Incidental Mutation 'R8542:Olfm3'
ID 659409
Institutional Source Beutler Lab
Gene Symbol Olfm3
Ensembl Gene ENSMUSG00000027965
Gene Name olfactomedin 3
Synonyms B230206G02Rik, optimedin
MMRRC Submission 068507-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.103) question?
Stock # R8542 (G1)
Quality Score 225.009
Status Validated
Chromosome 3
Chromosomal Location 114697727-114919371 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) A to T at 114916196 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Glutamine to Leucine at position 376 (Q376L)
Ref Sequence ENSEMBL: ENSMUSP00000060985 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000051309] [ENSMUST00000081752] [ENSMUST00000149158]
AlphaFold P63056
Predicted Effect probably benign
Transcript: ENSMUST00000051309
AA Change: Q376L

PolyPhen 2 Score 0.103 (Sensitivity: 0.93; Specificity: 0.86)
SMART Domains Protein: ENSMUSP00000060985
Gene: ENSMUSG00000027965
AA Change: Q376L

DomainStartEndE-ValueType
signal peptide 1 16 N/A INTRINSIC
Pfam:Noelin-1 46 145 3.9e-52 PFAM
Blast:OLF 162 207 3e-8 BLAST
OLF 220 470 4.33e-114 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000081752
AA Change: Q356L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
SMART Domains Protein: ENSMUSP00000080448
Gene: ENSMUSG00000027965
AA Change: Q356L

DomainStartEndE-ValueType
signal peptide 1 24 N/A INTRINSIC
Pfam:Noelin-1 25 125 7.6e-54 PFAM
Blast:OLF 142 187 3e-8 BLAST
OLF 200 450 4.33e-114 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000149158
SMART Domains Protein: ENSMUSP00000121097
Gene: ENSMUSG00000027965

DomainStartEndE-ValueType
Pfam:Noelin-1 12 112 2.5e-51 PFAM
Blast:OLF 129 174 1e-8 BLAST
Blast:OLF 187 210 1e-7 BLAST
Coding Region Coverage
  • 1x: 100.0%
  • 3x: 100.0%
  • 10x: 99.8%
  • 20x: 99.3%
Validation Efficiency 100% (58/58)
Allele List at MGI
Other mutations in this stock
Total: 54 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
A2m A G 6: 121,634,369 (GRCm39) T634A probably benign Het
Abca12 C T 1: 71,349,047 (GRCm39) probably null Het
Acsl5 A G 19: 55,280,259 (GRCm39) T511A probably damaging Het
Alkbh1 A G 12: 87,478,275 (GRCm39) F212S probably damaging Het
Ankrd35 A G 3: 96,589,353 (GRCm39) I191V probably damaging Het
Apmap T C 2: 150,428,385 (GRCm39) Q254R probably benign Het
Atp10b A G 11: 43,121,208 (GRCm39) D957G probably benign Het
Bag6 G A 17: 35,363,334 (GRCm39) G725S probably damaging Het
Braf T C 6: 39,604,693 (GRCm39) H592R probably benign Het
Cilp A T 9: 65,185,405 (GRCm39) D500V probably damaging Het
Col27a1 T A 4: 63,239,662 (GRCm39) probably null Het
Defa3 T A 8: 21,778,179 (GRCm39) probably null Het
Dst C T 1: 34,231,688 (GRCm39) H3272Y possibly damaging Het
Egfl8 A G 17: 34,833,243 (GRCm39) C168R probably damaging Het
Eppk1 C T 15: 75,994,319 (GRCm39) R854Q probably benign Het
Gipc3 T A 10: 81,174,055 (GRCm39) M240L possibly damaging Het
Gm49368 T C 7: 127,679,433 (GRCm39) Y192H probably damaging Het
Ier5l A G 2: 30,362,948 (GRCm39) I359T possibly damaging Het
Impg1 C T 9: 80,312,080 (GRCm39) E168K probably damaging Het
Iqcn C A 8: 71,166,520 (GRCm39) probably null Het
Kcnq5 G A 1: 21,549,648 (GRCm39) R360C probably damaging Het
Kctd2 G T 11: 115,320,310 (GRCm39) probably benign Het
Kdm5b A T 1: 134,533,512 (GRCm39) M534L possibly damaging Het
Kif14 T A 1: 136,396,495 (GRCm39) V267D possibly damaging Het
Klra3 A T 6: 130,310,096 (GRCm39) probably null Het
Lmod1 T C 1: 135,292,221 (GRCm39) F359L possibly damaging Het
Mmp24 A G 2: 155,641,634 (GRCm39) E154G probably benign Het
Morc3 G T 16: 93,644,319 (GRCm39) probably null Het
Mtus2 A C 5: 148,240,408 (GRCm39) K85T probably damaging Het
Ncam1 G T 9: 49,419,898 (GRCm39) T806K probably damaging Het
Noc2l A G 4: 156,326,187 (GRCm39) T425A probably benign Het
Or52m2 A G 7: 102,263,872 (GRCm39) V108A probably benign Het
Pax5 A T 4: 44,570,071 (GRCm39) Y272N probably damaging Het
Pfkp G T 13: 6,631,557 (GRCm39) A166E possibly damaging Het
Pnpt1 C A 11: 29,082,773 (GRCm39) probably null Het
Prss40 A G 1: 34,596,967 (GRCm39) W76R probably damaging Het
Rbms1 A C 2: 60,612,265 (GRCm39) V165G probably damaging Het
Repin1 G T 6: 48,574,279 (GRCm39) E403* probably null Het
Rpl3l A G 17: 24,954,754 (GRCm39) D369G probably damaging Het
Sdr42e2 C A 7: 120,417,137 (GRCm39) P194Q probably damaging Het
Slc29a3 A G 10: 60,566,401 (GRCm39) S111P probably damaging Het
Sorbs1 G C 19: 40,365,244 (GRCm39) R180G probably benign Het
St6galnac6 T A 2: 32,509,513 (GRCm39) I305N probably damaging Het
Tanc2 A G 11: 105,807,834 (GRCm39) E1288G probably damaging Het
Tdrd6 T C 17: 43,935,783 (GRCm39) E1755G probably damaging Het
Tenm4 A T 7: 96,461,139 (GRCm39) D895V probably damaging Het
Tmem190 T A 7: 4,787,157 (GRCm39) C120* probably null Het
Tmem209 C A 6: 30,497,237 (GRCm39) V318F probably damaging Het
Traf3ip3 T A 1: 192,876,851 (GRCm39) I196F probably damaging Het
Trpc4ap G C 2: 155,534,132 (GRCm39) P32R unknown Het
Usp20 T G 2: 30,901,636 (GRCm39) V467G possibly damaging Het
Vav3 A G 3: 109,410,787 (GRCm39) K206R probably damaging Het
Zbtb17 CCCCCACCTCCACAGACCCCA CCCCCACCTCCACAGACCCCACCTCCACAGACCCCA 4: 141,194,139 (GRCm39) probably benign Het
Zfp629 C A 7: 127,210,364 (GRCm39) E482* probably null Het
Other mutations in Olfm3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01656:Olfm3 APN 3 114,916,282 (GRCm39) missense probably damaging 1.00
IGL01686:Olfm3 APN 3 114,916,497 (GRCm39) missense probably benign 0.00
IGL01732:Olfm3 APN 3 114,890,649 (GRCm39) missense possibly damaging 0.82
IGL02539:Olfm3 APN 3 114,895,579 (GRCm39) missense possibly damaging 0.93
IGL02580:Olfm3 APN 3 114,916,157 (GRCm39) missense probably damaging 1.00
IGL02999:Olfm3 APN 3 114,916,397 (GRCm39) missense probably damaging 1.00
IGL03142:Olfm3 APN 3 114,890,679 (GRCm39) missense probably damaging 0.97
IGL03239:Olfm3 APN 3 114,916,243 (GRCm39) missense probably damaging 0.99
R0361:Olfm3 UTSW 3 114,914,622 (GRCm39) missense probably damaging 1.00
R0373:Olfm3 UTSW 3 114,916,454 (GRCm39) missense probably damaging 0.99
R0505:Olfm3 UTSW 3 114,916,330 (GRCm39) missense possibly damaging 0.46
R0689:Olfm3 UTSW 3 114,916,194 (GRCm39) missense probably benign 0.28
R0973:Olfm3 UTSW 3 114,895,635 (GRCm39) missense probably benign 0.00
R0973:Olfm3 UTSW 3 114,895,635 (GRCm39) missense probably benign 0.00
R0974:Olfm3 UTSW 3 114,895,635 (GRCm39) missense probably benign 0.00
R1253:Olfm3 UTSW 3 114,916,418 (GRCm39) missense probably damaging 0.98
R1293:Olfm3 UTSW 3 114,895,579 (GRCm39) missense possibly damaging 0.93
R1952:Olfm3 UTSW 3 114,895,589 (GRCm39) missense probably null 1.00
R2255:Olfm3 UTSW 3 114,915,842 (GRCm39) splice site probably null
R2334:Olfm3 UTSW 3 114,895,608 (GRCm39) nonsense probably null
R2510:Olfm3 UTSW 3 114,915,959 (GRCm39) missense probably damaging 1.00
R4222:Olfm3 UTSW 3 114,883,820 (GRCm39) nonsense probably null
R4716:Olfm3 UTSW 3 114,874,755 (GRCm39) missense probably benign 0.00
R4912:Olfm3 UTSW 3 114,895,589 (GRCm39) missense probably damaging 1.00
R5084:Olfm3 UTSW 3 114,698,202 (GRCm39) critical splice donor site probably null
R5649:Olfm3 UTSW 3 114,890,573 (GRCm39) missense probably damaging 0.99
R5681:Olfm3 UTSW 3 114,915,924 (GRCm39) missense probably benign 0.28
R5861:Olfm3 UTSW 3 114,916,052 (GRCm39) missense probably damaging 1.00
R5924:Olfm3 UTSW 3 114,916,187 (GRCm39) missense probably benign 0.44
R5929:Olfm3 UTSW 3 114,895,529 (GRCm39) missense probably damaging 0.97
R5958:Olfm3 UTSW 3 114,915,955 (GRCm39) missense probably damaging 0.99
R6166:Olfm3 UTSW 3 114,916,074 (GRCm39) missense probably damaging 1.00
R6299:Olfm3 UTSW 3 114,914,632 (GRCm39) missense probably damaging 1.00
R6804:Olfm3 UTSW 3 114,916,328 (GRCm39) missense probably benign 0.10
R7032:Olfm3 UTSW 3 114,883,805 (GRCm39) missense probably damaging 1.00
R7565:Olfm3 UTSW 3 114,916,393 (GRCm39) missense probably damaging 0.98
R7600:Olfm3 UTSW 3 114,890,589 (GRCm39) missense possibly damaging 0.65
R7976:Olfm3 UTSW 3 114,874,794 (GRCm39) missense probably benign 0.00
R8070:Olfm3 UTSW 3 114,895,604 (GRCm39) missense probably damaging 0.96
R8334:Olfm3 UTSW 3 114,916,206 (GRCm39) missense probably damaging 0.96
R8527:Olfm3 UTSW 3 114,916,196 (GRCm39) missense probably benign 0.10
R8835:Olfm3 UTSW 3 114,916,061 (GRCm39) missense probably damaging 1.00
R9063:Olfm3 UTSW 3 114,914,582 (GRCm39) missense probably benign 0.36
R9594:Olfm3 UTSW 3 114,883,785 (GRCm39) missense probably damaging 1.00
R9690:Olfm3 UTSW 3 114,890,594 (GRCm39) nonsense probably null
R9690:Olfm3 UTSW 3 114,890,593 (GRCm39) missense probably benign 0.39
R9719:Olfm3 UTSW 3 114,916,091 (GRCm39) nonsense probably null
Z1088:Olfm3 UTSW 3 114,698,317 (GRCm39) start gained probably benign
Z1177:Olfm3 UTSW 3 114,874,750 (GRCm39) missense probably benign 0.01
Predicted Primers PCR Primer
(F):5'- TGACTTAGGGAGAGTGCTAGCC -3'
(R):5'- TGTTCCAGGCATAAAGAGCTC -3'

Sequencing Primer
(F):5'- GTGCTAGCCCAAAGAAGCCTG -3'
(R):5'- GTTCCAGGCATAAAGAGCTCTATCTC -3'
Posted On 2021-01-18