Incidental Mutation 'R4330:Cnr2'
ID 324561
Institutional Source Beutler Lab
Gene Symbol Cnr2
Ensembl Gene ENSMUSG00000062585
Gene Name cannabinoid receptor 2
Synonyms cannabinoid receptor 2 (spleen), CB2-R, CB2
Accession Numbers
Essential gene? Probably non essential (E-score: 0.072) question?
Stock # R4330 (G1)
Quality Score 225
Status Not validated
Chromosome 4
Chromosomal Location 135622705-135647518 bp(+) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to A at 135644237 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Isoleucine to Asparagine at position 105 (I105N)
Ref Sequence ENSEMBL: ENSMUSP00000095454 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000030434] [ENSMUST00000068830] [ENSMUST00000097843]
AlphaFold P47936
Predicted Effect probably benign
Transcript: ENSMUST00000030434
SMART Domains Protein: ENSMUSP00000030434
Gene: ENSMUSG00000028673

DomainStartEndE-ValueType
low complexity region 2 19 N/A INTRINSIC
Alpha_L_fucos 22 399 7.97e-234 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000068830
AA Change: I105N

PolyPhen 2 Score 0.557 (Sensitivity: 0.88; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000069957
Gene: ENSMUSG00000062585
AA Change: I105N

DomainStartEndE-ValueType
Pfam:7tm_1 50 299 1.8e-44 PFAM
Predicted Effect possibly damaging
Transcript: ENSMUST00000097843
AA Change: I105N

PolyPhen 2 Score 0.557 (Sensitivity: 0.88; Specificity: 0.91)
SMART Domains Protein: ENSMUSP00000095454
Gene: ENSMUSG00000062585
AA Change: I105N

DomainStartEndE-ValueType
Pfam:7tm_1 50 299 8e-37 PFAM
Coding Region Coverage
  • 1x: 99.3%
  • 3x: 98.7%
  • 10x: 97.5%
  • 20x: 95.8%
Validation Efficiency
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]
PHENOTYPE: Macrophages from homozygous mutant animals are resistant to the inhibitory effects of delta9-Tetrahydrocannabinol. Alopecia is seen in some but not all homozygotes. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 23 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Adam34l T A 8: 44,079,287 (GRCm39) K312N probably benign Het
Arl6ip1 AAAATAAATAAATAAATAAATAAATA AAAATAAATAAATAAATAAATAAATAAATA 7: 117,721,122 (GRCm39) probably benign Het
Cdh10 G A 15: 19,000,045 (GRCm39) V497I probably damaging Het
Chd4 T C 6: 125,078,565 (GRCm39) V229A probably benign Het
Dnai1 A G 4: 41,637,966 (GRCm39) T701A probably damaging Het
Eri1 G A 8: 35,936,383 (GRCm39) R313* probably null Het
Hrh3 T C 2: 179,741,665 (GRCm39) probably benign Het
Hs3st5 T A 10: 36,708,726 (GRCm39) V87D probably benign Het
Jmy G C 13: 93,635,390 (GRCm39) P142R probably damaging Het
Jmy C A 13: 93,635,781 (GRCm39) D12Y probably damaging Het
Mfsd4a A C 1: 131,981,291 (GRCm39) M320R possibly damaging Het
Myocd A T 11: 65,114,590 (GRCm39) H49Q probably benign Het
Nlk A G 11: 78,481,774 (GRCm39) I229T possibly damaging Het
Rabl2 T C 15: 89,471,137 (GRCm39) D66G probably benign Het
Ramp1 C T 1: 91,151,067 (GRCm39) T144I possibly damaging Het
Rhbdf2 T C 11: 116,492,782 (GRCm39) K413R probably benign Het
Scpep1 G A 11: 88,826,729 (GRCm39) Q236* probably null Het
Stil A G 4: 114,862,176 (GRCm39) R40G probably damaging Het
Syt7 T C 19: 10,399,162 (GRCm39) V86A probably damaging Het
Ubr2 A T 17: 47,278,204 (GRCm39) L711Q probably null Het
Vmn1r78 T A 7: 11,886,386 (GRCm39) probably null Het
Zfp280d C T 9: 72,203,261 (GRCm39) T3I possibly damaging Het
Zscan25 T A 5: 145,227,361 (GRCm39) F342I probably damaging Het
Other mutations in Cnr2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01990:Cnr2 APN 4 135,644,116 (GRCm39) missense probably damaging 1.00
IGL02233:Cnr2 APN 4 135,644,522 (GRCm39) missense possibly damaging 0.76
IGL02887:Cnr2 APN 4 135,644,936 (GRCm39) missense possibly damaging 0.90
PIT4791001:Cnr2 UTSW 4 135,644,263 (GRCm39) missense probably damaging 1.00
R0480:Cnr2 UTSW 4 135,644,912 (GRCm39) missense probably benign 0.00
R0616:Cnr2 UTSW 4 135,644,873 (GRCm39) missense probably benign 0.39
R0945:Cnr2 UTSW 4 135,644,632 (GRCm39) missense probably benign 0.16
R1242:Cnr2 UTSW 4 135,644,294 (GRCm39) missense probably damaging 1.00
R1538:Cnr2 UTSW 4 135,644,012 (GRCm39) missense probably benign 0.00
R4911:Cnr2 UTSW 4 135,644,512 (GRCm39) missense possibly damaging 0.88
R4960:Cnr2 UTSW 4 135,644,918 (GRCm39) missense probably benign 0.01
R5289:Cnr2 UTSW 4 135,644,318 (GRCm39) missense probably damaging 1.00
R5642:Cnr2 UTSW 4 135,644,076 (GRCm39) missense probably damaging 1.00
R5681:Cnr2 UTSW 4 135,644,000 (GRCm39) missense probably damaging 0.99
R5807:Cnr2 UTSW 4 135,644,747 (GRCm39) missense probably benign 0.00
R6843:Cnr2 UTSW 4 135,644,900 (GRCm39) missense probably benign
R8024:Cnr2 UTSW 4 135,644,196 (GRCm39) missense probably damaging 1.00
R8423:Cnr2 UTSW 4 135,644,827 (GRCm39) missense probably damaging 1.00
R9330:Cnr2 UTSW 4 135,644,312 (GRCm39) missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- ACATGATCCTGAGCAGTGGC -3'
(R):5'- ATGCTGAGAGGACCCACATG -3'

Sequencing Primer
(F):5'- TGCTGTGCACCCTGATG -3'
(R):5'- TGACACAGAGGGCCACCAG -3'
Posted On 2015-06-24