Incidental Mutation 'R7047:Ptgfr'
ID 547361
Institutional Source Beutler Lab
Gene Symbol Ptgfr
Ensembl Gene ENSMUSG00000028036
Gene Name prostaglandin F receptor
Synonyms FP, PGF
MMRRC Submission 045145-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.091) question?
Stock # R7047 (G1)
Quality Score 225.009
Status Validated
Chromosome 3
Chromosomal Location 151504247-151543165 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 151541178 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Asparagine at position 110 (I110N)
Ref Sequence ENSEMBL: ENSMUSP00000101732 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000029670] [ENSMUST00000106126]
AlphaFold P43117
Predicted Effect possibly damaging
Transcript: ENSMUST00000029670
AA Change: I110N

PolyPhen 2 Score 0.744 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000029670
Gene: ENSMUSG00000028036
AA Change: I110N

Pfam:7tm_1 23 304 6.8e-18 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000106126
AA Change: I110N

PolyPhen 2 Score 0.744 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000101732
Gene: ENSMUSG00000028036
AA Change: I110N

Pfam:7tm_1 43 304 7.6e-26 PFAM
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.7%
  • 20x: 99.1%
Validation Efficiency 98% (53/54)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is member of the G-protein coupled receptor family. This protein is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in uterus. Knockout studies in mice suggest that the interaction of PGF2-alpha with this receptor may initiate parturition in ovarian luteal cells and thus induce luteolysis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Pregnant females homozygous for a targeted null mutation are unable to deliver their offspring due to lack of induction of the oxytocin receptor and fail to show the normal decline of serum progesterone levels preceding parturition. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 53 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
3425401B19Rik A T 14: 32,382,131 (GRCm39) I1278N possibly damaging Het
Adcy5 T A 16: 35,087,585 (GRCm39) L482Q probably damaging Het
Adora2a A G 10: 75,162,145 (GRCm39) I95V probably damaging Het
Anapc1 A C 2: 128,457,350 (GRCm39) C1887G probably damaging Het
Arfgef2 T A 2: 166,693,865 (GRCm39) probably null Het
Capn11 G T 17: 45,949,622 (GRCm39) S448* probably null Het
Capn7 C T 14: 31,058,642 (GRCm39) probably benign Het
Cep55 T A 19: 38,048,539 (GRCm39) V65D possibly damaging Het
Col5a1 A G 2: 27,818,096 (GRCm39) K107R unknown Het
Dbh T A 2: 27,055,622 (GRCm39) I32N possibly damaging Het
Disp1 C A 1: 182,869,030 (GRCm39) R1130L probably damaging Het
Dzip3 A T 16: 48,802,489 (GRCm39) N13K probably benign Het
Emilin1 A G 5: 31,074,422 (GRCm39) N221S probably benign Het
Ext2 A G 2: 93,570,002 (GRCm39) F470L probably damaging Het
Filip1 T A 9: 79,760,916 (GRCm39) Q206L probably damaging Het
Garin1b T A 6: 29,323,809 (GRCm39) L178H probably damaging Het
Gpr63 G T 4: 25,007,320 (GRCm39) A15S probably benign Het
Grhl3 T C 4: 135,276,551 (GRCm39) probably null Het
Hspa5 C T 2: 34,663,204 (GRCm39) P127L probably damaging Het
Ift172 G T 5: 31,433,238 (GRCm39) Y193* probably null Het
Ints1 A C 5: 139,744,226 (GRCm39) L1512* probably null Het
Kat2b A G 17: 53,970,597 (GRCm39) Q661R probably benign Het
Kat6a A G 8: 23,428,554 (GRCm39) N1303S possibly damaging Het
L3mbtl4 G T 17: 68,768,561 (GRCm39) R223L probably benign Het
Maml2 T C 9: 13,532,177 (GRCm39) probably benign Het
Mixl1 A G 1: 180,524,183 (GRCm39) probably null Het
Mocs1 T C 17: 49,759,887 (GRCm39) probably null Het
Ms4a15 T G 19: 10,962,236 (GRCm39) N36T probably damaging Het
Msr1 G A 8: 40,095,657 (GRCm39) T2I possibly damaging Het
Nlgn1 A C 3: 25,490,199 (GRCm39) Y509* probably null Het
Nsmce1 C A 7: 125,090,606 (GRCm39) G4C probably benign Het
Or4c113 A T 2: 88,885,490 (GRCm39) F93L probably damaging Het
Or51v15-ps1 A G 7: 103,278,918 (GRCm39) V83A probably damaging Het
Pan3 G A 5: 147,483,175 (GRCm39) G452D probably damaging Het
Pcyox1 G T 6: 86,365,891 (GRCm39) P441H probably damaging Het
Phf12 T C 11: 77,904,099 (GRCm39) V273A probably damaging Het
Plcd4 A G 1: 74,591,148 (GRCm39) E181G possibly damaging Het
Rab40c A C 17: 26,138,458 (GRCm39) V8G probably damaging Het
Rasal3 T A 17: 32,615,458 (GRCm39) Y460F probably damaging Het
Rhbdf2 A G 11: 116,494,477 (GRCm39) probably null Het
Ryr2 T A 13: 11,839,286 (GRCm39) N496Y possibly damaging Het
Sacs A T 14: 61,450,451 (GRCm39) I4166F probably damaging Het
Sh2d3c T C 2: 32,611,172 (GRCm39) M1T probably null Het
Smarca2 C T 19: 26,646,555 (GRCm39) S651L possibly damaging Het
Sned1 A T 1: 93,213,540 (GRCm39) R1204S possibly damaging Het
Spata31d1b T C 13: 59,860,249 (GRCm39) Y41H probably damaging Het
Stab2 C T 10: 86,694,016 (GRCm39) C2075Y probably damaging Het
Tcte1 A T 17: 45,844,294 (GRCm39) Q90L possibly damaging Het
Tll2 C T 19: 41,074,679 (GRCm39) G945S probably damaging Het
Tmbim7 A T 5: 3,720,112 (GRCm39) T116S probably benign Het
Tnfrsf19 A T 14: 61,242,667 (GRCm39) C72* probably null Het
Usp17le T A 7: 104,417,640 (GRCm39) T501S probably benign Het
Wnt5b T A 6: 119,425,217 (GRCm39) probably benign Het
Other mutations in Ptgfr
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01322:Ptgfr APN 3 151,541,323 (GRCm39) missense probably benign 0.43
IGL02085:Ptgfr APN 3 151,541,437 (GRCm39) missense probably benign 0.00
IGL02110:Ptgfr APN 3 151,541,097 (GRCm39) missense probably damaging 0.97
IGL02971:Ptgfr APN 3 151,540,963 (GRCm39) missense probably benign 0.00
IGL03263:Ptgfr APN 3 151,541,500 (GRCm39) missense probably benign 0.00
R0048:Ptgfr UTSW 3 151,540,728 (GRCm39) missense possibly damaging 0.51
R0048:Ptgfr UTSW 3 151,540,728 (GRCm39) missense possibly damaging 0.51
R0602:Ptgfr UTSW 3 151,540,839 (GRCm39) missense probably damaging 1.00
R0624:Ptgfr UTSW 3 151,540,839 (GRCm39) missense probably damaging 1.00
R0633:Ptgfr UTSW 3 151,507,400 (GRCm39) missense probably benign 0.00
R1614:Ptgfr UTSW 3 151,507,416 (GRCm39) missense probably benign 0.44
R1930:Ptgfr UTSW 3 151,540,831 (GRCm39) missense probably benign 0.16
R1931:Ptgfr UTSW 3 151,540,831 (GRCm39) missense probably benign 0.16
R1989:Ptgfr UTSW 3 151,540,976 (GRCm39) nonsense probably null
R4596:Ptgfr UTSW 3 151,507,430 (GRCm39) missense probably damaging 1.00
R5899:Ptgfr UTSW 3 151,540,738 (GRCm39) missense probably damaging 0.96
R6295:Ptgfr UTSW 3 151,540,926 (GRCm39) missense probably benign 0.00
R6907:Ptgfr UTSW 3 151,540,938 (GRCm39) missense possibly damaging 0.95
R7320:Ptgfr UTSW 3 151,541,034 (GRCm39) missense probably benign 0.22
R8205:Ptgfr UTSW 3 151,541,418 (GRCm39) missense probably benign 0.04
R8420:Ptgfr UTSW 3 151,541,053 (GRCm39) missense possibly damaging 0.49
R9049:Ptgfr UTSW 3 151,541,404 (GRCm39) missense probably benign 0.24
R9352:Ptgfr UTSW 3 151,541,160 (GRCm39) missense probably damaging 1.00
R9537:Ptgfr UTSW 3 151,541,445 (GRCm39) missense possibly damaging 0.91
Z1176:Ptgfr UTSW 3 151,541,278 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer

Sequencing Primer
Posted On 2019-05-13