Mutagenetix > Incidental Mutation

Incidental Mutation 'R5778:Or7d10'

446827

Institutional Source

Beutler Lab

Gene Symbol

Or7d10

Ensembl Gene

ENSMUSG00000051118

Gene Name

olfactory receptor family 7 subfamily D member 10

Synonyms

18A, Olfr77, GA_x6K02T2PVTD-13660026-13660964, MOR143-1

MMRRC Submission

043376-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.076) question?

Stock #

R5778 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

19828525-19833806 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 19832337 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Isoleucine at position 277 (M277I)

Ref Sequence

ENSEMBL: ENSMUSP00000149055 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000057596] [ENSMUST00000217347]

AlphaFold

Q8VEY9

Predicted Effect

probably benign
Transcript: ENSMUST00000057596
AA Change: M277I

PolyPhen 2 Score 0.196 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000058810
Gene: ENSMUSG00000051118
AA Change: M277I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	3e-53	PFAM
Pfam:7tm_1	41	290	1.2e-24	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000217347
AA Change: M277I

PolyPhen 2 Score 0.196 (Sensitivity: 0.92; Specificity: 0.87)

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acta1	A	G	8: 124,618,864 (GRCm39)	S340P	probably benign	Het
Anpep	T	C	7: 79,486,139 (GRCm39)	T528A	probably benign	Het
Apob	T	A	12: 8,065,074 (GRCm39)	D4014E	probably benign	Het
Atp23	A	G	10: 126,735,451 (GRCm39)	C78R	probably damaging	Het
Atp8b3	A	G	10: 80,356,007 (GRCm39)	F1235S	probably benign	Het
B3gnt3	T	C	8: 72,145,582 (GRCm39)	D262G	probably benign	Het
Bltp1	A	T	3: 37,012,863 (GRCm39)	I1848F	probably damaging	Het
Brca1	G	A	11: 101,416,127 (GRCm39)	A669V	possibly damaging	Het
Caprin2	A	T	6: 148,770,820 (GRCm39)	S391R	probably benign	Het
Cdh15	G	A	8: 123,583,326 (GRCm39)	R43Q	possibly damaging	Het
Celsr1	A	T	15: 85,917,156 (GRCm39)	N272K	probably damaging	Het
Cryzl2	T	G	1: 157,298,357 (GRCm39)	S249A	probably benign	Het
Dsg1b	A	T	18: 20,542,279 (GRCm39)	T929S	possibly damaging	Het
Dusp16	G	T	6: 134,695,277 (GRCm39)	T518N	probably benign	Het
Eno2	A	T	6: 124,743,261 (GRCm39)	H158Q	probably damaging	Het
Ep400	T	C	5: 110,867,450 (GRCm39)	D954G	unknown	Het
Erg28	T	C	12: 85,866,254 (GRCm39)	T75A	possibly damaging	Het
Fam135b	T	A	15: 71,350,881 (GRCm39)	T332S	probably damaging	Het
Fam221b	A	G	4: 43,660,683 (GRCm39)	F357L	probably damaging	Het
Fcgr2b	C	T	1: 170,790,957 (GRCm39)	G279R	probably damaging	Het
Fryl	A	T	5: 73,230,121 (GRCm39)	L1679M	probably damaging	Het
Galm	T	A	17: 80,435,146 (GRCm39)	M1K	probably null	Het
Gsg1	A	T	6: 135,221,348 (GRCm39)	I17N	possibly damaging	Het
Hif3a	A	T	7: 16,785,909 (GRCm39)	I129N	probably damaging	Het
Ighv1-11	A	T	12: 114,576,051 (GRCm39)	W55R	probably damaging	Het
Ighv1-20	C	A	12: 114,687,497 (GRCm39)	K82N	probably benign	Het
Igsf21	C	T	4: 139,764,832 (GRCm39)	E148K	probably benign	Het
Il22b	C	A	10: 118,130,768 (GRCm39)	E43*	probably null	Het
Klhdc7b	A	T	15: 89,271,523 (GRCm39)	R802W	probably damaging	Het
Krt8	T	C	15: 101,912,374 (GRCm39)	I101V	probably damaging	Het
Lrrc7	A	G	3: 157,876,380 (GRCm39)	L570P	probably damaging	Het
Map4k1	C	G	7: 28,693,646 (GRCm39)	N412K	probably benign	Het
Metrnl	A	T	11: 121,605,564 (GRCm39)	I118F	possibly damaging	Het
Mmp12	T	A	9: 7,350,106 (GRCm39)	D202E	probably damaging	Het
Mpp2	A	T	11: 101,955,269 (GRCm39)	S119T	probably benign	Het
Ncoa6	T	C	2: 155,248,688 (GRCm39)	T1539A	probably benign	Het
Nlrc5	A	G	8: 95,206,154 (GRCm39)	T715A	possibly damaging	Het
Nsd1	T	A	13: 55,454,792 (GRCm39)	N1963K	probably damaging	Het
Nsd3	T	C	8: 26,149,834 (GRCm39)	Y340H	probably damaging	Het
Nwd1	T	A	8: 73,419,745 (GRCm39)	S977T	probably damaging	Het
Or5d39	T	A	2: 87,979,961 (GRCm39)	Y134F	probably damaging	Het
Pcdh8	T	C	14: 80,008,197 (GRCm39)	E122G	probably damaging	Het
Pcmtd2	A	C	2: 181,496,991 (GRCm39)	T323P	probably benign	Het
Pign	C	A	1: 105,519,447 (GRCm39)	G492C	probably damaging	Het
Plppr3	A	G	10: 79,702,337 (GRCm39)	V245A	possibly damaging	Het
Prl7a2	C	T	13: 27,844,983 (GRCm39)	W134*	probably null	Het
Prom1	A	T	5: 44,164,389 (GRCm39)	N722K	probably benign	Het
Rasal2	A	T	1: 156,988,860 (GRCm39)	N663K	probably damaging	Het
Rgl1	G	T	1: 152,428,172 (GRCm39)	H315Q	probably benign	Het
Smpdl3a	C	A	10: 57,677,097 (GRCm39)	A65E	probably damaging	Het
Spdye4b	G	A	5: 143,188,142 (GRCm39)	D212N	probably damaging	Het
Tafa3	T	A	3: 104,679,505 (GRCm39)	K126N	probably damaging	Het
Tanc1	G	T	2: 59,529,691 (GRCm39)		probably null	Het
Trio	A	G	15: 27,856,250 (GRCm39)	V706A	probably benign	Het
Tshz1	T	A	18: 84,033,805 (GRCm39)	Q201L	probably damaging	Het
Ubqln1	C	T	13: 58,331,131 (GRCm39)	M365I	probably benign	Het
Usp42	A	T	5: 143,705,331 (GRCm39)	Y383N	probably damaging	Het
Vmn1r189	A	G	13: 22,286,552 (GRCm39)	I95T	probably damaging	Het
Vmn2r49	A	T	7: 9,710,274 (GRCm39)	S819R	probably damaging	Het

Other mutations in Or7d10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01152:Or7d10	APN	9	19,832,245 (GRCm39)	missense	possibly damaging	0.87
IGL01318:Or7d10	APN	9	19,832,054 (GRCm39)	missense	probably benign	0.44
IGL01547:Or7d10	APN	9	19,832,197 (GRCm39)	missense	probably benign	0.00
IGL01635:Or7d10	APN	9	19,831,780 (GRCm39)	missense	probably damaging	1.00
IGL02112:Or7d10	APN	9	19,831,821 (GRCm39)	missense	possibly damaging	0.48
IGL02858:Or7d10	APN	9	19,831,747 (GRCm39)	missense	probably damaging	0.99
IGL02904:Or7d10	APN	9	19,832,393 (GRCm39)	missense	probably damaging	1.00
IGL02956:Or7d10	APN	9	19,832,348 (GRCm39)	missense	possibly damaging	0.87
IGL03066:Or7d10	APN	9	19,831,667 (GRCm39)	missense	probably benign	0.12
R0662:Or7d10	UTSW	9	19,831,796 (GRCm39)	missense	probably damaging	1.00
R1222:Or7d10	UTSW	9	19,832,344 (GRCm39)	missense	possibly damaging	0.87
R1572:Or7d10	UTSW	9	19,832,208 (GRCm39)	missense	probably benign	0.35
R1761:Or7d10	UTSW	9	19,832,445 (GRCm39)	makesense	probably null
R2409:Or7d10	UTSW	9	19,832,077 (GRCm39)	missense	probably damaging	1.00
R2409:Or7d10	UTSW	9	19,832,072 (GRCm39)	missense	probably benign	0.31
R3621:Or7d10	UTSW	9	19,832,209 (GRCm39)	missense	probably damaging	0.99
R3849:Or7d10	UTSW	9	19,832,105 (GRCm39)	missense	probably damaging	1.00
R3850:Or7d10	UTSW	9	19,832,105 (GRCm39)	missense	probably damaging	1.00
R4277:Or7d10	UTSW	9	19,831,685 (GRCm39)	missense	possibly damaging	0.91
R4768:Or7d10	UTSW	9	19,831,841 (GRCm39)	missense	possibly damaging	0.56
R4979:Or7d10	UTSW	9	19,831,655 (GRCm39)	missense	probably benign	0.03
R5276:Or7d10	UTSW	9	19,831,917 (GRCm39)	missense	possibly damaging	0.87
R5503:Or7d10	UTSW	9	19,831,675 (GRCm39)	missense	probably benign	0.36
R5760:Or7d10	UTSW	9	19,832,050 (GRCm39)	missense	probably benign	0.00
R5930:Or7d10	UTSW	9	19,832,206 (GRCm39)	missense	probably damaging	0.99
R6012:Or7d10	UTSW	9	19,832,237 (GRCm39)	missense	probably damaging	0.99
R7269:Or7d10	UTSW	9	19,831,631 (GRCm39)	missense	possibly damaging	0.95
R7977:Or7d10	UTSW	9	19,831,610 (GRCm39)	missense	possibly damaging	0.48
R7987:Or7d10	UTSW	9	19,831,610 (GRCm39)	missense	possibly damaging	0.48
R8174:Or7d10	UTSW	9	19,832,020 (GRCm39)	missense	probably damaging	1.00
Z1088:Or7d10	UTSW	9	19,832,008 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACTGCAGTGCTAGGTATGTTTCC -3'
(R):5'- TAGAAACTTCAAAGATCCAGGGATG -3'

Sequencing Primer
(F):5'- TCCTGTCATTGGGATAGCTTTC -3'
(R):5'- GATCCAGGGATGAAAAGACTTTG -3'

Posted On

2016-12-15