Mutagenetix > Incidental Mutation

Incidental Mutation 'R4323:Itsn1'

323929

Institutional Source

Beutler Lab

Gene Symbol

Itsn1

Ensembl Gene

ENSMUSG00000022957

Gene Name

intersectin 1 (SH3 domain protein 1A)

Synonyms

Eh domain, SH3 domain regulator of endocytosis 1, Sh3p17, Ese1, Intersectin-L, EHSH1

MMRRC Submission

041094-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4323 (G1)

Quality Score

158

Status

Validated

Chromosome

Chromosomal Location

91526198-91717479 bp(+) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

G to A at 91615440 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000125172 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000056482] [ENSMUST00000064797] [ENSMUST00000095909] [ENSMUST00000113993] [ENSMUST00000113996] [ENSMUST00000113999] [ENSMUST00000114001] [ENSMUST00000114002] [ENSMUST00000135057] [ENSMUST00000159295]

AlphaFold

Q9Z0R4

Predicted Effect

unknown
Transcript: ENSMUST00000056482
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000056011
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	661	N/A	INTRINSIC
low complexity region	687	696	N/A	INTRINSIC
SH3	741	798	1.05e-19	SMART
SH3	909	963	2.64e-16	SMART
SH3	998	1052	1.82e-19	SMART
SH3	1070	1130	2.46e-16	SMART
SH3	1151	1206	7.97e-25	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000064797
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000066361
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	661	N/A	INTRINSIC
low complexity region	687	696	N/A	INTRINSIC
SH3	741	803	1.62e-16	SMART
SH3	914	968	2.64e-16	SMART
SH3	1003	1057	1.82e-19	SMART
SH3	1075	1135	2.46e-16	SMART
SH3	1156	1211	7.97e-25	SMART
RhoGEF	1239	1420	1e-63	SMART
PH	1461	1571	6.07e-13	SMART
C2	1595	1692	1.58e-18	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000095909
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000093598
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	607	N/A	INTRINSIC

Predicted Effect

unknown
Transcript: ENSMUST00000113993
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000109626
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	661	N/A	INTRINSIC
low complexity region	687	696	N/A	INTRINSIC
SH3	741	803	1.62e-16	SMART
SH3	914	968	2.64e-16	SMART
SH3	1004	1064	2.46e-16	SMART
SH3	1085	1140	7.97e-25	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000113996
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000109629
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	661	N/A	INTRINSIC
low complexity region	687	696	N/A	INTRINSIC
SH3	741	798	1.05e-19	SMART
SH3	909	963	2.64e-16	SMART
SH3	999	1059	2.46e-16	SMART
SH3	1080	1135	7.97e-25	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000113999
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000109632
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	661	N/A	INTRINSIC
low complexity region	687	696	N/A	INTRINSIC
SH3	741	803	1.62e-16	SMART
SH3	914	968	2.64e-16	SMART
SH3	1003	1057	1.82e-19	SMART
SH3	1075	1135	2.46e-16	SMART
SH3	1156	1211	7.97e-25	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000114001
AA Change: R358Q

SMART Domains

Protein: ENSMUSP00000109634
Gene: ENSMUSG00000022957
AA Change: R358Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	145	155	N/A	INTRINSIC
EH	177	272	2.55e-49	SMART
EFh	221	249	1.77e-2	SMART
low complexity region	293	305	N/A	INTRINSIC
coiled coil region	315	410	N/A	INTRINSIC
coiled coil region	431	478	N/A	INTRINSIC
low complexity region	489	500	N/A	INTRINSIC
coiled coil region	524	624	N/A	INTRINSIC
low complexity region	650	659	N/A	INTRINSIC
SH3	704	761	1.05e-19	SMART
SH3	872	926	2.64e-16	SMART
SH3	961	1015	1.82e-19	SMART
SH3	1033	1093	2.46e-16	SMART
SH3	1114	1169	7.97e-25	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151044

Predicted Effect

unknown
Transcript: ENSMUST00000114002
AA Change: R395Q

SMART Domains

Protein: ENSMUSP00000109635
Gene: ENSMUSG00000022957
AA Change: R395Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	138	165	N/A	INTRINSIC
low complexity region	182	192	N/A	INTRINSIC
EH	214	309	2.55e-49	SMART
EFh	258	286	1.77e-2	SMART
low complexity region	330	342	N/A	INTRINSIC
coiled coil region	352	447	N/A	INTRINSIC
coiled coil region	468	515	N/A	INTRINSIC
low complexity region	526	537	N/A	INTRINSIC
coiled coil region	561	661	N/A	INTRINSIC
low complexity region	687	696	N/A	INTRINSIC
SH3	741	798	1.05e-19	SMART
SH3	909	963	2.64e-16	SMART
SH3	998	1052	1.82e-19	SMART
SH3	1070	1130	2.46e-16	SMART
SH3	1151	1206	7.97e-25	SMART
RhoGEF	1234	1415	1e-63	SMART
PH	1456	1566	6.07e-13	SMART
C2	1590	1687	1.58e-18	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000135057
AA Change: R334Q

SMART Domains

Protein: ENSMUSP00000117018
Gene: ENSMUSG00000022957
AA Change: R334Q

Domain	Start	End	E-Value	Type
EH	14	108	1.34e-43	SMART
EFh	57	85	2.14e-1	SMART
low complexity region	121	131	N/A	INTRINSIC
EH	153	248	2.55e-49	SMART
EFh	197	225	1.77e-2	SMART
low complexity region	269	281	N/A	INTRINSIC
coiled coil region	291	359	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124094

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000133389

Predicted Effect

probably benign
Transcript: ENSMUST00000159295

SMART Domains

Protein: ENSMUSP00000125172
Gene: ENSMUSG00000116933

Domain	Start	End	E-Value	Type
Pfam:OSCP	1	89	1.1e-16	PFAM

Meta Mutation Damage Score

0.1872

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

95% (59/62)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous for a gene trapped allele exhibit embryonic lethal. Mice homozygous for a null allele exhibit some postnatal lethality and impaired vesicle recycling in surviving mice. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933430I17Rik	A	G	4: 62,465,548 (GRCm39)	Y414C	probably damaging	Het
Akr1b1	T	C	6: 34,287,862 (GRCm39)	T166A	probably benign	Het
Ankhd1	C	T	18: 36,711,686 (GRCm39)	S94L	probably damaging	Het
B4galt3	G	T	1: 171,103,515 (GRCm39)	M68I	possibly damaging	Het
Bpnt1	A	C	1: 185,088,786 (GRCm39)	H312P	probably benign	Het
Ccdc178	T	A	18: 22,166,600 (GRCm39)	K530*	probably null	Het
Ccdc191	A	G	16: 43,767,872 (GRCm39)	E624G	probably damaging	Het
Clasp2	T	C	9: 113,719,027 (GRCm39)	V724A	possibly damaging	Het
Copa	G	T	1: 171,946,831 (GRCm39)	C1022F	probably damaging	Het
Cwf19l2	T	G	9: 3,430,452 (GRCm39)	F261L	probably damaging	Het
Esr1	G	A	10: 4,951,307 (GRCm39)	V562M	possibly damaging	Het
Fap	T	C	2: 62,333,716 (GRCm39)	H643R	probably damaging	Het
Fbxo38	T	A	18: 62,648,232 (GRCm39)	M769L	probably benign	Het
Fgfr1	A	G	8: 26,063,915 (GRCm39)	N814S	probably benign	Het
Hipk3	C	A	2: 104,276,916 (GRCm39)	V388L	probably damaging	Het
Hspa1l	T	A	17: 35,196,832 (GRCm39)	Y290*	probably null	Het
Jam2	T	C	16: 84,619,744 (GRCm39)		probably benign	Het
Kprp	G	A	3: 92,732,163 (GRCm39)	R296W	probably damaging	Het
Med23	T	C	10: 24,746,603 (GRCm39)	I14T	probably benign	Het
Mitf	A	G	6: 97,968,910 (GRCm39)	Y10C	probably benign	Het
Mpo	T	C	11: 87,686,865 (GRCm39)	S165P	probably damaging	Het
Neb	T	G	2: 52,154,122 (GRCm39)	M2330L	possibly damaging	Het
Nup214	T	C	2: 31,884,696 (GRCm39)	S486P	probably benign	Het
Or2y1f	G	A	11: 49,184,503 (GRCm39)	M118I	probably damaging	Het
Parp6	G	A	9: 59,537,969 (GRCm39)	V205I	possibly damaging	Het
Pate2	A	T	9: 35,581,767 (GRCm39)		probably benign	Het
Pdss1	T	C	2: 22,802,608 (GRCm39)		probably benign	Het
Pira13	A	G	7: 3,825,754 (GRCm39)	S372P	possibly damaging	Het
Rufy4	T	C	1: 74,186,822 (GRCm39)	C537R	probably damaging	Het
Septin1	G	A	7: 126,816,200 (GRCm39)	P77S	probably damaging	Het
Slitrk3	A	G	3: 72,958,118 (GRCm39)	L218P	probably damaging	Het
Sltm	T	C	9: 70,487,529 (GRCm39)	I521T	probably benign	Het
Smchd1	A	G	17: 71,735,270 (GRCm39)	I618T	probably benign	Het
Sox6	A	G	7: 115,179,798 (GRCm39)		probably null	Het
Sp8	A	G	12: 118,812,171 (GRCm39)	I9V	probably benign	Het
Usp9y	T	A	Y: 1,434,407 (GRCm39)	M352L	possibly damaging	Het
Vmn1r46	T	C	6: 89,953,349 (GRCm39)	M66T	probably benign	Het
Vmn2r111	A	T	17: 22,792,159 (GRCm39)	N32K	probably benign	Het
Vmn2r63	A	G	7: 42,576,406 (GRCm39)	F469S	probably benign	Het
Vps13d	T	A	4: 144,879,348 (GRCm39)	T1486S	probably benign	Het
Wdr55	A	G	18: 36,896,153 (GRCm39)	N281S	probably benign	Het
Zswim6	G	A	13: 108,025,938 (GRCm39)		noncoding transcript	Het

Other mutations in Itsn1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01109:Itsn1	APN	16	91,603,089 (GRCm39)	unclassified	probably benign
IGL01799:Itsn1	APN	16	91,645,770 (GRCm39)	missense	probably damaging	1.00
IGL02328:Itsn1	APN	16	91,612,295 (GRCm39)	missense	probably damaging	1.00
IGL02333:Itsn1	APN	16	91,617,564 (GRCm39)	intron	probably benign
IGL02503:Itsn1	APN	16	91,686,092 (GRCm39)	missense	possibly damaging	0.62
IGL02628:Itsn1	APN	16	91,696,511 (GRCm39)	missense	possibly damaging	0.79
IGL02666:Itsn1	APN	16	91,617,606 (GRCm39)	intron	probably benign
IGL03007:Itsn1	APN	16	91,581,050 (GRCm39)	splice site	probably benign
IGL03223:Itsn1	APN	16	91,702,194 (GRCm39)	missense	probably benign	0.00
raphael	UTSW	16	91,617,684 (GRCm39)	intron	probably benign
Sector	UTSW	16	91,705,375 (GRCm39)	critical splice donor site	probably null
Weevil	UTSW	16	91,615,440 (GRCm39)	intron	probably benign
R0234:Itsn1	UTSW	16	91,625,168 (GRCm39)	nonsense	probably null
R0234:Itsn1	UTSW	16	91,625,168 (GRCm39)	nonsense	probably null
R0255:Itsn1	UTSW	16	91,602,978 (GRCm39)	unclassified	probably benign
R0432:Itsn1	UTSW	16	91,612,408 (GRCm39)	missense	probably damaging	1.00
R0455:Itsn1	UTSW	16	91,665,036 (GRCm39)	intron	probably benign
R0471:Itsn1	UTSW	16	91,696,477 (GRCm39)	missense	probably damaging	1.00
R0558:Itsn1	UTSW	16	91,696,511 (GRCm39)	missense	possibly damaging	0.79
R0563:Itsn1	UTSW	16	91,617,684 (GRCm39)	intron	probably benign
R1657:Itsn1	UTSW	16	91,706,111 (GRCm39)	missense	probably damaging	1.00
R1671:Itsn1	UTSW	16	91,609,038 (GRCm39)	missense	probably damaging	1.00
R1742:Itsn1	UTSW	16	91,613,847 (GRCm39)	critical splice donor site	probably null
R1859:Itsn1	UTSW	16	91,686,042 (GRCm39)	intron	probably benign
R1898:Itsn1	UTSW	16	91,696,468 (GRCm39)	missense	probably damaging	1.00
R2016:Itsn1	UTSW	16	91,702,389 (GRCm39)	critical splice donor site	probably null
R2221:Itsn1	UTSW	16	91,650,656 (GRCm39)	intron	probably benign
R2244:Itsn1	UTSW	16	91,650,659 (GRCm39)	missense	probably null
R3160:Itsn1	UTSW	16	91,649,932 (GRCm39)	nonsense	probably null
R3162:Itsn1	UTSW	16	91,649,932 (GRCm39)	nonsense	probably null
R3814:Itsn1	UTSW	16	91,649,809 (GRCm39)	missense	possibly damaging	0.96
R4162:Itsn1	UTSW	16	91,649,790 (GRCm39)	missense	probably benign	0.00
R4254:Itsn1	UTSW	16	91,615,440 (GRCm39)	intron	probably benign
R4319:Itsn1	UTSW	16	91,615,440 (GRCm39)	intron	probably benign
R4321:Itsn1	UTSW	16	91,615,440 (GRCm39)	intron	probably benign
R4326:Itsn1	UTSW	16	91,650,743 (GRCm39)	intron	probably benign
R4515:Itsn1	UTSW	16	91,696,537 (GRCm39)	missense	probably damaging	0.99
R4584:Itsn1	UTSW	16	91,617,471 (GRCm39)	intron	probably benign
R4600:Itsn1	UTSW	16	91,696,475 (GRCm39)	missense	probably damaging	1.00
R4649:Itsn1	UTSW	16	91,638,476 (GRCm39)	missense	probably damaging	1.00
R4834:Itsn1	UTSW	16	91,703,677 (GRCm39)	nonsense	probably null
R4868:Itsn1	UTSW	16	91,582,205 (GRCm39)	missense	probably damaging	0.98
R5036:Itsn1	UTSW	16	91,579,123 (GRCm39)	splice site	probably benign
R5122:Itsn1	UTSW	16	91,690,732 (GRCm39)	intron	probably benign
R5161:Itsn1	UTSW	16	91,705,726 (GRCm39)	missense	possibly damaging	0.95
R5437:Itsn1	UTSW	16	91,615,479 (GRCm39)	intron	probably benign
R5538:Itsn1	UTSW	16	91,580,990 (GRCm39)	missense	probably damaging	1.00
R5683:Itsn1	UTSW	16	91,702,268 (GRCm39)	missense	probably benign	0.00
R5697:Itsn1	UTSW	16	91,598,477 (GRCm39)	missense	possibly damaging	0.56
R5749:Itsn1	UTSW	16	91,703,743 (GRCm39)	missense	probably damaging	0.99
R6083:Itsn1	UTSW	16	91,649,899 (GRCm39)	missense	probably benign	0.01
R6148:Itsn1	UTSW	16	91,613,740 (GRCm39)	missense	probably damaging	1.00
R6291:Itsn1	UTSW	16	91,664,984 (GRCm39)	intron	probably benign
R6524:Itsn1	UTSW	16	91,708,883 (GRCm39)	missense	probably damaging	0.96
R7175:Itsn1	UTSW	16	91,664,938 (GRCm39)	missense	unknown
R7261:Itsn1	UTSW	16	91,702,194 (GRCm39)	missense	probably benign	0.00
R7320:Itsn1	UTSW	16	91,636,587 (GRCm39)	missense	unknown
R7366:Itsn1	UTSW	16	91,705,338 (GRCm39)	missense	unknown
R7462:Itsn1	UTSW	16	91,650,073 (GRCm39)	missense	possibly damaging	0.53
R7665:Itsn1	UTSW	16	91,638,491 (GRCm39)	missense	unknown
R7720:Itsn1	UTSW	16	91,664,971 (GRCm39)	missense	unknown
R7856:Itsn1	UTSW	16	91,705,375 (GRCm39)	critical splice donor site	probably null
R7864:Itsn1	UTSW	16	91,598,454 (GRCm39)	missense	possibly damaging	0.47
R7896:Itsn1	UTSW	16	91,615,446 (GRCm39)	missense	unknown
R7897:Itsn1	UTSW	16	91,615,446 (GRCm39)	missense	unknown
R7980:Itsn1	UTSW	16	91,702,182 (GRCm39)	missense	unknown
R7998:Itsn1	UTSW	16	91,647,824 (GRCm39)	missense	unknown
R8075:Itsn1	UTSW	16	91,686,097 (GRCm39)	missense	unknown
R8144:Itsn1	UTSW	16	91,708,893 (GRCm39)	missense	unknown
R8160:Itsn1	UTSW	16	91,615,446 (GRCm39)	missense	unknown
R8161:Itsn1	UTSW	16	91,615,446 (GRCm39)	missense	unknown
R8176:Itsn1	UTSW	16	91,645,597 (GRCm39)	splice site	probably null
R8215:Itsn1	UTSW	16	91,608,996 (GRCm39)	missense	probably damaging	0.99
R8385:Itsn1	UTSW	16	91,690,699 (GRCm39)	missense	unknown
R8725:Itsn1	UTSW	16	91,636,721 (GRCm39)	missense	unknown
R9012:Itsn1	UTSW	16	91,645,849 (GRCm39)	missense	unknown
R9039:Itsn1	UTSW	16	91,703,658 (GRCm39)	missense	unknown
R9092:Itsn1	UTSW	16	91,609,002 (GRCm39)	missense	possibly damaging	0.86
R9134:Itsn1	UTSW	16	91,666,514 (GRCm39)	missense	unknown
R9401:Itsn1	UTSW	16	91,612,408 (GRCm39)	missense	probably damaging	1.00
R9449:Itsn1	UTSW	16	91,625,264 (GRCm39)	makesense	probably null
R9568:Itsn1	UTSW	16	91,649,782 (GRCm39)	missense	probably benign
R9616:Itsn1	UTSW	16	91,650,055 (GRCm39)	missense	probably benign	0.11

Predicted Primers

PCR Primer
(F):5'- AATGACTTTCCCATCGCTGGTG -3'
(R):5'- GTGAAGACTGAGCAACTCTGG -3'

Sequencing Primer
(F):5'- CCATCGCTGGTGTGTCC -3'
(R):5'- TGAAGACTGAGCAACTCTGGATACC -3'

Posted On

2015-06-24