|Institutional Source||Beutler Lab|
|Gene Name||DGCR8, microprocessor complex subunit|
|Synonyms||Gy1, D16H22S788E, D16Wis2, D16H22S1742E, Vo59c07, N41|
|Is this an essential gene?||Essential (E-score: 1.000)|
|Stock #||R6786 (G1)|
|Chromosomal Location||18253948-18289246 bp(-) (GRCm38)|
|Type of Mutation||nonsense|
|DNA Base Change (assembly)||A to T at 18283829 bp|
|Amino Acid Change||Tyrosine to Stop codon at position 196 (Y196*)|
|Ref Sequence||ENSEMBL: ENSMUSP00000009321 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000009321] [ENSMUST00000232424]|
|Predicted Effect||probably null
AA Change: Y196*
AA Change: Y196*
|Predicted Effect||noncoding transcript
|Coding Region Coverage||
|Validation Efficiency||96% (66/69)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]
PHENOTYPE: Mice heterozygous for a gene trap allele exhibit reduced dendritic spine number and dendritiic complexity along with abnormal prepulse inhibition and abnormal spatial working memory. Homozygous mice are embryonic lethal. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dgcr8||
(F):5'- TTGGGTTTTGCTCAGAGCCC -3'
(R):5'- TGAGCTTTACTGAGAGCTGC -3'
(F):5'- TTTGCTCAGAGCCCTCCAGG -3'
(R):5'- CTTTACTGAGAGCTGCAGGAG -3'