Mutagenetix > Incidental Mutations

Incidental Mutation 'R5761:Gpr89'

445284

Institutional Source

Beutler Lab

Gene Symbol

Gpr89

Ensembl Gene

ENSMUSG00000028096

Gene Name

G protein-coupled receptor 89

Synonyms

4933412D19Rik, SH120

MMRRC Submission

043363-MU

Accession Numbers

Is this an essential gene?

Possibly non essential (E-score: 0.326) question?

Stock #

R5761 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

96868281-96905346 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 96892880 bp

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 134 (L134P)

Ref Sequence

ENSEMBL: ENSMUSP00000029738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029738] [ENSMUST00000154750]

Predicted Effect

probably damaging
Transcript: ENSMUST00000029738
AA Change: L134P

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000029738
Gene: ENSMUSG00000028096
AA Change: L134P

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC
transmembrane domain	110	127	N/A	INTRINSIC
Pfam:GPHR_N	140	207	1.1e-31	PFAM
Pfam:ABA_GPCR	276	446	4.9e-46	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139599

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146645

Predicted Effect

probably benign
Transcript: ENSMUST00000154750

SMART Domains

Protein: ENSMUSP00000116016
Gene: ENSMUSG00000028096

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199586

Meta Mutation Damage Score

0.6224

Coding Region Coverage

1x: 99.5%
3x: 98.8%
10x: 97.3%
20x: 95.2%

Validation Efficiency

99% (67/68)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	A	T	11: 110,210,101	V953D	probably damaging	Het
Abcb8	T	C	5: 24,405,881		probably benign	Het
Acad12	A	T	5: 121,604,180		probably benign	Het
Adam2	A	T	14: 66,046,146	C436S	probably damaging	Het
Aebp2	T	C	6: 140,624,217		probably benign	Het
AI314180	T	C	4: 58,853,131	I401M	probably damaging	Het
Akap8	A	T	17: 32,317,185	C85S	possibly damaging	Het
Aldh1a3	C	A	7: 66,419,179	R19L	probably damaging	Het
Baz2a	A	G	10: 128,119,690	T848A	possibly damaging	Het
Bud13	C	T	9: 46,288,052	A237V	probably benign	Het
Cbarp	CGCCTCTGCTGCCTCT	CGCCTCT	10: 80,132,233		probably benign	Het
Ccdc187	G	A	2: 26,276,092	P775L	possibly damaging	Het
Ccz1	C	T	5: 143,992,510	G367R	probably damaging	Het
Cep104	T	C	4: 153,981,224	V56A	possibly damaging	Het
Chd6	C	A	2: 160,957,078	R2362S	probably damaging	Het
Chd6	C	T	2: 160,957,079	R2362K	probably damaging	Het
Cmc1	T	C	9: 118,115,375	E25G	probably benign	Het
Cntnap5b	T	C	1: 100,446,894	S1123P	probably damaging	Het
Col4a3	T	A	1: 82,716,057	L66*	probably null	Het
Crmp1	A	G	5: 37,282,868	T329A	probably benign	Het
Cybb	C	G	X: 9,450,750	D246H	probably benign	Het
Cyp3a16	A	G	5: 145,442,033	S393P	possibly damaging	Het
Cyp4f18	T	A	8: 71,996,131	I225F	probably damaging	Het
Ddhd2	A	G	8: 25,741,699	V432A	probably benign	Het
Foxq1	G	A	13: 31,559,331	A139T	probably damaging	Het
Gm10428	G	T	11: 62,753,343		probably benign	Het
Hfe2	T	C	3: 96,528,622	S399P	probably benign	Het
Hrc	A	T	7: 45,336,601		probably null	Het
Igf1r	T	A	7: 68,207,253	Y988N	probably damaging	Het
Igf2r	A	C	17: 12,698,352		probably null	Het
Itga2b	A	G	11: 102,466,274	F260S	probably benign	Het
Kif2a	T	A	13: 106,962,164	N698I	probably benign	Het
Lap3	A	C	5: 45,504,805	I316L	probably benign	Het
Map2k6	T	G	11: 110,399,371		probably benign	Het
Myh1	A	G	11: 67,219,252	E1422G	probably damaging	Het
Ncoa6	A	G	2: 155,408,141	V1081A	probably benign	Het
Nom1	A	G	5: 29,437,641	E380G	probably damaging	Het
Nwd2	A	T	5: 63,725,230	Y75F	probably damaging	Het
Olfr401	T	A	11: 74,121,509	D73E	probably damaging	Het
Olfr645	G	A	7: 104,084,169	R304W	probably benign	Het
Pkd1l1	A	G	11: 8,916,301	V518A	probably damaging	Het
Ptgs2	A	G	1: 150,105,528	M521V	probably benign	Het
Qsox1	T	A	1: 155,779,528	M630L	probably benign	Het
Skiv2l2	T	A	13: 112,917,662	I146F	probably damaging	Het
Spty2d1	A	G	7: 46,998,284	L299P	probably damaging	Het
St6gal1	T	G	16: 23,321,055		probably benign	Het
Sult2a6	T	C	7: 14,250,358	Y149C	probably damaging	Het
Tlr3	G	A	8: 45,402,771	T124M	probably benign	Het
Tmem171	A	G	13: 98,692,511	Y44H	probably damaging	Het
Usp35	C	T	7: 97,312,351	V623I	probably benign	Het
Vangl2	T	A	1: 172,006,127	H463L	probably damaging	Het
Vmn1r175	A	C	7: 23,808,480	L241V	probably benign	Het
Vmn1r19	A	T	6: 57,405,353	K297I	unknown	Het
Vmn2r23	C	A	6: 123,712,759	T198K	probably benign	Het
Vmn2r-ps69	T	A	7: 85,304,015		noncoding transcript	Het
Xirp2	A	G	2: 67,510,967	Y1184C	probably benign	Het
Ythdc1	A	G	5: 86,835,951		probably benign	Het
Zbtb39	C	T	10: 127,742,646	A363V	probably damaging	Het
Zmiz1	T	A	14: 25,651,304	I527K	possibly damaging	Het
Zmiz1	C	A	14: 25,651,306	P534T	probably damaging	Het

Other mutations in Gpr89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00421:Gpr89	APN	3	96898523	missense	probably damaging	1.00
IGL00757:Gpr89	APN	3	96871534	missense	probably benign	0.00
IGL01114:Gpr89	APN	3	96893549	missense	probably damaging	0.98
IGL02417:Gpr89	APN	3	96897425	nonsense	probably null
explorer	UTSW	3	96880069	splice site	probably null
R0207:Gpr89	UTSW	3	96871480	missense	probably damaging	0.99
R0650:Gpr89	UTSW	3	96897324	splice site	probably benign
R0704:Gpr89	UTSW	3	96880168	critical splice acceptor site	probably null
R1496:Gpr89	UTSW	3	96905210	missense	probably benign	0.00
R1869:Gpr89	UTSW	3	96875659	missense	probably benign	0.16
R1913:Gpr89	UTSW	3	96875633	missense	possibly damaging	0.91
R2264:Gpr89	UTSW	3	96872515	missense	probably damaging	0.99
R2276:Gpr89	UTSW	3	96897427	missense	probably damaging	1.00
R3822:Gpr89	UTSW	3	96892944	missense	probably benign	0.03
R3922:Gpr89	UTSW	3	96890899	missense	probably damaging	1.00
R4984:Gpr89	UTSW	3	96905196	missense	probably benign	0.02
R6185:Gpr89	UTSW	3	96890833	missense	probably damaging	0.99
R7063:Gpr89	UTSW	3	96875698	missense	probably damaging	1.00
R7164:Gpr89	UTSW	3	96871398	missense	probably benign	0.04
R7172:Gpr89	UTSW	3	96880069	splice site	probably null
R7215:Gpr89	UTSW	3	96880088	missense	probably damaging	1.00
R7536:Gpr89	UTSW	3	96890893	missense	probably damaging	0.96
R7708:Gpr89	UTSW	3	96880625	missense	possibly damaging	0.81
R7849:Gpr89	UTSW	3	96871490	nonsense	probably null
R7932:Gpr89	UTSW	3	96871490	nonsense	probably null
RF019:Gpr89	UTSW	3	96905193	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ATTTGGTACCCTCAGAAAGAAGG -3'
(R):5'- AAGGTTTCCTAATGGTGGTAAGAATGC -3'

Sequencing Primer
(F):5'- CCCTCAGAAAGAAGGAAACATTTTG -3'
(R):5'- CTAATGGTGGTAAGAATGCTTTTGCC -3'

Posted On

2016-11-21