Incidental Mutation 'R3924:Fmo9'
| ID |
307072 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Fmo9
|
| Ensembl Gene |
ENSMUSG00000026560 |
| Gene Name |
flavin containing monooxygenase 9 |
| Synonyms |
4831428F09Rik |
| MMRRC Submission |
040915-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.063)
|
| Stock # |
R3924 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
1 |
| Chromosomal Location |
166489624-166509414 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 166492221 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Serine to Proline
at position 350
(S350P)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000114750
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000027843]
[ENSMUST00000148677]
|
| AlphaFold |
Q8C116 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000027843
AA Change: S401P
PolyPhen 2
Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)
|
| SMART Domains |
Protein: ENSMUSP00000027843
Gene: ENSMUSG00000026560
AA Change: S401P
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
3 |
535 |
1.2e-252 |
PFAM |
|
Pfam:Pyr_redox_2
|
4 |
262 |
2.9e-12 |
PFAM |
|
Pfam:Pyr_redox_3
|
7 |
221 |
2.3e-14 |
PFAM |
|
Pfam:NAD_binding_8
|
8 |
83 |
1.2e-6 |
PFAM |
|
Pfam:K_oxygenase
|
77 |
334 |
8.9e-11 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000148677
AA Change: S350P
PolyPhen 2
Score 0.030 (Sensitivity: 0.95; Specificity: 0.82)
|
| SMART Domains |
Protein: ENSMUSP00000114750
Gene: ENSMUSG00000026560
AA Change: S350P
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:FMO-like
|
1 |
484 |
1.6e-222 |
PFAM |
|
Pfam:Pyr_redox_3
|
3 |
170 |
1.2e-12 |
PFAM |
|
Pfam:K_oxygenase
|
28 |
283 |
6.2e-11 |
PFAM |
|
| Meta Mutation Damage Score |
0.0898 |
| Coding Region Coverage |
- 1x: 99.3%
- 3x: 98.7%
- 10x: 97.5%
- 20x: 95.8%
|
| Validation Efficiency |
98% (39/40) |
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 39 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4930550C14Rik |
G |
T |
9: 53,343,705 (GRCm39) |
V119F |
probably benign |
Het |
| Agtpbp1 |
A |
T |
13: 59,648,221 (GRCm39) |
V533D |
probably benign |
Het |
| Ahnak |
G |
A |
19: 8,983,692 (GRCm39) |
D1659N |
probably benign |
Het |
| Aldh3b3 |
A |
G |
19: 4,018,491 (GRCm39) |
N402S |
probably damaging |
Het |
| Amn |
T |
C |
12: 111,242,114 (GRCm39) |
V367A |
possibly damaging |
Het |
| Arpin |
A |
G |
7: 79,579,435 (GRCm39) |
Y63H |
probably benign |
Het |
| Brinp2 |
A |
G |
1: 158,073,778 (GRCm39) |
L781P |
probably damaging |
Het |
| Cdkl1 |
G |
T |
12: 69,803,373 (GRCm39) |
R168S |
probably damaging |
Het |
| Cfap43 |
T |
C |
19: 47,785,555 (GRCm39) |
K445R |
probably benign |
Het |
| Csad |
T |
C |
15: 102,086,991 (GRCm39) |
S427G |
probably benign |
Het |
| Dach1 |
A |
G |
14: 98,153,339 (GRCm39) |
V443A |
probably damaging |
Het |
| Dlat |
A |
G |
9: 50,569,490 (GRCm39) |
S160P |
possibly damaging |
Het |
| Dpf1 |
A |
G |
7: 29,011,098 (GRCm39) |
R165G |
possibly damaging |
Het |
| Dtd2 |
G |
C |
12: 52,051,734 (GRCm39) |
|
probably null |
Het |
| Fa2h |
A |
G |
8: 112,120,147 (GRCm39) |
Y80H |
probably damaging |
Het |
| Flii |
A |
G |
11: 60,610,902 (GRCm39) |
F509S |
probably damaging |
Het |
| Gabra4 |
A |
G |
5: 71,799,596 (GRCm39) |
|
probably benign |
Het |
| Gm3604 |
A |
G |
13: 62,518,044 (GRCm39) |
S105P |
probably damaging |
Het |
| Gpr155 |
T |
A |
2: 73,200,420 (GRCm39) |
L362F |
probably damaging |
Het |
| Lmbrd2 |
T |
C |
15: 9,149,624 (GRCm39) |
V86A |
probably benign |
Het |
| Lpcat4 |
G |
T |
2: 112,077,061 (GRCm39) |
Q468H |
possibly damaging |
Het |
| Luzp1 |
T |
C |
4: 136,270,168 (GRCm39) |
I797T |
probably damaging |
Het |
| Myh8 |
A |
T |
11: 67,187,963 (GRCm39) |
I912F |
probably damaging |
Het |
| Notch2 |
G |
T |
3: 98,029,350 (GRCm39) |
G1038* |
probably null |
Het |
| Nptx1 |
G |
T |
11: 119,438,333 (GRCm39) |
T28N |
possibly damaging |
Het |
| Onecut2 |
A |
G |
18: 64,474,591 (GRCm39) |
K381E |
probably damaging |
Het |
| Or9i2 |
T |
C |
19: 13,816,130 (GRCm39) |
T136A |
probably damaging |
Het |
| Plekha5 |
A |
G |
6: 140,516,105 (GRCm39) |
N317S |
possibly damaging |
Het |
| Polr1a |
A |
G |
6: 71,906,434 (GRCm39) |
M417V |
probably benign |
Het |
| Ptpn13 |
T |
C |
5: 103,698,607 (GRCm39) |
|
probably benign |
Het |
| Qrfpr |
T |
C |
3: 36,276,072 (GRCm39) |
N106S |
possibly damaging |
Het |
| Rasd2 |
A |
G |
8: 75,948,602 (GRCm39) |
N176S |
probably damaging |
Het |
| Rsbn1l |
A |
G |
5: 21,124,785 (GRCm39) |
V339A |
probably damaging |
Het |
| Ryr3 |
A |
G |
2: 112,859,048 (GRCm39) |
|
probably benign |
Het |
| Shkbp1 |
A |
G |
7: 27,041,827 (GRCm39) |
W676R |
probably benign |
Het |
| Sipa1 |
G |
A |
19: 5,710,407 (GRCm39) |
T201I |
probably benign |
Het |
| Slc35g2 |
A |
T |
9: 100,434,780 (GRCm39) |
I297N |
probably benign |
Het |
| Usp28 |
T |
C |
9: 48,942,223 (GRCm39) |
|
probably null |
Het |
| Zfp946 |
G |
T |
17: 22,674,682 (GRCm39) |
G479C |
probably benign |
Het |
|
| Other mutations in Fmo9 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00886:Fmo9
|
APN |
1 |
166,507,714 (GRCm39) |
splice site |
probably null |
|
|
IGL01796:Fmo9
|
APN |
1 |
166,490,904 (GRCm39) |
missense |
probably benign |
0.31 |
|
IGL03005:Fmo9
|
APN |
1 |
166,502,088 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL03115:Fmo9
|
APN |
1 |
166,505,220 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03163:Fmo9
|
APN |
1 |
166,502,019 (GRCm39) |
missense |
possibly damaging |
0.77 |
|
R0089:Fmo9
|
UTSW |
1 |
166,494,878 (GRCm39) |
missense |
probably benign |
0.05 |
|
R0570:Fmo9
|
UTSW |
1 |
166,502,031 (GRCm39) |
missense |
probably null |
0.00 |
|
R1520:Fmo9
|
UTSW |
1 |
166,495,024 (GRCm39) |
missense |
probably benign |
0.19 |
|
R1779:Fmo9
|
UTSW |
1 |
166,490,868 (GRCm39) |
missense |
probably benign |
0.18 |
|
R1783:Fmo9
|
UTSW |
1 |
166,501,217 (GRCm39) |
missense |
probably benign |
0.01 |
|
R2858:Fmo9
|
UTSW |
1 |
166,501,236 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2859:Fmo9
|
UTSW |
1 |
166,501,236 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3851:Fmo9
|
UTSW |
1 |
166,490,936 (GRCm39) |
missense |
probably benign |
0.00 |
|
R4470:Fmo9
|
UTSW |
1 |
166,507,799 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4728:Fmo9
|
UTSW |
1 |
166,490,880 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R5538:Fmo9
|
UTSW |
1 |
166,501,198 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5650:Fmo9
|
UTSW |
1 |
166,491,015 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5820:Fmo9
|
UTSW |
1 |
166,492,170 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R6163:Fmo9
|
UTSW |
1 |
166,494,962 (GRCm39) |
missense |
probably benign |
|
|
R6229:Fmo9
|
UTSW |
1 |
166,505,126 (GRCm39) |
missense |
possibly damaging |
0.64 |
|
R6243:Fmo9
|
UTSW |
1 |
166,494,938 (GRCm39) |
missense |
probably benign |
0.45 |
|
R6375:Fmo9
|
UTSW |
1 |
166,492,164 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7144:Fmo9
|
UTSW |
1 |
166,505,189 (GRCm39) |
missense |
probably benign |
0.40 |
|
R7236:Fmo9
|
UTSW |
1 |
166,504,140 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7316:Fmo9
|
UTSW |
1 |
166,491,215 (GRCm39) |
missense |
probably benign |
0.21 |
|
R7341:Fmo9
|
UTSW |
1 |
166,504,115 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7382:Fmo9
|
UTSW |
1 |
166,491,229 (GRCm39) |
splice site |
probably null |
|
|
R7589:Fmo9
|
UTSW |
1 |
166,501,997 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R7679:Fmo9
|
UTSW |
1 |
166,495,058 (GRCm39) |
missense |
probably benign |
0.01 |
|
R8110:Fmo9
|
UTSW |
1 |
166,491,095 (GRCm39) |
missense |
probably benign |
0.03 |
|
R8500:Fmo9
|
UTSW |
1 |
166,502,039 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9098:Fmo9
|
UTSW |
1 |
166,492,199 (GRCm39) |
missense |
possibly damaging |
0.48 |
|
R9301:Fmo9
|
UTSW |
1 |
166,494,794 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9400:Fmo9
|
UTSW |
1 |
166,505,243 (GRCm39) |
missense |
probably benign |
0.09 |
|
R9401:Fmo9
|
UTSW |
1 |
166,505,189 (GRCm39) |
missense |
probably damaging |
0.99 |
|
Z1088:Fmo9
|
UTSW |
1 |
166,501,114 (GRCm39) |
critical splice donor site |
probably null |
|
|
| Predicted Primers |
PCR Primer
(F):5'- GGGAGGATAAATTCACACTCAATCAC -3'
(R):5'- TTCTGAGGAACCACCACACTG -3'
Sequencing Primer
(F):5'- TCAATCACCACTGGACTGGTC -3'
(R):5'- ACTGATTTCCATACTGGCTCTACAAG -3'
|
| Posted On |
2015-04-17 |
Incidental Mutation