Mutagenetix > Incidental Mutation

Incidental Mutation 'R5761:Gpr89'

445284

Institutional Source

Beutler Lab

Gene Symbol

Gpr89

Ensembl Gene

ENSMUSG00000028096

Gene Name

G protein-coupled receptor 89

Synonyms

SH120, 4933412D19Rik

MMRRC Submission

043363-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.383) question?

Stock #

R5761 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

96775630-96812662 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 96800196 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 134 (L134P)

Ref Sequence

ENSEMBL: ENSMUSP00000029738 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000029738] [ENSMUST00000154750]

AlphaFold

Q8BS95

Predicted Effect

probably damaging
Transcript: ENSMUST00000029738
AA Change: L134P

PolyPhen 2 Score 0.996 (Sensitivity: 0.55; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000029738
Gene: ENSMUSG00000028096
AA Change: L134P

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC
transmembrane domain	110	127	N/A	INTRINSIC
Pfam:GPHR_N	140	207	1.1e-31	PFAM
Pfam:ABA_GPCR	276	446	4.9e-46	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000139599

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000146645

Predicted Effect

probably benign
Transcript: ENSMUST00000154750

SMART Domains

Protein: ENSMUSP00000116016
Gene: ENSMUSG00000028096

Domain	Start	End	E-Value	Type
transmembrane domain	4	26	N/A	INTRINSIC
transmembrane domain	38	60	N/A	INTRINSIC
transmembrane domain	75	97	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199586

Meta Mutation Damage Score

0.6224

Coding Region Coverage

1x: 99.5%
3x: 98.8%
10x: 97.3%
20x: 95.2%

Validation Efficiency

99% (67/68)

Allele List at MGI

Other mutations in this stock

Total: 60 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca6	A	T	11: 110,100,927 (GRCm39)	V953D	probably damaging	Het
Abcb8	T	C	5: 24,610,879 (GRCm39)		probably benign	Het
Acad12	A	T	5: 121,742,243 (GRCm39)		probably benign	Het
Adam2	A	T	14: 66,283,595 (GRCm39)	C436S	probably damaging	Het
Aebp2	T	C	6: 140,569,943 (GRCm39)		probably benign	Het
Akap8	A	T	17: 32,536,159 (GRCm39)	C85S	possibly damaging	Het
Aldh1a3	C	A	7: 66,068,927 (GRCm39)	R19L	probably damaging	Het
Baz2a	A	G	10: 127,955,559 (GRCm39)	T848A	possibly damaging	Het
Bud13	C	T	9: 46,199,350 (GRCm39)	A237V	probably benign	Het
Cbarp	CGCCTCTGCTGCCTCT	CGCCTCT	10: 79,968,067 (GRCm39)		probably benign	Het
Ccdc187	G	A	2: 26,166,104 (GRCm39)	P775L	possibly damaging	Het
Ccz1	C	T	5: 143,929,328 (GRCm39)	G367R	probably damaging	Het
Cep104	T	C	4: 154,065,681 (GRCm39)	V56A	possibly damaging	Het
Chd6	C	A	2: 160,798,998 (GRCm39)	R2362S	probably damaging	Het
Chd6	C	T	2: 160,798,999 (GRCm39)	R2362K	probably damaging	Het
Cmc1	T	C	9: 117,944,443 (GRCm39)	E25G	probably benign	Het
Cntnap5b	T	C	1: 100,374,619 (GRCm39)	S1123P	probably damaging	Het
Col4a3	T	A	1: 82,693,778 (GRCm39)	L66*	probably null	Het
Crmp1	A	G	5: 37,440,212 (GRCm39)	T329A	probably benign	Het
Cybb	C	G	X: 9,316,989 (GRCm39)	D246H	probably benign	Het
Cyp3a16	A	G	5: 145,378,843 (GRCm39)	S393P	possibly damaging	Het
Cyp4f18	T	A	8: 72,749,975 (GRCm39)	I225F	probably damaging	Het
Ddhd2	A	G	8: 26,231,726 (GRCm39)	V432A	probably benign	Het
Ecpas	T	C	4: 58,853,131 (GRCm39)	I401M	probably damaging	Het
Foxq1	G	A	13: 31,743,314 (GRCm39)	A139T	probably damaging	Het
Gm10428	G	T	11: 62,644,169 (GRCm39)		probably benign	Het
Hjv	T	C	3: 96,435,938 (GRCm39)	S399P	probably benign	Het
Hrc	A	T	7: 44,986,025 (GRCm39)		probably null	Het
Igf1r	T	A	7: 67,857,001 (GRCm39)	Y988N	probably damaging	Het
Igf2r	A	C	17: 12,917,239 (GRCm39)		probably null	Het
Itga2b	A	G	11: 102,357,100 (GRCm39)	F260S	probably benign	Het
Kif2a	T	A	13: 107,098,672 (GRCm39)	N698I	probably benign	Het
Lap3	A	C	5: 45,662,147 (GRCm39)	I316L	probably benign	Het
Map2k6	T	G	11: 110,290,197 (GRCm39)		probably benign	Het
Mtrex	T	A	13: 113,054,196 (GRCm39)	I146F	probably damaging	Het
Myh1	A	G	11: 67,110,078 (GRCm39)	E1422G	probably damaging	Het
Ncoa6	A	G	2: 155,250,061 (GRCm39)	V1081A	probably benign	Het
Nom1	A	G	5: 29,642,639 (GRCm39)	E380G	probably damaging	Het
Nwd2	A	T	5: 63,882,573 (GRCm39)	Y75F	probably damaging	Het
Or3a1b	T	A	11: 74,012,335 (GRCm39)	D73E	probably damaging	Het
Or51a24	G	A	7: 103,733,376 (GRCm39)	R304W	probably benign	Het
Pkd1l1	A	G	11: 8,866,301 (GRCm39)	V518A	probably damaging	Het
Ptgs2	A	G	1: 149,981,279 (GRCm39)	M521V	probably benign	Het
Qsox1	T	A	1: 155,655,274 (GRCm39)	M630L	probably benign	Het
Spty2d1	A	G	7: 46,648,032 (GRCm39)	L299P	probably damaging	Het
St6gal1	T	G	16: 23,139,805 (GRCm39)		probably benign	Het
Sult2a6	T	C	7: 13,984,283 (GRCm39)	Y149C	probably damaging	Het
Tlr3	G	A	8: 45,855,808 (GRCm39)	T124M	probably benign	Het
Tmem171	A	G	13: 98,829,019 (GRCm39)	Y44H	probably damaging	Het
Usp35	C	T	7: 96,961,558 (GRCm39)	V623I	probably benign	Het
Vangl2	T	A	1: 171,833,694 (GRCm39)	H463L	probably damaging	Het
Vmn1r175	A	C	7: 23,507,905 (GRCm39)	L241V	probably benign	Het
Vmn1r19	A	T	6: 57,382,338 (GRCm39)	K297I	unknown	Het
Vmn2r23	C	A	6: 123,689,718 (GRCm39)	T198K	probably benign	Het
Vmn2r-ps69	T	A	7: 84,953,223 (GRCm39)		noncoding transcript	Het
Xirp2	A	G	2: 67,341,311 (GRCm39)	Y1184C	probably benign	Het
Ythdc1	A	G	5: 86,983,810 (GRCm39)		probably benign	Het
Zbtb39	C	T	10: 127,578,515 (GRCm39)	A363V	probably damaging	Het
Zmiz1	T	A	14: 25,651,728 (GRCm39)	I527K	possibly damaging	Het
Zmiz1	C	A	14: 25,651,730 (GRCm39)	P534T	probably damaging	Het

Other mutations in Gpr89

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00421:Gpr89	APN	3	96,805,839 (GRCm39)	missense	probably damaging	1.00
IGL00757:Gpr89	APN	3	96,778,850 (GRCm39)	missense	probably benign	0.00
IGL01114:Gpr89	APN	3	96,800,865 (GRCm39)	missense	probably damaging	0.98
IGL02417:Gpr89	APN	3	96,804,741 (GRCm39)	nonsense	probably null
explorer	UTSW	3	96,787,385 (GRCm39)	splice site	probably null
R0207:Gpr89	UTSW	3	96,778,796 (GRCm39)	missense	probably damaging	0.99
R0650:Gpr89	UTSW	3	96,804,640 (GRCm39)	splice site	probably benign
R0704:Gpr89	UTSW	3	96,787,484 (GRCm39)	critical splice acceptor site	probably null
R1496:Gpr89	UTSW	3	96,812,526 (GRCm39)	missense	probably benign	0.00
R1869:Gpr89	UTSW	3	96,782,975 (GRCm39)	missense	probably benign	0.16
R1913:Gpr89	UTSW	3	96,782,949 (GRCm39)	missense	possibly damaging	0.91
R2264:Gpr89	UTSW	3	96,779,831 (GRCm39)	missense	probably damaging	0.99
R2276:Gpr89	UTSW	3	96,804,743 (GRCm39)	missense	probably damaging	1.00
R3822:Gpr89	UTSW	3	96,800,260 (GRCm39)	missense	probably benign	0.03
R3922:Gpr89	UTSW	3	96,798,215 (GRCm39)	missense	probably damaging	1.00
R4984:Gpr89	UTSW	3	96,812,512 (GRCm39)	missense	probably benign	0.02
R6185:Gpr89	UTSW	3	96,798,149 (GRCm39)	missense	probably damaging	0.99
R7063:Gpr89	UTSW	3	96,783,014 (GRCm39)	missense	probably damaging	1.00
R7164:Gpr89	UTSW	3	96,778,714 (GRCm39)	missense	probably benign	0.04
R7172:Gpr89	UTSW	3	96,787,385 (GRCm39)	splice site	probably null
R7215:Gpr89	UTSW	3	96,787,404 (GRCm39)	missense	probably damaging	1.00
R7536:Gpr89	UTSW	3	96,798,209 (GRCm39)	missense	probably damaging	0.96
R7708:Gpr89	UTSW	3	96,787,941 (GRCm39)	missense	possibly damaging	0.81
R7849:Gpr89	UTSW	3	96,778,806 (GRCm39)	nonsense	probably null
R8273:Gpr89	UTSW	3	96,812,505 (GRCm39)	missense	probably benign
R9643:Gpr89	UTSW	3	96,780,813 (GRCm39)	missense	probably damaging	0.97
RF019:Gpr89	UTSW	3	96,812,509 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- ATTTGGTACCCTCAGAAAGAAGG -3'
(R):5'- AAGGTTTCCTAATGGTGGTAAGAATGC -3'

Sequencing Primer
(F):5'- CCCTCAGAAAGAAGGAAACATTTTG -3'
(R):5'- CTAATGGTGGTAAGAATGCTTTTGCC -3'

Posted On

2016-11-21