Or2t29	UTSW	11	58,433,624 (GRCm39)	missense	probably damaging	1.00
R8416:Or2t29	UTSW	11	58,433,778 (GRCm39)	missense	possibly damaging	0.75
R9076:Or2t29	UTSW	11	58,433,782 (GRCm39)	nonsense	probably null
R9606:Or2t29	UTSW	11	58,433,753 (GRCm39)	missense	probably damaging	1.00
Z1186:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign
Z1187:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign
Z1188:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign
Z1189:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign
Z1190:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign
Z1191:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign
Z1192:Or2t29	UTSW	11	58,434,272 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- TGCAAACTGGACAGATTACAAGTAG -3'
(R):5'- CTCAGGATGAACTCAGCAGAGG -3'

Sequencing Primer
(F):5'- CACAATGATTCTTCAAAAAGGGATG -3'
(R):5'- CAGGAAGAAGGCCCTTGC -3'

Posted On

2016-02-04