Mutagenetix > Incidental Mutation

Incidental Mutation 'R0743:Or8h10'

70757

Institutional Source

Beutler Lab

Gene Symbol

Or8h10

Ensembl Gene

ENSMUSG00000070875

Gene Name

olfactory receptor family 8 subfamily H member 10

Synonyms

Olfr1100, GA_x6K02T2Q125-48465387-48464422, MOR206-4

MMRRC Submission

038924-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R0743 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86808173-86809138 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 86808843 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Isoleucine at position 99 (T99I)

Ref Sequence

ENSEMBL: ENSMUSP00000151088 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094913] [ENSMUST00000117211]

AlphaFold

Q8VFM1

Predicted Effect

probably benign
Transcript: ENSMUST00000094913
AA Change: T99I

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000092517
Gene: ENSMUSG00000070875
AA Change: T99I

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.7e-51	PFAM
Pfam:7tm_1	41	314	2.3e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117211
AA Change: T99I

PolyPhen 2 Score 0.117 (Sensitivity: 0.93; Specificity: 0.86)

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 96.7%
20x: 92.7%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc4	A	T	14: 118,790,700 (GRCm39)	I844N	possibly damaging	Het
Bend7	A	T	2: 4,749,055 (GRCm39)	K57N	probably damaging	Het
Cacna2d4	C	T	6: 119,284,247 (GRCm39)	R745W	probably damaging	Het
Cfap91	A	G	16: 38,155,996 (GRCm39)	F76L	probably damaging	Het
Csmd2	A	T	4: 128,007,469 (GRCm39)	T149S	probably benign	Het
Cyp2a12	T	C	7: 26,731,967 (GRCm39)	I236T	probably benign	Het
Dennd2b	A	G	7: 109,156,552 (GRCm39)	L66P	probably damaging	Het
Dnase1l2	A	G	17: 24,660,854 (GRCm39)	V170A	possibly damaging	Het
Dnm2	T	A	9: 21,411,561 (GRCm39)	Y597N	probably damaging	Het
Epsti1	A	T	14: 78,168,715 (GRCm39)	R117S	probably damaging	Het
Gabarapl2	A	T	8: 112,669,137 (GRCm39)	I32F	probably damaging	Het
Glrb	T	A	3: 80,786,987 (GRCm39)	I59F	probably damaging	Het
Gosr1	A	G	11: 76,620,972 (GRCm39)	I239T	probably benign	Het
Kif5b	G	T	18: 6,209,192 (GRCm39)	R857S	probably damaging	Het
Kmt5a	C	A	5: 124,585,282 (GRCm39)	N44K	probably damaging	Het
Ksr1	A	T	11: 78,912,329 (GRCm39)	H675Q	possibly damaging	Het
Mep1b	A	G	18: 21,213,515 (GRCm39)	D68G	possibly damaging	Het
Nebl	A	C	2: 17,415,929 (GRCm39)	S327A	probably benign	Het
Nfat5	G	A	8: 108,094,698 (GRCm39)	E962K	probably damaging	Het
Nfatc4	A	C	14: 56,064,101 (GRCm39)	D126A	probably damaging	Het
Nmt2	A	T	2: 3,315,822 (GRCm39)	R271*	probably null	Het
Nol7	G	A	13: 43,554,091 (GRCm39)	V133I	probably benign	Het
Npepps	A	G	11: 97,096,884 (GRCm39)		probably benign	Het
Nphp3	GCATCATCATCATCATC	GCATCATCATCATC	9: 103,899,967 (GRCm39)		probably benign	Het
Or1e1c	A	T	11: 73,265,715 (GRCm39)	I47F	probably benign	Het
Or51ag1	C	T	7: 103,156,069 (GRCm39)	W28*	probably null	Het
Or6c66	T	A	10: 129,461,712 (GRCm39)	T73S	probably benign	Het
Ovgp1	T	A	3: 105,882,248 (GRCm39)	L37H	probably damaging	Het
Padi3	G	T	4: 140,513,740 (GRCm39)	A646D	probably benign	Het
Pamr1	A	G	2: 102,440,252 (GRCm39)	E142G	probably damaging	Het
Papolg	A	T	11: 23,820,818 (GRCm39)		probably null	Het
Pate8	T	C	9: 36,492,597 (GRCm39)	S103G	probably benign	Het
Pfkl	C	T	10: 77,831,077 (GRCm39)		probably null	Het
Plrg1	T	C	3: 82,967,224 (GRCm39)	S132P	probably benign	Het
Pramel23	A	T	4: 143,425,134 (GRCm39)	I103N	probably damaging	Het
Prr14l	C	A	5: 32,988,538 (GRCm39)	C319F	possibly damaging	Het
Prtn3	T	A	10: 79,715,511 (GRCm39)	M1K	probably null	Het
Ptpn22	T	C	3: 103,809,487 (GRCm39)	F700S	probably damaging	Het
Ptprz1	C	T	6: 23,044,366 (GRCm39)	Q1273*	probably null	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Ryr2	T	C	13: 11,569,415 (GRCm39)	D4963G	probably damaging	Het
Sec16a	G	A	2: 26,309,734 (GRCm39)	L2091F	possibly damaging	Het
Senp6	C	T	9: 80,000,871 (GRCm39)	R27C	probably damaging	Het
Shcbp1	T	A	8: 4,814,906 (GRCm39)	M191L	probably benign	Het
Sirt4	T	C	5: 115,621,014 (GRCm39)	K53E	probably benign	Het
Slc10a2	A	G	8: 5,139,132 (GRCm39)	S271P	probably damaging	Het
Slc35b2	T	A	17: 45,877,751 (GRCm39)	F293I	probably damaging	Het
Slc38a10	C	T	11: 120,031,469 (GRCm39)	V103M	probably damaging	Het
Stab2	T	A	10: 86,723,759 (GRCm39)	I1479F	probably damaging	Het
Synpo2	A	G	3: 122,906,355 (GRCm39)	V987A	probably benign	Het
Syt9	A	G	7: 107,035,768 (GRCm39)	I262V	probably damaging	Het
Taf2	GCTTCTTCTTCTTCTTCTT	GCTTCTTCTTCTTCTT	15: 54,879,857 (GRCm39)		probably benign	Het
Tmem39a	A	T	16: 38,405,764 (GRCm39)	I200F	probably damaging	Het
Ttn	G	A	2: 76,579,613 (GRCm39)	T23760M	probably damaging	Het
Uqcrc1	C	T	9: 108,773,773 (GRCm39)	Q22*	probably null	Het
Wdtc1	A	G	4: 133,027,972 (GRCm39)	W377R	probably damaging	Het
Zfp454	A	G	11: 50,764,764 (GRCm39)	S223P	probably benign	Het

Other mutations in Or8h10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02387:Or8h10	APN	2	86,808,193 (GRCm39)	nonsense	probably null
R0102:Or8h10	UTSW	2	86,808,549 (GRCm39)	missense	possibly damaging	0.90
R0477:Or8h10	UTSW	2	86,808,567 (GRCm39)	missense	probably damaging	0.99
R0586:Or8h10	UTSW	2	86,809,126 (GRCm39)	missense	probably damaging	0.99
R1163:Or8h10	UTSW	2	86,809,020 (GRCm39)	missense	probably damaging	1.00
R1640:Or8h10	UTSW	2	86,808,963 (GRCm39)	missense	probably damaging	1.00
R2865:Or8h10	UTSW	2	86,808,805 (GRCm39)	missense	possibly damaging	0.56
R4478:Or8h10	UTSW	2	86,808,562 (GRCm39)	missense	probably benign
R4548:Or8h10	UTSW	2	86,809,014 (GRCm39)	missense	probably damaging	0.98
R4618:Or8h10	UTSW	2	86,808,618 (GRCm39)	missense	possibly damaging	0.87
R4829:Or8h10	UTSW	2	86,808,918 (GRCm39)	missense	probably damaging	0.99
R4858:Or8h10	UTSW	2	86,808,693 (GRCm39)	missense	probably damaging	1.00
R5071:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5072:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5073:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5074:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5574:Or8h10	UTSW	2	86,808,867 (GRCm39)	missense	probably benign	0.00
R5735:Or8h10	UTSW	2	86,809,044 (GRCm39)	missense	probably benign	0.08
R5874:Or8h10	UTSW	2	86,808,786 (GRCm39)	missense	probably damaging	0.99
R6228:Or8h10	UTSW	2	86,809,035 (GRCm39)	missense	probably damaging	1.00
R6689:Or8h10	UTSW	2	86,808,498 (GRCm39)	missense	probably benign	0.36
R6979:Or8h10	UTSW	2	86,808,577 (GRCm39)	missense	probably damaging	1.00
R7006:Or8h10	UTSW	2	86,808,303 (GRCm39)	missense	probably damaging	0.99
R7274:Or8h10	UTSW	2	86,808,867 (GRCm39)	missense	probably benign	0.00
R7399:Or8h10	UTSW	2	86,808,501 (GRCm39)	missense	probably benign	0.19
R7560:Or8h10	UTSW	2	86,809,122 (GRCm39)	missense	probably benign	0.00
R7765:Or8h10	UTSW	2	86,808,538 (GRCm39)	missense	probably damaging	0.98
R8381:Or8h10	UTSW	2	86,808,373 (GRCm39)	missense	probably benign	0.36
R8390:Or8h10	UTSW	2	86,808,501 (GRCm39)	missense	probably benign	0.19
R8694:Or8h10	UTSW	2	86,808,591 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GAAAACAGAAGCCACTTGTTCCTTTCC -3'
(R):5'- CGCAGGCATGATGCTGATCATTCG -3'

Sequencing Primer
(F):5'- TGTATCACTGCAAGACAGTGC -3'
(R):5'- TAGATGCTCAGCTTCACACTCC -3'

Posted On

2013-09-30