Mutagenetix > Incidental Mutation

Incidental Mutation 'R0053:Lzts2'

64351

Institutional Source

Beutler Lab

Gene Symbol

Lzts2

Ensembl Gene

ENSMUSG00000035342

Gene Name

leucine zipper, putative tumor suppressor 2

Synonyms

MMRRC Submission

038347-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.126) question?

Stock #

R0053 (G1)

Quality Score

171

Status

Validated

Chromosome

Chromosomal Location

45003615-45015554 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to C at 45014746 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000133273 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039016] [ENSMUST00000145391] [ENSMUST00000169459] [ENSMUST00000178087] [ENSMUST00000179108]

AlphaFold

Q91YU6

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000038901

Predicted Effect

unknown
Transcript: ENSMUST00000039016
AA Change: I613T

SMART Domains

Protein: ENSMUSP00000045478
Gene: ENSMUSG00000035342
AA Change: I613T

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	639	4.2e-82	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000145391

SMART Domains

Protein: ENSMUSP00000119002
Gene: ENSMUSG00000074818

Domain	Start	End	E-Value	Type
low complexity region	12	35	N/A	INTRINSIC
PDZ	95	167	3.51e-19	SMART
PDZ	220	292	2.47e-14	SMART
low complexity region	319	344	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	521	533	N/A	INTRINSIC
low complexity region	724	744	N/A	INTRINSIC
low complexity region	768	809	N/A	INTRINSIC
low complexity region	812	824	N/A	INTRINSIC
PDZ	866	947	1.96e-8	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000169459

SMART Domains

Protein: ENSMUSP00000133273
Gene: ENSMUSG00000074818

Domain	Start	End	E-Value	Type
low complexity region	12	35	N/A	INTRINSIC
PDZ	95	167	3.51e-19	SMART
PDZ	220	292	2.47e-14	SMART
low complexity region	319	344	N/A	INTRINSIC
low complexity region	442	459	N/A	INTRINSIC
low complexity region	521	533	N/A	INTRINSIC
low complexity region	724	744	N/A	INTRINSIC
low complexity region	768	809	N/A	INTRINSIC
low complexity region	812	824	N/A	INTRINSIC
PDZ	866	947	1.96e-8	SMART

Predicted Effect

unknown
Transcript: ENSMUST00000178087
AA Change: I613T

SMART Domains

Protein: ENSMUSP00000136405
Gene: ENSMUSG00000035342
AA Change: I613T

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	638	2.2e-66	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000179108
AA Change: I613T

SMART Domains

Protein: ENSMUSP00000137571
Gene: ENSMUSG00000035342
AA Change: I613T

Domain	Start	End	E-Value	Type
low complexity region	99	105	N/A	INTRINSIC
low complexity region	187	199	N/A	INTRINSIC
low complexity region	242	265	N/A	INTRINSIC
low complexity region	267	321	N/A	INTRINSIC
low complexity region	375	407	N/A	INTRINSIC
low complexity region	412	423	N/A	INTRINSIC
Pfam:Fez1	441	639	4.2e-82	PFAM

Meta Mutation Damage Score

0.7307

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.9%
20x: 96.5%

Validation Efficiency

100% (67/67)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the leucine zipper tumor suppressor family of proteins, which function in transcription regulation and cell cycle control. This family member can repress beta-catenin-mediated transcriptional activation and is a negative regulator of the Wnt signaling pathway. It negatively regulates microtubule severing at centrosomes, and is necessary for central spindle formation and cytokinesis completion. It is implicated in cancer, where it may inhibit cell proliferation and decrease susceptibility to tumor development. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2015]
PHENOTYPE: Mice homozygous for a null mutation display defects in urinary tract development. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ada	A	T	2: 163,574,212 (GRCm39)	V148D	probably damaging	Het
Alpi	T	C	1: 87,026,512 (GRCm39)	D493G	probably benign	Het
Atp10b	A	G	11: 43,107,391 (GRCm39)		probably benign	Het
AY761185	A	T	8: 21,434,546 (GRCm39)		probably benign	Het
Cadm1	C	T	9: 47,710,712 (GRCm39)	T205I	probably damaging	Het
Capn3	A	G	2: 120,322,318 (GRCm39)	I413V	possibly damaging	Het
Cblb	C	T	16: 51,963,164 (GRCm39)	T369I	probably damaging	Het
Ccdc54	T	A	16: 50,410,597 (GRCm39)	N223I	probably benign	Het
Cdc25c	A	G	18: 34,868,488 (GRCm39)	V294A	probably benign	Het
Cep170	A	T	1: 176,609,946 (GRCm39)	S122T	possibly damaging	Het
Chd1	A	G	17: 15,967,451 (GRCm39)	N849D	probably damaging	Het
Cspg4b	T	C	13: 113,505,023 (GRCm39)	W2051R	probably benign	Het
Dpp3	A	G	19: 4,973,154 (GRCm39)	C147R	probably damaging	Het
Dst	A	G	1: 34,333,631 (GRCm39)		probably null	Het
Fbxw9	T	A	8: 85,791,083 (GRCm39)	L250Q	probably damaging	Het
Gpr75	A	T	11: 30,842,571 (GRCm39)	Q492L	possibly damaging	Het
Gramd4	T	A	15: 86,014,339 (GRCm39)		probably benign	Het
Hivep2	T	C	10: 14,007,865 (GRCm39)	C1488R	probably damaging	Het
Hjurp	G	C	1: 88,204,937 (GRCm39)		probably benign	Het
Inava	C	T	1: 136,155,288 (GRCm39)	V106I	probably benign	Het
Insr	A	G	8: 3,205,683 (GRCm39)	S1369P	probably damaging	Het
Insrr	A	C	3: 87,707,759 (GRCm39)	D67A	probably damaging	Het
Irf2	T	A	8: 47,271,886 (GRCm39)	Y158N	probably benign	Het
Katnbl1	A	G	2: 112,234,586 (GRCm39)	R23G	probably benign	Het
Lamb2	T	A	9: 108,363,936 (GRCm39)	C987*	probably null	Het
Mmp14	T	A	14: 54,676,109 (GRCm39)		probably benign	Het
Mycbpap	A	G	11: 94,402,562 (GRCm39)	Y258H	probably damaging	Het
Nav3	A	G	10: 109,602,778 (GRCm39)		probably benign	Het
Or10j7	T	C	1: 173,011,845 (GRCm39)	D52G	probably benign	Het
Or4c100	T	A	2: 88,356,507 (GRCm39)	N193K	probably damaging	Het
Parp10	T	A	15: 76,126,446 (GRCm39)	L247F	probably damaging	Het
Pcsk6	C	T	7: 65,633,451 (GRCm39)		probably benign	Het
Pgap3	A	T	11: 98,281,924 (GRCm39)	V129D	probably benign	Het
Pibf1	A	G	14: 99,377,993 (GRCm39)	Y373C	probably damaging	Het
Plcb1	A	G	2: 135,136,835 (GRCm39)	E310G	probably benign	Het
Plin3	T	C	17: 56,586,892 (GRCm39)	D385G	probably damaging	Het
Pole	A	T	5: 110,441,206 (GRCm39)	D220V	probably damaging	Het
Ptprk	T	A	10: 28,351,105 (GRCm39)	F533I	probably damaging	Het
Resf1	T	A	6: 149,229,088 (GRCm39)	D711E	probably benign	Het
Rufy1	A	T	11: 50,292,292 (GRCm39)	M499K	probably benign	Het
Scn1a	T	G	2: 66,130,119 (GRCm39)	D1232A	probably benign	Het
Sec23ip	T	C	7: 128,346,891 (GRCm39)	L49P	probably damaging	Het
Sf3b1	G	A	1: 55,039,532 (GRCm39)	Q698*	probably null	Het
Shprh	A	T	10: 11,070,116 (GRCm39)		probably null	Het
Snd1	C	A	6: 28,745,334 (GRCm39)		probably benign	Het
Stab1	C	T	14: 30,862,644 (GRCm39)	A2260T	possibly damaging	Het
Stpg2	A	G	3: 138,918,082 (GRCm39)	Q60R	probably benign	Het
Strn	T	C	17: 78,964,363 (GRCm39)	H687R	possibly damaging	Het
Tgfb3	A	T	12: 86,124,603 (GRCm39)	I35N	probably damaging	Het
Tnks2	T	C	19: 36,852,765 (GRCm39)	S166P	probably damaging	Het
Tyw5	G	A	1: 57,440,597 (GRCm39)	T55M	probably damaging	Het
Usp19	A	G	9: 108,374,369 (GRCm39)		probably null	Het
Zfp13	A	T	17: 23,795,122 (GRCm39)	I483N	probably damaging	Het

Other mutations in Lzts2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02115:Lzts2	APN	19	45,014,809 (GRCm39)	unclassified	probably benign
R0135:Lzts2	UTSW	19	45,014,626 (GRCm39)	unclassified	probably benign
R0433:Lzts2	UTSW	19	45,010,115 (GRCm39)	missense	possibly damaging	0.87
R1079:Lzts2	UTSW	19	45,011,983 (GRCm39)	missense	probably damaging	1.00
R1459:Lzts2	UTSW	19	45,009,893 (GRCm39)	missense	probably damaging	1.00
R2869:Lzts2	UTSW	19	45,012,534 (GRCm39)	nonsense	probably null
R2869:Lzts2	UTSW	19	45,012,534 (GRCm39)	nonsense	probably null
R4450:Lzts2	UTSW	19	45,012,032 (GRCm39)	nonsense	probably null
R5806:Lzts2	UTSW	19	45,014,806 (GRCm39)	unclassified	probably benign
R6958:Lzts2	UTSW	19	45,012,582 (GRCm39)	unclassified	probably benign
R7598:Lzts2	UTSW	19	45,012,272 (GRCm39)	nonsense	probably null
R9398:Lzts2	UTSW	19	45,013,208 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CAACTGAAGGAATCTCAGGCAGAGC -3'
(R):5'- TGATCTCCTCCAGACAGATGCAAGG -3'

Sequencing Primer
(F):5'- AATCTCAGGCAGAGCTGGTC -3'
(R):5'- ATGCAAGGGGCCGACTC -3'

Posted On

2013-08-06