Mutagenetix > Incidental Mutation

Incidental Mutation 'R6612:Itgb4'

523698

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

MMRRC Submission

044735-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R6612 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

115974709-116008412 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 115984071 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Valine at position 418 (D418V)

Ref Sequence

ENSEMBL: ENSMUSP00000102069 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

AlphaFold

A2A863

Predicted Effect

probably benign
Transcript: ENSMUST00000021107
AA Change: D418V

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: D418V

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000068981
AA Change: D418V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: D418V

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106458
AA Change: D418V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: D418V

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106460
AA Change: D418V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: D418V

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106461
AA Change: D418V

PolyPhen 2 Score 0.002 (Sensitivity: 0.99; Specificity: 0.30)

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: D418V

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151691

Predicted Effect

probably benign
Transcript: ENSMUST00000169928
AA Change: D418V

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: D418V

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180782

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.0%
20x: 94.4%

Validation Efficiency

95% (59/62)

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 61 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310035C23Rik	T	A	1: 105,692,007 (GRCm38)	D320E	possibly damaging	Het
4930523C07Rik	A	C	1: 160,075,234 (GRCm38)	N25H	probably damaging	Het
Akr1c14	A	T	13: 4,065,331 (GRCm38)	S87C	probably benign	Het
Arhgef37	T	C	18: 61,494,881 (GRCm38)	T664A	probably benign	Het
Arsi	A	G	18: 60,912,456 (GRCm38)	T73A	probably benign	Het
Cacnb2	A	T	2: 14,975,149 (GRCm38)	T274S	probably benign	Het
Cd244	A	G	1: 171,574,104 (GRCm38)	T133A	probably benign	Het
Ciz1	T	A	2: 32,377,311 (GRCm38)	S720T	possibly damaging	Het
Cxcr6	T	A	9: 123,810,720 (GRCm38)	I262N	probably damaging	Het
Cyp2a4	T	A	7: 26,308,647 (GRCm38)	F160I	probably benign	Het
Esrra	T	C	19: 6,911,852 (GRCm38)	T390A	probably benign	Het
Fopnl	TTGTG	TTG	16: 14,300,145 (GRCm38)		probably null	Het
Gm17079	C	A	14: 51,694,375 (GRCm38)	Q91H	probably damaging	Het
Gm17079	T	A	14: 51,694,376 (GRCm38)	Q91L	possibly damaging	Het
Got1	A	G	19: 43,504,803 (GRCm38)	S256P	probably damaging	Het
Gria4	C	T	9: 4,472,206 (GRCm38)	V428I	possibly damaging	Het
Grin2b	A	G	6: 135,740,998 (GRCm38)	Y699H	probably damaging	Het
Hipk2	T	C	6: 38,818,873 (GRCm38)	I154V	probably benign	Het
Hkdc1	T	C	10: 62,395,441 (GRCm38)	E628G	possibly damaging	Het
Hmcn1	C	G	1: 150,595,118 (GRCm38)		probably null	Het
Hspbap1	T	G	16: 35,801,591 (GRCm38)	L102W	probably damaging	Het
Iqcb1	G	A	16: 36,871,661 (GRCm38)		probably benign	Het
Itga7	A	G	10: 128,948,993 (GRCm38)	Y763C	possibly damaging	Het
Jakmip2	T	C	18: 43,557,367 (GRCm38)	D631G	probably damaging	Het
Kcnc2	G	C	10: 112,271,856 (GRCm38)	G51R	probably benign	Het
Kcnu1	A	G	8: 25,918,316 (GRCm38)	I52V	probably benign	Het
Kdm5a	T	C	6: 120,430,228 (GRCm38)	I1468T	probably damaging	Het
Kmt2d	A	G	15: 98,845,858 (GRCm38)		probably benign	Het
Mab21l3	A	G	3: 101,818,645 (GRCm38)	V345A	possibly damaging	Het
March10	A	T	11: 105,397,078 (GRCm38)	S133T	probably damaging	Het
Mccc1	T	C	3: 35,993,930 (GRCm38)	S115G	probably benign	Het
Mchr1	G	A	15: 81,237,870 (GRCm38)	V274M	probably damaging	Het
Mrgpra3	T	A	7: 47,590,035 (GRCm38)	I48F	probably benign	Het
Myo9a	T	A	9: 59,827,196 (GRCm38)	F687Y	probably damaging	Het
Nfrkb	T	A	9: 31,397,006 (GRCm38)	L216*	probably null	Het
Nrxn3	A	T	12: 89,813,332 (GRCm38)		probably benign	Het
Olfr746	A	T	14: 50,653,633 (GRCm38)	Y132F	probably damaging	Het
Olig2	T	A	16: 91,226,881 (GRCm38)	M161K	probably damaging	Het
Pcdh15	T	A	10: 74,185,378 (GRCm38)	N141K	probably damaging	Het
Pcdha4	T	C	18: 36,954,978 (GRCm38)	V738A	probably benign	Het
Pdgfra	T	C	5: 75,167,842 (GRCm38)	S212P	probably benign	Het
Plk3	G	A	4: 117,132,737 (GRCm38)	Q194*	probably null	Het
Ppp1r36	T	C	12: 76,437,604 (GRCm38)	I216T	possibly damaging	Het
Ptprz1	T	A	6: 23,052,082 (GRCm38)	N2303K	probably damaging	Het
Rab25	G	A	3: 88,543,403 (GRCm38)	T117M	probably damaging	Het
Slc25a47	T	C	12: 108,855,978 (GRCm38)	V231A	probably benign	Het
Slx4	G	A	16: 3,985,276 (GRCm38)	H1225Y	probably damaging	Het
Snx13	C	T	12: 35,106,759 (GRCm38)	A470V	probably benign	Het
Spa17	A	G	9: 37,605,794 (GRCm38)	F101S	probably benign	Het
Ssh1	C	T	5: 113,958,730 (GRCm38)	A217T	probably benign	Het
Synm	G	C	7: 67,733,516 (GRCm38)	T1466S	probably damaging	Het
Tbc1d19	A	G	5: 53,809,845 (GRCm38)	E29G	possibly damaging	Het
Teddm2	T	A	1: 153,850,445 (GRCm38)	T175S	probably benign	Het
Tet2	T	A	3: 133,487,335 (GRCm38)	H446L	possibly damaging	Het
Tmem110	T	A	14: 30,871,564 (GRCm38)		probably null	Het
Tpm3-rs7	G	T	14: 113,314,836 (GRCm38)	R54L	probably benign	Het
Ttc5	T	A	14: 50,785,469 (GRCm38)		probably null	Het
Tyk2	G	T	9: 21,108,016 (GRCm38)	Q1014K	probably benign	Het
Ush2a	T	C	1: 188,911,397 (GRCm38)	S4319P	possibly damaging	Het
Zbtb10	C	A	3: 9,252,065 (GRCm38)	H312Q	possibly damaging	Het
Zfp462	A	T	4: 55,012,324 (GRCm38)		probably null	Het

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115,990,940 (GRCm38)	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115,990,920 (GRCm38)	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	116,006,457 (GRCm38)	splice site	probably benign
IGL01750:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	116,003,387 (GRCm38)	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115,988,926 (GRCm38)	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	116,007,969 (GRCm38)	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	116,005,966 (GRCm38)	missense	probably benign
IGL02879:Itgb4	APN	11	115,994,352 (GRCm38)	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115,988,905 (GRCm38)	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115,988,724 (GRCm38)	missense	probably damaging	1.00
IGL03054:Itgb4	UTSW	11	116,000,340 (GRCm38)	nonsense	probably null
R0021:Itgb4	UTSW	11	115,979,627 (GRCm38)	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115,979,124 (GRCm38)	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115,979,412 (GRCm38)	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	116,007,602 (GRCm38)	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115,979,756 (GRCm38)	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115,979,695 (GRCm38)	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115,991,009 (GRCm38)	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115,979,768 (GRCm38)	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115,993,342 (GRCm38)	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115,998,162 (GRCm38)	intron	probably benign
R1381:Itgb4	UTSW	11	115,994,337 (GRCm38)	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115,990,884 (GRCm38)	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115,979,111 (GRCm38)	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115,984,164 (GRCm38)	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115,984,047 (GRCm38)	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115,999,799 (GRCm38)	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115,980,991 (GRCm38)	missense	probably damaging	1.00
R1623:Itgb4	UTSW	11	115,991,316 (GRCm38)	nonsense	probably null
R1633:Itgb4	UTSW	11	116,007,760 (GRCm38)	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	116,007,357 (GRCm38)	missense	probably damaging	1.00
R1669:Itgb4	UTSW	11	115,991,330 (GRCm38)	missense	probably benign	0.07
R1713:Itgb4	UTSW	11	116,003,489 (GRCm38)	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115,988,918 (GRCm38)	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115,988,520 (GRCm38)	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115,983,764 (GRCm38)	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115,980,738 (GRCm38)	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115,993,453 (GRCm38)	nonsense	probably null
R2102:Itgb4	UTSW	11	116,005,735 (GRCm38)	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115,979,624 (GRCm38)	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115,993,435 (GRCm38)	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	116,006,563 (GRCm38)	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	116,003,670 (GRCm38)	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	116,005,926 (GRCm38)	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	116,006,470 (GRCm38)	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115,990,935 (GRCm38)	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115,988,729 (GRCm38)	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115,999,814 (GRCm38)	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115,983,261 (GRCm38)	splice site	silent
R4585:Itgb4	UTSW	11	115,993,325 (GRCm38)	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115,993,325 (GRCm38)	missense	probably damaging	1.00
R4601:Itgb4	UTSW	11	116,005,722 (GRCm38)	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	116,006,605 (GRCm38)	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115,988,591 (GRCm38)	intron	probably benign
R5084:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	116,006,539 (GRCm38)	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115,989,749 (GRCm38)	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115,985,068 (GRCm38)	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115,988,432 (GRCm38)	intron	probably benign
R5812:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115,990,922 (GRCm38)	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115,990,922 (GRCm38)	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115,990,922 (GRCm38)	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115,979,386 (GRCm38)	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115,994,276 (GRCm38)	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	116,000,411 (GRCm38)	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115,998,137 (GRCm38)	missense	possibly damaging	0.83
R6258:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R6259:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115,984,157 (GRCm38)	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	116,005,565 (GRCm38)	nonsense	probably null
R7371:Itgb4	UTSW	11	115,998,080 (GRCm38)	missense	probably benign	0.29
R7605:Itgb4	UTSW	11	116,006,476 (GRCm38)	missense	probably benign	0.01
R7659:Itgb4	UTSW	11	115,979,731 (GRCm38)	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	116,003,710 (GRCm38)	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	116,003,684 (GRCm38)	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	116,000,261 (GRCm38)	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115,982,705 (GRCm38)	missense	probably benign	0.18
R7923:Itgb4	UTSW	11	115,982,699 (GRCm38)	critical splice acceptor site	probably null
R8004:Itgb4	UTSW	11	115,982,705 (GRCm38)	missense	probably benign	0.00
R8143:Itgb4	UTSW	11	115,993,429 (GRCm38)	missense	probably damaging	1.00
R8427:Itgb4	UTSW	11	115,991,718 (GRCm38)	critical splice donor site	probably null
R8857:Itgb4	UTSW	11	115,981,027 (GRCm38)	missense	probably benign	0.04
R8863:Itgb4	UTSW	11	115,985,072 (GRCm38)	nonsense	probably null
R8932:Itgb4	UTSW	11	115,988,469 (GRCm38)	missense	probably benign	0.01
R9153:Itgb4	UTSW	11	115,984,053 (GRCm38)	missense	probably benign	0.00
R9207:Itgb4	UTSW	11	116,007,097 (GRCm38)	missense	probably damaging	1.00
R9239:Itgb4	UTSW	11	116,007,304 (GRCm38)	missense	probably damaging	1.00
R9267:Itgb4	UTSW	11	115,979,639 (GRCm38)	missense	probably benign
R9289:Itgb4	UTSW	11	115,994,361 (GRCm38)	missense	probably benign	0.01
R9328:Itgb4	UTSW	11	115,989,799 (GRCm38)	missense	probably benign	0.00
R9435:Itgb4	UTSW	11	116,005,029 (GRCm38)	missense	probably benign	0.01
R9450:Itgb4	UTSW	11	115,983,271 (GRCm38)	missense	probably damaging	1.00
R9649:Itgb4	UTSW	11	115,994,345 (GRCm38)	missense	possibly damaging	0.78
R9779:Itgb4	UTSW	11	115,991,659 (GRCm38)	missense	probably damaging	1.00
X0062:Itgb4	UTSW	11	115,993,452 (GRCm38)	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	116,006,520 (GRCm38)	missense	probably damaging	1.00
Z1177:Itgb4	UTSW	11	115,998,058 (GRCm38)	missense	probably benign	0.00
Z1177:Itgb4	UTSW	11	115,986,811 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TGATCTCTTGGAGGAGCCTG -3'
(R):5'- TCTCTGTGACTTGGGACAGC -3'

Sequencing Primer
(F):5'- AGGAGCCTGGCACTTTCCTC -3'
(R):5'- TTTACCCAGGGTCCTAGTGCAG -3'

Posted On

2018-06-22