Mutagenetix > Incidental Mutation

Incidental Mutation 'R5558:Or10j7'

436378

Institutional Source

Beutler Lab

Gene Symbol

Or10j7

Ensembl Gene

ENSMUSG00000058981

Gene Name

olfactory receptor family 10 subfamily J member 7

Synonyms

GA_x6K02T2R7CC-664297-665229, MOR267-5, Olfr1406

MMRRC Submission

043115-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.081) question?

Stock #

R5558 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

173011009-173012071 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 173011585 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Glycine at position 139 (R139G)

Ref Sequence

ENSEMBL: ENSMUSP00000151023 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072395] [ENSMUST00000201132] [ENSMUST00000215878]

AlphaFold

E9Q8X1

Predicted Effect

probably benign
Transcript: ENSMUST00000072395
AA Change: R139G

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000072231
Gene: ENSMUSG00000058981
AA Change: R139G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	307	3.9e-55	PFAM
Pfam:7tm_1	41	289	2.7e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000201132
AA Change: R139G

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000144530
Gene: ENSMUSG00000058981
AA Change: R139G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	88	5.4e-11	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215878
AA Change: R139G

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcg4	T	C	9: 44,192,705 (GRCm39)	K177E	probably damaging	Het
Acvr1	T	C	2: 58,349,029 (GRCm39)	T378A	probably damaging	Het
Agtpbp1	A	G	13: 59,630,394 (GRCm39)	V779A	probably benign	Het
Alms1	C	T	6: 85,618,311 (GRCm39)	Q2786*	probably null	Het
Arhgef28	A	C	13: 98,097,968 (GRCm39)	L882R	probably damaging	Het
Cbx2	A	G	11: 118,919,775 (GRCm39)	T447A	probably benign	Het
Ch25h	A	G	19: 34,451,863 (GRCm39)	W222R	probably damaging	Het
Chsy3	T	G	18: 59,309,469 (GRCm39)	S241A	probably damaging	Het
Cyfip1	T	C	7: 55,541,749 (GRCm39)	S345P	possibly damaging	Het
Cyp4a30b	C	A	4: 115,316,063 (GRCm39)	D291E	probably damaging	Het
Dcstamp	A	G	15: 39,622,936 (GRCm39)	Y419C	probably damaging	Het
Eif3d	G	A	15: 77,846,047 (GRCm39)	R359C	probably damaging	Het
Elavl4	C	A	4: 110,063,800 (GRCm39)	G267V	probably benign	Het
Gars1	T	C	6: 55,042,592 (GRCm39)	S442P	probably damaging	Het
Gas6	C	T	8: 13,516,764 (GRCm39)	R578Q	probably null	Het
H4c4	C	A	13: 23,765,779 (GRCm39)	N65K	possibly damaging	Het
Ifitm6	T	C	7: 140,595,985 (GRCm39)	I103V	probably benign	Het
Igsf5	T	A	16: 96,187,731 (GRCm39)	I241N	possibly damaging	Het
Iqce	A	G	5: 140,657,560 (GRCm39)		probably null	Het
Khdc4	A	C	3: 88,600,403 (GRCm39)	H164P	probably damaging	Het
Kif20b	C	A	19: 34,928,949 (GRCm39)	Q1192K	probably damaging	Het
Lap3	A	G	5: 45,662,093 (GRCm39)	N298D	probably benign	Het
Lca5	A	T	9: 83,283,796 (GRCm39)	S246T	probably damaging	Het
Mrps5	G	A	2: 127,444,355 (GRCm39)	G330S	probably damaging	Het
Myh15	C	T	16: 48,889,900 (GRCm39)	R164C	probably benign	Het
Myo19	C	G	11: 84,801,274 (GRCm39)	P940R	probably damaging	Het
Or5b12b	T	C	19: 12,861,751 (GRCm39)	F169L	probably benign	Het
Pcdh10	A	G	3: 45,338,603 (GRCm39)	D920G	probably damaging	Het
Pgm5	A	T	19: 24,801,815 (GRCm39)		probably null	Het
Phactr4	T	C	4: 132,105,766 (GRCm39)	E137G	probably damaging	Het
Pkn1	C	T	8: 84,411,351 (GRCm39)	V239M	probably damaging	Het
Plcz1	T	A	6: 139,985,481 (GRCm39)	D20V	probably damaging	Het
Prkca	A	T	11: 107,872,473 (GRCm39)	I429N	probably damaging	Het
Ptk2	A	T	15: 73,176,294 (GRCm39)	Y251N	probably damaging	Het
R3hdm2	T	C	10: 127,280,271 (GRCm39)	F31S	probably damaging	Het
Rab11fip1	T	A	8: 27,642,003 (GRCm39)	E932V	probably damaging	Het
Rilp	A	G	11: 75,402,251 (GRCm39)	Y250C	probably damaging	Het
Sclt1	A	G	3: 41,616,025 (GRCm39)	I474T	probably benign	Het
Slc14a2	T	A	18: 78,202,381 (GRCm39)	D583V	possibly damaging	Het
Slc30a4	A	G	2: 122,528,903 (GRCm39)	I324T	probably damaging	Het
Slc6a5	G	A	7: 49,577,321 (GRCm39)	V326I	probably benign	Het
Slco1c1	C	T	6: 141,513,222 (GRCm39)	T617I	probably damaging	Het
Sntg1	A	G	1: 8,484,495 (GRCm39)	S442P	possibly damaging	Het
Sys1	G	T	2: 164,306,429 (GRCm39)	A128S	possibly damaging	Het
Tbc1d2	C	T	4: 46,629,912 (GRCm39)	G252R	probably benign	Het
Tmed5	A	G	5: 108,272,462 (GRCm39)	I212T	probably benign	Het
Tnks	T	C	8: 35,432,819 (GRCm39)	M1V	probably null	Het
Trpa1	T	C	1: 14,968,492 (GRCm39)	H452R	probably damaging	Het
Trpm3	T	C	19: 22,955,937 (GRCm39)	V1133A	probably damaging	Het
Ttn	T	A	2: 76,555,395 (GRCm39)	I30537F	probably damaging	Het
Ush2a	T	C	1: 188,530,024 (GRCm39)	V3271A	possibly damaging	Het
Utp20	C	G	10: 88,587,329 (GRCm39)	G2489R	probably damaging	Het
Vmn1r70	A	T	7: 10,368,402 (GRCm39)	S278C	probably benign	Het
Wdpcp	G	A	11: 21,661,732 (GRCm39)	A335T	probably benign	Het
Zc3h18	G	T	8: 123,113,659 (GRCm39)	R217L	probably damaging	Het
Zfp384	ACAGCAGCAGCAGCAGCAGCAGC	ACAGCAGCAGCAGCAGCAGC	6: 125,013,472 (GRCm39)		probably benign	Het
Zfp955b	C	T	17: 33,521,161 (GRCm39)	T210I	possibly damaging	Het

Other mutations in Or10j7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00946:Or10j7	APN	1	173,011,190 (GRCm39)	missense	probably benign	0.45
IGL01609:Or10j7	APN	1	173,011,843 (GRCm39)	missense	probably benign	0.00
IGL01996:Or10j7	APN	1	173,011,294 (GRCm39)	missense	probably benign	0.00
F5770:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05
PIT4378001:Or10j7	UTSW	1	173,011,381 (GRCm39)	missense	probably benign	0.00
R0053:Or10j7	UTSW	1	173,011,845 (GRCm39)	missense	probably benign	0.35
R0800:Or10j7	UTSW	1	173,011,627 (GRCm39)	missense	probably damaging	1.00
R1793:Or10j7	UTSW	1	173,011,976 (GRCm39)	missense	probably benign
R2230:Or10j7	UTSW	1	173,011,182 (GRCm39)	missense	probably benign	0.04
R2232:Or10j7	UTSW	1	173,011,182 (GRCm39)	missense	probably benign	0.04
R5395:Or10j7	UTSW	1	173,011,247 (GRCm39)	nonsense	probably null
R5455:Or10j7	UTSW	1	173,011,818 (GRCm39)	missense	probably damaging	1.00
R5457:Or10j7	UTSW	1	173,011,180 (GRCm39)	missense	probably damaging	1.00
R5760:Or10j7	UTSW	1	173,011,318 (GRCm39)	missense	probably benign	0.05
R6285:Or10j7	UTSW	1	173,011,477 (GRCm39)	missense	probably damaging	1.00
R7159:Or10j7	UTSW	1	173,011,890 (GRCm39)	missense	possibly damaging	0.95
R7676:Or10j7	UTSW	1	173,011,120 (GRCm39)	nonsense	probably null
R8700:Or10j7	UTSW	1	173,011,429 (GRCm39)	missense	probably benign
R8829:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
R9721:Or10j7	UTSW	1	173,011,915 (GRCm39)	missense	probably benign	0.07
R9785:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
R9788:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
R9789:Or10j7	UTSW	1	173,011,458 (GRCm39)	missense	probably benign	0.06
V7580:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05
V7581:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05
V7582:Or10j7	UTSW	1	173,011,531 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- CAGGGGCTGTGATGACCATAAG -3'
(R):5'- ATCATCCCACGCATGCTTGG -3'

Sequencing Primer
(F):5'- TGACCATAAGGCTAAGAGCTAACGTC -3'
(R):5'- CATGCTTGGCAGCCTTGTAG -3'

Posted On

2016-10-24