Mutagenetix > Incidental Mutation

Incidental Mutation 'R7949:Tnxb'

649482

Institutional Source

Beutler Lab

Gene Symbol

Tnxb

Ensembl Gene

ENSMUSG00000033327

Gene Name

tenascin XB

Synonyms

Tnx, TN-MHC

MMRRC Submission

045994-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7949 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

34879431-34938789 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 34936103 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 2680 (P2680L)

Ref Sequence

ENSEMBL: ENSMUSP00000127487 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000087399] [ENSMUST00000168533]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000087399
AA Change: P3560L

PolyPhen 2 Score 0.957 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000084661
Gene: ENSMUSG00000033327
AA Change: P3560L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	69	89	N/A	INTRINSIC
low complexity region	92	103	N/A	INTRINSIC
EGF	173	201	1.59e1	SMART
EGF	204	232	7.41e0	SMART
EGF	235	263	2.64e1	SMART
EGF	266	294	2.64e1	SMART
EGF	297	325	9.13e0	SMART
EGF	328	356	2.07e1	SMART
EGF_like	359	387	3.16e1	SMART
EGF_like	390	418	4.64e1	SMART
EGF_like	421	449	3.29e1	SMART
EGF_like	452	480	7.09e1	SMART
EGF	483	511	1.15e1	SMART
EGF	514	542	1.91e1	SMART
EGF_like	545	573	4.11e1	SMART
EGF	576	604	7.95e0	SMART
EGF	607	635	1.23e1	SMART
EGF_like	638	666	4.93e1	SMART
EGF_like	674	702	5.24e1	SMART
EGF	705	733	2.29e1	SMART
FN3	736	816	4.12e-3	SMART
FN3	827	904	1.21e-9	SMART
FN3	1047	1126	3.97e-5	SMART
FN3	1142	1223	3.62e-8	SMART
low complexity region	1230	1241	N/A	INTRINSIC
FN3	1242	1320	2.31e-6	SMART
FN3	1351	1431	8.77e-7	SMART
FN3	1460	1539	3.01e-5	SMART
FN3	1556	1636	2.76e-4	SMART
FN3	1657	1736	5.78e-7	SMART
FN3	1752	1832	4.7e-7	SMART
FN3	1851	1929	1.95e-4	SMART
FN3	1955	2034	4.56e-5	SMART
FN3	2066	2145	2.23e-8	SMART
FN3	2164	2244	7.75e-8	SMART
FN3	2279	2358	8.5e-5	SMART
FN3	2387	2467	2.94e-8	SMART
FN3	2501	2580	1.7e-4	SMART
low complexity region	2588	2598	N/A	INTRINSIC
FN3	2607	2687	6.75e-8	SMART
FN3	2716	2795	7.4e-5	SMART
FN3	2822	2902	1.35e-7	SMART
FN3	2931	3010	5.61e-5	SMART
FN3	3026	3106	6.01e-5	SMART
FN3	3120	3199	6.45e-5	SMART
FN3	3214	3293	9.54e-8	SMART
FN3	3316	3396	2.81e-5	SMART
FN3	3416	3502	1.98e-5	SMART
FN3	3518	3596	5.65e-10	SMART
FN3	3607	3682	7.63e-7	SMART
FN3	3695	3770	6.54e-6	SMART
FBG	3787	3997	8.88e-125	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000168533
AA Change: P2680L

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000127487
Gene: ENSMUSG00000033327
AA Change: P2680L

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
low complexity region	33	44	N/A	INTRINSIC
low complexity region	69	89	N/A	INTRINSIC
low complexity region	92	103	N/A	INTRINSIC
EGF	173	201	1.59e1	SMART
EGF	204	232	7.41e0	SMART
EGF	235	263	2.64e1	SMART
EGF	266	294	2.64e1	SMART
EGF	297	325	9.13e0	SMART
EGF	328	356	2.07e1	SMART
EGF_like	359	387	3.16e1	SMART
EGF_like	390	418	4.64e1	SMART
EGF_like	421	449	3.29e1	SMART
EGF_like	452	480	7.09e1	SMART
EGF	483	511	1.15e1	SMART
EGF	514	542	1.91e1	SMART
EGF_like	545	573	4.11e1	SMART
EGF	576	604	7.95e0	SMART
EGF	607	635	1.23e1	SMART
EGF_like	638	666	4.93e1	SMART
EGF_like	674	702	5.24e1	SMART
EGF	705	733	2.29e1	SMART
FN3	736	816	4.12e-3	SMART
FN3	827	904	1.21e-9	SMART
FN3	924	1003	3.97e-5	SMART
FN3	1019	1100	3.62e-8	SMART
low complexity region	1107	1118	N/A	INTRINSIC
FN3	1119	1197	2.31e-6	SMART
FN3	1228	1308	8.77e-7	SMART
FN3	1337	1416	3.01e-5	SMART
FN3	1433	1513	2.76e-4	SMART
FN3	1534	1613	5.78e-7	SMART
FN3	1629	1709	4.7e-7	SMART
FN3	1728	1806	1.95e-4	SMART
FN3	1832	1911	4.56e-5	SMART
FN3	1943	2022	2.23e-8	SMART
FN3	2051	2130	5.61e-5	SMART
FN3	2146	2226	6.01e-5	SMART
FN3	2240	2319	6.45e-5	SMART
FN3	2334	2413	9.54e-8	SMART
FN3	2436	2516	2.81e-5	SMART
FN3	2536	2622	1.98e-5	SMART
FN3	2638	2716	5.65e-10	SMART
FN3	2727	2802	7.63e-7	SMART
FN3	2815	2890	6.54e-6	SMART
FBG	2907	3117	8.88e-125	SMART

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.7%
20x: 98.9%

Validation Efficiency

100% (47/47)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous null mice have stretchy skin, similar to patients with human Ehlers-Danlos syndrome. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adgrv1	G	A	13: 81,303,353 (GRCm39)	T6111M	probably damaging	Het
Adprhl1	T	G	8: 13,274,225 (GRCm39)	R844S	possibly damaging	Het
Aldh1b1	A	G	4: 45,802,807 (GRCm39)	E115G	possibly damaging	Het
Anp32a	A	G	9: 62,280,948 (GRCm39)	E170G	unknown	Het
Arhgap27	A	G	11: 103,228,595 (GRCm39)	S349P	probably damaging	Het
Asns	T	A	6: 7,685,328 (GRCm39)	I110F	probably damaging	Het
Atad3a	T	C	4: 155,833,152 (GRCm39)	H437R	possibly damaging	Het
Atl2	C	A	17: 80,167,289 (GRCm39)	R244L	probably damaging	Het
Bcl2l14	A	T	6: 134,407,083 (GRCm39)	D222V	probably damaging	Het
Ccdc102a	G	T	8: 95,631,913 (GRCm39)	Q498K	probably damaging	Het
Cdc14b	T	C	13: 64,338,212 (GRCm39)		probably null	Het
Cdcp3	G	A	7: 130,895,324 (GRCm39)		probably null	Het
Cep162	T	A	9: 87,088,901 (GRCm39)	M994L	probably benign	Het
Ddx18	A	G	1: 121,483,047 (GRCm39)	Y580H	probably damaging	Het
Entpd6	C	A	2: 150,612,197 (GRCm39)		probably null	Het
Fer	T	A	17: 64,440,503 (GRCm39)	S707T	probably damaging	Het
Gabra6	A	G	11: 42,207,826 (GRCm39)	V215A	probably benign	Het
Gm20671	A	G	5: 32,977,288 (GRCm39)	V142A	probably benign	Het
Gtf3c1	G	T	7: 125,250,253 (GRCm39)	N1439K	probably benign	Het
Igbp1b	T	C	6: 138,635,414 (GRCm39)	K10R	probably benign	Het
Ighv5-8	TATATATATATATATATATATATA	TATATATATATATATATATATATATA	12: 113,618,563 (GRCm39)		probably null	Het
Igkv12-44	A	T	6: 69,791,874 (GRCm39)	S30T	probably benign	Het
Immt	A	T	6: 71,851,327 (GRCm39)	R563*	probably null	Het
Jakmip1	A	T	5: 37,339,492 (GRCm39)	Q1231L	probably damaging	Het
Jsrp1	T	C	10: 80,647,906 (GRCm39)	T51A	probably benign	Het
Krtap31-1	A	G	11: 99,799,144 (GRCm39)	T116A	possibly damaging	Het
Lrp8	T	A	4: 107,660,524 (GRCm39)	D61E	probably damaging	Het
Mark2	T	C	19: 7,262,081 (GRCm39)	D311G	probably benign	Het
Men1	C	T	19: 6,388,323 (GRCm39)	S314L	possibly damaging	Het
Nxpe2	T	C	9: 48,234,397 (GRCm39)	N290S	possibly damaging	Het
Or2f2	T	C	6: 42,767,588 (GRCm39)	V205A	possibly damaging	Het
Or5b105	T	A	19: 13,080,610 (GRCm39)		probably null	Het
Pcolce2	A	T	9: 95,576,688 (GRCm39)	T320S	probably benign	Het
Phc2	C	T	4: 128,603,401 (GRCm39)	T177M	probably damaging	Het
Phf21b	G	T	15: 84,676,036 (GRCm39)	P337H	probably damaging	Het
Pinlyp	G	T	7: 24,245,375 (GRCm39)	T15K	probably damaging	Het
Ppox	A	T	1: 171,105,521 (GRCm39)	S307R	probably benign	Het
Ptk7	T	A	17: 46,897,387 (GRCm39)	E315V	possibly damaging	Het
Pxk	T	C	14: 8,144,233 (GRCm38)	I327T	probably damaging	Het
Rabgap1	A	T	2: 37,453,491 (GRCm39)	K973I	probably benign	Het
Rbbp8	A	T	18: 11,851,892 (GRCm39)	I238L	probably benign	Het
Runx2	T	A	17: 45,046,442 (GRCm39)	D109V	possibly damaging	Het
Serpinb6a	G	A	13: 34,107,003 (GRCm39)	S183L	probably benign	Het
Slco1a8	C	A	6: 141,939,991 (GRCm39)	G171C	probably damaging	Het
Sptlc3	T	C	2: 139,467,795 (GRCm39)	I451T	possibly damaging	Het
Sugp1	A	T	8: 70,509,153 (GRCm39)	Y142F	possibly damaging	Het
Sumf2	T	C	5: 129,881,759 (GRCm39)	F98S	probably damaging	Het
Tmem120a	C	T	5: 135,771,220 (GRCm39)	E39K	possibly damaging	Het
Zfp78	C	G	7: 6,382,365 (GRCm39)	R472G	possibly damaging	Het

Other mutations in Tnxb

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00094:Tnxb	APN	17	34,904,603 (GRCm39)	missense	probably damaging	1.00
IGL00424:Tnxb	APN	17	34,933,666 (GRCm39)	missense	probably damaging	1.00
IGL00486:Tnxb	APN	17	34,911,356 (GRCm39)	missense	probably damaging	1.00
IGL00952:Tnxb	APN	17	34,932,102 (GRCm39)	missense	probably damaging	1.00
IGL00974:Tnxb	APN	17	34,937,707 (GRCm39)	critical splice donor site	probably null
IGL01017:Tnxb	APN	17	34,912,782 (GRCm39)	missense	probably damaging	0.98
IGL01082:Tnxb	APN	17	34,933,584 (GRCm39)	missense	probably damaging	0.97
IGL01397:Tnxb	APN	17	34,933,647 (GRCm39)	missense	probably damaging	0.99
IGL01473:Tnxb	APN	17	34,904,675 (GRCm39)	missense	probably damaging	0.99
IGL01642:Tnxb	APN	17	34,937,488 (GRCm39)	missense	probably damaging	1.00
IGL01774:Tnxb	APN	17	34,907,813 (GRCm39)	missense	probably damaging	1.00
IGL01971:Tnxb	APN	17	34,891,271 (GRCm39)	missense	probably damaging	1.00
IGL02016:Tnxb	APN	17	34,891,249 (GRCm39)	missense	probably damaging	0.98
IGL02160:Tnxb	APN	17	34,933,719 (GRCm39)	missense	probably benign	0.01
IGL02473:Tnxb	APN	17	34,936,736 (GRCm39)	missense	probably damaging	1.00
IGL02666:Tnxb	APN	17	34,903,913 (GRCm39)	missense	probably benign	0.20
IGL02831:Tnxb	APN	17	34,922,545 (GRCm39)	missense	possibly damaging	0.93
IGL02838:Tnxb	APN	17	34,908,606 (GRCm39)	missense	possibly damaging	0.74
IGL02965:Tnxb	APN	17	34,928,628 (GRCm39)	missense	possibly damaging	0.93
IGL03155:Tnxb	APN	17	34,932,569 (GRCm39)	missense	probably damaging	1.00
IGL03194:Tnxb	APN	17	34,914,921 (GRCm39)	nonsense	probably null
IGL03215:Tnxb	APN	17	34,911,499 (GRCm39)	missense	possibly damaging	0.66
IGL03256:Tnxb	APN	17	34,907,694 (GRCm39)	missense	probably damaging	1.00
BB008:Tnxb	UTSW	17	34,907,672 (GRCm39)	missense	probably damaging	1.00
BB018:Tnxb	UTSW	17	34,907,672 (GRCm39)	missense	probably damaging	1.00
E0370:Tnxb	UTSW	17	34,897,917 (GRCm39)	missense	probably damaging	1.00
R0006:Tnxb	UTSW	17	34,901,266 (GRCm39)	missense	probably benign	0.07
R0049:Tnxb	UTSW	17	34,928,542 (GRCm39)	missense	possibly damaging	0.93
R0050:Tnxb	UTSW	17	34,892,299 (GRCm39)	missense	probably damaging	1.00
R0233:Tnxb	UTSW	17	34,918,007 (GRCm39)	missense	probably benign	0.32
R0233:Tnxb	UTSW	17	34,918,007 (GRCm39)	missense	probably benign	0.32
R0311:Tnxb	UTSW	17	34,935,958 (GRCm39)	missense	probably damaging	0.97
R0326:Tnxb	UTSW	17	34,917,153 (GRCm39)	missense	probably benign	0.32
R0387:Tnxb	UTSW	17	34,902,548 (GRCm39)	missense	probably benign	0.30
R0396:Tnxb	UTSW	17	34,890,707 (GRCm39)	missense	probably damaging	1.00
R0511:Tnxb	UTSW	17	34,937,219 (GRCm39)	missense	probably damaging	0.96
R0540:Tnxb	UTSW	17	34,890,892 (GRCm39)	missense	probably damaging	1.00
R0563:Tnxb	UTSW	17	34,935,921 (GRCm39)	missense	probably benign	0.05
R0575:Tnxb	UTSW	17	34,936,180 (GRCm39)	missense	possibly damaging	0.91
R0586:Tnxb	UTSW	17	34,891,118 (GRCm39)	missense	probably damaging	1.00
R0607:Tnxb	UTSW	17	34,890,892 (GRCm39)	missense	probably damaging	1.00
R0622:Tnxb	UTSW	17	34,937,703 (GRCm39)	missense	probably damaging	1.00
R0624:Tnxb	UTSW	17	34,902,522 (GRCm39)	missense	probably damaging	1.00
R0709:Tnxb	UTSW	17	34,908,328 (GRCm39)	missense	probably damaging	1.00
R0898:Tnxb	UTSW	17	34,889,719 (GRCm39)	missense	probably damaging	1.00
R0970:Tnxb	UTSW	17	34,917,917 (GRCm39)	missense	possibly damaging	0.85
R0972:Tnxb	UTSW	17	34,904,117 (GRCm39)	missense	probably damaging	1.00
R1118:Tnxb	UTSW	17	34,904,017 (GRCm39)	missense	probably damaging	1.00
R1119:Tnxb	UTSW	17	34,904,017 (GRCm39)	missense	probably damaging	1.00
R1226:Tnxb	UTSW	17	34,907,903 (GRCm39)	missense	probably damaging	1.00
R1296:Tnxb	UTSW	17	34,890,551 (GRCm39)	missense	probably damaging	1.00
R1297:Tnxb	UTSW	17	34,929,140 (GRCm39)	missense	probably damaging	0.96
R1349:Tnxb	UTSW	17	34,929,267 (GRCm39)	missense	possibly damaging	0.67
R1356:Tnxb	UTSW	17	34,914,446 (GRCm39)	missense	possibly damaging	0.53
R1372:Tnxb	UTSW	17	34,929,267 (GRCm39)	missense	possibly damaging	0.67
R1521:Tnxb	UTSW	17	34,930,477 (GRCm39)	missense	probably damaging	1.00
R1522:Tnxb	UTSW	17	34,937,612 (GRCm39)	missense	probably damaging	1.00
R1532:Tnxb	UTSW	17	34,929,804 (GRCm39)	missense	probably damaging	1.00
R1735:Tnxb	UTSW	17	34,936,944 (GRCm39)	missense	probably damaging	1.00
R1778:Tnxb	UTSW	17	34,902,548 (GRCm39)	missense	probably benign	0.30
R1802:Tnxb	UTSW	17	34,922,863 (GRCm39)	missense	probably damaging	0.98
R1824:Tnxb	UTSW	17	34,911,307 (GRCm39)	nonsense	probably null
R1838:Tnxb	UTSW	17	34,897,884 (GRCm39)	missense	probably damaging	0.96
R1863:Tnxb	UTSW	17	34,889,848 (GRCm39)	missense	probably damaging	1.00
R1865:Tnxb	UTSW	17	34,922,431 (GRCm39)	nonsense	probably null
R1867:Tnxb	UTSW	17	34,890,821 (GRCm39)	missense	probably damaging	1.00
R1883:Tnxb	UTSW	17	34,908,539 (GRCm39)	missense	probably benign	0.01
R1884:Tnxb	UTSW	17	34,908,539 (GRCm39)	missense	probably benign	0.01
R1889:Tnxb	UTSW	17	34,914,799 (GRCm39)	missense	probably damaging	0.97
R1969:Tnxb	UTSW	17	34,898,055 (GRCm39)	missense	probably benign	0.20
R1989:Tnxb	UTSW	17	34,912,859 (GRCm39)	missense	probably damaging	1.00
R1989:Tnxb	UTSW	17	34,902,351 (GRCm39)	missense	probably benign	0.08
R1991:Tnxb	UTSW	17	34,901,225 (GRCm39)	missense	probably damaging	1.00
R1991:Tnxb	UTSW	17	34,890,878 (GRCm39)	missense	probably damaging	1.00
R1992:Tnxb	UTSW	17	34,890,878 (GRCm39)	missense	probably damaging	1.00
R2001:Tnxb	UTSW	17	34,911,553 (GRCm39)	missense	possibly damaging	0.82
R2018:Tnxb	UTSW	17	34,890,724 (GRCm39)	missense	probably benign	0.04
R2030:Tnxb	UTSW	17	34,937,443 (GRCm39)	missense	probably damaging	1.00
R2037:Tnxb	UTSW	17	34,918,179 (GRCm39)	missense	probably damaging	1.00
R2103:Tnxb	UTSW	17	34,901,225 (GRCm39)	missense	probably damaging	1.00
R2116:Tnxb	UTSW	17	34,891,201 (GRCm39)	missense	probably damaging	1.00
R2206:Tnxb	UTSW	17	34,928,391 (GRCm39)	missense	possibly damaging	0.86
R2207:Tnxb	UTSW	17	34,928,391 (GRCm39)	missense	possibly damaging	0.86
R2215:Tnxb	UTSW	17	34,923,114 (GRCm39)	missense	possibly damaging	0.93
R2413:Tnxb	UTSW	17	34,937,252 (GRCm39)	missense	probably damaging	0.99
R2680:Tnxb	UTSW	17	34,922,594 (GRCm39)	missense	possibly damaging	0.51
R2910:Tnxb	UTSW	17	34,891,424 (GRCm39)	missense	probably damaging	1.00
R2984:Tnxb	UTSW	17	34,897,636 (GRCm39)	nonsense	probably null
R3120:Tnxb	UTSW	17	34,911,329 (GRCm39)	missense	possibly damaging	0.86
R3429:Tnxb	UTSW	17	34,922,561 (GRCm39)	missense	probably damaging	0.98
R3429:Tnxb	UTSW	17	34,891,605 (GRCm39)	nonsense	probably null
R3552:Tnxb	UTSW	17	34,937,695 (GRCm39)	missense	probably damaging	1.00
R3698:Tnxb	UTSW	17	34,909,407 (GRCm39)	critical splice donor site	probably null
R3720:Tnxb	UTSW	17	34,931,938 (GRCm39)	missense	possibly damaging	0.95
R3841:Tnxb	UTSW	17	34,917,897 (GRCm39)	missense	possibly damaging	0.72
R3848:Tnxb	UTSW	17	34,909,369 (GRCm39)	missense	possibly damaging	0.82
R3886:Tnxb	UTSW	17	34,937,885 (GRCm39)	missense	probably damaging	1.00
R4074:Tnxb	UTSW	17	34,890,845 (GRCm39)	missense	probably benign	0.22
R4159:Tnxb	UTSW	17	34,930,491 (GRCm39)	missense	probably damaging	0.99
R4160:Tnxb	UTSW	17	34,930,491 (GRCm39)	missense	probably damaging	0.99
R4161:Tnxb	UTSW	17	34,930,491 (GRCm39)	missense	probably damaging	0.99
R4181:Tnxb	UTSW	17	34,928,428 (GRCm39)	missense	possibly damaging	0.93
R4210:Tnxb	UTSW	17	34,929,951 (GRCm39)	missense	possibly damaging	0.84
R4275:Tnxb	UTSW	17	34,917,205 (GRCm39)	missense	probably damaging	0.98
R4329:Tnxb	UTSW	17	34,912,838 (GRCm39)	missense	probably damaging	1.00
R4394:Tnxb	UTSW	17	34,897,636 (GRCm39)	nonsense	probably null
R4395:Tnxb	UTSW	17	34,897,636 (GRCm39)	nonsense	probably null
R4397:Tnxb	UTSW	17	34,897,636 (GRCm39)	nonsense	probably null
R4540:Tnxb	UTSW	17	34,922,309 (GRCm39)	missense	possibly damaging	0.86
R4673:Tnxb	UTSW	17	34,891,514 (GRCm39)	missense	probably damaging	0.99
R4719:Tnxb	UTSW	17	34,908,394 (GRCm39)	missense	probably damaging	1.00
R4725:Tnxb	UTSW	17	34,918,041 (GRCm39)	missense	probably damaging	0.99
R4753:Tnxb	UTSW	17	34,914,909 (GRCm39)	missense	possibly damaging	0.71
R4777:Tnxb	UTSW	17	34,890,917 (GRCm39)	missense	probably damaging	1.00
R4837:Tnxb	UTSW	17	34,936,981 (GRCm39)	missense	probably damaging	0.98
R4898:Tnxb	UTSW	17	34,914,566 (GRCm39)	missense	possibly damaging	0.95
R4938:Tnxb	UTSW	17	34,932,606 (GRCm39)	missense	probably damaging	1.00
R5044:Tnxb	UTSW	17	34,936,457 (GRCm39)	missense	probably damaging	1.00
R5100:Tnxb	UTSW	17	34,929,902 (GRCm39)	missense	probably damaging	0.99
R5223:Tnxb	UTSW	17	34,923,052 (GRCm39)	missense	possibly damaging	0.51
R5269:Tnxb	UTSW	17	34,922,582 (GRCm39)	missense	possibly damaging	0.95
R5333:Tnxb	UTSW	17	34,909,205 (GRCm39)	missense	probably damaging	1.00
R5454:Tnxb	UTSW	17	34,928,599 (GRCm39)	missense	possibly damaging	0.71
R5470:Tnxb	UTSW	17	34,935,947 (GRCm39)	missense	probably null	1.00
R5475:Tnxb	UTSW	17	34,908,567 (GRCm39)	missense	probably damaging	1.00
R5574:Tnxb	UTSW	17	34,929,998 (GRCm39)	missense	probably benign
R5596:Tnxb	UTSW	17	34,907,778 (GRCm39)	missense	probably damaging	1.00
R5599:Tnxb	UTSW	17	34,909,179 (GRCm39)	missense	probably benign	0.22
R5599:Tnxb	UTSW	17	34,909,176 (GRCm39)	missense	probably damaging	1.00
R5615:Tnxb	UTSW	17	34,902,392 (GRCm39)	missense	probably damaging	1.00
R5620:Tnxb	UTSW	17	34,936,504 (GRCm39)	nonsense	probably null
R5625:Tnxb	UTSW	17	34,904,185 (GRCm39)	missense	probably benign	0.30
R5734:Tnxb	UTSW	17	34,917,884 (GRCm39)	missense	possibly damaging	0.53
R5896:Tnxb	UTSW	17	34,891,126 (GRCm39)	missense	probably damaging	1.00
R5961:Tnxb	UTSW	17	34,937,609 (GRCm39)	missense	probably damaging	1.00
R5974:Tnxb	UTSW	17	34,904,681 (GRCm39)	missense	probably damaging	1.00
R6091:Tnxb	UTSW	17	34,929,338 (GRCm39)	missense	probably damaging	0.98
R6134:Tnxb	UTSW	17	34,890,986 (GRCm39)	missense	probably damaging	0.96
R6325:Tnxb	UTSW	17	34,911,398 (GRCm39)	missense	probably damaging	1.00
R6358:Tnxb	UTSW	17	34,897,968 (GRCm39)	missense	probably damaging	0.98
R6362:Tnxb	UTSW	17	34,913,362 (GRCm39)	missense	probably damaging	1.00
R6432:Tnxb	UTSW	17	34,936,891 (GRCm39)	missense	probably damaging	1.00
R6461:Tnxb	UTSW	17	34,890,872 (GRCm39)	missense	probably damaging	1.00
R6467:Tnxb	UTSW	17	34,912,898 (GRCm39)	missense	probably damaging	1.00
R6476:Tnxb	UTSW	17	34,909,166 (GRCm39)	missense	probably damaging	0.98
R6477:Tnxb	UTSW	17	34,938,513 (GRCm39)	missense	probably damaging	1.00
R6631:Tnxb	UTSW	17	34,937,222 (GRCm39)	missense	probably damaging	1.00
R6774:Tnxb	UTSW	17	34,928,606 (GRCm39)	nonsense	probably null
R6787:Tnxb	UTSW	17	34,929,710 (GRCm39)	missense	probably benign	0.02
R6805:Tnxb	UTSW	17	34,917,127 (GRCm39)	missense	possibly damaging	0.93
R6860:Tnxb	UTSW	17	34,932,131 (GRCm39)	missense	probably damaging	0.99
R6883:Tnxb	UTSW	17	34,937,493 (GRCm39)	missense	probably damaging	1.00
R7049:Tnxb	UTSW	17	34,936,242 (GRCm39)	critical splice donor site	probably null
R7107:Tnxb	UTSW	17	34,890,314 (GRCm39)	missense	unknown
R7172:Tnxb	UTSW	17	34,914,994 (GRCm39)	missense	probably damaging	1.00
R7206:Tnxb	UTSW	17	34,923,075 (GRCm39)	missense	possibly damaging	0.71
R7219:Tnxb	UTSW	17	34,898,039 (GRCm39)	missense	probably benign	0.08
R7237:Tnxb	UTSW	17	34,901,170 (GRCm39)	missense	possibly damaging	0.82
R7257:Tnxb	UTSW	17	34,935,475 (GRCm39)	missense	probably benign	0.44
R7269:Tnxb	UTSW	17	34,914,428 (GRCm39)	missense	probably damaging	1.00
R7302:Tnxb	UTSW	17	34,897,875 (GRCm39)	missense	probably benign	0.41
R7372:Tnxb	UTSW	17	34,936,228 (GRCm39)	missense	possibly damaging	0.72
R7384:Tnxb	UTSW	17	34,937,492 (GRCm39)	missense	probably damaging	1.00
R7447:Tnxb	UTSW	17	34,937,444 (GRCm39)	missense	probably damaging	1.00
R7449:Tnxb	UTSW	17	34,922,335 (GRCm39)	missense	possibly damaging	0.93
R7480:Tnxb	UTSW	17	34,934,747 (GRCm39)	missense	probably damaging	0.96
R7506:Tnxb	UTSW	17	34,934,665 (GRCm39)	missense	possibly damaging	0.89
R7586:Tnxb	UTSW	17	34,935,382 (GRCm39)	missense	probably damaging	0.98
R7688:Tnxb	UTSW	17	34,890,880 (GRCm39)	missense	probably benign	0.23
R7690:Tnxb	UTSW	17	34,908,494 (GRCm39)	missense	probably benign	0.03
R7690:Tnxb	UTSW	17	34,908,501 (GRCm39)	missense	probably damaging	1.00
R7732:Tnxb	UTSW	17	34,913,254 (GRCm39)	missense	probably damaging	1.00
R7735:Tnxb	UTSW	17	34,890,398 (GRCm39)	missense	unknown
R7760:Tnxb	UTSW	17	34,931,911 (GRCm39)	missense	probably damaging	0.96
R7874:Tnxb	UTSW	17	34,930,417 (GRCm39)	missense	probably damaging	1.00
R7909:Tnxb	UTSW	17	34,911,428 (GRCm39)	missense	probably benign	0.02
R7922:Tnxb	UTSW	17	34,933,577 (GRCm39)	missense	probably damaging	1.00
R7931:Tnxb	UTSW	17	34,907,672 (GRCm39)	missense	probably damaging	1.00
R7953:Tnxb	UTSW	17	34,929,077 (GRCm39)	missense	probably benign	0.03
R7953:Tnxb	UTSW	17	34,928,509 (GRCm39)	missense	possibly damaging	0.86
R7977:Tnxb	UTSW	17	34,929,194 (GRCm39)	missense	possibly damaging	0.92
R7985:Tnxb	UTSW	17	34,935,984 (GRCm39)	critical splice donor site	probably null
R7987:Tnxb	UTSW	17	34,929,194 (GRCm39)	missense	possibly damaging	0.92
R8040:Tnxb	UTSW	17	34,935,532 (GRCm39)	missense	probably damaging	1.00
R8053:Tnxb	UTSW	17	34,923,153 (GRCm39)	missense	probably damaging	0.98
R8074:Tnxb	UTSW	17	34,922,955 (GRCm39)	missense	probably benign	0.32
R8089:Tnxb	UTSW	17	34,891,763 (GRCm39)	missense	unknown
R8169:Tnxb	UTSW	17	34,918,181 (GRCm39)	missense	possibly damaging	0.96
R8348:Tnxb	UTSW	17	34,929,102 (GRCm39)	missense	possibly damaging	0.92
R8352:Tnxb	UTSW	17	34,908,381 (GRCm39)	missense	probably damaging	1.00
R8362:Tnxb	UTSW	17	34,931,946 (GRCm39)	missense	probably damaging	0.99
R8452:Tnxb	UTSW	17	34,908,381 (GRCm39)	missense	probably damaging	1.00
R8527:Tnxb	UTSW	17	34,907,634 (GRCm39)	missense	probably damaging	1.00
R8754:Tnxb	UTSW	17	34,934,882 (GRCm39)	missense	probably damaging	1.00
R8813:Tnxb	UTSW	17	34,938,136 (GRCm39)	missense	probably damaging	1.00
R8936:Tnxb	UTSW	17	34,904,646 (GRCm39)	missense	probably damaging	1.00
R8986:Tnxb	UTSW	17	34,897,646 (GRCm39)	missense	possibly damaging	0.66
R9001:Tnxb	UTSW	17	34,922,410 (GRCm39)	missense	probably benign	0.32
R9215:Tnxb	UTSW	17	34,891,564 (GRCm39)	missense	unknown
R9226:Tnxb	UTSW	17	34,904,766 (GRCm39)	missense	probably damaging	1.00
R9276:Tnxb	UTSW	17	34,929,134 (GRCm39)	missense	possibly damaging	0.60
R9279:Tnxb	UTSW	17	34,898,088 (GRCm39)	missense	possibly damaging	0.46
R9363:Tnxb	UTSW	17	34,917,294 (GRCm39)	missense	possibly damaging	0.93
R9367:Tnxb	UTSW	17	34,931,993 (GRCm39)	missense	probably damaging	1.00
R9494:Tnxb	UTSW	17	34,904,796 (GRCm39)	missense	probably damaging	1.00
R9606:Tnxb	UTSW	17	34,914,578 (GRCm39)	missense	possibly damaging	0.82
R9650:Tnxb	UTSW	17	34,930,629 (GRCm39)	missense	probably damaging	0.99
R9677:Tnxb	UTSW	17	34,917,878 (GRCm39)	missense	possibly damaging	0.93
R9690:Tnxb	UTSW	17	34,936,171 (GRCm39)	missense	probably damaging	1.00
R9761:Tnxb	UTSW	17	34,903,987 (GRCm39)	missense	probably benign	0.32
X0004:Tnxb	UTSW	17	34,922,389 (GRCm39)	missense	possibly damaging	0.71
X0010:Tnxb	UTSW	17	34,890,908 (GRCm39)	missense	probably damaging	1.00
X0019:Tnxb	UTSW	17	34,913,163 (GRCm39)	missense	possibly damaging	0.51
X0063:Tnxb	UTSW	17	34,922,482 (GRCm39)	missense	probably damaging	0.98
X0064:Tnxb	UTSW	17	34,913,006 (GRCm39)	missense	probably damaging	1.00
Z1177:Tnxb	UTSW	17	34,937,700 (GRCm39)	missense	probably damaging	0.99
Z1177:Tnxb	UTSW	17	34,902,305 (GRCm39)	missense	probably damaging	1.00
Z1177:Tnxb	UTSW	17	34,890,740 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- CCAAAGTCAAATGGGTGCCG -3'
(R):5'- CAAGTTCAATGCCCGTAGCTG -3'

Sequencing Primer
(F):5'- TCAAATGGGTGCCGCCAAC -3'
(R):5'- AGCTCAGAGGCATCCCTAG -3'

Posted On

2020-09-15