Mutagenetix > Incidental Mutation

Incidental Mutation 'R8002:Vmn1r21'

616399

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r21

Ensembl Gene

ENSMUSG00000115343

Gene Name

vomeronasal 1 receptor 21

Synonyms

V1rc28

MMRRC Submission

046042-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.121) question?

Stock #

R8002 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

57820549-57821442 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 57821199 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 82 (I82L)

Ref Sequence

ENSEMBL: ENSMUSP00000145398 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000203310] [ENSMUST00000203488] [ENSMUST00000226191]

AlphaFold

Q8R2C6

Predicted Effect

probably benign
Transcript: ENSMUST00000203310
AA Change: I82L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000145398
Gene: ENSMUSG00000115343
AA Change: I82L

Domain	Start	End	E-Value	Type
Pfam:V1R	27	107	1.8e-14	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203488
AA Change: I82L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000144978
Gene: ENSMUSG00000115343
AA Change: I82L

Domain	Start	End	E-Value	Type
Pfam:V1R	28	105	2e-13	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000226191
AA Change: I82L

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acsm2	C	T	7: 119,172,480 (GRCm39)	R133C	possibly damaging	Het
Adgrf4	T	C	17: 42,978,683 (GRCm39)	E220G	probably benign	Het
Alx3	T	A	3: 107,508,055 (GRCm39)	L188*	probably null	Het
Arhgef2	A	T	3: 88,554,117 (GRCm39)	I969F	probably damaging	Het
Atf6	A	G	1: 170,646,823 (GRCm39)	V350A	probably benign	Het
Atp1a3	C	A	7: 24,700,096 (GRCm39)	G88V	probably damaging	Het
Brd8	T	C	18: 34,741,609 (GRCm39)	T360A	probably benign	Het
Casp1	C	A	9: 5,303,164 (GRCm39)	T206K	possibly damaging	Het
Ccdc150	A	G	1: 54,311,656 (GRCm39)	E214G	probably damaging	Het
Ccdc81	T	C	7: 89,525,343 (GRCm39)	E477G	probably benign	Het
Celsr2	G	T	3: 108,311,285 (GRCm39)	R1409S	probably damaging	Het
Chd6	T	C	2: 160,832,241 (GRCm39)	D977G	probably damaging	Het
Cpne3	A	T	4: 19,528,232 (GRCm39)	F342I	probably damaging	Het
Crot	A	C	5: 9,043,599 (GRCm39)	S8A	probably benign	Het
Cyp11b2	T	C	15: 74,727,881 (GRCm39)	H67R	probably damaging	Het
Dnah1	A	T	14: 31,020,679 (GRCm39)	L1230H	probably damaging	Het
Dqx1	G	A	6: 83,035,558 (GRCm39)	D24N	probably damaging	Het
Gabrg3	T	C	7: 56,384,716 (GRCm39)	T282A	possibly damaging	Het
Gm13199	A	G	2: 5,867,458 (GRCm39)	S13P	unknown	Het
Gnptab	A	T	10: 88,276,130 (GRCm39)	D1139V	probably benign	Het
Hdac1-ps	T	C	17: 78,799,716 (GRCm39)	S236P	probably damaging	Het
Jtb	T	C	3: 90,141,251 (GRCm39)	S76P	probably benign	Het
Klk1b27	T	A	7: 43,705,445 (GRCm39)	D172E	probably benign	Het
Lpcat4	G	A	2: 112,074,699 (GRCm39)	V307I	probably benign	Het
Ltn1	A	C	16: 87,212,835 (GRCm39)	S575R	probably benign	Het
Map3k13	A	G	16: 21,723,878 (GRCm39)	T287A	probably benign	Het
Marco	T	G	1: 120,422,509 (GRCm39)	I58L	probably benign	Het
Nadk	G	T	4: 155,661,655 (GRCm39)		probably null	Het
Or11h7	A	T	14: 50,891,314 (GRCm39)	I207F	probably damaging	Het
Otof	G	A	5: 30,537,954 (GRCm39)	T1215I	probably benign	Het
Pigw	A	T	11: 84,769,249 (GRCm39)	C27S	probably benign	Het
Pla2g10	A	T	16: 13,542,912 (GRCm39)	M125K	unknown	Het
Rfx4	A	C	10: 84,676,721 (GRCm39)	M204L	probably damaging	Het
Septin1	T	C	7: 126,815,074 (GRCm39)	D209G	probably damaging	Het
Slc1a7	G	A	4: 107,869,473 (GRCm39)	V513M	probably benign	Het
Slc26a4	T	C	12: 31,597,969 (GRCm39)	D159G	probably benign	Het
Sptbn4	C	A	7: 27,117,417 (GRCm39)	S444I	possibly damaging	Het
Srebf2	C	T	15: 82,062,966 (GRCm39)	R468C	probably damaging	Het
Stard6	T	A	18: 70,633,597 (GRCm39)	D201E	possibly damaging	Het
Tas2r114	T	C	6: 131,666,102 (GRCm39)	T309A	probably damaging	Het
Tdrd6	C	A	17: 43,940,710 (GRCm39)	A113S	probably damaging	Het
Tigd2	T	G	6: 59,187,494 (GRCm39)	N120K	probably damaging	Het
Tomm70a	A	G	16: 56,957,097 (GRCm39)	N224S	probably damaging	Het
Tspo	T	C	15: 83,455,640 (GRCm39)	V9A	probably benign	Het
Vmn2r103	T	A	17: 20,019,511 (GRCm39)	C532S	probably damaging	Het
Vmn2r76	T	C	7: 85,879,271 (GRCm39)	N343S	probably benign	Het
Wdr62	T	C	7: 29,951,785 (GRCm39)	K665E	probably damaging	Het
Xpc	T	A	6: 91,469,287 (GRCm39)	N820I	probably damaging	Het
Zfp418	A	G	7: 7,184,873 (GRCm39)	T279A	probably benign	Het

Other mutations in Vmn1r21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00329:Vmn1r21	APN	6	57,821,049 (GRCm39)	missense	probably benign	0.05
IGL01366:Vmn1r21	APN	6	57,820,799 (GRCm39)	missense	probably benign	0.01
IGL01660:Vmn1r21	APN	6	57,821,222 (GRCm39)	missense	probably damaging	0.99
IGL02864:Vmn1r21	APN	6	57,820,661 (GRCm39)	missense	probably benign	0.13
IGL02961:Vmn1r21	APN	6	57,820,974 (GRCm39)	missense	probably benign	0.01
IGL03170:Vmn1r21	APN	6	57,820,847 (GRCm39)	missense	probably damaging	0.99
PIT4453001:Vmn1r21	UTSW	6	57,821,307 (GRCm39)	missense	probably benign	0.04
R1800:Vmn1r21	UTSW	6	57,820,799 (GRCm39)	missense	probably benign	0.01
R1928:Vmn1r21	UTSW	6	57,821,077 (GRCm39)	nonsense	probably null
R3407:Vmn1r21	UTSW	6	57,820,877 (GRCm39)	missense	probably damaging	1.00
R5566:Vmn1r21	UTSW	6	57,821,079 (GRCm39)	missense	probably benign	0.06
R6012:Vmn1r21	UTSW	6	57,820,891 (GRCm39)	missense	probably damaging	1.00
R6293:Vmn1r21	UTSW	6	57,821,255 (GRCm39)	missense	probably benign	0.19
R6473:Vmn1r21	UTSW	6	57,820,583 (GRCm39)	missense	probably damaging	0.99
R7128:Vmn1r21	UTSW	6	57,820,936 (GRCm39)	missense	probably damaging	0.97
R7489:Vmn1r21	UTSW	6	57,820,877 (GRCm39)	missense	probably damaging	1.00
R7559:Vmn1r21	UTSW	6	57,821,227 (GRCm39)	missense	probably damaging	0.99
R8218:Vmn1r21	UTSW	6	57,820,910 (GRCm39)	missense	noncoding transcript
R8467:Vmn1r21	UTSW	6	57,821,441 (GRCm39)	start codon destroyed	probably null	1.00
R8922:Vmn1r21	UTSW	6	57,820,829 (GRCm39)	missense	probably damaging	1.00
R8930:Vmn1r21	UTSW	6	57,820,998 (GRCm39)	missense	probably benign	0.01
R8932:Vmn1r21	UTSW	6	57,820,998 (GRCm39)	missense	probably benign	0.01
R8961:Vmn1r21	UTSW	6	57,820,829 (GRCm39)	missense	probably damaging	1.00
R9154:Vmn1r21	UTSW	6	57,821,348 (GRCm39)	missense	probably benign	0.36
Z1176:Vmn1r21	UTSW	6	57,820,563 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- GACCTTCATCTGGTGAGTCTC -3'
(R):5'- CCAAGCTGGATTTGGAGTCC -3'

Sequencing Primer
(F):5'- GAGTCTCACTCATATTGGTAAAACC -3'
(R):5'- CCAAGCTGGATTTGGAGTCCTAGTC -3'

Posted On

2020-01-23