Mutagenetix > Incidental Mutation

Incidental Mutation 'PIT4131001:Col5a1'

523168

Institutional Source

Beutler Lab

Gene Symbol

Col5a1

Ensembl Gene

ENSMUSG00000026837

Gene Name

collagen, type V, alpha 1

Synonyms

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

PIT4131001 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

27776437-27929526 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 27914665 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 94 (T94A)

Ref Sequence

ENSEMBL: ENSMUSP00000123532 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028280] [ENSMUST00000145423]

AlphaFold

O88207

Predicted Effect

SMART Domains

Protein: ENSMUSP00000028280
Gene: ENSMUSG00000026837
AA Change: T1583A

Domain	Start	End	E-Value	Type
signal peptide	1	36	N/A	INTRINSIC
TSPN	39	230	5.7e-73	SMART
LamG	98	229	6.86e-3	SMART
low complexity region	259	288	N/A	INTRINSIC
low complexity region	300	314	N/A	INTRINSIC
low complexity region	335	352	N/A	INTRINSIC
low complexity region	374	387	N/A	INTRINSIC
low complexity region	412	428	N/A	INTRINSIC
internal_repeat_7	443	457	9.97e-7	PROSPERO
Pfam:Collagen	467	519	4e-10	PFAM
Pfam:Collagen	557	619	6.5e-9	PFAM
internal_repeat_2	622	642	1.83e-11	PROSPERO
low complexity region	643	698	N/A	INTRINSIC
low complexity region	712	757	N/A	INTRINSIC
low complexity region	760	793	N/A	INTRINSIC
internal_repeat_5	794	817	3.78e-8	PROSPERO
internal_repeat_7	798	812	9.97e-7	PROSPERO
internal_repeat_8	802	821	8.84e-6	PROSPERO
low complexity region	826	862	N/A	INTRINSIC
internal_repeat_3	865	889	2.79e-10	PROSPERO
internal_repeat_5	869	892	3.78e-8	PROSPERO
low complexity region	895	925	N/A	INTRINSIC
internal_repeat_2	928	948	1.83e-11	PROSPERO
internal_repeat_4	928	948	1.27e-8	PROSPERO
low complexity region	949	979	N/A	INTRINSIC
low complexity region	984	1033	N/A	INTRINSIC
internal_repeat_4	1039	1062	1.27e-8	PROSPERO
internal_repeat_1	1039	1063	5.12e-15	PROSPERO
internal_repeat_3	1048	1072	2.79e-10	PROSPERO
internal_repeat_6	1049	1072	1.13e-7	PROSPERO
low complexity region	1075	1117	N/A	INTRINSIC
low complexity region	1134	1165	N/A	INTRINSIC
low complexity region	1174	1193	N/A	INTRINSIC
internal_repeat_8	1195	1214	8.84e-6	PROSPERO
low complexity region	1215	1243	N/A	INTRINSIC
low complexity region	1249	1282	N/A	INTRINSIC
low complexity region	1285	1421	N/A	INTRINSIC
internal_repeat_1	1423	1447	5.12e-15	PROSPERO
Pfam:Collagen	1460	1529	8.4e-9	PFAM
Pfam:Collagen	1513	1575	1.2e-9	PFAM
COLFI	1608	1837	3.33e-153	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000123129

Predicted Effect

probably benign
Transcript: ENSMUST00000145423
AA Change: T94A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000123532
Gene: ENSMUSG00000026837
AA Change: T94A

Domain	Start	End	E-Value	Type
Pfam:Collagen	1	50	2.7e-10	PFAM
COLFI	119	348	3.33e-153	SMART

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

92% (126/137)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an alpha chain for one of the low abundance fibrillar collagens. Fibrillar collagen molecules are trimers that can be composed of one or more types of alpha chains. Type V collagen is found in tissues containing type I collagen and appears to regulate the assembly of heterotypic fibers composed of both type I and type V collagen. This gene product is closely related to type XI collagen and it is possible that the collagen chains of types V and XI constitute a single collagen type with tissue-specific chain combinations. The encoded procollagen protein occurs commonly as the heterotrimer pro-alpha1(V)-pro-alpha1(V)-pro-alpha2(V). Mutations in this gene are associated with Ehlers-Danlos syndrome, types I and II. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2013]
PHENOTYPE: Homozygous mutation of this gene results in lethality around E10-11 due to cardiovascular insufficiency and lack of collagen fibril formation. Heterozygotes exhibit poorly organized and less dense fibers in the dermis and reduced skin tensile strength and are a model for Ehlers-Danlos Syndrome. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 129 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700010B08Rik	C	T	2: 173,561,599 (GRCm39)		probably benign	Het
Alpk2	G	A	18: 65,439,450 (GRCm39)	H648Y	possibly damaging	Het
Ambp	A	G	4: 63,062,502 (GRCm39)	Y246H	probably damaging	Het
Amz1	T	C	5: 140,735,088 (GRCm39)		probably null	Het
Anks6	G	A	4: 47,027,109 (GRCm39)	T703I	probably damaging	Het
Armc2	A	G	10: 41,823,883 (GRCm39)		probably benign	Het
Atp7b	T	A	8: 22,484,672 (GRCm39)	I1347F	probably damaging	Het
Atp8a1	C	T	5: 67,779,945 (GRCm39)	W1149*	probably null	Het
Auh	A	G	13: 52,995,046 (GRCm39)	I173T	probably damaging	Het
Axin2	T	C	11: 108,814,829 (GRCm39)	L239P	possibly damaging	Het
Bbs1	A	T	19: 4,949,287 (GRCm39)	F257L	possibly damaging	Het
Cacna2d2	C	T	9: 107,401,867 (GRCm39)	P774L	probably damaging	Het
Card6	A	G	15: 5,137,788 (GRCm39)	L22P	probably damaging	Het
Ccdc171	C	T	4: 83,579,946 (GRCm39)			Het
Ccn2	T	C	10: 24,471,988 (GRCm39)	V70A	probably damaging	Het
Cdc14a	T	A	3: 116,122,310 (GRCm39)	N219I	possibly damaging	Het
Cfap65	G	T	1: 74,967,501 (GRCm39)	N192K	probably benign	Het
Col14a1	T	C	15: 55,312,272 (GRCm39)		probably benign	Het
Col6a5	T	C	9: 105,759,113 (GRCm39)	N2031S	probably damaging	Het
Col7a1	T	C	9: 108,794,989 (GRCm39)		probably benign	Het
Cyld	T	C	8: 89,473,543 (GRCm39)	S739P	probably damaging	Het
Dbr1	A	G	9: 99,466,072 (GRCm39)		probably null	Het
Dip2b	T	C	15: 100,100,233 (GRCm39)	L1267P	probably damaging	Het
Dolk	A	G	2: 30,175,586 (GRCm39)	M153T	probably benign	Het
Duxf1	C	T	10: 58,060,704 (GRCm39)	E17K	possibly damaging	Het
Duxf1	G	A	10: 58,060,136 (GRCm39)		probably benign	Het
Duxf1	A	G	10: 58,059,276 (GRCm39)	C493R	probably benign	Het
Duxf3	A	C	10: 58,067,498 (GRCm39)	S27A	probably benign	Het
Eef1d	C	T	15: 75,775,581 (GRCm39)	R26H	probably benign	Homo
Efcab5	C	T	11: 77,028,517 (GRCm39)			Het
Epc1	T	C	18: 6,449,246 (GRCm39)	D467G	probably damaging	Het
Fancm	T	G	12: 65,152,196 (GRCm39)	M884R	probably benign	Het
Fbxo24	G	T	5: 137,620,164 (GRCm39)	H15N	probably damaging	Het
Frem1	A	G	4: 82,924,045 (GRCm39)	F305L	probably damaging	Het
Fstl5	A	G	3: 76,567,006 (GRCm39)	D550G	probably damaging	Het
Gcnt3	T	G	9: 69,941,326 (GRCm39)	K414T	possibly damaging	Het
Gm10718	A	T	9: 3,024,417 (GRCm39)	T134S	probably benign	Het
Gm10722	A	G,C	9: 3,001,414 (GRCm39)		probably benign	Het
Gm10800	T	C	2: 98,497,163 (GRCm39)	R152G	probably benign	Homo
Gm10800	C	A	2: 98,497,250 (GRCm39)	V123F	probably benign	Homo
Gm10800	A	C	2: 98,496,893 (GRCm39)	F220C	probably benign	Het
Gm10801	A	G	2: 98,492,648 (GRCm39)	R23G	probably benign	Homo
Gm11168	C	T	9: 3,004,605 (GRCm39)	P49S	probably benign	Het
Gm21738	G	A	14: 19,417,330 (GRCm38)	S66L	probably benign	Het
Hjurp	TCTGGGAGGGCTTGCTCCGGGGGCAGTGTGTCCTGTTCTTGTGCAGCCCCTG	T	1: 88,194,000 (GRCm39)		probably benign	Het
Hmgcr	A	G	13: 96,795,562 (GRCm39)	Y336H	probably damaging	Het
Hoxa13	G	C	6: 52,260,648 (GRCm38)		probably benign	Homo
Hoxa13	C	G	6: 52,260,647 (GRCm38)		probably benign	Homo
Igf2bp3	A	C	6: 49,094,084 (GRCm39)		probably null	Het
Kcnb2	A	T	1: 15,383,200 (GRCm39)	K175N	possibly damaging	Het
Kdr	T	C	5: 76,102,631 (GRCm39)		probably benign	Het
Kif5c	A	G	2: 49,584,044 (GRCm39)	K160E	probably damaging	Het
Kif7	A	T	7: 79,360,817 (GRCm39)	V186E	probably damaging	Het
Krt16	T	A	11: 100,139,575 (GRCm39)	T48S	unknown	Het
Liph	T	C	16: 21,814,119 (GRCm39)	M1V	probably null	Het
Mctp2	A	G	7: 71,740,005 (GRCm39)	F795S	probably damaging	Het
Muc4	T	A	16: 32,755,699 (GRCm38)		probably benign	Homo
Muc4	C	G	16: 32,755,676 (GRCm38)		probably benign	Homo
Muc4	T	A	16: 32,755,684 (GRCm38)		probably benign	Homo
Myo15a	G	T	11: 60,373,953 (GRCm39)	A1267S	probably damaging	Het
Myo15a	T	C	11: 60,386,280 (GRCm39)	Y1802H	probably damaging	Het
Myo7b	G	A	18: 32,094,259 (GRCm39)	T1963I	probably benign	Het
Nadk2	TG	T	15: 9,100,232 (GRCm39)		probably null	Homo
Naip5	T	C	13: 100,356,268 (GRCm39)	N1116D	probably benign	Het
Naip5	T	C	13: 100,356,247 (GRCm39)	R1123G	probably benign	Het
Nap1l1	A	G	10: 111,322,583 (GRCm39)	D61G	probably null	Het
Napsa	A	T	7: 44,230,875 (GRCm39)	T81S	probably damaging	Het
Ngp	T	C	9: 110,251,337 (GRCm39)		probably benign	Het
Nktr	T	A	9: 121,570,687 (GRCm39)	V143E	probably damaging	Het
Obscn	A	G	11: 58,957,890 (GRCm39)		probably null	Het
Or10ag53	G	A	2: 87,082,973 (GRCm39)	A231T	probably benign	Het
Or11i1	T	C	3: 106,729,282 (GRCm39)	I198V	probably benign	Het
Or4f56	G	A	2: 111,703,649 (GRCm39)	L184F	probably benign	Het
Or52ab2	C	T	7: 102,970,076 (GRCm39)	R153*	probably null	Het
Or52d3	G	A	7: 104,229,237 (GRCm39)	R128Q	probably damaging	Het
Or6p1	A	G	1: 174,258,390 (GRCm39)	Y132C	probably damaging	Het
Paip2b	A	G	6: 83,785,823 (GRCm39)	Y136H	probably damaging	Het
Pde2a	G	T	7: 101,160,361 (GRCm39)	R845L	probably damaging	Het
Pgap2	A	G	7: 101,886,405 (GRCm39)	Y197C	possibly damaging	Het
Phf11b	A	G	14: 59,560,611 (GRCm39)		probably benign	Het
Pitpnm2	A	T	5: 124,269,178 (GRCm39)	D481E	probably benign	Het
Pxk	A	T	14: 8,152,130 (GRCm38)	H482L	probably benign	Het
Rad50	A	G	11: 53,585,726 (GRCm39)		probably null	Het
Rbmyf1	T	A	Y: 2,787,132 (GRCm39)	N228Y	probably benign	Het
Rbmyf5	T	C	Y: 3,297,411 (GRCm39)	H235R	probably benign	Het
Rbmyf6	C	T	Y: 3,328,944 (GRCm39)	A241T	possibly damaging	Het
Rnf220	A	G	4: 117,134,566 (GRCm39)		probably null	Het
Saxo5	T	A	8: 3,526,062 (GRCm39)	S72T	possibly damaging	Het
Selenbp1	C	T	3: 94,844,607 (GRCm39)	T88M	probably damaging	Het
Sft2d1	T	C	17: 8,609,863 (GRCm39)	I104T	possibly damaging	Het
Sik1	A	G	17: 32,070,305 (GRCm39)	S135P	probably damaging	Het
Slc16a3	T	C	11: 120,846,172 (GRCm39)	F34L	probably damaging	Het
Slc6a20b	T	C	9: 123,612,126 (GRCm38)	N85S	probably benign	Homo
Snrnp27	T	C	6: 86,659,893 (GRCm39)	R34G	unknown	Het
Sos2	T	C	12: 69,664,851 (GRCm39)	H393R	probably benign	Het
Sp110	C	T	1: 85,513,971 (GRCm39)	R262Q	probably benign	Het
Sp110	T	C	1: 85,513,975 (GRCm39)	R261G	probably benign	Het
Sp140	T	A	1: 85,528,893 (GRCm39)	Y5N	probably benign	Het
Sp140	A	G	1: 85,570,942 (GRCm39)	S461G	probably benign	Het
Sp140	G	C	1: 85,538,603 (GRCm39)	K113N	probably benign	Het
Sp140l1	G	A	1: 85,077,341 (GRCm39)	A75V	probably benign	Het
Sp140l2	A	C	1: 85,223,395 (GRCm39)		probably benign	Het
Speer4a2	A	T	5: 26,291,485 (GRCm39)	F107Y	probably benign	Het
Speer4a2	C	G	5: 26,294,093 (GRCm39)	W28C	probably damaging	Het
Ssrp1	G	A	2: 84,868,760 (GRCm39)	V40M	probably damaging	Het
Tada2a	T	C	11: 83,970,563 (GRCm39)	E202G	probably damaging	Het
Tcf20	C	A	15: 82,735,785 (GRCm39)	A1889S	probably damaging	Het
Tdrd12	A	T	7: 35,180,528 (GRCm39)	Y828*	probably null	Het
Tlr2	T	A	3: 83,745,756 (GRCm39)	D109V	probably benign	Het
Tomm40	G	A	7: 19,437,016 (GRCm39)	T17M	probably damaging	Het
Tsga10ip	T	C	19: 5,440,161 (GRCm39)	T135A	possibly damaging	Het
Tspan8	G	A	10: 115,653,515 (GRCm39)	V4M	probably damaging	Het
Ttc28	A	T	5: 111,040,719 (GRCm39)	T36S	probably benign	Het
Ugt1a6b	G	A	1: 88,146,112 (GRCm39)	R519Q	probably damaging	Het
Ugt1a6b	TTCA	T	1: 88,143,880 (GRCm39)		probably benign	Het
Ugt1a6b	G	A	1: 88,143,976 (GRCm39)	A199T	probably damaging	Het
Unc45a	A	G	7: 79,976,109 (GRCm39)	M790T	possibly damaging	Het
Vav3	T	C	3: 109,571,751 (GRCm39)		probably null	Het
Vcpkmt	G	A	12: 69,629,552 (GRCm39)	S70L	probably benign	Het
Vmn1r3	C	T	4: 3,184,691 (GRCm39)	M205I	probably damaging	Het
Vmn1r3	C	T	4: 3,184,774 (GRCm39)	V178I	probably benign	Het
Vmn2r66	T	C	7: 84,644,301 (GRCm39)	Q703R	probably damaging	Het
Vmn2r98	G	T	17: 19,301,223 (GRCm39)	V742F	probably benign	Het
Wfdc8	A	G	2: 164,439,696 (GRCm39)	S229P	possibly damaging	Het
Xpo4	A	T	14: 57,822,068 (GRCm39)	C1083S	probably null	Het
Zbtb38	T	C	9: 96,568,369 (GRCm39)	D905G	probably damaging	Het
Zbtb8b	A	G	4: 129,321,308 (GRCm39)	*518Q	probably null	Het
Zfp600	TC	T	4: 146,131,802 (GRCm39)		probably null	Het
Zfp992	C	T	4: 146,550,569 (GRCm39)	P97S	probably benign	Het

Other mutations in Col5a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01291:Col5a1	APN	2	27,861,456 (GRCm39)	splice site	probably benign
IGL01340:Col5a1	APN	2	27,850,463 (GRCm39)	missense	unknown
IGL01938:Col5a1	APN	2	27,886,885 (GRCm39)	missense	unknown
IGL02167:Col5a1	APN	2	27,908,568 (GRCm39)	missense	probably benign
IGL02670:Col5a1	APN	2	27,864,727 (GRCm39)	missense	unknown
IGL02672:Col5a1	APN	2	27,864,727 (GRCm39)	missense	unknown
IGL02673:Col5a1	APN	2	27,864,727 (GRCm39)	missense	unknown
IGL02832:Col5a1	APN	2	27,842,352 (GRCm39)	missense	unknown
IGL03065:Col5a1	APN	2	27,922,757 (GRCm39)	missense	possibly damaging	0.61
IGL03196:Col5a1	APN	2	27,865,610 (GRCm39)	missense	unknown
PIT4495001:Col5a1	UTSW	2	27,914,788 (GRCm39)	missense	unknown
R0136:Col5a1	UTSW	2	27,914,843 (GRCm39)	missense	probably damaging	1.00
R0485:Col5a1	UTSW	2	27,880,109 (GRCm39)	splice site	probably benign
R0626:Col5a1	UTSW	2	27,818,255 (GRCm39)	nonsense	probably null
R0666:Col5a1	UTSW	2	27,922,697 (GRCm39)	missense	probably damaging	1.00
R1268:Col5a1	UTSW	2	27,892,501 (GRCm39)	missense	unknown
R1302:Col5a1	UTSW	2	27,895,248 (GRCm39)	missense	probably damaging	1.00
R1416:Col5a1	UTSW	2	27,812,076 (GRCm39)	missense	unknown
R1466:Col5a1	UTSW	2	27,893,858 (GRCm39)	splice site	probably benign
R1617:Col5a1	UTSW	2	27,842,393 (GRCm39)	missense	unknown
R1650:Col5a1	UTSW	2	27,812,171 (GRCm39)	missense	unknown
R1663:Col5a1	UTSW	2	27,841,488 (GRCm39)	missense	unknown
R1901:Col5a1	UTSW	2	27,850,456 (GRCm39)	missense	unknown
R1970:Col5a1	UTSW	2	27,876,766 (GRCm39)	missense	unknown
R2377:Col5a1	UTSW	2	27,818,189 (GRCm39)	missense	unknown
R2396:Col5a1	UTSW	2	27,876,741 (GRCm39)	missense	unknown
R4297:Col5a1	UTSW	2	27,907,216 (GRCm39)	critical splice donor site	probably null
R4385:Col5a1	UTSW	2	27,914,791 (GRCm39)	missense	probably damaging	1.00
R4803:Col5a1	UTSW	2	27,901,353 (GRCm39)	missense	unknown
R4835:Col5a1	UTSW	2	27,915,656 (GRCm39)	missense	probably damaging	1.00
R4935:Col5a1	UTSW	2	27,914,754 (GRCm39)	missense	probably damaging	1.00
R4994:Col5a1	UTSW	2	27,922,751 (GRCm39)	missense	possibly damaging	0.90
R4997:Col5a1	UTSW	2	27,922,794 (GRCm39)	nonsense	probably null
R5061:Col5a1	UTSW	2	27,842,390 (GRCm39)	missense	unknown
R5088:Col5a1	UTSW	2	27,908,614 (GRCm39)	nonsense	probably null
R5089:Col5a1	UTSW	2	27,908,614 (GRCm39)	nonsense	probably null
R5090:Col5a1	UTSW	2	27,908,614 (GRCm39)	nonsense	probably null
R5114:Col5a1	UTSW	2	27,915,664 (GRCm39)	missense	probably damaging	1.00
R5409:Col5a1	UTSW	2	27,850,457 (GRCm39)	missense	unknown
R5649:Col5a1	UTSW	2	27,841,468 (GRCm39)	missense	unknown
R5699:Col5a1	UTSW	2	27,887,611 (GRCm39)	missense	unknown
R5910:Col5a1	UTSW	2	27,926,900 (GRCm39)	missense	possibly damaging	0.89
R6053:Col5a1	UTSW	2	27,904,389 (GRCm39)	unclassified	probably benign
R6210:Col5a1	UTSW	2	27,922,633 (GRCm39)	missense	probably benign	0.04
R6363:Col5a1	UTSW	2	27,818,207 (GRCm39)	missense	unknown
R6478:Col5a1	UTSW	2	27,842,448 (GRCm39)	missense	unknown
R6600:Col5a1	UTSW	2	27,887,583 (GRCm39)	missense	unknown
R7047:Col5a1	UTSW	2	27,818,096 (GRCm39)	missense	unknown
R7061:Col5a1	UTSW	2	27,915,690 (GRCm39)	nonsense	probably null
R7131:Col5a1	UTSW	2	27,819,498 (GRCm39)	missense	unknown
R7202:Col5a1	UTSW	2	27,842,390 (GRCm39)	missense	unknown
R7270:Col5a1	UTSW	2	27,887,597 (GRCm39)	missense	unknown
R7385:Col5a1	UTSW	2	27,914,762 (GRCm39)	missense	unknown
R7492:Col5a1	UTSW	2	27,859,812 (GRCm39)	critical splice donor site	probably null
R7570:Col5a1	UTSW	2	27,841,395 (GRCm39)	missense	unknown
R7627:Col5a1	UTSW	2	27,840,665 (GRCm39)	nonsense	probably null
R8003:Col5a1	UTSW	2	27,848,340 (GRCm39)	intron	probably benign
R8011:Col5a1	UTSW	2	27,870,533 (GRCm39)	splice site	probably benign
R8073:Col5a1	UTSW	2	27,852,141 (GRCm39)	missense	possibly damaging	0.85
R8217:Col5a1	UTSW	2	27,812,135 (GRCm39)	missense	unknown
R8879:Col5a1	UTSW	2	27,904,170 (GRCm39)	missense	unknown
R8911:Col5a1	UTSW	2	27,887,630 (GRCm39)	critical splice donor site	probably null
R9082:Col5a1	UTSW	2	27,852,122 (GRCm39)	missense	possibly damaging	0.73
R9095:Col5a1	UTSW	2	27,914,665 (GRCm39)	missense	probably benign	0.01
R9170:Col5a1	UTSW	2	27,841,363 (GRCm39)	missense	unknown
R9264:Col5a1	UTSW	2	27,854,123 (GRCm39)	missense	unknown
R9265:Col5a1	UTSW	2	27,854,123 (GRCm39)	missense	unknown
R9461:Col5a1	UTSW	2	27,922,616 (GRCm39)	missense	unknown
R9596:Col5a1	UTSW	2	27,819,551 (GRCm39)	nonsense	probably null
R9614:Col5a1	UTSW	2	27,879,186 (GRCm39)	missense	unknown
R9691:Col5a1	UTSW	2	27,842,994 (GRCm39)	missense	unknown
R9743:Col5a1	UTSW	2	27,864,505 (GRCm39)	missense	unknown
Z1176:Col5a1	UTSW	2	27,892,529 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TGTATGTGCATGCAGGAAAAGC -3'
(R):5'- TAGATCCTTGCAGGTACGGG -3'

Sequencing Primer
(F):5'- TGCTGCCAGTCCCTAGAC -3'
(R):5'- CAGGTACGGGCTGGGTTC -3'

Posted On

2018-06-12