Mutagenetix > Incidental Mutation

Incidental Mutation 'R8872:Zbtb26'

676333

Institutional Source

Beutler Lab

Gene Symbol

Zbtb26

Ensembl Gene

ENSMUSG00000050714

Gene Name

zinc finger and BTB domain containing 26

Synonyms

A630026F21Rik

MMRRC Submission

068743-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.365) question?

Stock #

R8872 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

37322180-37333147 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 37326913 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Isoleucine at position 41 (N41I)

Ref Sequence

ENSEMBL: ENSMUSP00000099850 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000067043] [ENSMUST00000102789] [ENSMUST00000112932]

AlphaFold

Q8C8S0

Predicted Effect

probably damaging
Transcript: ENSMUST00000067043
AA Change: N30I

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000070071
Gene: ENSMUSG00000050714
AA Change: N30I

Domain	Start	End	E-Value	Type
BTB	33	127	4.38e-12	SMART
low complexity region	169	179	N/A	INTRINSIC
ZnF_C2H2	273	295	1.36e-2	SMART
ZnF_C2H2	298	320	1.4e-4	SMART
ZnF_C2H2	326	348	2.99e-4	SMART
ZnF_C2H2	354	377	2.09e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000102789
AA Change: N41I

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000099850
Gene: ENSMUSG00000050714
AA Change: N41I

Domain	Start	End	E-Value	Type
BTB	44	138	4.38e-12	SMART
low complexity region	180	190	N/A	INTRINSIC
ZnF_C2H2	284	306	1.36e-2	SMART
ZnF_C2H2	309	331	1.4e-4	SMART
ZnF_C2H2	337	359	2.99e-4	SMART
ZnF_C2H2	365	388	2.09e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112932

SMART Domains

Protein: ENSMUSP00000108554
Gene: ENSMUSG00000066798

Domain	Start	End	E-Value	Type
BTB	33	127	2.67e-16	SMART
Blast:BTB	161	196	1e-5	BLAST
ZnF_C2H2	300	322	7.15e-2	SMART
ZnF_C2H2	325	347	1.58e-3	SMART
ZnF_C2H2	353	375	3.63e-3	SMART
ZnF_C2H2	381	404	8.81e-2	SMART

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.8%
20x: 99.5%

Validation Efficiency

99% (74/75)

Allele List at MGI

Other mutations in this stock

Total: 73 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700067P10Rik	A	G	17: 48,401,164 (GRCm39)	S150G	possibly damaging	Het
Alpk2	A	T	18: 65,413,977 (GRCm39)	S1579R	probably damaging	Het
Ank2	C	T	3: 126,791,525 (GRCm39)	E814K	possibly damaging	Het
Cep57	A	G	9: 13,737,980 (GRCm39)		probably benign	Het
Cntnap4	A	T	8: 113,585,759 (GRCm39)	R1127S	possibly damaging	Het
Comt	C	A	16: 18,245,239 (GRCm39)		probably benign	Het
Crlf3	T	C	11: 79,938,440 (GRCm39)	N399S		Het
Crls1	T	A	2: 132,691,819 (GRCm39)	S115T	probably benign	Het
Dixdc1	A	G	9: 50,614,453 (GRCm39)	S199P	possibly damaging	Het
Dsc3	T	C	18: 20,122,679 (GRCm39)	T82A	probably benign	Het
Efcab3	T	G	11: 104,760,880 (GRCm39)	L2411R	probably benign	Het
Exosc6	T	A	8: 111,783,784 (GRCm39)	V261E	probably damaging	Het
Fbxw17	C	T	13: 50,586,300 (GRCm39)	S361L	probably benign	Het
Gfap	T	A	11: 102,786,620 (GRCm39)	N157Y	possibly damaging	Het
Gigyf2	A	T	1: 87,307,725 (GRCm39)	D177V	unknown	Het
Gls2	A	T	10: 128,040,535 (GRCm39)	Q312L	probably benign	Het
Gpr155	C	T	2: 73,197,936 (GRCm39)	V395I	probably benign	Het
Greb1l	A	T	18: 10,529,684 (GRCm39)	M889L	probably benign	Het
Gucy1a1	T	A	3: 82,016,049 (GRCm39)	D313V	probably damaging	Het
H2-T5	T	A	17: 36,476,293 (GRCm39)	I324F	probably benign	Het
Herpud1	C	T	8: 95,113,213 (GRCm39)		probably benign	Het
Idh2	TCCCAGG	T	7: 79,748,079 (GRCm39)		probably benign	Het
Il27	A	C	7: 126,190,194 (GRCm39)	L140R	probably damaging	Het
Jarid2	T	C	13: 45,055,984 (GRCm39)	S397P	possibly damaging	Het
Kdm5a	A	C	6: 120,365,101 (GRCm39)	D334A	probably damaging	Het
Lman2	T	C	13: 55,496,197 (GRCm39)	T283A	probably benign	Het
Lrfn2	C	T	17: 49,378,277 (GRCm39)	Q453*	probably null	Het
Lrrfip2	A	G	9: 111,034,824 (GRCm39)	E171G	possibly damaging	Het
Mboat1	T	A	13: 30,410,397 (GRCm39)	Y285N	probably damaging	Het
Med15	C	A	16: 17,470,605 (GRCm39)	S734I	probably damaging	Het
Mgat4c	T	A	10: 102,224,146 (GRCm39)	I120N	probably damaging	Het
Ms4a4d	T	C	19: 11,530,251 (GRCm39)	M104T	possibly damaging	Het
Mtf2	A	T	5: 108,247,051 (GRCm39)	M330L	probably benign	Het
Myoc	T	C	1: 162,475,013 (GRCm39)	V188A	probably benign	Het
Nos2	T	A	11: 78,839,949 (GRCm39)	I686N	probably damaging	Het
Npffr1	T	C	10: 61,461,794 (GRCm39)	V310A	probably benign	Het
Nrap	T	C	19: 56,308,627 (GRCm39)	*1729W	probably null	Het
Odam	A	G	5: 88,035,797 (GRCm39)		probably null	Het
Olfm4	G	T	14: 80,258,943 (GRCm39)	R397L	probably damaging	Het
Or1p1c	T	G	11: 74,160,120 (GRCm39)		probably benign	Het
Polr1b	T	C	2: 128,957,613 (GRCm39)	V556A	probably damaging	Het
Prl3d3	A	G	13: 27,346,324 (GRCm39)	D186G	possibly damaging	Het
Psmc5	C	A	11: 106,152,746 (GRCm39)	Y189*	probably null	Het
Rasgrp4	A	G	7: 28,838,521 (GRCm39)	Y123C	possibly damaging	Het
Rtn4	A	G	11: 29,658,633 (GRCm39)	E929G	probably benign	Het
Scrn2	A	T	11: 96,922,961 (GRCm39)	I135F	probably damaging	Het
Sec24d	T	A	3: 123,148,585 (GRCm39)		probably benign	Het
Secisbp2l	T	C	2: 125,594,892 (GRCm39)	T523A	probably benign	Het
Skic3	T	C	13: 76,333,326 (GRCm39)	L1525P	probably damaging	Het
Slain1	T	A	14: 103,925,841 (GRCm39)		probably null	Het
Slc22a29	A	G	19: 8,137,931 (GRCm39)	V548A	probably damaging	Het
Slc25a32	T	C	15: 38,969,339 (GRCm39)	I65V	probably benign	Het
Slc30a7	A	T	3: 115,740,317 (GRCm39)	M378K	possibly damaging	Het
Smarcd1	T	A	15: 99,608,975 (GRCm39)	I383N	probably damaging	Het
Spata31h1	A	T	10: 82,128,619 (GRCm39)	S1464T	probably benign	Het
Spdye4b	G	T	5: 143,187,815 (GRCm39)	K156N	probably damaging	Het
Sptb	A	G	12: 76,658,813 (GRCm39)	Y1241H	probably benign	Het
Ssu2	G	A	6: 112,357,956 (GRCm39)	T129I	probably damaging	Het
Supt16	A	G	14: 52,411,544 (GRCm39)	V613A	probably benign	Het
Sycp3	A	T	10: 88,302,388 (GRCm39)	E126V	probably damaging	Het
Tatdn2	A	T	6: 113,681,170 (GRCm39)	Y401F	probably damaging	Het
Tigd4	T	C	3: 84,501,547 (GRCm39)	S155P	probably benign	Het
Tnrc6b	A	G	15: 80,802,290 (GRCm39)	N1502S	probably benign	Het
Ttf2	A	G	3: 100,870,644 (GRCm39)	V143A	probably benign	Het
Ttn	T	A	2: 76,618,747 (GRCm39)	D16179V	probably damaging	Het
Vmn2r106	A	T	17: 20,488,401 (GRCm39)	M666K	probably benign	Het
Vwa5b1	A	T	4: 138,305,956 (GRCm39)	V914D	probably damaging	Het
Wnk2	T	C	13: 49,210,960 (GRCm39)	M1632V	probably benign	Het
Zc3h12a	T	C	4: 125,020,412 (GRCm39)	N144D	probably damaging	Het
Zfp266	A	T	9: 20,411,275 (GRCm39)	C301S	probably benign	Het
Zfp580	A	G	7: 5,056,216 (GRCm39)	H192R	possibly damaging	Het
Zfp748	A	G	13: 67,689,914 (GRCm39)	C449R	probably damaging	Het
Zp2	T	C	7: 119,733,025 (GRCm39)	I612V	probably benign	Het

Other mutations in Zbtb26

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00579:Zbtb26	APN	2	37,326,454 (GRCm39)	missense	possibly damaging	0.82
IGL00899:Zbtb26	APN	2	37,326,270 (GRCm39)	nonsense	probably null
IGL01598:Zbtb26	APN	2	37,326,283 (GRCm39)	missense	probably damaging	1.00
IGL01940:Zbtb26	APN	2	37,325,987 (GRCm39)	missense	possibly damaging	0.93
IGL02152:Zbtb26	APN	2	37,326,703 (GRCm39)	missense	possibly damaging	0.95
IGL02867:Zbtb26	APN	2	37,326,261 (GRCm39)	missense	probably benign	0.00
IGL02889:Zbtb26	APN	2	37,326,261 (GRCm39)	missense	probably benign	0.00
IGL03081:Zbtb26	APN	2	37,326,612 (GRCm39)	missense	possibly damaging	0.67
R0138:Zbtb26	UTSW	2	37,326,053 (GRCm39)	missense	probably benign	0.16
R0328:Zbtb26	UTSW	2	37,326,807 (GRCm39)	missense	possibly damaging	0.81
R0927:Zbtb26	UTSW	2	37,326,337 (GRCm39)	missense	possibly damaging	0.91
R1671:Zbtb26	UTSW	2	37,326,377 (GRCm39)	missense	probably benign	0.00
R1813:Zbtb26	UTSW	2	37,326,347 (GRCm39)	missense	possibly damaging	0.68
R1896:Zbtb26	UTSW	2	37,326,347 (GRCm39)	missense	possibly damaging	0.68
R2128:Zbtb26	UTSW	2	37,326,563 (GRCm39)	missense	probably benign	0.00
R2374:Zbtb26	UTSW	2	37,326,497 (GRCm39)	missense	probably benign
R4050:Zbtb26	UTSW	2	37,327,000 (GRCm39)	start codon destroyed	probably null	0.46
R4631:Zbtb26	UTSW	2	37,326,968 (GRCm39)	missense	probably benign	0.00
R4940:Zbtb26	UTSW	2	37,326,781 (GRCm39)	missense	probably damaging	0.98
R5071:Zbtb26	UTSW	2	37,325,941 (GRCm39)	missense	probably benign	0.26
R6348:Zbtb26	UTSW	2	37,325,687 (GRCm39)	missense	probably benign	0.43
R6962:Zbtb26	UTSW	2	37,326,106 (GRCm39)	missense	possibly damaging	0.71
R6990:Zbtb26	UTSW	2	37,326,557 (GRCm39)	missense	probably benign	0.00
R7261:Zbtb26	UTSW	2	37,326,667 (GRCm39)	missense	possibly damaging	0.71
R7981:Zbtb26	UTSW	2	37,326,887 (GRCm39)	missense	possibly damaging	0.51
R8013:Zbtb26	UTSW	2	37,327,013 (GRCm39)	critical splice acceptor site	probably null
R8014:Zbtb26	UTSW	2	37,327,013 (GRCm39)	critical splice acceptor site	probably null
R8876:Zbtb26	UTSW	2	37,326,896 (GRCm39)	missense	probably benign
R8905:Zbtb26	UTSW	2	37,326,927 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- AGTTCCATCTCAGGGAATTCCAG -3'
(R):5'- ACAAGCATGACAACACATTTGG -3'

Sequencing Primer
(F):5'- TCTCAGGGAATTCCAGCACAC -3'
(R):5'- GCATGACAACACATTTGGTTTTG -3'

Posted On

2021-07-15