Mutagenetix > Incidental Mutation

Incidental Mutation 'R1571:Wdcp'

186221

Institutional Source

Beutler Lab

Gene Symbol

Wdcp

Ensembl Gene

ENSMUSG00000051721

Gene Name

WD repeat and coiled coil containing

Synonyms

BC068281

MMRRC Submission

039610-MU

Accession Numbers

Essential gene?

Possibly essential (E-score: 0.707) question?

Stock #

R1571 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

4893303-4910043 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 4901924 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 593 (Y593*)

Ref Sequence

ENSEMBL: ENSMUSP00000152571 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053034] [ENSMUST00000085793] [ENSMUST00000220170] [ENSMUST00000220215] [ENSMUST00000223551]

AlphaFold

Q6NV72

Predicted Effect

probably null
Transcript: ENSMUST00000053034
AA Change: Y593*

SMART Domains

Protein: ENSMUSP00000054102
Gene: ENSMUSG00000051721
AA Change: Y593*

Domain	Start	End	E-Value	Type
Pfam:DUF4613	2	610	1.9e-260	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000085793
AA Change: Y593*

SMART Domains

Protein: ENSMUSP00000082948
Gene: ENSMUSG00000051721
AA Change: Y593*

Domain	Start	End	E-Value	Type
Pfam:DUF4613	2	643	2.4e-280	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219069

Predicted Effect

probably benign
Transcript: ENSMUST00000220170

Predicted Effect

probably null
Transcript: ENSMUST00000220215
AA Change: Y593*

Predicted Effect

probably null
Transcript: ENSMUST00000223551
AA Change: Y593*

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 96.4%
20x: 92.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 54 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610524H06Rik	A	G	5: 114,961,372 (GRCm39)		probably null	Het
Abcd3	T	C	3: 121,586,491 (GRCm39)	I70V	possibly damaging	Het
Acad10	T	C	5: 121,759,411 (GRCm39)	Y1024C	probably damaging	Het
Atp2b3	T	G	X: 72,588,712 (GRCm39)	V701G	probably damaging	Het
Cbfa2t2	T	A	2: 154,342,347 (GRCm39)	M21K	probably damaging	Het
Cdc25a	C	T	9: 109,710,614 (GRCm39)	T106I	possibly damaging	Het
Cdhr5	T	C	7: 140,852,083 (GRCm39)	T190A	probably damaging	Het
Chl1	A	G	6: 103,685,445 (GRCm39)	T829A	probably benign	Het
Clcn6	T	C	4: 148,097,226 (GRCm39)	T614A	possibly damaging	Het
Cntln	A	T	4: 84,865,823 (GRCm39)	R160*	probably null	Het
Dclk2	C	T	3: 86,712,946 (GRCm39)	R503Q	possibly damaging	Het
Dock3	C	A	9: 106,815,158 (GRCm39)	M1236I	possibly damaging	Het
Eif4g3	T	A	4: 137,847,719 (GRCm39)	H213Q	probably damaging	Het
Eya1	T	A	1: 14,279,141 (GRCm39)	H372L	probably damaging	Het
Fam13b	A	G	18: 34,630,485 (GRCm39)	V91A	possibly damaging	Het
Hat1	T	A	2: 71,264,519 (GRCm39)	I319K	probably benign	Het
Kcnf1	T	C	12: 17,225,853 (GRCm39)	N123D	probably benign	Het
Kcns3	C	A	12: 11,141,551 (GRCm39)	G383W	probably damaging	Het
Kprp	A	T	3: 92,732,689 (GRCm39)	C120*	probably null	Het
Lama3	T	A	18: 12,672,774 (GRCm39)	C2456S	probably damaging	Het
Lrp1b	A	T	2: 41,366,658 (GRCm39)	D539E	probably damaging	Het
Matn3	T	C	12: 9,005,466 (GRCm39)	L292S	probably damaging	Het
Mbd3l1	T	A	9: 18,395,947 (GRCm39)	I24N	probably damaging	Het
Med10	T	C	13: 69,958,159 (GRCm39)	L37P	probably damaging	Het
Mrc1	A	T	2: 14,313,544 (GRCm39)	H925L	probably damaging	Het
Myo15a	T	A	11: 60,409,290 (GRCm39)	I3219N	probably damaging	Het
Nom1	C	T	5: 29,647,633 (GRCm39)	Q623*	probably null	Het
Nrm	T	A	17: 36,175,079 (GRCm39)	W136R	probably damaging	Het
Or8h8	A	C	2: 86,753,789 (GRCm39)	V29G	probably benign	Het
Pde7b	T	C	10: 20,288,836 (GRCm39)	N298S	probably benign	Het
Piezo2	C	T	18: 63,277,990 (GRCm39)	A305T	possibly damaging	Het
Pimreg	T	C	11: 71,936,042 (GRCm39)	L175P	possibly damaging	Het
Pkhd1l1	A	G	15: 44,390,237 (GRCm39)	D1451G	probably benign	Het
Ptpro	A	G	6: 137,355,128 (GRCm39)	S212G	probably benign	Het
Rhpn1	C	T	15: 75,585,967 (GRCm39)	R627C	possibly damaging	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Rnase4	T	G	14: 51,342,497 (GRCm39)	F74V	probably damaging	Het
Sbno2	A	G	10: 79,896,226 (GRCm39)		probably null	Het
Selp	T	C	1: 163,954,176 (GRCm39)	Y159H	probably damaging	Het
Senp6	A	G	9: 80,000,853 (GRCm39)	T21A	probably damaging	Het
Slco1a7	A	T	6: 141,700,135 (GRCm39)	C132*	probably null	Het
Slco3a1	T	C	7: 74,154,128 (GRCm39)	D148G	possibly damaging	Het
Smtn	G	A	11: 3,480,102 (GRCm39)	P373L	probably benign	Het
Snx20	A	T	8: 89,356,597 (GRCm39)	L73Q	probably damaging	Het
Sobp	A	G	10: 43,033,942 (GRCm39)	V128A	possibly damaging	Het
Tcerg1	A	G	18: 42,657,357 (GRCm39)	T280A	unknown	Het
Tgfbrap1	A	G	1: 43,088,973 (GRCm39)	V810A	probably benign	Het
Thbs1	C	A	2: 117,949,678 (GRCm39)	D589E	probably damaging	Het
Tjp2	A	G	19: 24,078,239 (GRCm39)	Y885H	probably damaging	Het
Tmem109	A	G	19: 10,849,993 (GRCm39)	S100P	probably damaging	Het
Trim36	T	C	18: 46,305,562 (GRCm39)	K462E	probably benign	Het
Vmn1r226	A	T	17: 20,908,538 (GRCm39)	I257F	probably damaging	Het
Vmn2r92	A	G	17: 18,372,352 (GRCm39)	Y54C	probably damaging	Het
Wdr59	T	C	8: 112,177,682 (GRCm39)	S907G	probably damaging	Het

Other mutations in Wdcp

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00770:Wdcp	APN	12	4,905,303 (GRCm39)	missense	probably damaging	1.00
IGL00774:Wdcp	APN	12	4,905,303 (GRCm39)	missense	probably damaging	1.00
IGL02327:Wdcp	APN	12	4,901,115 (GRCm39)	missense	possibly damaging	0.95
IGL02725:Wdcp	APN	12	4,901,206 (GRCm39)	missense	probably damaging	1.00
IGL03377:Wdcp	APN	12	4,900,691 (GRCm39)	nonsense	probably null
IGL03381:Wdcp	APN	12	4,901,926 (GRCm39)	missense	probably damaging	1.00
R0152:Wdcp	UTSW	12	4,901,583 (GRCm39)	missense	probably benign	0.06
R0317:Wdcp	UTSW	12	4,901,583 (GRCm39)	missense	probably benign	0.06
R1077:Wdcp	UTSW	12	4,900,685 (GRCm39)	missense	probably damaging	1.00
R1136:Wdcp	UTSW	12	4,901,655 (GRCm39)	missense	possibly damaging	0.78
R1653:Wdcp	UTSW	12	4,901,815 (GRCm39)	missense	probably damaging	1.00
R1848:Wdcp	UTSW	12	4,900,245 (GRCm39)	missense	possibly damaging	0.86
R4091:Wdcp	UTSW	12	4,905,279 (GRCm39)	missense	probably null	0.00
R5126:Wdcp	UTSW	12	4,900,617 (GRCm39)	missense	probably damaging	0.98
R6148:Wdcp	UTSW	12	4,900,621 (GRCm39)	missense	possibly damaging	0.74
R6529:Wdcp	UTSW	12	4,901,143 (GRCm39)	missense	probably damaging	1.00
R6800:Wdcp	UTSW	12	4,901,358 (GRCm39)	missense	probably damaging	1.00
R7432:Wdcp	UTSW	12	4,900,246 (GRCm39)	missense	probably damaging	1.00
R7821:Wdcp	UTSW	12	4,907,975 (GRCm39)	missense	probably benign	0.07
R8182:Wdcp	UTSW	12	4,901,850 (GRCm39)	missense	probably damaging	1.00
R8553:Wdcp	UTSW	12	4,902,024 (GRCm39)	intron	probably benign
R8845:Wdcp	UTSW	12	4,901,439 (GRCm39)	missense	probably benign	0.26
R9134:Wdcp	UTSW	12	4,901,533 (GRCm39)	nonsense	probably null
R9609:Wdcp	UTSW	12	4,900,258 (GRCm39)	missense	probably damaging	0.98
U24488:Wdcp	UTSW	12	4,900,405 (GRCm39)	missense	probably damaging	1.00
X0026:Wdcp	UTSW	12	4,901,077 (GRCm39)	nonsense	probably null
Z1088:Wdcp	UTSW	12	4,900,825 (GRCm39)	missense	probably damaging	1.00
Z1176:Wdcp	UTSW	12	4,901,785 (GRCm39)	missense	possibly damaging	0.95

Predicted Primers

PCR Primer
(F):5'- TTTGCCCAGCATTGCCTAGACC -3'
(R):5'- TGTGCAAAGGAATGCCAAACTGCTC -3'

Sequencing Primer
(F):5'- ATTGCCTAGACCCAGCAGG -3'
(R):5'- ACTGCTCTAACAGGTCACTGG -3'

Posted On

2014-05-09