Mutagenetix > Incidental Mutation

Incidental Mutation 'R7976:Olfm3'

650886

Institutional Source

Beutler Lab

Gene Symbol

Olfm3

Ensembl Gene

ENSMUSG00000027965

Gene Name

olfactomedin 3

Synonyms

optimedin, B230206G02Rik

MMRRC Submission

Accession Numbers

Is this an essential gene?

Probably non essential (E-score: 0.163) question?

Stock #

R7976 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

114904078-115125722 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 115081145 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 30 (V30A)

Ref Sequence

ENSEMBL: ENSMUSP00000060985 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051309] [ENSMUST00000081752] [ENSMUST00000149158]

AlphaFold

P63056

Predicted Effect

probably benign
Transcript: ENSMUST00000051309
AA Change: V30A

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000060985
Gene: ENSMUSG00000027965
AA Change: V30A

Domain	Start	End	E-Value	Type
signal peptide	1	16	N/A	INTRINSIC
Pfam:Noelin-1	46	145	3.9e-52	PFAM
Blast:OLF	162	207	3e-8	BLAST
OLF	220	470	4.33e-114	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000081752

SMART Domains

Protein: ENSMUSP00000080448
Gene: ENSMUSG00000027965

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:Noelin-1	25	125	7.6e-54	PFAM
Blast:OLF	142	187	3e-8	BLAST
OLF	200	450	4.33e-114	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000149158

SMART Domains

Protein: ENSMUSP00000121097
Gene: ENSMUSG00000027965

Domain	Start	End	E-Value	Type
Pfam:Noelin-1	12	112	2.5e-51	PFAM
Blast:OLF	129	174	1e-8	BLAST
Blast:OLF	187	210	1e-7	BLAST

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 70 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adssl1	A	C	12: 112,636,397	I341L	probably benign	Het
Aldh1b1	T	A	4: 45,803,092	M210K	possibly damaging	Het
Ankrd17	G	A	5: 90,283,592	Q778*	probably null	Het
Bmper	T	C	9: 23,406,810	V575A	probably damaging	Het
Brinp2	A	G	1: 158,246,343	V736A	probably benign	Het
Ccdc81	A	T	7: 89,866,515	L652*	probably null	Het
Cdt1	C	T	8: 122,571,846	R437W	probably damaging	Het
Ckb	A	C	12: 111,671,032	L165R	possibly damaging	Het
Col25a1	G	T	3: 130,496,426	G255V	probably damaging	Het
Ddx17	A	G	15: 79,535,955		probably null	Het
Ddx58	T	C	4: 40,209,894	M725V	probably damaging	Het
Dennd4a	G	T	9: 64,852,512	G300W	possibly damaging	Het
Dlg4	T	A	11: 70,039,182	I316N	probably damaging	Het
Dlgap2	C	T	8: 14,743,410	P467L	probably benign	Het
Dnah9	T	C	11: 65,841,401	I4226M	possibly damaging	Het
Drc1	G	A	5: 30,364,485	A734T	probably benign	Het
Entpd1	A	G	19: 40,612,421	M1V	probably null	Het
Exph5	T	G	9: 53,376,635	I1672S	possibly damaging	Het
Fam149b	T	A	14: 20,377,784	D379E	probably damaging	Het
Fgfr2	T	C	7: 130,185,344	T461A	probably damaging	Het
Frem1	A	G	4: 83,001,709	V469A	probably damaging	Het
Frem3	A	T	8: 80,611,602	K175*	probably null	Het
Fsd2	C	T	7: 81,559,881	G71E	probably benign	Het
Gcat	T	C	15: 79,034,988	I116T	probably damaging	Het
Gigyf2	G	A	1: 87,403,736	S202N	unknown	Het
Glra3	T	G	8: 56,112,876		probably null	Het
Golga4	C	T	9: 118,536,768	T296I	possibly damaging	Het
Herc1	C	T	9: 66,434,270	T1816I	possibly damaging	Het
Igfbpl1	C	A	4: 45,826,786	R3L	unknown	Het
Ighv15-2	A	T	12: 114,564,850	S28T	probably benign	Het
Kat2b	T	A	17: 53,648,807	M427K	probably benign	Het
Kdm4c	A	G	4: 74,377,669	T882A	probably damaging	Het
Kif1a	G	T	1: 93,039,774	F1138L	probably damaging	Het
Klhl20	A	T	1: 161,106,737	S237R	probably benign	Het
Lamc1	A	T	1: 153,247,268	N725K	probably damaging	Het
Lmtk3	A	G	7: 45,795,466	D1191G	unknown	Het
Ltbp1	C	A	17: 75,363,363	N1466K	possibly damaging	Het
Ly75	T	C	2: 60,365,088	E242G	probably damaging	Het
Lyrm1	T	C	7: 119,916,226	V113A	probably benign	Het
Mfsd13a	G	T	19: 46,372,007	A333S	probably benign	Het
Mllt10	T	C	2: 18,162,403	S380P	possibly damaging	Het
Mrc2	A	G	11: 105,348,003	K1295E	possibly damaging	Het
Muc5ac	A	T	7: 141,809,791	I2280F	unknown	Het
Neurod2	A	C	11: 98,327,197	F380L	probably damaging	Het
Ntrk3	G	A	7: 78,356,206	A469V	probably damaging	Het
Nup205	T	C	6: 35,198,953	F584L	probably damaging	Het
Oas1d	A	T	5: 120,919,147	Y272F	probably damaging	Het
Olfr1054	A	G	2: 86,332,720	V212A	probably benign	Het
Olfr1215	T	A	2: 89,001,629	I220F	probably damaging	Het
Olfr1472	A	G	19: 13,454,199	I106T	probably benign	Het
Pcdhga12	A	G	18: 37,768,374	Y753C	probably damaging	Het
Phox2b	A	G	5: 67,096,171	V294A	unknown	Het
Prdm2	A	T	4: 143,133,242	C1159*	probably null	Het
Ptpn12	G	T	5: 21,002,633	S275*	probably null	Het
Rnf223	A	T	4: 156,132,319	E50D	probably damaging	Het
Rrp12	A	T	19: 41,891,109	Y169N	probably benign	Het
Sgip1	T	C	4: 102,900,539		probably null	Het
Shank2	T	C	7: 144,411,061	I802T	probably damaging	Het
Skint2	A	T	4: 112,624,132	N64I	probably damaging	Het
Smpd3	A	C	8: 106,255,622	C617G	probably benign	Het
Tmem106c	G	C	15: 97,968,104	G192R	probably damaging	Het
Tmem72	T	C	6: 116,696,839	H106R	probably damaging	Het
Upf2	G	C	2: 6,026,115	V789L	unknown	Het
Vash1	A	G	12: 86,679,984		probably benign	Het
Vmn1r201	T	A	13: 22,474,705	Y30N	probably benign	Het
Xpr1	A	G	1: 155,290,289	F571L	possibly damaging	Het
Yif1a	T	C	19: 5,089,787	S87P	probably damaging	Het
Zer1	T	C	2: 30,107,508	Y462C	probably damaging	Het
Zfp597	G	A	16: 3,866,511	P127L	possibly damaging	Het
Zxdc	T	C	6: 90,398,767	S742P	probably benign	Het

Other mutations in Olfm3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01656:Olfm3	APN	3	115122633	missense	probably damaging	1.00
IGL01686:Olfm3	APN	3	115122848	missense	probably benign	0.00
IGL01732:Olfm3	APN	3	115097000	missense	possibly damaging	0.82
IGL02539:Olfm3	APN	3	115101930	missense	possibly damaging	0.93
IGL02580:Olfm3	APN	3	115122508	missense	probably damaging	1.00
IGL02999:Olfm3	APN	3	115122748	missense	probably damaging	1.00
IGL03142:Olfm3	APN	3	115097030	missense	probably damaging	0.97
IGL03239:Olfm3	APN	3	115122594	missense	probably damaging	0.99
R0361:Olfm3	UTSW	3	115120973	missense	probably damaging	1.00
R0373:Olfm3	UTSW	3	115122805	missense	probably damaging	0.99
R0505:Olfm3	UTSW	3	115122681	missense	possibly damaging	0.46
R0689:Olfm3	UTSW	3	115122545	missense	probably benign	0.28
R0973:Olfm3	UTSW	3	115101986	missense	probably benign	0.00
R0973:Olfm3	UTSW	3	115101986	missense	probably benign	0.00
R0974:Olfm3	UTSW	3	115101986	missense	probably benign	0.00
R1253:Olfm3	UTSW	3	115122769	missense	probably damaging	0.98
R1293:Olfm3	UTSW	3	115101930	missense	possibly damaging	0.93
R1952:Olfm3	UTSW	3	115101940	missense	probably null	1.00
R2255:Olfm3	UTSW	3	115122193	splice site	probably null
R2334:Olfm3	UTSW	3	115101959	nonsense	probably null
R2510:Olfm3	UTSW	3	115122310	missense	probably damaging	1.00
R4222:Olfm3	UTSW	3	115090171	nonsense	probably null
R4716:Olfm3	UTSW	3	115081106	missense	probably benign	0.00
R4912:Olfm3	UTSW	3	115101940	missense	probably damaging	1.00
R5084:Olfm3	UTSW	3	114904553	critical splice donor site	probably null
R5649:Olfm3	UTSW	3	115096924	missense	probably damaging	0.99
R5681:Olfm3	UTSW	3	115122275	missense	probably benign	0.28
R5861:Olfm3	UTSW	3	115122403	missense	probably damaging	1.00
R5924:Olfm3	UTSW	3	115122538	missense	probably benign	0.44
R5929:Olfm3	UTSW	3	115101880	missense	probably damaging	0.97
R5958:Olfm3	UTSW	3	115122306	missense	probably damaging	0.99
R6166:Olfm3	UTSW	3	115122425	missense	probably damaging	1.00
R6299:Olfm3	UTSW	3	115120983	missense	probably damaging	1.00
R6804:Olfm3	UTSW	3	115122679	missense	probably benign	0.10
R7032:Olfm3	UTSW	3	115090156	missense	probably damaging	1.00
R7565:Olfm3	UTSW	3	115122744	missense	probably damaging	0.98
R7600:Olfm3	UTSW	3	115096940	missense	possibly damaging	0.65
R8070:Olfm3	UTSW	3	115101955	missense	probably damaging	0.96
R8334:Olfm3	UTSW	3	115122557	missense	probably damaging	0.96
R8527:Olfm3	UTSW	3	115122547	missense	probably benign	0.10
R8542:Olfm3	UTSW	3	115122547	missense	probably benign	0.10
R8835:Olfm3	UTSW	3	115122412	missense	probably damaging	1.00
R9063:Olfm3	UTSW	3	115120933	missense	probably benign	0.36
R9594:Olfm3	UTSW	3	115090136	missense	probably damaging	1.00
Z1088:Olfm3	UTSW	3	114904668	start gained	probably benign
Z1177:Olfm3	UTSW	3	115081101	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ATGTGGCTTGCTGCAGTCAC -3'
(R):5'- TTACAAAGGTTGCACGCATTG -3'

Sequencing Primer
(F):5'- TGCTGCAGTCACGCCTC -3'
(R):5'- GAGACCAACCTTCCTTTTTAGAGAC -3'

Posted On

2020-09-15