Mutagenetix > Incidental Mutation

Incidental Mutation 'R7003:Zfp658'

544664

Institutional Source

Beutler Lab

Gene Symbol

Zfp658

Ensembl Gene

ENSMUSG00000056592

Gene Name

zinc finger protein 658

Synonyms

MMRRC Submission

045108-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7003 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

43211680-43224885 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 43224172 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Glutamic Acid at position 816 (K816E)

Ref Sequence

ENSEMBL: ENSMUSP00000103606 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000005597] [ENSMUST00000058104] [ENSMUST00000107972] [ENSMUST00000205769]

AlphaFold

Q5PPQ4

Predicted Effect

possibly damaging
Transcript: ENSMUST00000005597
AA Change: K816E

PolyPhen 2 Score 0.854 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000005597
Gene: ENSMUSG00000056592
AA Change: K816E

Domain	Start	End	E-Value	Type
KRAB	23	83	3.7e-27	SMART
ZnF_C2H2	276	295	8.84e1	SMART
ZnF_C2H2	301	323	5.81e-2	SMART
ZnF_C2H2	328	350	1.5e-4	SMART
ZnF_C2H2	356	378	1.84e-4	SMART
ZnF_C2H2	384	406	3.16e-3	SMART
ZnF_C2H2	412	434	1.45e-2	SMART
ZnF_C2H2	440	462	4.87e-4	SMART
ZnF_C2H2	468	490	4.87e-4	SMART
ZnF_C2H2	496	518	6.88e-4	SMART
ZnF_C2H2	524	546	1.69e-3	SMART
ZnF_C2H2	552	574	9.73e-4	SMART
ZnF_C2H2	580	602	1.82e-3	SMART
ZnF_C2H2	608	630	3.16e-3	SMART
ZnF_C2H2	636	658	8.94e-3	SMART
ZnF_C2H2	664	686	3.74e-5	SMART
ZnF_C2H2	692	714	2.99e-4	SMART
ZnF_C2H2	720	742	5.21e-4	SMART
ZnF_C2H2	748	770	2.79e-4	SMART
ZnF_C2H2	776	798	1.58e-3	SMART
ZnF_C2H2	804	826	3.44e-4	SMART
ZnF_C2H2	832	854	5.59e-4	SMART
ZnF_C2H2	860	882	5.81e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000058104

SMART Domains

Protein: ENSMUSP00000050968
Gene: ENSMUSG00000030469

Domain	Start	End	E-Value	Type
KRAB	49	109	1.18e-20	SMART
ZnF_C2H2	285	305	4.16e1	SMART
ZnF_C2H2	341	363	1.2e-3	SMART
ZnF_C2H2	369	391	9.08e-4	SMART
ZnF_C2H2	397	419	6.67e-2	SMART
ZnF_C2H2	425	447	7.9e-4	SMART
ZnF_C2H2	453	475	3.34e-2	SMART
ZnF_C2H2	481	503	1.38e-3	SMART
ZnF_C2H2	509	531	5.42e-2	SMART
ZnF_C2H2	537	559	3.78e-1	SMART
ZnF_C2H2	565	587	2.43e-4	SMART
ZnF_C2H2	593	615	4.54e-4	SMART
ZnF_C2H2	621	643	4.24e-4	SMART
ZnF_C2H2	649	671	6.42e-4	SMART
ZnF_C2H2	677	699	1.47e-3	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107972
AA Change: K816E

PolyPhen 2 Score 0.854 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000103606
Gene: ENSMUSG00000056592
AA Change: K816E

Domain	Start	End	E-Value	Type
KRAB	23	83	3.7e-27	SMART
ZnF_C2H2	276	295	8.84e1	SMART
ZnF_C2H2	301	323	5.81e-2	SMART
ZnF_C2H2	328	350	1.5e-4	SMART
ZnF_C2H2	356	378	1.84e-4	SMART
ZnF_C2H2	384	406	3.16e-3	SMART
ZnF_C2H2	412	434	1.45e-2	SMART
ZnF_C2H2	440	462	4.87e-4	SMART
ZnF_C2H2	468	490	4.87e-4	SMART
ZnF_C2H2	496	518	6.88e-4	SMART
ZnF_C2H2	524	546	1.69e-3	SMART
ZnF_C2H2	552	574	9.73e-4	SMART
ZnF_C2H2	580	602	1.82e-3	SMART
ZnF_C2H2	608	630	3.16e-3	SMART
ZnF_C2H2	636	658	8.94e-3	SMART
ZnF_C2H2	664	686	3.74e-5	SMART
ZnF_C2H2	692	714	2.99e-4	SMART
ZnF_C2H2	720	742	5.21e-4	SMART
ZnF_C2H2	748	770	2.79e-4	SMART
ZnF_C2H2	776	798	1.58e-3	SMART
ZnF_C2H2	804	826	3.44e-4	SMART
ZnF_C2H2	832	854	5.59e-4	SMART
ZnF_C2H2	860	882	5.81e-2	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000205769

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actbl2	T	A	13: 111,392,490 (GRCm39)	I275N	probably damaging	Het
Actr3b	A	C	5: 26,003,461 (GRCm39)	Y21S	probably damaging	Het
Adam6b	C	T	12: 113,453,662 (GRCm39)	Q160*	probably null	Het
Adgrv1	C	T	13: 81,670,223 (GRCm39)		probably null	Het
Akr1c6	A	G	13: 4,504,514 (GRCm39)	N300D	probably benign	Het
Alox8	T	C	11: 69,082,416 (GRCm39)	D170G	possibly damaging	Het
Amhr2	A	G	15: 102,354,768 (GRCm39)	N40S	probably benign	Het
Ap2a2	A	G	7: 141,209,109 (GRCm39)	N767S	probably benign	Het
Armc3	T	C	2: 19,274,839 (GRCm39)	I358T	probably damaging	Het
Atg2b	A	G	12: 105,620,508 (GRCm39)	S732P	probably benign	Het
Atp12a	A	G	14: 56,610,837 (GRCm39)	Y327C	possibly damaging	Het
Bcr	T	C	10: 74,897,393 (GRCm39)	V179A	probably benign	Het
Cep104	T	C	4: 154,078,018 (GRCm39)	L642P	probably benign	Het
Clspn	T	A	4: 126,486,513 (GRCm39)	S1302R	possibly damaging	Het
Cmip	T	C	8: 118,111,727 (GRCm39)	F153L	probably benign	Het
Cplane1	T	G	15: 8,258,246 (GRCm39)	L2164R	probably damaging	Het
D630039A03Rik	T	C	4: 57,910,521 (GRCm39)	D97G	probably damaging	Het
Dok7	A	T	5: 35,236,899 (GRCm39)	T396S	probably benign	Het
Dsel	C	T	1: 111,788,025 (GRCm39)	V837I	probably benign	Het
Etl4	C	T	2: 20,810,695 (GRCm39)	T926I	probably benign	Het
Gpatch2l	G	A	12: 86,290,958 (GRCm39)	R47H	probably damaging	Het
Gpr155	A	G	2: 73,173,961 (GRCm39)	I816T	probably damaging	Het
Hpn	C	T	7: 30,810,367 (GRCm39)		probably benign	Het
Inpp5e	A	G	2: 26,287,877 (GRCm39)	S640P	probably benign	Het
Irs3	C	T	5: 137,643,539 (GRCm39)	V82I	probably benign	Het
Kif16b	A	C	2: 142,600,749 (GRCm39)	D461E	possibly damaging	Het
Krba1	A	G	6: 48,390,014 (GRCm39)	T592A	possibly damaging	Het
Lgsn	T	A	1: 31,243,024 (GRCm39)	S369T	possibly damaging	Het
Lrrc4b	C	T	7: 44,094,580 (GRCm39)	P83S	probably damaging	Het
Mplkipl1	A	G	19: 61,164,319 (GRCm39)	S39P	possibly damaging	Het
Neil3	T	C	8: 54,054,001 (GRCm39)	T343A	possibly damaging	Het
Nt5e	A	G	9: 88,246,805 (GRCm39)	Y347C	probably damaging	Het
Or1j1	A	G	2: 36,703,047 (GRCm39)	I19T	possibly damaging	Het
Or2d3b	A	T	7: 106,514,319 (GRCm39)	T305S	probably benign	Het
Or2f2	C	T	6: 42,767,399 (GRCm39)	T142I	probably benign	Het
Or8g26	A	T	9: 39,096,239 (GRCm39)	Y255F	probably benign	Het
P2rx5	T	C	11: 73,058,800 (GRCm39)		probably null	Het
Phtf2	A	G	5: 20,999,399 (GRCm39)	V248A	probably benign	Het
Plekhd1	T	C	12: 80,768,734 (GRCm39)	C406R	possibly damaging	Het
Plod3	T	A	5: 137,018,498 (GRCm39)	N245K	probably damaging	Het
Polr3c	G	T	3: 96,630,954 (GRCm39)	H155Q	possibly damaging	Het
Psap	T	A	10: 60,135,276 (GRCm39)	C317S	probably damaging	Het
Rif1	A	G	2: 51,967,001 (GRCm39)	I97V	probably benign	Het
Rnf123	T	A	9: 107,940,882 (GRCm39)		probably null	Het
Rnf19a	G	A	15: 36,254,650 (GRCm39)	R303*	probably null	Het
Sdk1	G	A	5: 142,082,489 (GRCm39)	V1036I	probably benign	Het
Shc3	T	A	13: 51,620,588 (GRCm39)	Y146F	probably benign	Het
Skint6	A	G	4: 112,963,109 (GRCm39)	Y441H	probably benign	Het
Slc7a12	T	C	3: 14,570,580 (GRCm39)	I173T	probably damaging	Het
Spesp1	A	T	9: 62,189,302 (GRCm39)	S15T	possibly damaging	Het
Tarm1	A	T	7: 3,545,939 (GRCm39)		probably null	Het
Tenm3	T	C	8: 48,693,479 (GRCm39)	Y1817C	probably damaging	Het
Ttc9c	A	T	19: 8,795,904 (GRCm39)	L45Q	probably damaging	Het
Ube3a	C	T	7: 58,926,188 (GRCm39)	T322I	probably damaging	Het
Vac14	T	A	8: 111,439,430 (GRCm39)	V669E	probably damaging	Het
Vmn1r225	A	G	17: 20,723,416 (GRCm39)	M286V	probably null	Het
Zfp846	T	C	9: 20,499,188 (GRCm39)	M1T	probably null	Het

Other mutations in Zfp658

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Zfp658	APN	7	43,216,780 (GRCm39)	missense	probably benign	0.03
IGL00475:Zfp658	APN	7	43,223,500 (GRCm39)	missense	possibly damaging	0.68
IGL01972:Zfp658	APN	7	43,222,134 (GRCm39)	nonsense	probably null
IGL03223:Zfp658	APN	7	43,216,735 (GRCm39)	missense	possibly damaging	0.85
R0135:Zfp658	UTSW	7	43,223,019 (GRCm39)	nonsense	probably null
R1863:Zfp658	UTSW	7	43,223,323 (GRCm39)	missense	possibly damaging	0.78
R1962:Zfp658	UTSW	7	43,223,245 (GRCm39)	missense	possibly damaging	0.93
R2698:Zfp658	UTSW	7	43,222,969 (GRCm39)	missense	possibly damaging	0.53
R3781:Zfp658	UTSW	7	43,223,270 (GRCm39)	missense	probably benign
R4791:Zfp658	UTSW	7	43,223,890 (GRCm39)	missense	possibly damaging	0.93
R5392:Zfp658	UTSW	7	43,222,355 (GRCm39)	missense	probably benign	0.07
R6092:Zfp658	UTSW	7	43,223,951 (GRCm39)	missense	possibly damaging	0.73
R6594:Zfp658	UTSW	7	43,216,701 (GRCm39)	missense	possibly damaging	0.86
R7008:Zfp658	UTSW	7	43,223,336 (GRCm39)	missense	possibly damaging	0.95
R7077:Zfp658	UTSW	7	43,223,413 (GRCm39)	missense	probably benign	0.32
R7689:Zfp658	UTSW	7	43,224,102 (GRCm39)	missense	probably benign	0.00
R7793:Zfp658	UTSW	7	43,224,108 (GRCm39)	missense	possibly damaging	0.74
R7939:Zfp658	UTSW	7	43,224,301 (GRCm39)	missense	possibly damaging	0.73
R8672:Zfp658	UTSW	7	43,222,919 (GRCm39)	missense	possibly damaging	0.52
R8828:Zfp658	UTSW	7	43,222,240 (GRCm39)	missense	probably benign
R8995:Zfp658	UTSW	7	43,222,798 (GRCm39)	missense	possibly damaging	0.93
R9021:Zfp658	UTSW	7	43,223,381 (GRCm39)	missense	possibly damaging	0.71
R9259:Zfp658	UTSW	7	43,224,280 (GRCm39)	missense	probably benign	0.02
R9551:Zfp658	UTSW	7	43,222,567 (GRCm39)	missense	probably benign	0.00
R9552:Zfp658	UTSW	7	43,222,567 (GRCm39)	missense	probably benign	0.00
R9571:Zfp658	UTSW	7	43,222,139 (GRCm39)	missense	possibly damaging	0.85
Z1176:Zfp658	UTSW	7	43,222,641 (GRCm39)	missense	possibly damaging	0.72

Predicted Primers

PCR Primer
(F):5'- GGCTTCAAAGGGCCAACTTC -3'
(R):5'- GCATTGCTAAGGCTTCTTGC -3'

Sequencing Primer
(F):5'- TCGAGATCATCAGCGGATTC -3'
(R):5'- GAGTCCGCTGATGGCATTTCAAC -3'

Posted On

2019-05-13