Mutagenetix > Incidental Mutation

Incidental Mutation 'R2304:Gm5449'

244537

Institutional Source

Beutler Lab

Gene Symbol

Gm5449

Ensembl Gene

ENSMUSG00000063166

Gene Name

predicted pseudogene 5449

Synonyms

MMRRC Submission

040303-MU

Accession Numbers

Essential gene?

Not available question?

Stock #

R2304 (G1)

Quality Score

Status

Not validated

Chromosome

Chromosomal Location

53679739-53680095 bp(+) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

G to T at 53679899 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000081132

SMART Domains

Protein: ENSMUSP00000079907
Gene: ENSMUSG00000063166

Domain	Start	End	E-Value	Type
Sm	32	111	1e-11	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000223156

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.4%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 23 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam22	T	A	5: 8,142,366 (GRCm39)	R806S	probably damaging	Het
Ccdc187	T	C	2: 26,171,029 (GRCm39)	K483R	possibly damaging	Het
Dcdc5	T	A	2: 106,166,488 (GRCm39)		noncoding transcript	Het
Dvl1	C	T	4: 155,940,041 (GRCm39)	S391F	probably damaging	Het
Erg28	A	G	12: 85,862,937 (GRCm39)	L125P	probably damaging	Het
Gkn3	C	T	6: 87,360,507 (GRCm39)	A163T	probably damaging	Het
Grid2ip	T	C	5: 143,373,595 (GRCm39)	Y796H	probably damaging	Het
Mcrip1	A	T	11: 120,435,519 (GRCm39)	F39I	probably damaging	Het
Or5w12	T	A	2: 87,502,238 (GRCm39)	I158L	probably benign	Het
Prss53	T	C	7: 127,487,479 (GRCm39)	N291D	probably benign	Het
Ptpru	A	T	4: 131,499,879 (GRCm39)	V1255D	probably damaging	Het
Rbl1	T	C	2: 156,989,551 (GRCm39)	T1023A	probably benign	Het
Rnaseh2b	T	C	14: 62,598,838 (GRCm39)	S188P	probably damaging	Het
Sh2d6	T	C	6: 72,497,542 (GRCm39)	E20G	probably damaging	Het
Slc13a5	A	G	11: 72,149,865 (GRCm39)	V172A	probably damaging	Het
Slco5a1	C	T	1: 12,949,486 (GRCm39)	G635S	probably damaging	Het
Sp8	G	T	12: 118,812,304 (GRCm39)	S53I	possibly damaging	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Togaram1	G	T	12: 65,023,630 (GRCm39)		probably null	Het
Trip11	A	T	12: 101,865,236 (GRCm39)	F146I	possibly damaging	Het
Vmn1r184	T	C	7: 25,966,550 (GRCm39)	S99P	probably damaging	Het
Xpot	T	C	10: 121,447,488 (GRCm39)	I325V	probably benign	Het
Zfp786	A	G	6: 47,797,633 (GRCm39)	L435P	probably damaging	Het

Other mutations in Gm5449

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R5302:Gm5449	UTSW	13	53,679,787 (GRCm39)	intron	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- AATCCTAGGGACTATGCAAGATTG -3'
(R):5'- AGCACCACGATGAAGGAGTC -3'

Sequencing Primer
(F):5'- GTGATCTGCGAGCGAAACCTAC -3'
(R):5'- GAGTCCCCATGCAGGAACATCTTAG -3'

Posted On

2014-10-30