Mutagenetix > Incidental Mutation

Incidental Mutation 'R6197:Vnn3'

502988

Institutional Source

Beutler Lab

Gene Symbol

Vnn3

Ensembl Gene

ENSMUSG00000020010

Gene Name

vanin 3

Synonyms

MMRRC Submission

044337-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.055) question?

Stock #

R6197 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23727360-23745741 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 23732187 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Serine at position 146 (C146S)

Ref Sequence

ENSEMBL: ENSMUSP00000020190 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020190]

AlphaFold

Q9QZ25

Predicted Effect

probably damaging
Transcript: ENSMUST00000020190
AA Change: C146S

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000020190
Gene: ENSMUSG00000020010
AA Change: C146S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:CN_hydrolase	51	296	6.9e-19	PFAM

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.6%
20x: 98.7%

Validation Efficiency

92% (49/53)

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700109H08Rik	T	A	5: 3,630,442 (GRCm39)	Y119*	probably null	Het
Abcb9	C	A	5: 124,209,812 (GRCm39)	E664*	probably null	Het
Adgrl2	T	C	3: 148,564,578 (GRCm39)	D334G	probably damaging	Het
Ak9	T	G	10: 41,193,826 (GRCm39)	C57G	probably damaging	Het
Als2cl	A	G	9: 110,724,952 (GRCm39)	K809E	probably damaging	Het
Arsi	G	A	18: 61,049,723 (GRCm39)	G202E	probably benign	Het
Atp11a	A	G	8: 12,896,099 (GRCm39)	I223V	probably benign	Het
Cacna2d3	A	T	14: 28,630,278 (GRCm39)	V1022E	probably benign	Het
Cc2d1b	C	T	4: 108,490,422 (GRCm39)	R825W	probably damaging	Het
Cd37	A	G	7: 44,886,598 (GRCm39)	C85R	probably damaging	Het
Ces1g	T	C	8: 94,063,764 (GRCm39)	S7G	probably benign	Het
Cltc	T	C	11: 86,611,188 (GRCm39)	N561S	probably benign	Het
Cnbd2	A	G	2: 156,217,494 (GRCm39)	E661G	possibly damaging	Het
Col6a3	A	T	1: 90,750,063 (GRCm39)	F257Y	probably damaging	Het
Cpt1b	T	C	15: 89,309,037 (GRCm39)	Y55C	possibly damaging	Het
Csmd1	A	G	8: 15,976,611 (GRCm39)	V2869A	probably benign	Het
Dnah11	T	C	12: 118,143,482 (GRCm39)	E387G	probably benign	Het
Eny2	T	A	15: 44,292,949 (GRCm39)		probably null	Het
Fbxo10	G	T	4: 45,043,857 (GRCm39)	H655Q	probably benign	Het
Gm14403	AAACCCTA	AA	2: 177,201,448 (GRCm39)		probably benign	Het
Gpx2	C	T	12: 76,842,068 (GRCm39)	G28S	probably damaging	Het
H2-Q5	G	T	17: 35,613,918 (GRCm39)	A156S	probably benign	Het
Hoxd4	A	G	2: 74,558,807 (GRCm39)	D210G	possibly damaging	Het
Hoxd9	A	G	2: 74,529,166 (GRCm39)	Q256R	probably damaging	Het
Macf1	A	G	4: 123,346,085 (GRCm39)	V2571A	probably damaging	Het
Mtus1	A	G	8: 41,537,074 (GRCm39)	V214A	possibly damaging	Het
Myh1	C	T	11: 67,111,793 (GRCm39)	A1716V	probably benign	Het
Nbeal1	A	G	1: 60,261,287 (GRCm39)	D249G	probably damaging	Het
Nup54	TCTGCTGCTGCTGCTGCTGCTGCTG	TCTGCTGCTGCTGCTGCTGCTG	5: 92,578,663 (GRCm39)		probably benign	Het
Or10v9	G	T	19: 11,833,148 (GRCm39)	H56Q	probably damaging	Het
Or4c124	A	T	2: 89,155,677 (GRCm39)	N282K	probably damaging	Het
Or5w17	C	T	2: 87,583,696 (GRCm39)	V214I	probably benign	Het
Or7a37	T	G	10: 78,805,974 (GRCm39)	L164V	probably damaging	Het
Peg10	GC	GCTCC	6: 4,756,452 (GRCm39)		probably benign	Het
Pramel23	T	C	4: 143,423,886 (GRCm39)	Y301C	possibly damaging	Het
Prss59	A	G	6: 40,897,939 (GRCm39)	I248T	probably benign	Het
Pwwp2a	A	G	11: 43,595,423 (GRCm39)	D196G	probably benign	Het
Rarg	A	T	15: 102,150,327 (GRCm39)	C93S	possibly damaging	Het
Rcc1	T	C	4: 132,065,073 (GRCm39)	D150G	possibly damaging	Het
Ripk2	A	G	4: 16,163,330 (GRCm39)	Y23H	probably damaging	Het
Rragc	A	G	4: 123,811,340 (GRCm39)	Y5C	possibly damaging	Het
Rubcnl	G	A	14: 75,269,369 (GRCm39)	G9D	probably damaging	Het
Sephs2	A	T	7: 126,872,073 (GRCm39)	V340E	probably damaging	Het
Slc22a3	C	T	17: 12,677,438 (GRCm39)	M243I	probably benign	Het
Slco6c1	A	G	1: 97,000,518 (GRCm39)		probably null	Het
Sncaip	T	G	18: 53,039,966 (GRCm39)	I308R	probably damaging	Het
Supt16	G	A	14: 52,408,338 (GRCm39)	T869M	probably damaging	Het
Tanc1	G	A	2: 59,674,366 (GRCm39)	E1817K	possibly damaging	Het
Usp4	C	A	9: 108,248,154 (GRCm39)	Q395K	probably damaging	Het
Usp49	T	A	17: 47,984,272 (GRCm39)	S210T	possibly damaging	Het
Virma	A	G	4: 11,505,498 (GRCm39)	I124M	probably damaging	Het
Vmn1r234	G	A	17: 21,449,589 (GRCm39)	V168I	probably benign	Het
Ylpm1	A	G	12: 85,088,953 (GRCm39)	D1234G	probably damaging	Het
Zfp617	T	A	8: 72,687,098 (GRCm39)	V476E	probably benign	Het

Other mutations in Vnn3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00675:Vnn3	APN	10	23,743,066 (GRCm39)	missense	possibly damaging	0.77
IGL01300:Vnn3	APN	10	23,740,263 (GRCm39)	missense	possibly damaging	0.87
IGL01349:Vnn3	APN	10	23,727,814 (GRCm39)	missense	probably damaging	0.99
IGL02491:Vnn3	APN	10	23,741,816 (GRCm39)	missense	probably benign	0.11
IGL03256:Vnn3	APN	10	23,727,698 (GRCm39)	splice site	probably benign
IGL03289:Vnn3	APN	10	23,741,735 (GRCm39)	missense	possibly damaging	0.92
IGL02799:Vnn3	UTSW	10	23,727,869 (GRCm39)	missense	possibly damaging	0.64
R0599:Vnn3	UTSW	10	23,741,603 (GRCm39)	missense	possibly damaging	0.94
R1703:Vnn3	UTSW	10	23,741,828 (GRCm39)	missense	probably benign
R1753:Vnn3	UTSW	10	23,741,718 (GRCm39)	missense	probably benign	0.27
R2119:Vnn3	UTSW	10	23,740,311 (GRCm39)	missense	probably damaging	1.00
R2288:Vnn3	UTSW	10	23,740,354 (GRCm39)	missense	probably benign	0.03
R4255:Vnn3	UTSW	10	23,741,720 (GRCm39)	missense	probably benign	0.18
R4458:Vnn3	UTSW	10	23,741,567 (GRCm39)	missense	probably benign	0.23
R4518:Vnn3	UTSW	10	23,743,124 (GRCm39)	missense	possibly damaging	0.77
R4545:Vnn3	UTSW	10	23,732,224 (GRCm39)	missense	probably benign	0.00
R4723:Vnn3	UTSW	10	23,727,589 (GRCm39)	missense	possibly damaging	0.88
R4791:Vnn3	UTSW	10	23,740,519 (GRCm39)	missense	probably benign
R4921:Vnn3	UTSW	10	23,740,473 (GRCm39)	missense	probably benign	0.01
R5152:Vnn3	UTSW	10	23,740,237 (GRCm39)	missense	probably benign	0.01
R5390:Vnn3	UTSW	10	23,727,483 (GRCm39)	start codon destroyed	probably null	1.00
R5545:Vnn3	UTSW	10	23,742,992 (GRCm39)	missense	probably benign	0.00
R6751:Vnn3	UTSW	10	23,745,523 (GRCm39)	missense	probably benign	0.00
R6846:Vnn3	UTSW	10	23,727,620 (GRCm39)	missense	probably benign
R6917:Vnn3	UTSW	10	23,741,832 (GRCm39)	missense	possibly damaging	0.50
R7073:Vnn3	UTSW	10	23,740,311 (GRCm39)	missense	probably damaging	1.00
R7100:Vnn3	UTSW	10	23,741,840 (GRCm39)	missense	probably damaging	1.00
R7152:Vnn3	UTSW	10	23,727,513 (GRCm39)	missense	possibly damaging	0.88
R7336:Vnn3	UTSW	10	23,727,806 (GRCm39)	missense	probably benign	0.42
R7421:Vnn3	UTSW	10	23,741,666 (GRCm39)	missense	probably benign	0.06
R7875:Vnn3	UTSW	10	23,743,146 (GRCm39)	missense	possibly damaging	0.80
R8353:Vnn3	UTSW	10	23,745,443 (GRCm39)	missense	probably benign	0.01
R8453:Vnn3	UTSW	10	23,745,443 (GRCm39)	missense	probably benign	0.01
R8465:Vnn3	UTSW	10	23,741,780 (GRCm39)	missense	possibly damaging	0.67
R9105:Vnn3	UTSW	10	23,740,461 (GRCm39)	missense	probably damaging	1.00
R9261:Vnn3	UTSW	10	23,741,607 (GRCm39)	missense	probably damaging	0.97
R9441:Vnn3	UTSW	10	23,740,498 (GRCm39)	missense	possibly damaging	0.94
R9718:Vnn3	UTSW	10	23,745,454 (GRCm39)	nonsense	probably null
R9737:Vnn3	UTSW	10	23,741,813 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- GCCGATTCTTGAACTAGAGCTTTTG -3'
(R):5'- CACACAGAGAATCCTTAACTGGTATAC -3'

Sequencing Primer
(F):5'- TAGGTTTGGCTACACACC -3'
(R):5'- CTGGTATACAAATAAAGGAAATTGCC -3'

Posted On

2018-02-27