Mutagenetix > Incidental Mutation

Incidental Mutation 'R6480:Cdc14b'

517052

Institutional Source

Beutler Lab

Gene Symbol

Cdc14b

Ensembl Gene

ENSMUSG00000033102

Gene Name

CDC14 cell division cycle 14B

Synonyms

A530086E13Rik, 2810432N10Rik

MMRRC Submission

044612-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.240) question?

Stock #

R6480 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

64337082-64423104 bp(-) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

T to C at 64373464 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152388 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039318] [ENSMUST00000109769] [ENSMUST00000109770] [ENSMUST00000221139] [ENSMUST00000221634]

AlphaFold

Q6PFY9

Predicted Effect

probably null
Transcript: ENSMUST00000039318

SMART Domains

Protein: ENSMUSP00000046003
Gene: ENSMUSG00000033102

Domain	Start	End	E-Value	Type
low complexity region	15	34	N/A	INTRINSIC
Pfam:DSPn	51	189	6.1e-57	PFAM
Pfam:DSPc	240	365	9.2e-17	PFAM
Pfam:Y_phosphatase	244	365	1e-7	PFAM
transmembrane domain	445	467	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000109769

SMART Domains

Protein: ENSMUSP00000105391
Gene: ENSMUSG00000033102

Domain	Start	End	E-Value	Type
Pfam:DSPn	12	152	2.5e-58	PFAM
Pfam:DSPc	203	328	8e-17	PFAM
Pfam:Y_phosphatase	206	328	8.9e-8	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000109770

SMART Domains

Protein: ENSMUSP00000105392
Gene: ENSMUSG00000033102

Domain	Start	End	E-Value	Type
low complexity region	15	34	N/A	INTRINSIC
Pfam:DSPn	51	189	3.4e-57	PFAM
Pfam:DSPc	240	365	2.8e-16	PFAM
Pfam:Y_phosphatase	252	364	2.4e-7	PFAM
transmembrane domain	445	467	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000221139

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221437

Predicted Effect

probably null
Transcript: ENSMUST00000221634

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.8%
20x: 93.4%

Validation Efficiency

98% (57/58)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit premature aging, including premature cataracts and kyphosis; reduced fertility, particularly in female mice; and impaired contextual conditioning. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Arl8b	T	A	6: 108,792,010 (GRCm39)	N90K	possibly damaging	Het
Art3	T	C	5: 92,540,676 (GRCm39)	F140L	probably damaging	Het
Ascc3	T	A	10: 50,587,049 (GRCm39)	M967K	probably damaging	Het
Ccne1	G	A	7: 37,806,279 (GRCm39)		probably benign	Het
Ceacam2	T	A	7: 25,219,414 (GRCm39)	E282V	probably damaging	Het
Clk4	T	A	11: 51,161,373 (GRCm39)	C86*	probably null	Het
Cul2	A	C	18: 3,417,561 (GRCm39)	K115T	possibly damaging	Het
Dhx29	A	T	13: 113,090,322 (GRCm39)	K800*	probably null	Het
Dlec1	T	C	9: 118,976,758 (GRCm39)	F1741S	probably benign	Het
Dnah12	A	G	14: 26,594,412 (GRCm39)	T3455A	probably damaging	Het
Dnah8	G	A	17: 30,967,542 (GRCm39)	D2585N	probably benign	Het
Drd4	A	T	7: 140,874,706 (GRCm39)	I366F	possibly damaging	Het
Fam169b	T	A	7: 68,003,466 (GRCm39)	Y273*	probably null	Het
Fbn1	T	A	2: 125,177,338 (GRCm39)	Y1833F	probably benign	Het
Fsip2	A	G	2: 82,820,430 (GRCm39)	T5388A	possibly damaging	Het
Gjb6	T	C	14: 57,361,899 (GRCm39)	I121V	probably benign	Het
Glg1	T	C	8: 111,924,338 (GRCm39)	T217A	possibly damaging	Het
Igkv5-48	A	G	6: 69,703,810 (GRCm39)	S32P	probably benign	Het
Itgax	C	A	7: 127,747,771 (GRCm39)	F1062L	probably benign	Het
Lct	G	A	1: 128,222,057 (GRCm39)	T1494I	probably damaging	Het
Lilra6	G	A	7: 3,915,932 (GRCm39)	T309I	probably damaging	Het
Mical3	T	C	6: 121,011,236 (GRCm39)	T321A	possibly damaging	Het
Mmp23	T	A	4: 155,736,798 (GRCm39)	N104I	probably damaging	Het
Muc17	T	C	5: 137,171,238 (GRCm39)	Y131C		Het
Naip2	A	C	13: 100,298,549 (GRCm39)	S496A	probably benign	Het
Ndufs2	A	G	1: 171,064,267 (GRCm39)	S348P	probably damaging	Het
Nepro	T	C	16: 44,547,438 (GRCm39)	S52P	probably damaging	Het
Nlrp6	A	G	7: 140,507,356 (GRCm39)	E874G	possibly damaging	Het
Nsmce1	A	G	7: 125,090,590 (GRCm39)	V9A	probably benign	Het
Or1q1	T	C	2: 36,887,007 (GRCm39)	F62L	probably benign	Het
Or2a7	T	C	6: 43,151,000 (GRCm39)	F27L	probably benign	Het
Or5b105	T	A	19: 13,079,838 (GRCm39)	T277S	probably benign	Het
Or6c3	A	T	10: 129,308,590 (GRCm39)	I10F	possibly damaging	Het
Per2	A	T	1: 91,357,104 (GRCm39)		probably null	Het
Pgbd1	T	C	13: 21,607,646 (GRCm39)	I183V	probably benign	Het
Pik3c2g	T	C	6: 139,676,195 (GRCm39)	V113A	probably benign	Het
Pipox	A	G	11: 77,773,474 (GRCm39)	L259P	probably damaging	Het
Pkd1l3	C	T	8: 110,365,019 (GRCm39)	Q1124*	probably null	Het
Plcd3	A	G	11: 102,965,757 (GRCm39)	S492P	possibly damaging	Het
Rcor2	T	G	19: 7,248,411 (GRCm39)	M142R	probably benign	Het
Rwdd3	T	C	3: 120,950,101 (GRCm39)	E115G	probably damaging	Het
Slc13a3	T	C	2: 165,250,818 (GRCm39)	Y475C	probably damaging	Het
Slc14a2	A	T	18: 78,202,297 (GRCm39)	I611N	possibly damaging	Het
Slc25a24	G	T	3: 109,043,617 (GRCm39)	M91I	probably damaging	Het
Spg11	T	C	2: 121,922,786 (GRCm39)	S888G	probably benign	Het
Spta1	A	T	1: 174,014,714 (GRCm39)		probably null	Het
Stk35	C	A	2: 129,652,607 (GRCm39)	D369E	possibly damaging	Het
Tanc1	T	C	2: 59,637,986 (GRCm39)	S889P	probably damaging	Het
Tbc1d10b	T	C	7: 126,798,050 (GRCm39)	N697S	probably damaging	Het
Tcof1	A	T	18: 60,947,852 (GRCm39)		probably null	Het
Tg	T	A	15: 66,543,160 (GRCm39)	Y25N	probably damaging	Het
Thbs1	T	C	2: 117,949,598 (GRCm39)	C563R	probably damaging	Het
Tlr11	A	G	14: 50,600,512 (GRCm39)	S833G	possibly damaging	Het
Uggt2	T	A	14: 119,294,976 (GRCm39)	H550L	probably benign	Het
Utp20	A	G	10: 88,591,048 (GRCm39)		probably null	Het
Wbp1l	T	A	19: 46,642,758 (GRCm39)	L253Q	probably damaging	Het
Zfp433	T	A	10: 81,556,078 (GRCm39)	S161R	possibly damaging	Het
Zfp865	A	G	7: 5,032,782 (GRCm39)	T256A	probably damaging	Het
Zpr1	A	G	9: 46,186,009 (GRCm39)	D160G	probably benign	Het

Other mutations in Cdc14b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00778:Cdc14b	APN	13	64,363,470 (GRCm39)	missense	probably damaging	1.00
IGL00816:Cdc14b	APN	13	64,353,217 (GRCm39)	missense	probably benign	0.10
IGL02569:Cdc14b	APN	13	64,373,428 (GRCm39)	missense	probably benign	0.36
IGL02634:Cdc14b	APN	13	64,364,117 (GRCm39)	splice site	probably benign
IGL02897:Cdc14b	APN	13	64,395,067 (GRCm39)	missense	probably benign	0.00
R0390:Cdc14b	UTSW	13	64,358,006 (GRCm39)	unclassified	probably benign
R0542:Cdc14b	UTSW	13	64,391,497 (GRCm39)	missense	probably benign	0.01
R1022:Cdc14b	UTSW	13	64,363,490 (GRCm39)	missense	probably damaging	1.00
R1024:Cdc14b	UTSW	13	64,363,490 (GRCm39)	missense	probably damaging	1.00
R1676:Cdc14b	UTSW	13	64,373,416 (GRCm39)	missense	possibly damaging	0.93
R1945:Cdc14b	UTSW	13	64,367,704 (GRCm39)	missense	probably damaging	1.00
R1964:Cdc14b	UTSW	13	64,363,351 (GRCm39)	missense	probably damaging	1.00
R3162:Cdc14b	UTSW	13	64,394,422 (GRCm39)	splice site	probably benign
R4359:Cdc14b	UTSW	13	64,396,225 (GRCm39)	missense	probably benign	0.27
R4598:Cdc14b	UTSW	13	64,395,088 (GRCm39)	missense	probably benign
R4716:Cdc14b	UTSW	13	64,357,014 (GRCm39)	missense	probably damaging	1.00
R6196:Cdc14b	UTSW	13	64,353,338 (GRCm39)	intron	probably benign
R6219:Cdc14b	UTSW	13	64,353,338 (GRCm39)	intron	probably benign
R6361:Cdc14b	UTSW	13	64,364,023 (GRCm39)	splice site	probably null
R6565:Cdc14b	UTSW	13	64,373,444 (GRCm39)	missense	probably benign	0.01
R6692:Cdc14b	UTSW	13	64,363,377 (GRCm39)	missense	probably damaging	0.98
R7204:Cdc14b	UTSW	13	64,358,012 (GRCm39)	missense	possibly damaging	0.83
R7327:Cdc14b	UTSW	13	64,373,461 (GRCm39)	missense	probably damaging	1.00
R7464:Cdc14b	UTSW	13	64,344,489 (GRCm39)	nonsense	probably null
R7639:Cdc14b	UTSW	13	64,353,143 (GRCm39)	missense	possibly damaging	0.96
R7687:Cdc14b	UTSW	13	64,357,007 (GRCm39)	missense	probably benign	0.15
R7949:Cdc14b	UTSW	13	64,338,212 (GRCm39)	splice site	probably null
R8170:Cdc14b	UTSW	13	64,363,549 (GRCm39)	splice site	probably null
R9047:Cdc14b	UTSW	13	64,368,758 (GRCm39)	intron	probably benign
Z1176:Cdc14b	UTSW	13	64,422,483 (GRCm39)	missense	possibly damaging	0.66

Predicted Primers

PCR Primer
(F):5'- TAACATGCCTTCCTGCCAG -3'
(R):5'- TCTGGATCTGGAAAATGTGCG -3'

Sequencing Primer
(F):5'- TCCTGCCAGGGCTTCAC -3'
(R):5'- CTGCATTTCTGGGCACAAG -3'

Posted On

2018-05-21