Mutagenetix > Incidental Mutation

Incidental Mutation 'R0609:Rora'

54537

Institutional Source

Beutler Lab

Gene Symbol

Rora

Ensembl Gene

ENSMUSG00000032238

Gene Name

RAR-related orphan receptor alpha

Synonyms

tmgc26, Nr1f1, 9530021D13Rik

MMRRC Submission

038798-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.854) question?

Stock #

R0609 (G1)

Quality Score

190

Status

Not validated

Chromosome

Chromosomal Location

68653786-69388246 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 69361869 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 82 (M82K)

Ref Sequence

ENSEMBL: ENSMUSP00000109254 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000034766] [ENSMUST00000113624] [ENSMUST00000174296]

AlphaFold

P51448

Predicted Effect

probably damaging
Transcript: ENSMUST00000034766
AA Change: M138K

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000034766
Gene: ENSMUSG00000032238
AA Change: M138K

Domain	Start	End	E-Value	Type
low complexity region	2	26	N/A	INTRINSIC
ZnF_C4	70	141	4.71e-41	SMART
low complexity region	161	175	N/A	INTRINSIC
HOLI	325	481	8.8e-32	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000113624
AA Change: M82K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000109254
Gene: ENSMUSG00000032238
AA Change: M82K

Domain	Start	End	E-Value	Type
ZnF_C4	14	85	4.71e-41	SMART
low complexity region	105	119	N/A	INTRINSIC
HOLI	269	425	8.8e-32	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152570

Predicted Effect

probably benign
Transcript: ENSMUST00000174296

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.6%
20x: 95.6%

Validation Efficiency

MGI Phenotype

FUNCTION: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2014]
PHENOTYPE: Homozygotes for null mutations exhibit ataxia, cerebellar dysgenesis, impaired Purkinje and granule cell development, olfactory defects, hypoalphalipoproteinemia, and death around 4 weeks. Heterozygotes show slow Purkinje cell dedritic atrophy and loss. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 103 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930596D02Rik	A	T	14: 35,811,461 (GRCm38)		probably null	Het
Abcb4	T	A	5: 8,947,376 (GRCm38)	C952S	probably damaging	Het
Adamtsl2	A	G	2: 27,089,635 (GRCm38)	D272G	probably benign	Het
Aim2	G	A	1: 173,461,964 (GRCm38)	D158N	probably damaging	Het
Aldh3b1	C	T	19: 3,914,024 (GRCm38)	R426H	probably damaging	Het
Apoc2	A	G	7: 19,673,353 (GRCm38)	S28P	probably benign	Het
Arfgef3	G	A	10: 18,597,431 (GRCm38)	T1628I	probably benign	Het
Atp10a	G	A	7: 58,819,740 (GRCm38)		probably null	Het
Bcl2	G	A	1: 106,712,562 (GRCm38)	R107C	probably damaging	Het
Bmp8b	T	A	4: 123,121,899 (GRCm38)	D226E	probably benign	Het
Brsk2	T	C	7: 141,998,492 (GRCm38)	Y618H	probably damaging	Het
Casp12	T	A	9: 5,346,554 (GRCm38)	F27Y	probably damaging	Het
Casp8	T	A	1: 58,844,792 (GRCm38)	N439K	probably benign	Het
Ccdc175	T	A	12: 72,157,507 (GRCm38)	K253N	probably benign	Het
Cdc42bpa	A	G	1: 180,040,179 (GRCm38)	H193R	probably damaging	Het
Cdk17	T	C	10: 93,216,472 (GRCm38)	M105T	probably benign	Het
Cdon	C	A	9: 35,478,611 (GRCm38)	P854T	probably damaging	Het
Cep44	A	G	8: 56,544,152 (GRCm38)	M117T	possibly damaging	Het
Cep89	A	T	7: 35,435,530 (GRCm38)	E674D	probably damaging	Het
Cit	C	T	5: 115,873,943 (GRCm38)	A203V	probably damaging	Het
Clstn1	C	A	4: 149,629,300 (GRCm38)		probably null	Het
Col7a1	T	A	9: 108,958,147 (GRCm38)	D565E	unknown	Het
Cpb1	T	A	3: 20,262,474 (GRCm38)	Y304F	probably damaging	Het
Cps1	T	G	1: 67,172,802 (GRCm38)	Y710D	probably damaging	Het
Creb3l1	T	C	2: 91,987,053 (GRCm38)	T372A	possibly damaging	Het
Dars	A	G	1: 128,405,381 (GRCm38)	V102A	probably benign	Het
Dhx35	C	T	2: 158,817,415 (GRCm38)	T168I	possibly damaging	Het
Dnah5	T	C	15: 28,327,779 (GRCm38)	S2100P	probably benign	Het
Dst	T	C	1: 34,266,960 (GRCm38)		probably null	Het
Egflam	A	C	15: 7,253,523 (GRCm38)	L351R	possibly damaging	Het
Elp2	T	A	18: 24,626,156 (GRCm38)	D523E	probably benign	Het
Exo5	C	A	4: 120,921,684 (GRCm38)	G328V	probably damaging	Het
Fam208a	C	T	14: 27,461,750 (GRCm38)	T722I	probably benign	Het
Fut9	A	G	4: 25,620,811 (GRCm38)	M1T	probably null	Het
Galnt5	A	G	2: 58,024,625 (GRCm38)	N584S	possibly damaging	Het
Gbp3	T	C	3: 142,567,772 (GRCm38)	V360A	probably damaging	Het
Gdf6	G	A	4: 9,859,977 (GRCm38)	C353Y	probably damaging	Het
Gm13089	A	T	4: 143,698,503 (GRCm38)	D123E	probably benign	Het
Hace1	A	G	10: 45,648,869 (GRCm38)	T244A	probably damaging	Het
Hr	T	C	14: 70,559,657 (GRCm38)	I500T	probably benign	Het
Ifnl2	A	T	7: 28,509,282 (GRCm38)	L115Q	probably damaging	Het
Iigp1	T	C	18: 60,389,824 (GRCm38)	F5L	probably benign	Het
Inhbb	A	G	1: 119,417,416 (GRCm38)	L381P	probably damaging	Het
Irx3	A	T	8: 91,801,093 (GRCm38)	S50T	probably benign	Het
Ivns1abp	C	T	1: 151,360,145 (GRCm38)	T363I	probably benign	Het
Izumo1	A	T	7: 45,622,899 (GRCm38)	T35S	probably benign	Het
Kank4	A	G	4: 98,777,105 (GRCm38)	S651P	probably damaging	Het
Kit	T	C	5: 75,610,879 (GRCm38)	V232A	probably benign	Het
Klhl11	T	C	11: 100,463,714 (GRCm38)	Y427C	probably damaging	Het
Laptm4b	T	A	15: 34,258,689 (GRCm38)	N36K	probably damaging	Het
Lrrk1	T	C	7: 66,266,615 (GRCm38)		probably null	Het
Mamdc4	G	T	2: 25,564,193 (GRCm38)	Q1042K	probably benign	Het
Mical2	A	G	7: 112,321,440 (GRCm38)		probably null	Het
Ms4a3	C	A	19: 11,631,361 (GRCm38)	V176F	possibly damaging	Het
Myo3a	A	C	2: 22,396,299 (GRCm38)	E626D	possibly damaging	Het
Myo3a	T	C	2: 22,333,513 (GRCm38)	V427A	probably benign	Het
Nckap5	A	T	1: 126,027,288 (GRCm38)	L509*	probably null	Het
Ndufa5	A	T	6: 24,519,249 (GRCm38)	D64E	possibly damaging	Het
Nedd4l	T	C	18: 65,208,461 (GRCm38)	Y753H	probably damaging	Het
Nynrin	T	C	14: 55,872,761 (GRCm38)	V1775A	probably damaging	Het
Olfr141	A	G	2: 86,806,861 (GRCm38)	L46S	probably damaging	Het
Olfr292	T	C	7: 86,694,876 (GRCm38)	V140A	possibly damaging	Het
Olfr694	A	T	7: 106,688,998 (GRCm38)	H244Q	probably damaging	Het
Olfr735	T	C	14: 50,345,926 (GRCm38)	Y141C	probably damaging	Het
Olfr823	G	A	10: 130,112,580 (GRCm38)	S70F	probably damaging	Het
Oplah	A	G	15: 76,302,992 (GRCm38)	S570P	probably benign	Het
Osbpl11	C	A	16: 33,234,444 (GRCm38)	Y632*	probably null	Het
Osbpl5	A	T	7: 143,694,821 (GRCm38)	L644Q	probably damaging	Het
Pcdhb19	T	C	18: 37,497,952 (GRCm38)	W267R	probably benign	Het
Pkhd1l1	A	C	15: 44,467,424 (GRCm38)	S132R	possibly damaging	Het
Ptpn13	T	A	5: 103,556,145 (GRCm38)	S1348T	probably benign	Het
Rc3h1	T	A	1: 160,930,135 (GRCm38)	W8R	probably damaging	Het
Rgs3	T	G	4: 62,625,936 (GRCm38)	V315G	probably damaging	Het
Rph3al	T	C	11: 75,908,969 (GRCm38)	I55V	probably benign	Het
Sag	T	C	1: 87,812,991 (GRCm38)	V45A	probably damaging	Het
Scn3a	T	C	2: 65,536,510 (GRCm38)	E56G	probably damaging	Het
Sec24c	T	G	14: 20,686,948 (GRCm38)	V324G	probably damaging	Het
Sptbn1	A	G	11: 30,138,979 (GRCm38)	L748S	probably damaging	Het
Stard9	A	T	2: 120,706,306 (GRCm38)	D4186V	probably damaging	Het
Stk39	T	C	2: 68,366,167 (GRCm38)	E306G	probably damaging	Het
Sycp1	C	A	3: 102,898,849 (GRCm38)		probably null	Het
Taf2	A	C	15: 55,060,050 (GRCm38)	L277R	probably damaging	Het
Tbc1d23	T	A	16: 57,173,106 (GRCm38)	I566F	possibly damaging	Het
Tekt5	T	C	16: 10,361,304 (GRCm38)	T400A	possibly damaging	Het
Tgfbrap1	T	C	1: 43,060,141 (GRCm38)	H401R	probably benign	Het
Tie1	T	A	4: 118,476,147 (GRCm38)	I841L	possibly damaging	Het
Tln1	T	G	4: 43,544,645 (GRCm38)	T1095P	possibly damaging	Het
Tmem147	A	G	7: 30,728,102 (GRCm38)	Y72H	probably benign	Het
Tnfaip2	A	G	12: 111,453,507 (GRCm38)	N691S	probably benign	Het
Trim24	G	A	6: 37,957,783 (GRCm38)	C811Y	probably damaging	Het
Trim30b	T	A	7: 104,357,976 (GRCm38)		probably benign	Het
Trpc4	T	G	3: 54,194,768 (GRCm38)	L29R	probably damaging	Het
Trpm6	A	T	19: 18,825,862 (GRCm38)	I890F	probably damaging	Het
Ttc23l	C	T	15: 10,504,536 (GRCm38)	E442K	probably benign	Het
Uggt2	A	G	14: 119,095,336 (GRCm38)	V62A	probably damaging	Het
Ugt1a6a	C	A	1: 88,138,884 (GRCm38)	S137R	probably benign	Het
Unc13a	A	G	8: 71,658,467 (GRCm38)	Y367H	probably damaging	Het
Vmn2r49	A	G	7: 9,976,306 (GRCm38)	I833T	probably benign	Het
Vmn2r7	T	C	3: 64,716,479 (GRCm38)	D231G	probably benign	Het
Ythdc2	A	T	18: 44,864,357 (GRCm38)	M994L	probably benign	Het
Zcchc6	A	T	13: 59,799,782 (GRCm38)	C506*	probably null	Het
Zfp804a	G	A	2: 82,257,588 (GRCm38)	S587N	probably damaging	Het
Zswim2	A	G	2: 83,923,659 (GRCm38)	I219T	probably benign	Het

Other mutations in Rora

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00811:Rora	APN	9	69,371,290 (GRCm38)	missense	probably benign	0.31
IGL02355:Rora	APN	9	69,374,092 (GRCm38)	missense	probably damaging	1.00
IGL02362:Rora	APN	9	69,374,092 (GRCm38)	missense	probably damaging	1.00
PIT4696001:Rora	UTSW	9	69,364,559 (GRCm38)	missense	possibly damaging	0.92
R0091:Rora	UTSW	9	69,374,048 (GRCm38)	missense	probably damaging	1.00
R0555:Rora	UTSW	9	69,361,746 (GRCm38)	missense	probably damaging	1.00
R1483:Rora	UTSW	9	69,364,385 (GRCm38)	missense	probably benign	0.00
R1712:Rora	UTSW	9	69,375,489 (GRCm38)	missense	probably benign	0.23
R1785:Rora	UTSW	9	69,376,837 (GRCm38)	missense	probably benign	0.30
R2883:Rora	UTSW	9	69,375,435 (GRCm38)	missense	probably damaging	1.00
R4173:Rora	UTSW	9	68,653,910 (GRCm38)	missense	probably benign	0.41
R5226:Rora	UTSW	9	69,364,141 (GRCm38)	intron	probably benign
R5660:Rora	UTSW	9	68,653,921 (GRCm38)	missense	probably benign	0.27
R6029:Rora	UTSW	9	69,364,452 (GRCm38)	missense	probably benign	0.04
R6054:Rora	UTSW	9	69,378,802 (GRCm38)	missense	probably benign	0.04
R6114:Rora	UTSW	9	69,371,323 (GRCm38)	missense	probably benign
R6329:Rora	UTSW	9	69,373,186 (GRCm38)	missense	probably damaging	1.00
R7028:Rora	UTSW	9	69,196,083 (GRCm38)	missense	possibly damaging	0.46
R7170:Rora	UTSW	9	69,373,190 (GRCm38)	nonsense	probably null
R7233:Rora	UTSW	9	69,197,522 (GRCm38)	nonsense	probably null
R7512:Rora	UTSW	9	69,374,085 (GRCm38)	missense	probably benign	0.00
R9647:Rora	UTSW	9	69,348,168 (GRCm38)	missense	probably damaging	1.00
Z1176:Rora	UTSW	9	69,364,372 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- TTGGGTATCACCGCTGGAACTGAC -3'
(R):5'- TGCATGAAAGCCCTCAGATGACAC -3'

Sequencing Primer
(F):5'- GCTGGAACTGACACTAACCTG -3'
(R):5'- CCTGAAGCTATTCTGAGTACAGGAC -3'

Posted On

2013-07-11