Mutagenetix > Incidental Mutation

Incidental Mutation 'R8334:Or8g21'

644526

Institutional Source

Beutler Lab

Gene Symbol

Or8g21

Ensembl Gene

ENSMUSG00000059595

Gene Name

olfactory receptor family 8 subfamily G member 21

Synonyms

MOR171-11, GA_x6K02T2PVTD-32691280-32690354, Olfr935

MMRRC Submission

067862-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.064) question?

Stock #

R8334 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

38905803-38906729 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 38905889 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 281 (I281F)

Ref Sequence

ENSEMBL: ENSMUSP00000149193 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080748] [ENSMUST00000214157]

AlphaFold

Q8VG16

Predicted Effect

probably benign
Transcript: ENSMUST00000080748
AA Change: I281F

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000079572
Gene: ENSMUSG00000059595
AA Change: I281F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	4e-53	PFAM
Pfam:7tm_1	41	290	1.2e-22	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214157
AA Change: I281F

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 99.0%

Validation Efficiency

98% (63/64)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca8a	A	T	11: 109,959,650 (GRCm39)	S696T	probably damaging	Het
Abhd12	T	C	2: 150,700,373 (GRCm39)	I75V	probably benign	Het
Adamts9	T	C	6: 92,914,225 (GRCm39)		probably null	Het
Ap2a1	C	T	7: 44,554,135 (GRCm39)	V499I	possibly damaging	Het
Arhgef16	T	A	4: 154,367,224 (GRCm39)	K394*	probably null	Het
Armc2	A	G	10: 41,799,761 (GRCm39)	F699S	probably damaging	Het
Atat1	C	T	17: 36,220,150 (GRCm39)		probably null	Het
Atm	A	G	9: 53,433,573 (GRCm39)	S226P	probably benign	Het
Bcas3	A	G	11: 85,467,637 (GRCm39)	T687A	possibly damaging	Het
Bmp2k	T	C	5: 97,175,753 (GRCm39)	M78T	possibly damaging	Het
Brinp3	T	A	1: 146,777,791 (GRCm39)	L746H	probably damaging	Het
Capn8	G	A	1: 182,438,670 (GRCm39)		probably null	Het
Ccdc154	T	A	17: 25,390,581 (GRCm39)	F602I	probably damaging	Het
Celsr3	CGGGG	CGGGGG	9: 108,718,471 (GRCm39)		probably null	Het
Chd3	A	G	11: 69,241,622 (GRCm39)	F1504L	probably damaging	Het
Cpsf1	A	T	15: 76,487,787 (GRCm39)	N77K	probably benign	Het
Crybb3	T	A	5: 113,223,845 (GRCm39)	Q188L	possibly damaging	Het
Dnah8	T	A	17: 30,988,805 (GRCm39)	H3258Q	probably benign	Het
Dnajb6	C	T	5: 29,986,238 (GRCm39)	R269W	unknown	Het
Dusp9	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	TAAAGCGGAGGCCAAAGCGGAGGCCAAAGCGGAGGCTAAAGCGGAGGCCAAAGCGGAGGCCAAAG	X: 72,684,217 (GRCm39)		probably benign	Het
Ech1	A	G	7: 28,531,248 (GRCm39)	I279V	probably benign	Het
Ehbp1	C	A	11: 21,957,170 (GRCm39)	R1173L	probably damaging	Het
Ehd4	T	C	2: 119,967,545 (GRCm39)	E83G	probably damaging	Het
Epha7	A	G	4: 28,938,777 (GRCm39)	E544G	probably benign	Het
Esp23	A	G	17: 39,384,795 (GRCm39)	V67A	possibly damaging	Het
Etl4	T	C	2: 20,785,857 (GRCm39)	V726A	probably damaging	Het
Fbxo39	G	A	11: 72,208,470 (GRCm39)	W274*	probably null	Het
Filip1l	T	C	16: 57,390,510 (GRCm39)	I366T	probably benign	Het
Gm4841	T	C	18: 60,404,054 (GRCm39)	D13G	probably benign	Het
Gtpbp2	T	A	17: 46,477,368 (GRCm39)	F411Y	possibly damaging	Het
Kmt5b	G	T	19: 3,864,795 (GRCm39)	V620L	probably benign	Het
Lrrc24	T	C	15: 76,600,200 (GRCm39)	Q313R	probably benign	Het
Lsm1	A	G	8: 26,292,047 (GRCm39)	E108G	probably benign	Het
Macf1	T	C	4: 123,325,901 (GRCm39)	K5201E	possibly damaging	Het
Matcap2	A	G	9: 22,355,414 (GRCm39)	E483G	probably benign	Het
Mettl25b	C	T	3: 87,835,056 (GRCm39)	V31I	possibly damaging	Het
Mki67	G	T	7: 135,298,245 (GRCm39)	T2263K	probably damaging	Het
Mroh1	G	A	15: 76,330,756 (GRCm39)	G1156S	probably benign	Het
Ncor1	A	T	11: 62,274,070 (GRCm39)	M190K	probably damaging	Het
Nsmce3	A	G	7: 64,522,467 (GRCm39)	V67A	probably damaging	Het
Nuggc	T	C	14: 65,882,478 (GRCm39)	V741A	probably benign	Het
Olfm3	G	T	3: 114,916,206 (GRCm39)	L379F	probably damaging	Het
Or4x13	T	C	2: 90,231,277 (GRCm39)	S91P	probably benign	Het
Pcdhb13	A	G	18: 37,577,853 (GRCm39)	T744A	probably damaging	Het
Plek	T	C	11: 16,933,220 (GRCm39)	T298A	probably benign	Het
Pou5f2	A	G	13: 78,173,392 (GRCm39)	I111M	probably benign	Het
Pou6f2	C	T	13: 18,299,991 (GRCm39)	R556H	probably damaging	Het
Pramel58	T	G	5: 94,830,635 (GRCm39)	N44K	probably benign	Het
Rcor1	G	A	12: 111,059,529 (GRCm39)	A148T		Het
Rnh1	A	G	7: 140,748,544 (GRCm39)	V11A	probably benign	Het
Slc35f1	T	C	10: 52,984,244 (GRCm39)	F335L	possibly damaging	Het
Srrm2	T	C	17: 24,027,330 (GRCm39)	V22A	unknown	Het
St7	A	T	6: 17,934,220 (GRCm39)	H534L	probably damaging	Het
Swi5	T	A	2: 32,170,463 (GRCm39)		probably benign	Het
Syf2	T	A	4: 134,658,586 (GRCm39)	H40Q	probably benign	Het
Tgtp2	G	A	11: 48,949,721 (GRCm39)	L284F	probably benign	Het
Tnn	C	T	1: 159,946,053 (GRCm39)	G922R	probably damaging	Het
Trbc1	A	C	6: 41,516,046 (GRCm39)		probably benign	Het
Trim33	A	T	3: 103,261,145 (GRCm39)	T1115S	probably benign	Het
Ttc22	C	A	4: 106,496,115 (GRCm39)		probably null	Het
Ttn	C	T	2: 76,638,374 (GRCm39)	A13969T	probably damaging	Het
Tuba4a	T	C	1: 75,193,945 (GRCm39)	D74G	probably benign	Het
Ube3c	T	A	5: 29,795,882 (GRCm39)	D90E	probably benign	Het
Ubr7	T	C	12: 102,724,397 (GRCm39)	V37A	probably damaging	Het
Vmn2r28	A	C	7: 5,487,059 (GRCm39)	C535G	probably damaging	Het
Wdr36	A	G	18: 32,992,346 (GRCm39)	T628A	possibly damaging	Het
Whrn	C	T	4: 63,413,047 (GRCm39)	V142M	probably damaging	Het
Wnk2	A	T	13: 49,203,958 (GRCm39)		probably null	Het
Xkr7	C	T	2: 152,896,883 (GRCm39)	T579I	probably damaging	Het

Other mutations in Or8g21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01522:Or8g21	APN	9	38,906,396 (GRCm39)	missense	probably benign	0.08
IGL01671:Or8g21	APN	9	38,906,149 (GRCm39)	missense	probably benign	0.05
IGL02331:Or8g21	APN	9	38,906,402 (GRCm39)	missense	probably damaging	0.99
IGL03069:Or8g21	APN	9	38,906,728 (GRCm39)	start codon destroyed	probably null	0.98
IGL03143:Or8g21	APN	9	38,906,732 (GRCm39)	utr 5 prime	probably benign
R0149:Or8g21	UTSW	9	38,905,880 (GRCm39)	missense	probably benign	0.05
R0400:Or8g21	UTSW	9	38,906,494 (GRCm39)	missense	probably damaging	1.00
R1818:Or8g21	UTSW	9	38,905,902 (GRCm39)	missense	possibly damaging	0.61
R2092:Or8g21	UTSW	9	38,906,485 (GRCm39)	missense	probably damaging	1.00
R2151:Or8g21	UTSW	9	38,906,012 (GRCm39)	missense	probably damaging	1.00
R2166:Or8g21	UTSW	9	38,906,513 (GRCm39)	nonsense	probably null
R2347:Or8g21	UTSW	9	38,905,805 (GRCm39)	makesense	probably null
R4404:Or8g21	UTSW	9	38,905,865 (GRCm39)	missense	possibly damaging	0.77
R4406:Or8g21	UTSW	9	38,905,865 (GRCm39)	missense	possibly damaging	0.77
R5152:Or8g21	UTSW	9	38,906,473 (GRCm39)	missense	possibly damaging	0.88
R5467:Or8g21	UTSW	9	38,906,200 (GRCm39)	missense	probably benign	0.14
R5509:Or8g21	UTSW	9	38,905,924 (GRCm39)	missense	probably benign	0.03
R5954:Or8g21	UTSW	9	38,906,711 (GRCm39)	missense	probably damaging	1.00
R6647:Or8g21	UTSW	9	38,906,210 (GRCm39)	missense	possibly damaging	0.55
R6680:Or8g21	UTSW	9	38,905,954 (GRCm39)	missense	probably damaging	1.00
R6928:Or8g21	UTSW	9	38,905,928 (GRCm39)	missense	probably benign	0.03
R7242:Or8g21	UTSW	9	38,906,437 (GRCm39)	missense	probably benign	0.31
R7271:Or8g21	UTSW	9	38,905,953 (GRCm39)	nonsense	probably null
R7309:Or8g21	UTSW	9	38,906,576 (GRCm39)	missense	probably damaging	1.00
R7775:Or8g21	UTSW	9	38,906,203 (GRCm39)	missense	probably damaging	1.00
R7778:Or8g21	UTSW	9	38,906,203 (GRCm39)	missense	probably damaging	1.00
R7866:Or8g21	UTSW	9	38,906,027 (GRCm39)	missense	not run
R8280:Or8g21	UTSW	9	38,906,075 (GRCm39)	missense	probably benign
R8841:Or8g21	UTSW	9	38,905,879 (GRCm39)	missense	possibly damaging	0.95
R9161:Or8g21	UTSW	9	38,905,816 (GRCm39)	missense	possibly damaging	0.70
R9169:Or8g21	UTSW	9	38,906,573 (GRCm39)	missense	probably benign	0.23
R9659:Or8g21	UTSW	9	38,906,296 (GRCm39)	missense	possibly damaging	0.64
R9739:Or8g21	UTSW	9	38,906,302 (GRCm39)	nonsense	probably null
R9788:Or8g21	UTSW	9	38,906,296 (GRCm39)	missense	possibly damaging	0.64

Predicted Primers

PCR Primer
(F):5'- TGACCCTGGAATTGAAGACAC -3'
(R):5'- AGCATCTTACGCATTAAATCCACTG -3'

Sequencing Primer
(F):5'- CCCTGGAATTGAAGACACAAGGTG -3'
(R):5'- TTACGCATTAAATCCACTGAGGGC -3'

Posted On

2020-09-02