Mutagenetix > Incidental Mutation

Incidental Mutation 'R9640:Zfp932'

725913

Institutional Source

Beutler Lab

Gene Symbol

Zfp932

Ensembl Gene

ENSMUSG00000066613

Gene Name

zinc finger protein 932

Synonyms

2310001H12Rik

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.078) question?

Stock #

R9640 (G1)

Quality Score

200.009

Status

Not validated

Chromosome

Chromosomal Location

110144387-110158277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 110157064 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 254 (V254A)

Ref Sequence

ENSEMBL: ENSMUSP00000108159 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099484] [ENSMUST00000112536] [ENSMUST00000112540] [ENSMUST00000125213] [ENSMUST00000143639] [ENSMUST00000187241]

AlphaFold

E9QAG8

Predicted Effect

probably benign
Transcript: ENSMUST00000099484

SMART Domains

Protein: ENSMUSP00000097083
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112536
AA Change: V221A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000108155
Gene: ENSMUSG00000066613
AA Change: V221A

Domain	Start	End	E-Value	Type
Blast:KRAB	1	31	5e-12	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
ZnF_C2H2	183	205	1.3e-4	SMART
ZnF_C2H2	211	233	1.1e-2	SMART
ZnF_C2H2	239	261	2.27e-4	SMART
ZnF_C2H2	267	289	1.84e-4	SMART
ZnF_C2H2	295	317	5.21e-4	SMART
ZnF_C2H2	323	345	9.73e-4	SMART
ZnF_C2H2	351	373	1.58e-3	SMART
ZnF_C2H2	379	401	1.58e-3	SMART
ZnF_C2H2	407	429	5.14e-3	SMART
ZnF_C2H2	435	457	2.61e-4	SMART
ZnF_C2H2	463	485	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112540
AA Change: V254A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000108159
Gene: ENSMUSG00000066613
AA Change: V254A

Domain	Start	End	E-Value	Type
KRAB	5	61	1.74e-14	SMART
ZnF_C2H2	104	126	1.1e-2	SMART
ZnF_C2H2	132	154	5.21e-4	SMART
ZnF_C2H2	160	182	3.39e-3	SMART
ZnF_C2H2	188	210	1.76e-1	SMART
ZnF_C2H2	216	238	1.3e-4	SMART
ZnF_C2H2	244	266	1.1e-2	SMART
ZnF_C2H2	272	294	2.27e-4	SMART
ZnF_C2H2	300	322	1.84e-4	SMART
ZnF_C2H2	328	350	5.21e-4	SMART
ZnF_C2H2	356	378	9.73e-4	SMART
ZnF_C2H2	384	406	1.58e-3	SMART
ZnF_C2H2	412	434	1.58e-3	SMART
ZnF_C2H2	440	462	5.14e-3	SMART
ZnF_C2H2	468	490	2.61e-4	SMART
ZnF_C2H2	496	518	2.43e-4	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000125213

SMART Domains

Protein: ENSMUSP00000119843
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	2e-14	BLAST
ZnF_C2H2	71	93	1.1e-2	SMART
ZnF_C2H2	99	121	5.21e-4	SMART
ZnF_C2H2	127	149	3.39e-3	SMART
ZnF_C2H2	155	177	1.76e-1	SMART
Pfam:zf-C2H2_6	182	191	7.3e-1	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000143639

SMART Domains

Protein: ENSMUSP00000121749
Gene: ENSMUSG00000066613

Domain	Start	End	E-Value	Type
KRAB	4	60	1.74e-14	SMART
ZnF_C2H2	103	125	1.1e-2	SMART
ZnF_C2H2	131	153	5.21e-4	SMART
ZnF_C2H2	159	181	3.39e-3	SMART
ZnF_C2H2	187	209	1.76e-1	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000187241
AA Change: V254A

PolyPhen 2 Score 0.005 (Sensitivity: 0.97; Specificity: 0.74)

SMART Domains

Protein: ENSMUSP00000140446
Gene: ENSMUSG00000066613
AA Change: V254A

Domain	Start	End	E-Value	Type
KRAB	5	61	7.1e-17	SMART
ZnF_C2H2	104	126	4.8e-5	SMART
ZnF_C2H2	132	154	2.2e-6	SMART
ZnF_C2H2	160	182	1.5e-5	SMART
ZnF_C2H2	188	210	7.2e-4	SMART
ZnF_C2H2	216	238	5.4e-7	SMART
ZnF_C2H2	244	266	4.5e-5	SMART
ZnF_C2H2	272	294	9.2e-7	SMART
ZnF_C2H2	300	322	7.5e-7	SMART
ZnF_C2H2	328	350	2.1e-6	SMART
ZnF_C2H2	356	378	4e-6	SMART
ZnF_C2H2	384	406	6.5e-6	SMART
ZnF_C2H2	412	434	6.9e-6	SMART
ZnF_C2H2	440	462	2.1e-5	SMART
ZnF_C2H2	468	490	1.1e-6	SMART
ZnF_C2H2	496	518	1.1e-6	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 59 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A630010A05Rik	T	C	16: 14,436,589 (GRCm39)	V214A		Het
A830018L16Rik	A	G	1: 12,021,200 (GRCm39)	K403R	probably damaging	Het
Aadacl4fm4	T	A	4: 144,412,809 (GRCm39)	R44W	possibly damaging	Het
Abhd17c	A	T	7: 83,800,814 (GRCm39)	L80Q	probably damaging	Het
Adam30	T	A	3: 98,069,620 (GRCm39)	D484E	probably damaging	Het
Aspm	T	A	1: 139,408,010 (GRCm39)	V2299E	possibly damaging	Het
Atp6v1c1	C	T	15: 38,689,381 (GRCm39)	R281W	probably damaging	Het
Bclaf1	T	C	10: 20,201,553 (GRCm39)		probably null	Het
Ccdc50	T	C	16: 27,225,461 (GRCm39)	V58A	probably damaging	Het
Cep162	A	G	9: 87,126,352 (GRCm39)	S187P	probably benign	Het
Clip4	C	T	17: 72,163,264 (GRCm39)	T612M	possibly damaging	Het
Corin	C	T	5: 72,592,597 (GRCm39)	V267I	probably benign	Het
Cyp2c37	T	A	19: 40,000,180 (GRCm39)	I434K	probably benign	Het
Ddx43	T	G	9: 78,303,389 (GRCm39)	D76E	probably benign	Het
Dusp5	A	G	19: 53,526,051 (GRCm39)	E231G	probably damaging	Het
Fam114a1	G	A	5: 65,166,394 (GRCm39)	V242I	probably damaging	Het
Frem1	T	A	4: 82,831,896 (GRCm39)	R1973S	probably benign	Het
Gas2l3	A	T	10: 89,266,774 (GRCm39)	D55E	probably damaging	Het
Grwd1	T	C	7: 45,477,303 (GRCm39)	I195V	probably benign	Het
H2-M1	G	T	17: 36,983,031 (GRCm39)	T20K	probably benign	Het
H2-Q2	T	G	17: 35,562,206 (GRCm39)	L151R	probably damaging	Het
Hbegf	T	C	18: 36,640,643 (GRCm39)	Y138C	probably damaging	Het
Hectd1	C	A	12: 51,795,197 (GRCm39)	E2411*	probably null	Het
Igkv11-125	T	A	6: 67,890,783 (GRCm39)	S34T	possibly damaging	Het
Jam2	A	T	16: 84,609,960 (GRCm39)	T191S	probably benign	Het
Kcnh8	T	C	17: 53,185,089 (GRCm39)	I457T	probably damaging	Het
Khk	A	G	5: 31,079,303 (GRCm39)	D15G	probably damaging	Het
Lrba	T	A	3: 86,526,875 (GRCm39)	Y2244*	probably null	Het
Lrp1b	T	C	2: 41,078,929 (GRCm39)	N1841S		Het
Lrp4	A	G	2: 91,316,296 (GRCm39)	T805A	probably benign	Het
Lrp6	C	T	6: 134,441,414 (GRCm39)	R1184Q	probably damaging	Het
Map3k1	A	T	13: 111,900,699 (GRCm39)	L442*	probably null	Het
Mapk8ip3	T	C	17: 25,155,647 (GRCm39)	N61S	possibly damaging	Het
Matcap2	A	T	9: 22,351,228 (GRCm39)	I457L	possibly damaging	Het
Mdn1	T	A	4: 32,754,539 (GRCm39)	V4685E	probably damaging	Het
Mib2	T	C	4: 155,745,325 (GRCm39)	E134G	possibly damaging	Het
Mtss2	T	C	8: 111,464,575 (GRCm39)	V403A	probably benign	Het
Ndst4	C	A	3: 125,232,196 (GRCm39)	T255K	probably damaging	Het
Or1i2	G	C	10: 78,448,311 (GRCm39)	L55V	probably damaging	Het
Or2l13	A	T	16: 19,305,761 (GRCm39)	M58L	probably damaging	Het
Or5ae2	G	A	7: 84,506,114 (GRCm39)	C181Y	probably damaging	Het
Or8g20	A	G	9: 39,396,396 (GRCm39)	L51P	probably damaging	Het
Pak1	T	A	7: 97,515,355 (GRCm39)	D126E	probably benign	Het
Rgl1	T	C	1: 152,397,142 (GRCm39)	T675A	probably damaging	Het
Rgs1	T	C	1: 144,121,116 (GRCm39)	D185G	probably damaging	Het
Rnase2a	G	A	14: 51,493,117 (GRCm39)	R83C	probably damaging	Het
Sfmbt2	A	G	2: 10,583,994 (GRCm39)	T784A	probably damaging	Het
Slc6a21	A	T	7: 44,937,189 (GRCm39)	T272S		Het
Sorcs2	G	T	5: 36,222,765 (GRCm39)	S330*	probably null	Het
Spdef	G	T	17: 27,936,183 (GRCm39)	H253Q		Het
Tas2r144	A	G	6: 42,192,428 (GRCm39)	E56G	probably benign	Het
Trim66	A	G	7: 109,074,825 (GRCm39)	C479R	probably damaging	Het
Ttc36	A	T	9: 44,714,034 (GRCm39)	L28Q	probably benign	Het
Ugt1a5	T	C	1: 88,094,098 (GRCm39)	S109P	possibly damaging	Het
Vamp5	G	A	6: 72,347,276 (GRCm39)		probably benign	Het
Wwox	A	G	8: 115,166,540 (GRCm39)	D10G	possibly damaging	Het
Zfp40	T	C	17: 23,394,493 (GRCm39)	Y698C	probably damaging	Het
Zfp62	A	G	11: 49,106,458 (GRCm39)	D183G	probably benign	Het
Zfp943	T	A	17: 22,209,899 (GRCm39)	L57H	probably damaging	Het

Other mutations in Zfp932

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0153:Zfp932	UTSW	5	110,154,834 (GRCm39)	missense	probably benign	0.27
R0268:Zfp932	UTSW	5	110,156,929 (GRCm39)	missense	probably benign	0.24
R1673:Zfp932	UTSW	5	110,156,854 (GRCm39)	missense	probably damaging	1.00
R1797:Zfp932	UTSW	5	110,144,489 (GRCm39)	start gained	probably benign
R1893:Zfp932	UTSW	5	110,157,069 (GRCm39)	missense	possibly damaging	0.71
R1942:Zfp932	UTSW	5	110,154,853 (GRCm39)	missense	probably damaging	1.00
R2073:Zfp932	UTSW	5	110,157,684 (GRCm39)	missense	possibly damaging	0.60
R2076:Zfp932	UTSW	5	110,157,334 (GRCm39)	missense	probably benign	0.01
R2329:Zfp932	UTSW	5	110,157,406 (GRCm39)	missense	probably benign	0.01
R3944:Zfp932	UTSW	5	110,157,820 (GRCm39)	missense	probably benign	0.37
R4551:Zfp932	UTSW	5	110,157,505 (GRCm39)	missense	probably benign	0.03
R4679:Zfp932	UTSW	5	110,157,760 (GRCm39)	missense	probably damaging	1.00
R4692:Zfp932	UTSW	5	110,157,052 (GRCm39)	missense	probably damaging	1.00
R5116:Zfp932	UTSW	5	110,157,242 (GRCm39)	missense	probably benign	0.05
R5260:Zfp932	UTSW	5	110,157,501 (GRCm39)	nonsense	probably null
R5536:Zfp932	UTSW	5	110,157,713 (GRCm39)	nonsense	probably null
R6351:Zfp932	UTSW	5	110,157,209 (GRCm39)	nonsense	probably null
R6786:Zfp932	UTSW	5	110,157,606 (GRCm39)	missense	probably damaging	0.98
R6843:Zfp932	UTSW	5	110,156,581 (GRCm39)	missense	probably benign	0.02
R7437:Zfp932	UTSW	5	110,157,880 (GRCm39)	missense	probably benign	0.10
R7496:Zfp932	UTSW	5	110,156,694 (GRCm39)	missense	probably damaging	0.97
R7704:Zfp932	UTSW	5	110,157,630 (GRCm39)	missense	probably benign	0.09
R8224:Zfp932	UTSW	5	110,144,480 (GRCm39)	start gained	probably benign
R8317:Zfp932	UTSW	5	110,156,922 (GRCm39)	nonsense	probably null
R8765:Zfp932	UTSW	5	110,154,827 (GRCm39)	missense	probably benign	0.01
R8967:Zfp932	UTSW	5	110,156,883 (GRCm39)	missense	probably benign	0.00
R9083:Zfp932	UTSW	5	110,157,100 (GRCm39)	missense	probably damaging	1.00
R9142:Zfp932	UTSW	5	110,157,520 (GRCm39)	missense	probably damaging	1.00
R9511:Zfp932	UTSW	5	110,155,177 (GRCm39)	missense	possibly damaging	0.46
R9533:Zfp932	UTSW	5	110,157,787 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGAATGTAATCAGTGTAGTAAAGCCT -3'
(R):5'- TGATCACACTTGTAAGGCTTCTC -3'

Sequencing Primer
(F):5'- CAGTGTAGTAAAGCCTTTGCAC -3'
(R):5'- GTAAGGCTTCTCTCCAGTATGAATTC -3'

Posted On

2022-09-12