Mutagenetix > Incidental Mutation

Incidental Mutation 'R8078:Zfp760'

629164

Institutional Source

Beutler Lab

Gene Symbol

Zfp760

Ensembl Gene

ENSMUSG00000067928

Gene Name

zinc finger protein 760

Synonyms

MMRRC Submission

067512-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.135) question?

Stock #

R8078 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

21926723-21944617 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 21942436 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 537 (V537A)

Ref Sequence

ENSEMBL: ENSMUSP00000073038 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000073312]

AlphaFold

E9QAF5

Predicted Effect

probably benign
Transcript: ENSMUST00000073312
AA Change: V537A

PolyPhen 2 Score 0.266 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000073038
Gene: ENSMUSG00000067928
AA Change: V537A

Domain	Start	End	E-Value	Type
KRAB	8	69	3.62e-21	SMART
ZnF_C2H2	183	202	1.78e2	SMART
ZnF_C2H2	208	230	1.69e-3	SMART
ZnF_C2H2	236	258	4.54e-4	SMART
ZnF_C2H2	264	286	2.79e-4	SMART
ZnF_C2H2	292	314	1.1e-2	SMART
ZnF_C2H2	320	342	2.71e-2	SMART
ZnF_C2H2	348	370	7.9e-4	SMART
ZnF_C2H2	376	398	1.72e-4	SMART
ZnF_C2H2	404	426	1.14e0	SMART
ZnF_C2H2	432	454	4.05e-1	SMART
ZnF_C2H2	460	482	5.14e-3	SMART
ZnF_C2H2	488	510	1.18e-2	SMART
ZnF_C2H2	516	538	1.95e-3	SMART
ZnF_C2H2	544	566	1.82e-3	SMART
ZnF_C2H2	572	594	4.17e-3	SMART
ZnF_C2H2	600	622	1.04e-3	SMART
ZnF_C2H2	628	650	1.28e-3	SMART

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.8%
20x: 95.9%

Validation Efficiency

100% (63/63)

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	G	A	11: 9,251,279 (GRCm39)	V2884I	probably benign	Het
Actl10	T	A	2: 154,394,490 (GRCm39)	N147K	probably benign	Het
Agpat2	C	A	2: 26,494,113 (GRCm39)	C37F	possibly damaging	Het
Alb	G	C	5: 90,615,214 (GRCm39)	R242P	probably damaging	Het
Ankrd26	T	A	6: 118,494,854 (GRCm39)		probably null	Het
Bcas1	T	C	2: 170,260,532 (GRCm39)	E49G	possibly damaging	Het
Birc2	A	G	9: 7,858,742 (GRCm39)	V331A	probably damaging	Het
Cadm3	A	G	1: 173,168,626 (GRCm39)	V314A	probably damaging	Het
Cep290	T	C	10: 100,408,749 (GRCm39)	V2407A	probably benign	Het
Chd5	C	A	4: 152,445,448 (GRCm39)	N446K	possibly damaging	Het
Clec9a	A	G	6: 129,385,996 (GRCm39)	S27G	probably benign	Het
Coro1c	G	T	5: 114,020,164 (GRCm39)	A17E	probably damaging	Het
Cspg4	T	C	9: 56,797,543 (GRCm39)	S1336P	possibly damaging	Het
Ddx55	G	A	5: 124,704,451 (GRCm39)	V353M	probably damaging	Het
Farp1	T	A	14: 121,513,712 (GRCm39)	S836T	probably benign	Het
Fbll1	G	T	11: 35,688,728 (GRCm39)	D178E	probably benign	Het
Flg2	T	G	3: 93,107,582 (GRCm39)	D22E	probably damaging	Het
Glis1	T	A	4: 107,425,099 (GRCm39)	C237S	probably damaging	Het
Gm5519	T	C	19: 33,800,357 (GRCm39)	V17A	possibly damaging	Het
Gucy2c	C	A	6: 136,674,919 (GRCm39)	G1055C	probably damaging	Het
Hspg2	T	C	4: 137,235,333 (GRCm39)	Y123H	probably damaging	Het
Idh1	T	A	1: 65,200,225 (GRCm39)	I380F	probably damaging	Het
Il23r	A	T	6: 67,400,577 (GRCm39)	N584K	probably damaging	Het
Irag1	T	C	7: 110,498,942 (GRCm39)	E363G	probably damaging	Het
Lama1	A	T	17: 68,098,289 (GRCm39)	T1829S		Het
Ldlrad1	G	A	4: 107,066,688 (GRCm39)	A8T	probably benign	Het
Luzp2	T	A	7: 54,702,510 (GRCm39)	Y24*	probably null	Het
Masp2	A	T	4: 148,698,235 (GRCm39)	T439S	probably benign	Het
Meioc	G	A	11: 102,559,226 (GRCm39)	W49*	probably null	Het
Mtor	T	G	4: 148,552,744 (GRCm39)	I749S	probably benign	Het
Myo16	T	A	8: 10,612,078 (GRCm39)	V1241D	unknown	Het
Ncam2	G	T	16: 81,240,136 (GRCm39)	R199L	possibly damaging	Het
Nyap2	A	G	1: 81,218,772 (GRCm39)	S265G	possibly damaging	Het
Or4f15	T	A	2: 111,813,615 (GRCm39)	D268V	probably damaging	Het
Parp8	A	T	13: 117,061,519 (GRCm39)	F175I	probably damaging	Het
Pcnx1	A	G	12: 82,022,054 (GRCm39)	T823A		Het
Phc2	T	A	4: 128,604,855 (GRCm39)	I197N	probably damaging	Het
Plaat3	C	T	19: 7,556,526 (GRCm39)	T109I	probably benign	Het
Pot1a	T	C	6: 25,750,107 (GRCm39)	I518M	probably benign	Het
Prg2	T	A	2: 84,812,604 (GRCm39)	C105S	probably benign	Het
Rab39	A	G	9: 53,617,255 (GRCm39)	L54P	possibly damaging	Het
Rell1	T	A	5: 64,097,064 (GRCm39)		probably benign	Het
Repin1	G	T	6: 48,574,279 (GRCm39)	E403*	probably null	Het
Slc4a4	T	C	5: 89,327,566 (GRCm39)	Y668H	probably benign	Het
Sncg	C	A	14: 34,096,727 (GRCm39)		probably benign	Het
Spata31d1b	A	G	13: 59,863,263 (GRCm39)	D137G	probably damaging	Het
Tas1r1	C	T	4: 152,112,803 (GRCm39)	G750D	probably damaging	Het
Tcea3	A	T	4: 135,981,825 (GRCm39)	D61V	probably damaging	Het
Tfap2c	T	A	2: 172,393,392 (GRCm39)	H102Q	probably damaging	Het
Tmem150a	T	A	6: 72,335,306 (GRCm39)	L95H	probably damaging	Het
Tnfrsf11a	A	T	1: 105,745,409 (GRCm39)	D149V	probably damaging	Het
Tsc22d2	C	A	3: 58,323,453 (GRCm39)	A115E	probably benign	Het
Vmn2r101	T	A	17: 19,810,507 (GRCm39)	F431Y	probably benign	Het
Vmn2r111	T	C	17: 22,778,032 (GRCm39)	N549S	possibly damaging	Het
Vmn2r85	C	A	10: 130,265,364 (GRCm39)	E40*	probably null	Het
Zfp606	G	A	7: 12,214,942 (GRCm39)	A57T	possibly damaging	Het

Other mutations in Zfp760

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00472:Zfp760	APN	17	21,942,457 (GRCm39)	missense	possibly damaging	0.75
IGL00862:Zfp760	APN	17	21,941,265 (GRCm39)	missense	probably benign	0.00
IGL02198:Zfp760	APN	17	21,941,193 (GRCm39)	missense	probably benign	0.00
R0478:Zfp760	UTSW	17	21,940,995 (GRCm39)	nonsense	probably null
R0835:Zfp760	UTSW	17	21,942,559 (GRCm39)	missense	possibly damaging	0.63
R1191:Zfp760	UTSW	17	21,942,286 (GRCm39)	missense	probably damaging	1.00
R1760:Zfp760	UTSW	17	21,941,311 (GRCm39)	missense	probably damaging	1.00
R2698:Zfp760	UTSW	17	21,939,935 (GRCm39)	missense	probably damaging	0.99
R3722:Zfp760	UTSW	17	21,941,143 (GRCm39)	missense	probably damaging	1.00
R4561:Zfp760	UTSW	17	21,942,648 (GRCm39)	missense	probably benign	0.00
R4700:Zfp760	UTSW	17	21,941,388 (GRCm39)	missense	probably benign	0.01
R4859:Zfp760	UTSW	17	21,942,516 (GRCm39)	nonsense	probably null
R4859:Zfp760	UTSW	17	21,942,511 (GRCm39)	missense	probably damaging	0.97
R4897:Zfp760	UTSW	17	21,942,229 (GRCm39)	missense	probably benign	0.02
R6675:Zfp760	UTSW	17	21,941,991 (GRCm39)	missense	possibly damaging	0.92
R7286:Zfp760	UTSW	17	21,941,760 (GRCm39)	missense	probably benign	0.04
R7336:Zfp760	UTSW	17	21,942,814 (GRCm39)	missense	unknown
R7356:Zfp760	UTSW	17	21,941,601 (GRCm39)	missense	probably benign
R7369:Zfp760	UTSW	17	21,942,214 (GRCm39)	missense	probably benign	0.00
R7504:Zfp760	UTSW	17	21,941,655 (GRCm39)	missense	probably damaging	0.97
R7553:Zfp760	UTSW	17	21,941,872 (GRCm39)	missense	possibly damaging	0.82
R7577:Zfp760	UTSW	17	21,941,242 (GRCm39)	nonsense	probably null
R7579:Zfp760	UTSW	17	21,941,907 (GRCm39)	missense	possibly damaging	0.93
R7608:Zfp760	UTSW	17	21,941,797 (GRCm39)	missense	probably benign	0.00
R7973:Zfp760	UTSW	17	21,941,084 (GRCm39)	missense	probably benign	0.00
R8332:Zfp760	UTSW	17	21,942,301 (GRCm39)	missense	probably damaging	0.99
R8750:Zfp760	UTSW	17	21,941,356 (GRCm39)	missense	possibly damaging	0.56
R9094:Zfp760	UTSW	17	21,941,932 (GRCm39)	missense	possibly damaging	0.86
R9264:Zfp760	UTSW	17	21,942,663 (GRCm39)	missense	possibly damaging	0.80
R9372:Zfp760	UTSW	17	21,941,035 (GRCm39)	missense	probably benign	0.00
R9520:Zfp760	UTSW	17	21,941,036 (GRCm39)	missense	probably benign	0.00
R9564:Zfp760	UTSW	17	21,942,272 (GRCm39)	missense	possibly damaging	0.94
R9743:Zfp760	UTSW	17	21,942,338 (GRCm39)	missense	probably benign
X0057:Zfp760	UTSW	17	21,942,663 (GRCm39)	missense	possibly damaging	0.80

Predicted Primers

PCR Primer
(F):5'- GTGCCTTTCTCAGTTGTCAAAAC -3'
(R):5'- CTTTGTAAGAAGAGAGCGCTG -3'

Sequencing Primer
(F):5'- AAAAGCCATTATAGAATCCATACAGG -3'
(R):5'- TACCCCAGCATGAATTTTCTGATGAG -3'

Posted On

2020-06-30