Mutagenetix > Incidental Mutation

Incidental Mutation 'R7895:Olfr169'

609636

Institutional Source

Beutler Lab

Gene Symbol

Olfr169

Ensembl Gene

ENSMUSG00000068535

Gene Name

olfactory receptor 169

Synonyms

GA_x54KRFPKG5P-16014972-16014031, MOR273-3P

MMRRC Submission

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.057) question?

Stock #

R7895 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

19564889-19571809 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to A at 19566722 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Stop codon at position 54 (R54*)

Ref Sequence

ENSEMBL: ENSMUSP00000149087 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090062] [ENSMUST00000215040] [ENSMUST00000215476] [ENSMUST00000216070]

AlphaFold

Q7TS53

Predicted Effect

probably null
Transcript: ENSMUST00000090062
AA Change: R54*

SMART Domains

Protein: ENSMUSP00000087516
Gene: ENSMUSG00000068535
AA Change: R54*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	28	308	1.3e-45	PFAM
Pfam:7TM_GPCR_Srsx	35	303	2e-6	PFAM
Pfam:7tm_1	41	290	7.4e-29	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000215040
AA Change: R54*

Predicted Effect

probably null
Transcript: ENSMUST00000215476
AA Change: R54*

Predicted Effect

probably null
Transcript: ENSMUST00000216070
AA Change: R54*

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.1%

Validation Efficiency

99% (92/93)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 92 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700097O09Rik	A	T	12: 55,059,510	S143T	probably benign	Het
9030624G23Rik	A	T	12: 24,044,723	M149K	unknown	Het
Agpat3	T	A	10: 78,283,200	I187F	probably benign	Het
Ahcyl1	C	T	3: 107,669,151	A332T	probably damaging	Het
Aldh3b3	A	G	19: 3,964,871	I123V	possibly damaging	Het
Ap5z1	T	A	5: 142,470,558		probably null	Het
Apob	A	T	12: 8,011,933	I3472F	probably benign	Het
Arhgef33	C	T	17: 80,373,485	P685S	probably benign	Het
Arhgef4	T	C	1: 34,806,397	I130T	probably damaging	Het
Arnt2	A	T	7: 84,305,198	F263I	probably benign	Het
Bbof1	A	G	12: 84,419,989	R177G	probably damaging	Het
Bbs2	C	A	8: 94,081,136	G372W	probably damaging	Het
Blmh	T	A	11: 76,945,895		probably null	Het
Ccdc30	A	T	4: 119,352,713		probably null	Het
Cdca7l	G	T	12: 117,873,732	L219F	probably damaging	Het
Cenpu	C	A	8: 46,562,464	A138E	probably benign	Het
Cfap65	T	A	1: 74,933,162	T13S	probably benign	Het
Clasp2	A	G	9: 113,903,948	M951V	probably benign	Het
Clca4a	A	G	3: 144,968,405	S190P	probably benign	Het
Clcn4	A	G	7: 7,295,168	V74A	probably benign	Het
Cntln	C	T	4: 85,063,324	T913M	possibly damaging	Het
Cntnap4	T	A	8: 112,752,197	V185E	probably damaging	Het
Col26a1	C	T	5: 136,748,177		probably null	Het
Ctcf	A	T	8: 105,664,058	Q99L	possibly damaging	Het
Dgke	T	C	11: 89,040,856	Q524R	probably damaging	Het
Dhrs7	A	T	12: 72,652,460		probably null	Het
Dnah7b	T	C	1: 46,249,950	Y2969H	probably damaging	Het
Dync1h1	T	G	12: 110,616,457	I358S	probably damaging	Het
Efcab3	A	C	11: 105,117,324	D410A	probably benign	Het
Emilin2	T	C	17: 71,273,913	D606G	probably benign	Het
Ermard	C	T	17: 15,063,613	T622I	possibly damaging	Het
Exo1	A	G	1: 175,900,996	D542G	probably benign	Het
Fgd5	A	T	6: 91,987,281	D165V	probably benign	Het
Gdap1	T	C	1: 17,161,144	W238R	probably damaging	Het
Gm11096	T	G	17: 81,441,899	I7M	unknown	Het
Gm40460	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	ACCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAGCCACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAGGAACTACAGCCTCCCTTGCAGCCCCCACAG	7: 142,240,713		probably benign	Het
Gm436	A	T	4: 144,670,343	I273K	possibly damaging	Het
Gmppb	A	T	9: 108,050,571	M175L	probably benign	Het
Gorasp2	G	A	2: 70,684,098	S273N	probably benign	Het
Gtf3c1	T	C	7: 125,672,822	M642V	possibly damaging	Het
Hmx2	T	A	7: 131,555,871	L238Q	probably damaging	Het
Kif13b	A	G	14: 64,736,149	D316G	probably damaging	Het
Kmt2c	A	G	5: 25,373,176	S701P	possibly damaging	Het
Lama4	T	A	10: 39,088,329	N1332K	probably damaging	Het
Lrp2	T	C	2: 69,458,479	D3681G	probably damaging	Het
Maml3	G	T	3: 51,697,722	P722Q	probably damaging	Het
Mier2	C	A	10: 79,541,885		probably benign	Het
Ms4a15	A	T	19: 10,979,330		probably null	Het
Msh5	T	C	17: 35,044,379	M158V	probably benign	Het
Nova2	A	T	7: 18,942,345	K136I		Het
Npm1	A	T	11: 33,156,001		probably null	Het
Nr4a3	T	A	4: 48,051,390	M48K	probably benign	Het
Nrap	T	G	19: 56,354,152	T806P	probably benign	Het
Ntf3	G	A	6: 126,102,240	T101M	probably benign	Het
Paxbp1	T	A	16: 91,025,278	D648V	probably damaging	Het
Pcdhb18	T	A	18: 37,490,467	D283E	probably benign	Het
Phrf1	C	A	7: 141,259,375	Q828K	unknown	Het
Pigq	A	T	17: 25,937,325	I43N	probably benign	Het
Pou6f2	G	T	13: 18,125,448	T542K		Het
Ppfibp2	T	A	7: 107,721,317		probably null	Het
Rab1b	A	T	19: 5,100,496	M163K	probably benign	Het
Ralgapa1	A	G	12: 55,747,149	M567T	probably benign	Het
Reck	T	C	4: 43,890,970	V36A	probably benign	Het
Resp18	C	T	1: 75,278,202	D36N	probably null	Het
Rint1	A	C	5: 23,800,722	H134P	probably damaging	Het
Rps19	A	T	7: 24,888,339	K77M	possibly damaging	Het
Sec24b	A	T	3: 129,995,949	S808T	probably benign	Het
Sema4c	A	C	1: 36,553,118	V216G	probably damaging	Het
Sept10	T	A	10: 59,181,049	T218S	probably benign	Het
Sept3	A	T	15: 82,285,819	H182L	probably benign	Het
Sipa1	A	G	19: 5,652,662	S836P	probably benign	Het
Slc9a5	A	G	8: 105,363,366	K652R	probably damaging	Het
Slco5a1	T	A	1: 12,989,703	I265F	possibly damaging	Het
Snapc5	T	A	9: 64,179,332	M1K	probably null	Het
Snx5	T	A	2: 144,253,820	D307V	possibly damaging	Het
Srebf2	A	T	15: 82,177,240	M381L	probably benign	Het
Ssh2	G	C	11: 77,454,626	E1146Q	probably benign	Het
Suco	A	T	1: 161,845,368		probably null	Het
Tanc2	T	A	11: 105,921,825	L1365Q	probably damaging	Het
Tas1r3	A	G	4: 155,862,548	L200P	probably damaging	Het
Thbs2	T	G	17: 14,676,221	D802A	probably damaging	Het
Trav3-1	G	A	14: 52,581,093	V75M	probably benign	Het
Trim9	G	A	12: 70,255,187	P559L	probably benign	Het
Trpc6	G	A	9: 8,655,218	G683D	probably damaging	Het
Unc5b	C	A	10: 60,779,730	V164L	possibly damaging	Het
Usf3	A	T	16: 44,216,202	R348S	possibly damaging	Het
Vmn1r236	T	C	17: 21,287,466	V282A	possibly damaging	Het
Vmn2r1	A	G	3: 64,089,709	E262G	possibly damaging	Het
Xirp2	A	G	2: 67,509,497	E694G	probably damaging	Het
Xpo4	T	A	14: 57,602,591	H572L	probably benign	Het
Zfhx4	A	G	3: 5,242,199	T162A	probably benign	Het
Zfp110	T	A	7: 12,837,093	H59Q	possibly damaging	Het

Other mutations in Olfr169

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01080:Olfr169	APN	16	19566208	missense	probably damaging	1.00
IGL01862:Olfr169	APN	16	19566676	missense	probably damaging	1.00
IGL02064:Olfr169	APN	16	19566548	missense	probably damaging	1.00
IGL03061:Olfr169	APN	16	19566713	missense	possibly damaging	0.87
IGL03136:Olfr169	APN	16	19566353	missense	probably damaging	1.00
R0066:Olfr169	UTSW	16	19566049	missense	probably damaging	1.00
R0243:Olfr169	UTSW	16	19566294	missense	probably damaging	0.97
R0629:Olfr169	UTSW	16	19565980	missense	possibly damaging	0.88
R1644:Olfr169	UTSW	16	19566406	missense	probably benign	0.11
R1943:Olfr169	UTSW	16	19566437	missense	probably benign	0.19
R3016:Olfr169	UTSW	16	19566391	missense	probably damaging	1.00
R4290:Olfr169	UTSW	16	19566244	missense	possibly damaging	0.88
R4689:Olfr169	UTSW	16	19566513	nonsense	probably null
R4791:Olfr169	UTSW	16	19566663	missense	possibly damaging	0.50
R5497:Olfr169	UTSW	16	19566330	missense	probably benign	0.10
R5843:Olfr169	UTSW	16	19566583	missense	probably damaging	1.00
R6106:Olfr169	UTSW	16	19566259	missense	probably damaging	0.99
R6249:Olfr169	UTSW	16	19565975	missense	probably damaging	0.99
R9284:Olfr169	UTSW	16	19566607	missense	probably damaging	1.00
R9335:Olfr169	UTSW	16	19566763	missense	probably benign	0.32
R9364:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68
R9404:Olfr169	UTSW	16	19565981	missense	probably benign	0.01
R9475:Olfr169	UTSW	16	19566520	missense	probably benign	0.09
R9554:Olfr169	UTSW	16	19565972	missense	possibly damaging	0.68

Predicted Primers

PCR Primer
(F):5'- TCGATCATAGGACATGGCTGC -3'
(R):5'- CTATGAGGCACTTGATGATGAATAC -3'

Sequencing Primer
(F):5'- GGAGAAGGCACTCACCACCTAG -3'
(R):5'- GATCTCTTTGCAGGTAAAATAGAAGG -3'

Posted On

2019-12-20