Mutagenetix > Incidental Mutation

Incidental Mutation 'R4884:Col27a1'

375506

Institutional Source

Beutler Lab

Gene Symbol

Col27a1

Ensembl Gene

ENSMUSG00000045672

Gene Name

collagen, type XXVII, alpha 1

Synonyms

5730512J02Rik

MMRRC Submission

041978-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4884 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

63132246-63253228 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 63194197 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 851 (D851E)

Ref Sequence

ENSEMBL: ENSMUSP00000043816 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000036300] [ENSMUST00000184067]

AlphaFold

Q5QNQ9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000036300
AA Change: D851E

PolyPhen 2 Score 0.768 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000043816
Gene: ENSMUSG00000045672
AA Change: D851E

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
TSPN	43	223	1.1e-5	SMART
low complexity region	325	343	N/A	INTRINSIC
low complexity region	356	372	N/A	INTRINSIC
low complexity region	428	443	N/A	INTRINSIC
low complexity region	455	467	N/A	INTRINSIC
low complexity region	584	597	N/A	INTRINSIC
Pfam:Collagen	609	670	2.1e-10	PFAM
Pfam:Collagen	666	731	3.7e-10	PFAM
low complexity region	790	808	N/A	INTRINSIC
low complexity region	817	838	N/A	INTRINSIC
low complexity region	858	880	N/A	INTRINSIC
low complexity region	886	910	N/A	INTRINSIC
low complexity region	912	946	N/A	INTRINSIC
Pfam:Collagen	1012	1080	2.8e-8	PFAM
Pfam:Collagen	1033	1103	3e-9	PFAM
Pfam:Collagen	1063	1130	3.4e-9	PFAM
low complexity region	1150	1168	N/A	INTRINSIC
Pfam:Collagen	1207	1281	5.5e-9	PFAM
Pfam:Collagen	1261	1324	8.4e-10	PFAM
Pfam:Collagen	1323	1384	3.8e-12	PFAM
low complexity region	1438	1466	N/A	INTRINSIC
internal_repeat_4	1467	1502	1.5e-7	PROSPERO
internal_repeat_2	1468	1529	1.96e-8	PROSPERO
Pfam:Collagen	1544	1606	2.4e-9	PFAM
COLFI	1644	1845	1.28e-40	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000125504

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000148751

Predicted Effect

unknown
Transcript: ENSMUST00000184067
AA Change: D236E

SMART Domains

Protein: ENSMUSP00000139173
Gene: ENSMUSG00000045672
AA Change: D236E

Domain	Start	End	E-Value	Type
Pfam:Collagen	23	87	2.1e-8	PFAM
Pfam:Collagen	57	145	8.3e-8	PFAM
Pfam:Collagen	115	200	9.9e-8	PFAM
low complexity region	202	223	N/A	INTRINSIC
low complexity region	243	265	N/A	INTRINSIC
low complexity region	271	295	N/A	INTRINSIC
low complexity region	297	331	N/A	INTRINSIC
internal_repeat_1	337	442	5.17e-20	PROSPERO
Pfam:Collagen	448	515	1.5e-9	PFAM
Pfam:Collagen	478	543	2e-10	PFAM
Pfam:Collagen	502	566	2.5e-9	PFAM
Pfam:Collagen	532	617	4.4e-7	PFAM
Pfam:Collagen	594	660	8.2e-11	PFAM
Pfam:Collagen	649	709	1.4e-10	PFAM
Pfam:Collagen	708	769	2e-12	PFAM
Pfam:Collagen	752	829	5e-8	PFAM
Pfam:Collagen	878	939	2.2e-9	PFAM

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.7%
20x: 93.5%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes the alpha-1 subunit of type XXVII collagen, one of the low abundance fibril-forming collagens found in cartilage. The encoded protein forms a homotrimeric triple helical procollagen that undergoes proteolytic processing during fibril formation. Transgenic mice lacking a portion of the collagenous domain in the encoded protein exhibit skeletal abnormalities, chondrodysplasia and die at birth because of a lung defect. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for an in frame deletion display neonatal lethality, respiratory failure, and severe chondrodysplasia. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 113 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
Aadat	C	T	8: 60,979,663 (GRCm39)	P175L	probably damaging	Het
Acot7	G	T	4: 152,270,664 (GRCm39)		probably benign	Het
Adad1	T	G	3: 37,130,813 (GRCm39)	F259V	possibly damaging	Het
Adora2a	A	G	10: 75,161,879 (GRCm39)	Y6C	probably null	Het
Ahnak	A	G	19: 8,990,118 (GRCm39)		probably benign	Het
Ankar	A	T	1: 72,737,966 (GRCm39)	M72K	probably damaging	Het
Ankrd45	A	G	1: 160,988,270 (GRCm39)	K176R	possibly damaging	Het
Ap3m2	T	C	8: 23,293,997 (GRCm39)	K18E	probably damaging	Het
Apol10b	C	T	15: 77,473,006 (GRCm39)	R16Q	possibly damaging	Het
Atp2b2	T	A	6: 113,819,147 (GRCm39)	T49S	possibly damaging	Het
B3gntl1	T	G	11: 121,520,795 (GRCm39)	Y206S	possibly damaging	Het
BC061237	G	A	14: 44,738,666 (GRCm39)	E22K	possibly damaging	Het
Bmp1	A	G	14: 70,712,655 (GRCm39)	V959A	probably benign	Het
Cep295	T	C	9: 15,263,056 (GRCm39)	E169G	probably damaging	Het
Cfap20dc	G	A	14: 8,578,394 (GRCm38)	T116I	probably damaging	Het
Cfap65	T	G	1: 74,942,283 (GRCm39)	E1757A	possibly damaging	Het
Cgrrf1	T	A	14: 47,090,912 (GRCm39)	I216N	possibly damaging	Het
Clstn2	T	A	9: 97,681,448 (GRCm39)	D64V	probably damaging	Het
Coq9	C	T	8: 95,579,822 (GRCm39)	P259L	probably benign	Het
Cps1	A	G	1: 67,216,183 (GRCm39)	N836S	probably benign	Het
Cracd	A	T	5: 76,996,682 (GRCm39)	I47F	probably damaging	Het
Crebbp	T	C	16: 3,906,239 (GRCm39)	K1588E	probably damaging	Het
Cspg4	T	C	9: 56,805,353 (GRCm39)	W2055R	probably benign	Het
Cyp3a44	A	T	5: 145,714,792 (GRCm39)	M453K	probably damaging	Het
Dhx36	T	G	3: 62,391,681 (GRCm39)	D555A	probably damaging	Het
Dock2	A	T	11: 34,216,248 (GRCm39)	Y1219N	probably damaging	Het
Dpy19l2	A	T	9: 24,539,476 (GRCm39)	C492*	probably null	Het
Dusp22	A	G	13: 30,852,813 (GRCm39)	N16S	probably benign	Het
Epha1	A	G	6: 42,337,668 (GRCm39)	M805T	probably damaging	Het
Espl1	C	T	15: 102,232,505 (GRCm39)	A2071V	possibly damaging	Het
Fbxo11	T	A	17: 88,299,761 (GRCm39)	D863V	probably damaging	Het
Fbxo7	T	A	10: 85,865,014 (GRCm39)	Y106N	probably damaging	Het
Fhip1a	A	C	3: 85,590,918 (GRCm39)	C178G	probably damaging	Het
Fst	A	G	13: 114,590,920 (GRCm39)	V282A	probably damaging	Het
Gfra3	T	A	18: 34,844,304 (GRCm39)	M79L	probably benign	Het
Glp1r	T	C	17: 31,155,240 (GRCm39)	V409A	probably damaging	Het
Gm10799	T	A	2: 103,898,552 (GRCm39)	D51V	probably damaging	Het
Gm572	C	A	4: 148,751,819 (GRCm39)	T228N	possibly damaging	Het
Grip1	C	T	10: 119,911,211 (GRCm39)	T643M	probably damaging	Het
Hdgfl2	C	T	17: 56,403,265 (GRCm39)	R222C	possibly damaging	Het
Hecw2	T	A	1: 53,990,000 (GRCm39)	I125F	probably benign	Het
Hipk1	A	G	3: 103,651,338 (GRCm39)	S1153P	possibly damaging	Het
Insm2	T	C	12: 55,646,546 (GRCm39)	S97P	probably damaging	Het
Iqca1	A	G	1: 90,067,759 (GRCm39)	V164A	probably benign	Het
Kcna6	T	C	6: 126,715,689 (GRCm39)	D400G	probably benign	Het
Kctd1	T	C	18: 15,107,311 (GRCm39)	Y122C	probably damaging	Het
Klk14	G	A	7: 43,341,501 (GRCm39)	C51Y	probably damaging	Het
Klra2	T	C	6: 131,207,165 (GRCm39)	Y148C	probably damaging	Het
Krtap31-1	G	A	11: 99,799,310 (GRCm39)	C171Y	unknown	Het
Ldlrap1	C	T	4: 134,486,282 (GRCm39)	R59Q	probably benign	Het
Ltb	T	C	17: 35,414,234 (GRCm39)	I3T	probably benign	Het
Macf1	T	C	4: 123,348,802 (GRCm39)	I2477V	probably benign	Het
Mpdz	A	T	4: 81,279,713 (GRCm39)	I39N	probably damaging	Het
Mtcl3	A	T	10: 29,072,537 (GRCm39)	N610Y	probably damaging	Het
Mycbp2	A	G	14: 103,448,731 (GRCm39)	I1776T	probably damaging	Het
Myof	T	A	19: 37,930,805 (GRCm39)	E994V	probably damaging	Het
Myom3	A	G	4: 135,510,366 (GRCm39)	E553G	possibly damaging	Het
Mysm1	A	T	4: 94,847,185 (GRCm39)	C504S	probably damaging	Het
Nectin3	A	T	16: 46,269,249 (GRCm39)	H384Q	probably benign	Het
Net1	G	A	13: 3,934,252 (GRCm39)	R482*	probably null	Het
Nit1	T	C	1: 171,171,263 (GRCm39)	K193R	probably null	Het
Nlgn1	A	G	3: 25,966,838 (GRCm39)	V205A	probably damaging	Het
Nr3c2	A	T	8: 77,635,438 (GRCm39)	I180F	possibly damaging	Het
Nup50l	A	T	6: 96,141,793 (GRCm39)	M417K	probably damaging	Het
Nup62	T	A	7: 44,478,289 (GRCm39)	S101R	probably damaging	Het
Or13c7	T	C	4: 43,854,890 (GRCm39)	S194P	probably damaging	Het
Or1l8	G	T	2: 36,818,024 (GRCm39)	T34K	possibly damaging	Het
Or4c3	A	G	2: 89,851,987 (GRCm39)	V141A	probably benign	Het
Or51b17	T	A	7: 103,542,862 (GRCm39)	I27F	probably benign	Het
Or56b2j	T	A	7: 104,353,068 (GRCm39)	I98N	probably damaging	Het
Or5b111	A	T	19: 13,291,034 (GRCm39)	I205N	probably benign	Het
Or5b124	A	T	19: 13,611,391 (GRCm39)	K305N	probably benign	Het
Or5p80	A	T	7: 108,229,819 (GRCm39)	I207F	probably damaging	Het
Osbpl6	A	T	2: 76,379,883 (GRCm39)	I158F	probably damaging	Het
Pcbp4	A	G	9: 106,339,301 (GRCm39)	T103A	probably benign	Het
Pcdha2	T	A	18: 37,073,953 (GRCm39)	L528Q	probably damaging	Het
Pcdhga5	T	C	18: 37,827,680 (GRCm39)	S43P	probably damaging	Het
Pdgfra	C	A	5: 75,349,973 (GRCm39)	N952K	probably benign	Het
Pla2r1	A	G	2: 60,365,328 (GRCm39)	S81P	probably damaging	Het
Rabggtb	A	T	3: 153,617,568 (GRCm39)	D43E	possibly damaging	Het
Rexo5	T	A	7: 119,424,774 (GRCm39)	C43*	probably null	Het
Robo1	T	A	16: 72,701,639 (GRCm39)	D168E	probably damaging	Het
Saxo4	A	T	19: 10,451,865 (GRCm39)	*428R	probably null	Het
Scimp	A	G	11: 70,688,865 (GRCm39)	M49T	unknown	Het
Sema3e	T	A	5: 14,275,579 (GRCm39)	V228E	probably damaging	Het
Sema6d	A	G	2: 124,498,738 (GRCm39)		probably null	Het
Serpinb6d	A	T	13: 33,850,428 (GRCm39)	D85V	possibly damaging	Het
Slc24a2	C	T	4: 86,909,745 (GRCm39)	V658I	probably damaging	Het
Snd1	T	C	6: 28,526,911 (GRCm39)	I198T	possibly damaging	Het
Snx33	C	T	9: 56,833,464 (GRCm39)	V202M	probably damaging	Het
Srcap	A	G	7: 127,121,189 (GRCm39)	E174G	probably damaging	Het
Srgap2	C	A	1: 131,220,314 (GRCm39)		probably null	Het
Stxbp5	A	T	10: 9,688,085 (GRCm39)	Y405*	probably null	Het
Thsd4	C	T	9: 59,895,320 (GRCm39)	R710H	probably benign	Het
Trp53inp1	T	A	4: 11,165,130 (GRCm39)	D51E	probably benign	Het
Ttc41	A	G	10: 86,566,882 (GRCm39)	D516G	probably benign	Het
Tut7	A	T	13: 59,937,266 (GRCm39)	L775H	probably damaging	Het
Uap1l1	A	G	2: 25,252,840 (GRCm39)	V400A	probably damaging	Het
Vit	T	C	17: 78,932,182 (GRCm39)	S430P	probably damaging	Het
Vmn1r9	T	A	6: 57,048,294 (GRCm39)	M123K	possibly damaging	Het
Vmn2r14	A	T	5: 109,369,384 (GRCm39)		probably null	Het
Vwa3a	A	G	7: 120,390,924 (GRCm39)	T746A	probably benign	Het
Wdfy4	G	T	14: 32,710,852 (GRCm39)	Y2578*	probably null	Het
Wdr53	A	T	16: 32,075,796 (GRCm39)	K334*	probably null	Het
Xpot	G	T	10: 121,442,713 (GRCm39)	H495Q	probably damaging	Het
Zfp109	T	C	7: 23,928,570 (GRCm39)	T288A	probably benign	Het
Zfp277	T	A	12: 40,413,152 (GRCm39)	E276V	probably damaging	Het
Zfp703	A	G	8: 27,468,729 (GRCm39)	D131G	probably benign	Het
Zfp985	T	A	4: 147,667,801 (GRCm39)	I223N	probably benign	Het
Zrsr2-ps1	T	C	11: 22,923,805 (GRCm39)	V193A	possibly damaging	Het
Zscan20	A	G	4: 128,481,958 (GRCm39)	I568T	possibly damaging	Het

Other mutations in Col27a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01305:Col27a1	APN	4	63,218,978 (GRCm39)	splice site	probably benign
IGL01461:Col27a1	APN	4	63,142,480 (GRCm39)	missense	probably damaging	1.00
IGL01534:Col27a1	APN	4	63,144,019 (GRCm39)	missense	probably benign	0.12
IGL01738:Col27a1	APN	4	63,182,016 (GRCm39)	splice site	probably benign
IGL01810:Col27a1	APN	4	63,143,868 (GRCm39)	missense	probably benign	0.21
IGL02127:Col27a1	APN	4	63,143,379 (GRCm39)	missense	possibly damaging	0.60
IGL02290:Col27a1	APN	4	63,144,163 (GRCm39)	missense	probably damaging	1.00
IGL02374:Col27a1	APN	4	63,211,486 (GRCm39)	missense	possibly damaging	0.86
IGL02548:Col27a1	APN	4	63,236,492 (GRCm39)	splice site	probably benign
IGL02792:Col27a1	APN	4	63,233,820 (GRCm39)	missense	unknown
IGL02931:Col27a1	APN	4	63,249,663 (GRCm39)	utr 3 prime	probably benign
IGL03107:Col27a1	APN	4	63,242,869 (GRCm39)	splice site	probably benign
IGL03121:Col27a1	APN	4	63,143,446 (GRCm39)	missense	probably benign	0.26
IGL03334:Col27a1	APN	4	63,232,959 (GRCm39)	missense	probably damaging	1.00
R0005:Col27a1	UTSW	4	63,143,637 (GRCm39)	missense	probably benign	0.04
R0025:Col27a1	UTSW	4	63,194,214 (GRCm39)	missense	probably damaging	1.00
R0141:Col27a1	UTSW	4	63,183,870 (GRCm39)	critical splice acceptor site	probably null
R0196:Col27a1	UTSW	4	63,142,503 (GRCm39)	missense	probably benign	0.02
R0359:Col27a1	UTSW	4	63,232,964 (GRCm39)	critical splice donor site	probably null
R0375:Col27a1	UTSW	4	63,143,898 (GRCm39)	missense	probably benign	0.23
R0432:Col27a1	UTSW	4	63,143,848 (GRCm39)	missense	possibly damaging	0.87
R0499:Col27a1	UTSW	4	63,218,978 (GRCm39)	splice site	probably benign
R0786:Col27a1	UTSW	4	63,209,815 (GRCm39)	critical splice donor site	probably null
R0891:Col27a1	UTSW	4	63,223,420 (GRCm39)	critical splice acceptor site	probably null
R1239:Col27a1	UTSW	4	63,237,152 (GRCm39)	splice site	probably benign
R1297:Col27a1	UTSW	4	63,183,868 (GRCm39)	splice site	probably benign
R1299:Col27a1	UTSW	4	63,183,868 (GRCm39)	splice site	probably benign
R1322:Col27a1	UTSW	4	63,246,803 (GRCm39)	utr 3 prime	probably benign
R1342:Col27a1	UTSW	4	63,175,351 (GRCm39)	critical splice donor site	probably null
R1446:Col27a1	UTSW	4	63,143,040 (GRCm39)	missense	probably damaging	1.00
R1629:Col27a1	UTSW	4	63,248,100 (GRCm39)	utr 3 prime	probably benign
R1644:Col27a1	UTSW	4	63,246,868 (GRCm39)	utr 3 prime	probably benign
R1774:Col27a1	UTSW	4	63,143,950 (GRCm39)	missense	probably damaging	1.00
R1807:Col27a1	UTSW	4	63,249,586 (GRCm39)	utr 3 prime	probably benign
R1952:Col27a1	UTSW	4	63,202,130 (GRCm39)	splice site	probably null
R1957:Col27a1	UTSW	4	63,196,031 (GRCm39)	missense	probably benign	0.03
R1970:Col27a1	UTSW	4	63,191,354 (GRCm39)	splice site	probably benign
R2164:Col27a1	UTSW	4	63,143,661 (GRCm39)	missense	probably benign	0.21
R3774:Col27a1	UTSW	4	63,232,963 (GRCm39)	missense	probably benign	0.00
R4078:Col27a1	UTSW	4	63,142,669 (GRCm39)	missense	probably damaging	1.00
R4353:Col27a1	UTSW	4	63,143,868 (GRCm39)	missense	probably benign	0.21
R4611:Col27a1	UTSW	4	63,211,743 (GRCm39)	missense	probably damaging	1.00
R4708:Col27a1	UTSW	4	63,202,150 (GRCm39)	missense	probably benign	0.01
R5149:Col27a1	UTSW	4	63,249,664 (GRCm39)	utr 3 prime	probably benign
R5411:Col27a1	UTSW	4	63,142,902 (GRCm39)	missense	probably damaging	1.00
R5451:Col27a1	UTSW	4	63,143,476 (GRCm39)	missense	probably damaging	0.98
R5615:Col27a1	UTSW	4	63,199,351 (GRCm39)	missense	probably damaging	0.96
R5657:Col27a1	UTSW	4	63,143,547 (GRCm39)	missense	probably damaging	0.97
R5838:Col27a1	UTSW	4	63,143,765 (GRCm39)	missense	probably damaging	1.00
R6230:Col27a1	UTSW	4	63,142,519 (GRCm39)	missense	probably damaging	1.00
R6326:Col27a1	UTSW	4	63,242,678 (GRCm39)	utr 3 prime	probably benign
R6457:Col27a1	UTSW	4	63,237,701 (GRCm39)	utr 3 prime	probably benign
R6624:Col27a1	UTSW	4	63,143,248 (GRCm39)	missense	probably benign	0.00
R6792:Col27a1	UTSW	4	63,235,740 (GRCm39)	missense	unknown
R6848:Col27a1	UTSW	4	63,220,608 (GRCm39)	missense	probably benign
R6962:Col27a1	UTSW	4	63,237,738 (GRCm39)	utr 3 prime	probably benign
R7053:Col27a1	UTSW	4	63,251,404 (GRCm39)	utr 3 prime	probably benign
R7206:Col27a1	UTSW	4	63,153,583 (GRCm39)	missense	probably benign	0.29
R7586:Col27a1	UTSW	4	63,143,278 (GRCm39)	missense	probably damaging	1.00
R7698:Col27a1	UTSW	4	63,143,955 (GRCm39)	missense	possibly damaging	0.78
R7714:Col27a1	UTSW	4	63,242,723 (GRCm39)	critical splice donor site	probably null
R7916:Col27a1	UTSW	4	63,142,789 (GRCm39)	missense	probably damaging	1.00
R7943:Col27a1	UTSW	4	63,236,520 (GRCm39)	missense	unknown
R7988:Col27a1	UTSW	4	63,249,559 (GRCm39)	missense	unknown
R8136:Col27a1	UTSW	4	63,202,190 (GRCm39)	missense	probably benign	0.06
R8243:Col27a1	UTSW	4	63,144,120 (GRCm39)	missense	probably damaging	1.00
R8245:Col27a1	UTSW	4	63,144,040 (GRCm39)	missense	probably damaging	0.97
R8350:Col27a1	UTSW	4	63,248,134 (GRCm39)	missense	unknown
R8437:Col27a1	UTSW	4	63,237,701 (GRCm39)	utr 3 prime	probably benign
R8450:Col27a1	UTSW	4	63,248,134 (GRCm39)	missense	unknown
R8542:Col27a1	UTSW	4	63,239,662 (GRCm39)	splice site	probably null
R8745:Col27a1	UTSW	4	63,144,153 (GRCm39)	missense	probably benign	0.02
R8821:Col27a1	UTSW	4	63,143,148 (GRCm39)	missense	probably benign	0.04
R8951:Col27a1	UTSW	4	63,191,311 (GRCm39)	missense	possibly damaging	0.92
R8970:Col27a1	UTSW	4	63,134,105 (GRCm39)	missense	unknown
R9115:Col27a1	UTSW	4	63,231,974 (GRCm39)	missense	unknown
R9185:Col27a1	UTSW	4	63,246,887 (GRCm39)	missense	unknown
R9291:Col27a1	UTSW	4	63,142,539 (GRCm39)	missense	probably damaging	0.99
R9404:Col27a1	UTSW	4	63,194,178 (GRCm39)	missense	possibly damaging	0.93
Z1176:Col27a1	UTSW	4	63,144,025 (GRCm39)	missense	probably damaging	0.99
Z1177:Col27a1	UTSW	4	63,199,526 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GATCCCTTTAGAGCCAAGGC -3'
(R):5'- CTACCAGCGGTGTATGAAAGAAC -3'

Sequencing Primer
(F):5'- CTGCTGCTGGCCACAAGAG -3'
(R):5'- GCCTCAAAAGGACAGTCTGG -3'

Posted On

2016-03-17